Determination of the concentration of protein by dry weight--a comparison with spectrophotometric methods.

abstract：:The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kineti...

journal_title：Archives of biochemistry and biophysics

authors： Jennings IG,Cotton RG,Kobe B

更新日期：2000-12-15 00:00:00

abstract：:Sphingosine kinase 1 (SphK1) responds to a variety of growth factor signals by increasing catalytic activity as it translocates to the plasma membrane (PM). Several studies have identified amino acids residues involved in translocation yet how SphK1 increases its catalytic activity remains to be elucidated. Herein, we...

journal_title：Archives of biochemistry and biophysics

authors： Hengst JA,Guilford JM,Conroy EJ,Wang X,Yun JK

更新日期：2010-02-01 00:00:00

abstract：:Nitric oxide formation by GH3 pituitary cells is stimulated by depolarizing concentrations of K+ and by the L-channel Ca2+ agonist Bay kappa 8644 in an additive manner that depends on extracellular Ca2+. Ca(2+)-dependent NO formation at 100 microM arginine was inhibited 50% over a 30-min period by 5 microM NG-amino-L-...

journal_title：Archives of biochemistry and biophysics

authors： Cooper GR,Barr A,Wolff DJ

更新日期：1998-09-15 00:00:00

abstract：BACKGROUND:This study was designed to identify novel links between lipid species and disease progression in non-alcoholic fatty liver disease (NAFLD). METHODS:We analyzed lipid species in the liver and plasma of db/db mice fed a choline-deficient l-amino acid-defined, high-fat diet (CDAHFD) using liquid chromatography...

journal_title：Archives of biochemistry and biophysics

authors： Horas H Nababan S,Nishiumi S,Kawano Y,Kobayashi T,Yoshida M,Azuma T

更新日期：2017-06-01 00:00:00

abstract：:A wide occurrence of peptide:N-glycanase (PNGase) in mouse organs was demonstrated. PNGase activities were determined using 14C-labeled fetuin glycopeptide I as a substrate by a newly improved enzyme assay based on the paper chromatographic and paper electrophoretic analyses. PNGase activities were detected in both so...

journal_title：Archives of biochemistry and biophysics

authors： Kitajima K,Suzuki T,Kouchi Z,Inoue S,Inoue Y

更新日期：1995-06-01 00:00:00

abstract：:Thyroid peroxidase (TPO) simultaneously catalyzes two very different types of reaction in the thyroid gland- iodination and coupling. The present study addresses the mechanism of this simultaneous dual activity. Compound I, the two-electron oxidation product of TPO, exists in two different forms--an oxoferryl porphyri...

journal_title：Archives of biochemistry and biophysics

authors： Taurog A,Dorris ML,Doerge DR

更新日期：1996-06-01 00:00:00

abstract：:The chemical structures of the carotenoid molecules are very simple and one might think that their electronic features are easily predicted. However, there is still has so much unknown information excepting the correlation between the electronic energy state and the length of effective conjugation chain of carotenoids...

journal_title：Archives of biochemistry and biophysics

authors： Uragami C,Saito K,Yoshizawa M,Molnár P,Hashimoto H

更新日期：2018-07-15 00:00:00

abstract：:The modes of action of a Vero toxin (VT2 or Shiga-like toxin II) from Escherichia coli, of ricin, and of alpha-sarcin were compared. Elongation factor 1 (EF1) and GTP-dependent Phe-tRNA binding to ribosomes in the presence of poly(U) was inhibited by these three toxins, but EF1 and guanylyl (beta, gamma-methylene)-dip...

journal_title：Archives of biochemistry and biophysics

authors： Furutani M,Kashiwagi K,Ito K,Endo Y,Igarashi K

更新日期：1992-02-14 00:00:00

abstract：:Formation of DNA-protein cross-links between thymine and tyrosine in chromatin of gamma-irradiated or H2O2-treated cultured human cells is reported. Chromatin was isolated from cells, and subsequently hydrolyzed and derivatized. Analysis of derivatized hydrolysates by gas chromatography/mass spectrometry with selected...

journal_title：Archives of biochemistry and biophysics

authors： Olinski R,Nackerdien Z,Dizdaroglu M

更新日期：1992-08-15 00:00:00

abstract：:Chloroplast tRNA(Glu) is a bifunctional molecule involved in both the early steps of chlorophyll synthesis and chloroplast protein biosynthesis. Recently the enzymes involved in these processes have been characterized from the green alga Chlamydomonas reinhardtii. In order to investigate whether transcription of the g...

