Unified analysis of optical absorption spectra of carotenoids based on a stochastic model.

abstract：:No major structural alteration of alkaline phosphatase can be observed in the early stages of enzyme oxidative inactivation by the ascorbate model system. Fluorescence changes of protein-bound 8-anilino-1-naphthalenesulfonic acid suggest, however, that localized modifications take place. Oxidized alkaline phosphatase ...

journal_title：Archives of biochemistry and biophysics

authors： Mordente A,Martorana GE,Miggiano GA,Meucci E,Santini SA,Castelli A

更新日期：1988-08-01 00:00:00

abstract：:Interactions of six carbohydrates (trehalose, sucrose, glucose, raffinose, inositol, and glycerol) with dry dipalmitoylphosphatidylcholine (DPPC) were studied using differential scanning calorimetry (DSC) and infrared spectroscopy (ir) in order to elucidate the mechanism by which some of these carbohydrates preserve s...

journal_title：Archives of biochemistry and biophysics

authors： Crowe LM,Crowe JH,Chapman D

更新日期：1985-01-01 00:00:00

abstract：:Epidemiological evidence strongly suggests that lycopene consumption contributes to prostate cancer risk reduction. Preclinical studies show that lycopene acts via different mechanisms, which have the potential to cooperate in reducing the proliferation of normal and cancerous prostate epithelial cells, in reducing DN...

journal_title：Archives of biochemistry and biophysics

authors： Wertz K,Siler U,Goralczyk R

更新日期：2004-10-01 00:00:00

abstract：:We have characterized a Brassica rapa mutant (WR586) that has low levels of polyunsaturated fatty acids (D.L. Auld et al., 1992, Crop Sci. 32, 657-662). The mutant lacked oleoyl-phosphatidylcholine desaturase (ODS) activity when assayed in 6-day-old seedlings. To further characterize the mutant, the leaf fatty acid co...

journal_title：Archives of biochemistry and biophysics

authors： Lee MS,Guerra DJ

更新日期：1994-11-15 00:00:00

abstract：:The oxidation of quercetin by horseradish peroxidase/H(2)O(2) was studied in the absence but especially also in the presence of glutathione (GSH). HPLC analysis of the reaction products formed in the absence of GSH revealed formation of at least 20 different products, a result in line with other studies reporting the ...

journal_title：Archives of biochemistry and biophysics

authors： Awad HM,Boersma MG,Vervoort J,Rietjens IM

更新日期：2000-06-15 00:00:00

abstract：:Peroxynitrite crosses the red blood cell (RBC) membrane and reacts with hemoglobin (Hb) producing mainly metHb, which is reduced back to ferrousHb by NADH- and NADPH-dependent reductases. Peroxynitrite also induces band 3 (B3) tyrosine phosphorylation, a signaling pathway believed to activate glucose metabolism. This ...

journal_title：Archives of biochemistry and biophysics

authors： Metere A,Iorio E,Pietraforte D,Podo F,Minetti M

更新日期：2009-04-15 00:00:00

abstract：:This paper presents previously unobserved signals in the 1H NMR spectra of oxidized and reduced [2Fe-2S]-ferredoxin from Anabaena 7120 detected at 400, 500, and 600 MHz. The signals shifted to low field exhibited longitudinal relaxation (T1) values in the range of 100-400 microseconds and line widths in the range of 1...

journal_title：Archives of biochemistry and biophysics

authors： Skjeldal L,Westler WM,Markley JL

更新日期：1990-05-01 00:00:00

abstract：:A number of early experiments suggested a relationship between methyl group metabolism and the exocrine secretion of the pancreas. These included nutritional studies showing that ethionine, the ethyl analog of methionine which inhibits cellular methylation reactions, is a specific pancreatic toxin. Other studies indic...

journal_title：Archives of biochemistry and biophysics

authors： Capdevila A,Decha-Umphai W,Song KH,Borchardt RT,Wagner C

更新日期：1997-09-01 00:00:00

abstract：:gG.2 glycoprotein was purified by H966 monoclonal antibodies linked to Sepharose from herpes simplex virus type 2-infected HEp-2 cells labeled with [3H] glucosamine. The glycoprotein was subjected to Pronase digestion and the glycopeptides were fractionated by Con A-Sepharose in a major fraction (88.5% of total radioa...

journal_title：Archives of biochemistry and biophysics

authors： Serafini-Cessi F,Malagolini N,Dall'Olio F,Pereira L,Campadelli-Fiume G

更新日期：1985-08-01 00:00:00

abstract：:Cultured arterial smooth muscle cells have been found to contain an activatable neutral cholesteryl esterase (EC 3.1.1.13). This enzyme is similar to that previously described in adipose tissue, adrenal cortex, and aortic homogenates. Although both the lysosomal (acid) and cytoplasmic (neutral) cholesteryl esterases w...

