当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Metabolic impact of overexpression of liver glycogen synthase with serine-to-alanine substitutions in rat primary hepatocytes.

Metabolic impact of overexpression of liver glycogen synthase with serine-to-alanine substitutions in rat primary hepatocytes.

Abstract：

:To investigate the effect of elevation of liver glycogen synthase (GYS2) activity on glucose and glycogen metabolism, we performed adenoviral overexpression of the mutant GYS2 with six serine-to-alanine substitutions in rat primary hepatocytes. Cell-free assays demonstrated that the serine-to-alanine substitutions caused constitutive activity and electrophoretic mobility shift. In rat primary hepatocytes, overexpression of the mutant GYS2 significantly reduced glucose production by 40% and dramatically induced glycogen synthesis via the indirect pathway rather than the direct pathway. Thus, we conclude that elevation of glycogen synthase activity has an inhibitory effect on glucose production in hepatocytes by shunting gluconeogenic precursors into glycogen. In addition, although intracellular compartmentation of glucose-6-phosphate (G6P) remains unclear in hepatocytes, our results imply that there are at least two G6P pools via gluconeogenesis and due to glucose phosphorylation, and that G6P via gluconeogenesis is preferentially used for glycogen synthesis in hepatocytes.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Kadotani A,Fujimura M,Nakamura T,Ohyama S,Harada N,Maruki H,Tamai Y,Kanatani A,Eiki J,Nagata Y

doi

10.1016/j.abb.2007.08.006

subject

Has Abstract

pub_date

2007-10-15 00:00:00

pages

283-9

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(07)00398-0

journal_volume

466

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Characterization of the peribacteroid membrane ATPase of lupin root nodules.

    abstract：:Peribacteroid membranes can be isolated in essentially pure form from 20-day lupin root nodules by osmotic shock of the purified membrane enclosed bacteroids. The ATPase (EC 3.6.1.3) associated with this membrane has an acid pH optimum (5.25) and is specific for ATP (Mg-ATP Km = 0.16 mM). The enzyme activity requires ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90322-0

    authors： Domigan NM,Farnden KJ,Robertson JG,Monk BC

    更新日期：1988-08-01 00:00:00

  • Changes of energy metabolism induced by 1-methyl-4-phenylpyridinium (MPP+)-related compounds in rat pheochromocytoma PC12 cells.

    abstract：:We examined effects of three structurally related pyridinium compounds, 1-methyl-4-phenylpyridinium (MPP+), paraquat, and 1-methyl-4-(4'-nitrophenyl) pyridinium (analog 1), on the energy metabolism in pheochromocytoma PC12 cells. MPP+ inhibited the intracellular NADH oxidation by the mitochondrial respiratory chain, j...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.9727

    authors： Kang D,Miyako K,Kuribayashi F,Hasegawa E,Mitsumoto A,Nagano T,Takeshige K

    更新日期：1997-01-01 00:00:00

  • Papillomavirus binding factor (PBF)-mediated inhibition of cell growth is regulated by 14-3-3beta.

    abstract：:The cellular factor, papillomavirus (PV)-binding factor (PBF)/Huntington's disease binding protein 2 (HDBP2), was identified by its ability to bind regulatory sequences of certain papillomavirus types as well as the Huntington's disease gene. PBF is thought to be a novel nuclear-shuttling transcription factor with unk...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.04.017

    authors： Sichtig N,Silling S,Steger G

    更新日期：2007-08-01 00:00:00

  • Effect of age and dietary calcium on intestinal calbindin D-9k expression in the rat.

    abstract：:The capacity of rats and humans to adapt to low dietary Ca by increasing intestinal Ca absorption declines with age. The intestinal calbindin-D-9k protein (calbindin) is thought to play a role in the transcellular transport of Ca across the mammalian intestine. The purpose of these studies was to determine the effect ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2003.09.025

    authors： Armbrecht HJ,Boltz MA,Bruns ME

    更新日期：2003-12-01 00:00:00

  • The oxidizing power of the glutathione thiyl radical as measured by its electrode potential at physiological pH.

    abstract：:The oxidizing power of the thiyl radical (GS*) produced on oxidation of glutathione (GSH) was determined as the mid-point electrode potential (reduction potential) of the one-electron couple E(m)(GS*,H+/GSH) in water, as a function of pH over the physiological range. The method involved measuring the equilibrium const...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.03.002

    authors： Madej E,Wardman P

    更新日期：2007-06-01 00:00:00

  • Raman spectroscopic evidence for a disulfide bridge in calf gamma II crystallin.