journal_title：Archives of biochemistry and biophysics

authors： Jahn D

更新日期：1992-11-01 00:00:00

abstract：:The parent pyridine nucleotide NAD is the end product of oxidative tryptophan catabolism via the kynurenine pathway. Indoleamine 2, 3-dioxygenase, the rate-limiting enzyme for this pathway, is induced by the proinflammatory cytokine interferon-gamma. The aim of this study was to investigate the effect of interferon-ga...

journal_title：Archives of biochemistry and biophysics

authors： Grant RS,Passey R,Matanovic G,Smythe G,Kapoor V

更新日期：1999-12-01 00:00:00

abstract：:The steady-state kinetic mechanism of vitamin K-dependent carboxylase from calf liver has been investigated by initial-velocity measurements with varying concentrations of two carboxylase substrates and constant, nonsaturating concentrations of the other two substrates. With all combinations of the varied substrates t...

journal_title：Archives of biochemistry and biophysics

authors： Uotila L

更新日期：1988-07-01 00:00:00

abstract：:Vascular dysfunction and injurious stimuli such as oxidative stress is closely related to the risk of cardiovascular diseases (CVD). Dietary polyphenols is reported to exert the beneficial effects on reducing the risk of CVD. Black soybean is rich in polyphenols, including isoflavones, anthocyanidins and flavan-3-ols,...

journal_title：Archives of biochemistry and biophysics

authors： Yamashita Y,Wang L,Nakamura A,Nanba F,Saito S,Toda T,Nakagawa J,Ashida H

更新日期：2020-07-30 00:00:00

abstract：:Prostaglandin endoperoxide H synthases-1 and -2 (PGHS-1 and -2) are integral membrane proteins associated with the luminal surface of the endoplasmic reticulum (ER) and the nuclear envelope (NE). The C-terminal sequences of PGHSs end in -Ser/Pro-Thr-Glu-Leu (-S/PTEL), a sequence similar to one of the known ER targetin...

journal_title：Archives of biochemistry and biophysics

authors： Song I,Smith WL

更新日期：1996-10-01 00:00:00

abstract：:The P-III class of venom metalloproteinases has, in addition to the proteinase domain, a disintegrin-like domain and a cysteine-rich domain. Recent evidence has shown that the nonproteinase domains of the P-III class of hemorrhagic metalloproteinases function in the inhibition of platelet aggregation by blocking essen...

journal_title：Archives of biochemistry and biophysics

authors： Jia LG,Wang XM,Shannon JD,Bjarnason JB,Fox JW

更新日期：2000-01-01 00:00:00

abstract：:A solution hybridization assay employing specific synthetic oligodeoxynucleotide probes was developed to study the regulation of intestinal calbindin-D28K mRNA. The technique is rapid and quantitative and eliminates the need for sample transfer, blotting, autoradiography, and densitometry. Following validation of the ...

journal_title：Archives of biochemistry and biophysics

authors： Fullmer CS

更新日期：1990-11-15 00:00:00

abstract：:A cytoplasmic 10S ribonucleoprotein particle (iRNP), which is isolated from chick embryonic muscle, is a potent inhibitor of mRNA translation in vitro and contains a 4S translation inhibitory RNA species (iRNA). The iRNP particle shows similarity in size to the small nuclear ribonucleoprotein (snRNP) particles. Certai...

journal_title：Archives of biochemistry and biophysics

authors： Boak AM,Kovacs SA,Agris PF,Chakraborty D,Sarkar S

更新日期：1986-07-01 00:00:00

abstract：:2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD, dioxin) produces many of its biological effects by binding to a soluble, intracellular protein (the Ah receptor (AhR]. The hepatic AhR, from a variety of species, is present in low salt cytosol as a form which sediments at 8-10 S. High salt (0.4 M KCL) dissociates the rat, gu...

journal_title：Archives of biochemistry and biophysics

authors： Denison MS,Vella LM

更新日期：1990-03-01 00:00:00

abstract：:Cellular distribution in rat livers of the mannan-binding protein, a liver lectin specific for mannose and N-acetylglucosamine was investigated. Estimation of mannan binding activity of isolated cells demonstrated that more than 99% of the mannan binding activity recovered from whole livers was derived from hepatocyte...

journal_title：Archives of biochemistry and biophysics

authors： Mori K,Kawasaki T,Yamashina I

更新日期：1983-04-15 00:00:00

abstract：:Pretreatment of rabbits with imidazole resulted in a twofold increase in hepatic microsomal cytochrome P-450 content, with the apparent induction of two or more distinct forms of the cytochrome [K. K. Hajek and R. F. Novak (1982) Biochem. Biophys. Res. Commun. 108, 664-672]. The metabolic properties of imidazole-induc...

