Abstract:
:Oxidative treatments of wood pulp lignin by one-electron-abstracting enzymatic or chemical systems result in modification phenomena which are not fully described in terms of those known from lignin model compound studies. The generation of, e.g., long-lived radicals necessitates nondestructive spectroscopic analysis of the lignin polymer for a proper characterization of these. The present work exposes a complexity of spectroscopic modification phenomena, which has not previously been realized. This is achieved by a laccase-mediator system, where the mediator is an aromatic low-molecular-weight compound, which mediates the electron abstraction between the lignin and the enzyme laccase. It is demonstrated that the modification generated exhibits qualitatively different temporal phases. The mechanisms are partly explained in terms of Marcus electron transfer theory, and it is suggested that these may play a role in the in vivo synthesis and degradation of lignin.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Barsberg Sdoi
10.1016/s0003-9861(02)00225-4subject
Has Abstractpub_date
2002-08-01 00:00:00pages
62-70issue
1eissn
0003-9861issn
1096-0384pii
S0003986102002254journal_volume
404pub_type
杂志文章abstract::Salicylhydroxamic acid reductase, which catalyzes the reduction of salicylhydroxamic acid to salicylamide, was purified from rat liver cytosol. The purification procedure consisted of fractionation with ammonium sulfate, chromatography with Phenyl-Toyopearl 650, DEAE-cellulose, hydroxyapatite, and Sephadex G-200, and ...
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pub_type: 杂志文章
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更新日期:2018-09-15 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
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journal_title:Archives of biochemistry and biophysics
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journal_title:Archives of biochemistry and biophysics
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journal_title:Archives of biochemistry and biophysics
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
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