    abstract：:Laser Raman spectroscopy has been applied to native and dithiothreitol-treated bovine cortical gamma II crystallin to examine the state of the thiol groups and the presence of a putative disulfide bridge. The data reveal significant differences in two key spectral regions. In the thiol stretching region (2500-2600 cm-...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90106-9

    authors： Pande J,McDermott MJ,Callender RH,Spector A

    更新日期：1989-02-15 00:00:00

  • Functional significance of C-terminal mobile domain of cardiac troponin I.

    abstract：:Ca2+-regulation of cardiac contractility is mediated through the troponin complex, which comprises three subunits: cTnC, cTnI, and cTnT. As intracellular [Ca2+] increases, cTnI reduces its binding interactions with actin to primarily interact with cTnC, thereby enabling contraction. A portion of this regulatory switch...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2017.09.017

    authors： Bohlooli Ghashghaee N,Tanner BCW,Dong WJ

    更新日期：2017-11-15 00:00:00

  • Triton X-100 or octyl glucoside inactivates acyl-CoA:cholesterol acyltransferase 1 by dissociating it from a two-fold dimer to a two-fold monomer.

    abstract：:Cholesterol is an important lipid molecule and is needed for all mammalian cells. In various cell types, excess cholesterol is stored as cholesteryl esters; acyl-CoA:cholesterol acyltransferase 1 (ACAT1) plays an essential role in this storage process. ACAT1 is located at the endoplasmic reticulum and has nine transme...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2019.06.006

    authors： Neumann B,Chang CCY,Chang TY

    更新日期：2019-08-15 00:00:00

  • MicroRNA-27a regulates the proliferation, chemosensitivity and invasion of human ovarian cancer cell lines by targeting Cullin 5.

    abstract：:Reports suggest that microRNAs have implications in the development of several diseases including cancer. It is therefore believed that miRs may act as therapeutic targets for cancer treatment. The treatment of ovarian cancer is mainly obstructed by lack of biomarkers and efficient drug targets. Against this backdrop,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2019.04.009

    authors： Si L,Jia Y,Lin R,Jian W,Yu Q,Yang S

    更新日期：2019-06-15 00:00:00

  • Modification of hepatic vitamin E stores in vivo. II. Alterations in plasma and liver vitamin E content by 1,2-dibromoethane.

    abstract：:Previous studies with methyl ethyl ketone peroxide (MEKP), a radical generator, showed depletion of plasma vitamin E and liver glutathione (GSH) levels prior to a decrease of liver vitamin E levels. Since hepatic pools of this vitamin may serve to maintain circulating levels of vitamin E under conditions of oxidative ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90218-8

    authors： Warren DL,Brown MK,Reed DJ

    更新日期：1991-08-01 00:00:00

  • NahR: effects of replacements at Asn 169 and Arg 248 on promoter binding and inducer recognition.

    abstract：:NahR, a member of the LysR regulator family, is a positive transcriptional regulator for genes of the naphthalene degradation pathway in Pseudomonas sp. To study NahR binding properties, five single and six double mutants were made at residues 169 and/or 248, which are located in the central inducer recognition domain...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2004.10.020

    authors： Park HH,Lee HY,Lim WK,Shin HJ

    更新日期：2005-02-01 00:00:00

  • Both PA63 and PA83 are endocytosed within an anthrax protective antigen mixed heptamer: a putative mechanism to overcome a furin deficiency.

    abstract：:Anthrax toxin consists of protective antigen (PA), and lethal (LF) and edema (EF) factors. A 83 kDa PA monomer (PA83) precursor binds to the cell receptor. Furin-like proprotein convertases (PCs) cleave PA83 to generate cell-bound 63 kDa protein (PA63). PA63 oligomerizes to form a ring-shaped heptamer that binds LF-EF...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2005.11.013

    authors： Chekanov AV,Remacle AG,Golubkov VS,Akatov VS,Sikora S,Savinov AY,Fugere M,Day R,Rozanov DV,Strongin AY

    更新日期：2006-02-01 00:00:00

  • Mangiferin, a natural occurring glucosyl xanthone, increases susceptibility of rat liver mitochondria to calcium-induced permeability transition.

    abstract：:Mitochondrial permeability transition (MPT) is a Ca(2+)-dependent, cyclosporine A-sensitive, non-selective inner membrane permeabilization induced by a wide range of agents or conditions, which has often been associated with necrotic or apoptotic cell death. When mitochondria isolated from livers of rats treated with ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2005.05.015

    authors： Andreu GL,Delgado R,Velho JA,Curti C,Vercesi AE

    更新日期：2005-07-15 00:00:00

  • Binding of soluble myelin basic protein to various conformational forms of alpha2-macroglobulin.