journal_title：Archives of biochemistry and biophysics

authors： Kaul KL,Novak RF

更新日期：1984-12-01 00:00:00

abstract：:Geranylgeranyl diphosphate synthase supplies the essential acyclic precursor for Taxol biosynthesis in methyl jasmonate-induced Taxus canadensis suspension cell cultures. A cDNA encoding this prenyltransferase was cloned from an induced T. canadensis cell library. The recombinant enzyme expressed in yeast was confirme...

journal_title：Archives of biochemistry and biophysics

authors： Hefner J,Ketchum RE,Croteau R

更新日期：1998-12-01 00:00:00

abstract：:Changes in the levels of various molecular species of N-acylethanolamine in CdCl2-administered rat testis were examined. We found that the levels of various N-acylethanolamines including anandamide (N-arachidonoylethanolamine), an endogenous cannabinoid receptor ligand, were dramatically increased in CdCl2-admin-ister...

journal_title：Archives of biochemistry and biophysics

authors： Kondo S,Sugiura T,Kodaka T,Kudo N,Waku K,Tokumura A

更新日期：1998-06-15 00:00:00

abstract：:The binding of Toxin A isolated from Clostridium difficile to rabbit erythrocyte glycolipids has been studied. Total lipid extracts from rabbit erythrocytes were subjected to thin-layer chromatography and toxin-binding glycolipids detected by using 125I-labeled Toxin A in a direct binding overlay technique. Two major ...

journal_title：Archives of biochemistry and biophysics

authors： Clark GF,Krivan HC,Wilkins TD,Smith DF

更新日期：1987-08-15 00:00:00

abstract：:Nanomechanical properties of living cells, as measured with atomic force microscopy (AFM), are increasingly recognized as criteria that differentiate normal and pathologically altered cells. Locally measured cell elastic properties, described by the parameter known as Young's modulus, are currently proposed as a new d...

journal_title：Archives of biochemistry and biophysics

authors： Pogoda K,Pięta E,Roman M,Piergies N,Liberda D,Wróbel TP,Janmey PA,Paluszkiewicz C,Kwiatek WM

更新日期：2021-01-15 00:00:00

abstract：:Protein synthesis and lipid peroxidation were evaluated in rat liver slices incubated in the presence of oxidants and protein synthesis inhibitors. Protein synthesis by rat liver slices was evaluated by [3H]leucine incorporation into the trichloroacetic acid (TCA)-insoluble material, and lipid peroxidation was evaluat...

journal_title：Archives of biochemistry and biophysics

authors： Fraga CG,Zamora R,Tappel AL

更新日期：1989-04-01 00:00:00

abstract：:The activity of acetyl-CoA carboxylase (ACC), the rate-limiting enzyme of fatty acid biosynthesis, can be regulated by both adenine and guanine nucleotides in vitro. We have employed two inhibitors of IMP dehydrogenase, ribavarin and tiazofurin, to investigate a possible role for intracellular nucleotides in ACC regul...

journal_title：Archives of biochemistry and biophysics

authors： Witters LA,Mendel DB,Colliton JW

更新日期：1987-01-01 00:00:00

abstract：:Quantitative estimation of cytosolic Ca2+- and phospholipid-dependent protein kinase (PKC) activity was performed by polyacrylamide gel electrophoresis under nondenaturating conditions (PAGE). With this method less than 50 micrograms of cytosol protein can be accurately quantitated for PKC activity. The amount of cyto...

journal_title：Archives of biochemistry and biophysics

authors： Fabbro D,Jungmann RA,Eppenberger U

更新日期：1985-05-15 00:00:00

abstract：:Phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, stimulates both the cellular uptake of radiolabeled choline and the activity of CTP:phosphocholine cytidylyltransferase resulting in increased incorporation of choline into phosphatidylcholine (PtdCho). Because of these multiple actions, it...

journal_title：Archives of biochemistry and biophysics

authors： Kiss Z

更新日期：1996-11-01 00:00:00

abstract：:The protein Mop from Haemophilus influenzae is a member of the molbindin family of proteins. Using isothermal titration calorimetry (ITC), Mop was observed to bind molybdate at two distinct sites with a stoichiometry of 8 mol molybdate per Mop hexamer. Six moles of molybdate bound endothermically at high affinity site...

journal_title：Archives of biochemistry and biophysics

authors： Masters SL,Howlett GJ,Pau RN

更新日期：2005-07-01 00:00:00

abstract：:Fresh human A1 erythrocytes, washed and pretreated in phosphate buffer with or without papain, were incubated at 37 degrees C with blood group-degrading enzymes from the human fecal Ruminococcus torques strain IX-70. The effects were assayed as changes in hemagglutination patterns, and blood group activities of alkali...

journal_title：Archives of biochemistry and biophysics

authors： Falk P,Hoskins LC,Lindstedt R,Svanborg C,Larson G

更新日期：1991-11-01 00:00:00