journal_title：Archives of biochemistry and biophysics

authors： Hajjar DP

更新日期：1986-05-15 00:00:00

abstract：:Production of hydroxyl radicals (HO.) by substrate-supplemented beef heart submitochondrial particles was studied by electron paramagnetic resonance in conjunction with the spin trap 5,5'-dimethyl-1-pirroline-N-oxide (DMPO). Supplementation of submitochondrial particles with NADH or succinate in the presence of antimy...

journal_title：Archives of biochemistry and biophysics

authors： Giulivi C,Boveris A,Cadenas E

更新日期：1995-02-01 00:00:00

abstract：:Aryl hydrocarbon hydroxylase activity is most closely associated with cytochrome P-450c in the rat and cytochrome P1-450 in the mouse. The sequence for the orthologous human gene coding for this enzymatic activity has been determined from several sources: cytochrome P-450c isolated from human embryonic DNA [K. Kawajir...

journal_title：Archives of biochemistry and biophysics

authors： Iversen PL,Heiger WJ,Bresnick E,Hines RN

更新日期：1987-07-01 00:00:00

abstract：:The ionophore properties of cationomycin and monensin were studied on human erythrocytes by measuring Na+ influx by 23Na NMR and concomitant K+ efflux by potentiometry in the presence of increasing amounts of serum. Both ion currents (Na+ or K+) decreased linearly with the reciprocal of serum amount. The serum effects...

journal_title：Archives of biochemistry and biophysics

authors： Gibot S,Jeminet G,Juillard J,Gumila C,Ancelin ML,Vial H,Delort AM

更新日期：1999-03-15 00:00:00

abstract：:Three iodinated derivatives of ubiquitin have been synthesized and these derivatives have been characterized in the ubiquitin-dependent protein degradation system. With chloramine-T as the oxidant, a derivative containing monoiodotyrosine is formed in the presence of 1 M KI and a derivative containing diiodotyrosine i...

journal_title：Archives of biochemistry and biophysics

authors： Cox MJ,Haas AL,Wilkinson KD

更新日期：1986-11-01 00:00:00

abstract：:The glutamylation of methotrexate has been evaluated in H35 hepatoma cells in vitro as a function of the conditions of culture. Glutamylation yields methotrexate polyglutamate with two to five additional glutamate residues and is a saturable process. The rate of glutamylation increases little above 10 microM extracell...

journal_title：Archives of biochemistry and biophysics

authors： Nimec Z,Galivan J

更新日期：1983-10-15 00:00:00

abstract：:Glycerol is a dietary component which is metabolized primarily by the liver and kidney where it is used mainly for glucose synthesis. The metabolism of glycerol is very similar to that of dihydroxyacetone which can be considered its more oxidized counterpart. The effects of these substrates on hepatic lipogenesis and ...

journal_title：Archives of biochemistry and biophysics

authors： Carmona A,Freedland RA

更新日期：1989-05-15 00:00:00

abstract：:A procedure was developed for the purification of an acetone-inducible form of cytochrome P-450 (P-450ac) to electrophoretical homogeneity from liver microsomes of acetone-treated rats. The P-450ac preparation containing 16.0 to 16.5 nmol P-450/mg protein moved as a single protein band with an estimated molecular weig...

journal_title：Archives of biochemistry and biophysics

authors： Patten CJ,Ning SM,Lu AY,Yang CS

更新日期：1986-12-01 00:00:00

abstract：:All six available lysine residues in bacteriorhodopsin were amidinated with dimethyl-3,3'-dithiobispropionimidate, which is a crosslinking agent. The photocycle was studied by measuring light absorption and electric signals. The data show an essential change in the photocycle: instead of single components, the rise of...

journal_title：Archives of biochemistry and biophysics

authors： Tóth-Boconádi R,Taneva SG,Kiselev AV,Abdulaev NG,Keszthelyi L

更新日期：1988-02-01 00:00:00

abstract：:A variety of experimental conditions elicit increases in mitochondrial permeability that can be differentiated from the classic cyclosporin A (CsA)-sensitive mitochondrial permeability transition (MPT). For example, butylated hydroxytoluene, signal peptides, and the hormone thyroxine have been shown to promote increas...

journal_title：Archives of biochemistry and biophysics

authors： Sultan A,Sokolove PM

更新日期：2001-02-01 00:00:00

abstract：:Two clones with homology to the alpha-hydroxynitrile lyase (HNL) cDNA clone, MeHNL10, were isolatedfrom a lambdaEMBL3 cassava (Manihot esculenta Crantz) genomic library. Analysis of the sequences showed that both genomic clones contain HNL genes (MeHNL4, MeHNL24) which are interrupted by two introns. RT-PCR analysis o...

journal_title：Archives of biochemistry and biophysics

authors： Hughes J,Keresztessy Z,Brown K,Suhandono S,Hughes MA

更新日期：1998-08-15 00:00:00

abstract：:Glucosamine-6-phosphate deaminase (EC 5.3.1.10) from dog kidney cortex was purified to homogeneity, as judged by several criteria of purity. The purification procedure was based on two biospecific affinity chromatography steps, one of them using N-epsilon-amino-n-hexanoyl-D-glucosamine-6-phosphate agarose, an immobili...

journal_title：Archives of biochemistry and biophysics

authors： Lara-Lemus R,Libreros-Minotta CA,Altamirano MM,Calcagno ML

更新日期：1992-09-01 00:00:00

abstract：:Studies in vascular smooth muscle cells suggest that, angiotensin II (Ang II)-mediated cellular response requires transactivation of epidermal growth factor receptor (EGF-R), and involves tyrosine phosphorylation of caveolin-1. Here we demonstrate that, exposure of WB rat liver cells to Ang II does not cause transacti...

journal_title：Archives of biochemistry and biophysics

authors： Krishna SB,Alfonso LF,Thekkumkara TJ,Abbruscato TJ,Bhat GJ

更新日期：2007-01-01 00:00:00

abstract：:Intracellular glutathione (GSH) depletion induced by buthionine sulfoximine (BSO) caused cell death that seemed to be apoptosis in C6 rat glioma cells. Arachidonic acid (AA) promoted BSO-induced cell death by accumulating reactive oxygen species (ROS) or hydroperoxides. AA inhibited caspase-3 activation and internucle...

journal_title：Archives of biochemistry and biophysics

authors： Higuchi Y,Yoshimoto T

更新日期：2002-04-01 00:00:00

abstract：:Uteroglobin has been purified from hare lung by gel filtration and chromatography on carboxymethyl-cellulose. Hare uteroglobin appears homogeneous by electrophoresis under both denaturing and nondenaturing conditions. Its chemical and immunological properties as well as its ability to bind progesterone are compared to...

journal_title：Archives of biochemistry and biophysics

authors： Soledad López de Haro M,Nieto A

更新日期：1983-10-15 00:00:00

abstract：:The major phenobarbital-inducible form of cytochrome P-450 (cytochrome P-450 PB) was purified to homogeneity from rat liver microsomes and rabbit antibodies prepared against the purified enzyme. Using these antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed for the detection of cytochrome P-450 PB ...

journal_title：Archives of biochemistry and biophysics

authors： Seidel SL,Shawver LK,Shires TK

更新日期：1984-03-01 00:00:00

abstract：:Stationary phase inocula were more susceptible to the growth inhibitory effect of paraquat than were log phase inocula and this difference was exacerbated in strains overproducing superoxide dismutases (SOD). Glucose-6-phosphate dehydrogenase (G-6-PD), a member of the soxR regulon, was induced by paraquat promptly in ...

journal_title：Archives of biochemistry and biophysics

authors： Liochev SI,Fridovich I

更新日期：1992-04-01 00:00:00

abstract：:A kinetic study of acetaminophen oxidation by tyrosinase in the presence of a physiological nucleophilic agent such as the amino acid L-proline is performed in the present paper. The o-quinone product of the catalytic activity, 4-acetamido-o-benzoquinone, becomes unstable through the chemical addition of L-proline, in...

journal_title：Archives of biochemistry and biophysics

authors： Valero E,Varón R,García-Carmona F

更新日期：2003-08-15 00:00:00

abstract：:Thyroid peroxidase (TPO) simultaneously catalyzes two very different types of reaction in the thyroid gland- iodination and coupling. The present study addresses the mechanism of this simultaneous dual activity. Compound I, the two-electron oxidation product of TPO, exists in two different forms--an oxoferryl porphyri...

journal_title：Archives of biochemistry and biophysics

authors： Taurog A,Dorris ML,Doerge DR

更新日期：1996-06-01 00:00:00

abstract：:The amino acid L-lysine is synthesized in Saccharomyces cerevisiae via the α-aminoadipate pathway. An as yet unidentified PLP-containing aminotransferase is thought to catalyze the formation of α-aminoadipate from α-ketoadipate in the L-lysine biosynthetic pathway that could be the yeast Aro8 gene product. A screen of...

journal_title：Archives of biochemistry and biophysics

authors： Karsten WE,Reyes ZL,Bobyk KD,Cook PF,Chooback L

更新日期：2011-12-01 00:00:00

abstract：:Oxidative treatments of wood pulp lignin by one-electron-abstracting enzymatic or chemical systems result in modification phenomena which are not fully described in terms of those known from lignin model compound studies. The generation of, e.g., long-lived radicals necessitates nondestructive spectroscopic analysis o...

journal_title：Archives of biochemistry and biophysics

authors： Barsberg S

更新日期：2002-08-01 00:00:00