    abstract：:Myelin basic protein is known to be released into the circulation following traumatic injuries or demyelination within the central nervous system, resulting in the generation of potentially immunogenic myelin basic protein material. In this investigation we have studied the binding of bovine and human myelin basic pro...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1998.0902

    authors： Gunnarsson M,Jensen PE

    更新日期：1998-11-15 00:00:00

  • The ATP4- receptor-operated ion channel of human lymphocytes: inhibition of ion fluxes by amiloride analogs and by extracellular sodium ions.

    abstract：:Extracellular ATP is known to increase the membrane permeability of a variety of cells. Addition of ATP to human leukemic lymphocytes loaded with the Ca2+ indicator, fura-2, induced a rise in cytosolic Ca2+ concentration which was attenuated or absent in NaCl media compared with KCl, choline Cl, or NMG Cl media. In co...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90010-t

    authors： Wiley JS,Chen R,Wiley MJ,Jamieson GP

    更新日期：1992-02-01 00:00:00

  • Biochemical and functional insights on the triheme cytochrome PpcA from Geobacter metallireducens.

    abstract：:G. metallireducens bacterium has highly versatile respiratory pathways that provide the microorganism an enormous potential for many biotechnological applications. However, little is known about the structural and functional properties of its electron transfer components. In this work, the periplasmic cytochrome PpcA ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2018.02.017

    authors： Portela PC,Fernandes TM,Dantas JM,Ferreira MR,Salgueiro CA

    更新日期：2018-04-15 00:00:00

  • Further characterization of allergenically active oligosaccharitols isolated from a sea squirt H-antigen.

    abstract：:Complete primary structures of five allergenically active oligosaccharitols (HPG-beta 2-N5a, -N6, -N7a, -N7b, and -N9) derived from a sea squirt H-antigen were studied. Structural characterization was carried out by a new method in which products of limited periodate oxidation, followed by derivatization with p-aminob...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90569-5

    authors： Ohta M,Matsuura F,Kobayashi Y,Shigeta S,Ono K,Oka S

    更新日期：1991-11-01 00:00:00

  • Cardiotonic actions of quercetin and its metabolite tamarixetin through a digitalis-like enhancement of Ca2+ transients.

    abstract：:The plant-derived flavonoid, quercetin (QCT), has many biological actions, including cardioprotective actions, resulting from its antioxidant and anti-inflammatory effects. In this study, effects of QCT and its metabolites on the contraction and Ca2+ transients (CaT) of mouse single cardiomyocytes were simultaneously ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2017.11.009

    authors： Hayamizu K,Morimoto S,Nonaka M,Hoka S,Sasaguri T

    更新日期：2018-01-01 00:00:00

  • New analogs of 2-methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 with conformationally restricted side chains: evaluation of biological activity and structural determination of VDR-bound conformations.

    abstract：:We have successfully prepared E- and Z- isomers of 17-20 dehydro analogs of 2-methylene-19-nor-(20S)-1alpha,25-dihydroxyvitamin D3 (2MD). Both isomers bind to the recombinant rat vitamin D receptor (VDR) with high affinity. The Z-isomer (Vit-III 17-20Z) displays activity in vivo and in vitro that is similar to 2MD. Th...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2006.11.029

    authors： Vanhooke JL,Tadi BP,Benning MM,Plum LA,DeLuca HF

    更新日期：2007-04-15 00:00:00

  • A novel variant of mouse MATE-1 H+/organic cation antiporter with a long hydrophobic tail.

    abstract：:Mammalian multidrug and toxic compound extrusion 1 (MATE1) are polyspecific H+-coupled exporters of organic cations (OCs) and responsible for excretion of metabolic waste products and xenobiotics. Here, we report a novel variant of mouse MATE1, mMATE1b, that has a long carboxyl terminal hydrophobic tail homologous to ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.10.010

    authors： Kobara A,Hiasa M,Matsumoto T,Otsuka M,Omote H,Moriyama Y

    更新日期：2008-01-15 00:00:00

  • Identification of the FTBL protein of Sumner and Dounce as a leucine aminopeptidase.

    abstract：:The crystalline beef liver protein of Sumner and Dounce (A. L. Dounce, P. Z. Allen, and G. A. Mourtzikos (1978) Arch. Biochem. Biophys. 188, 251-265) termed FTBL (football) protein because of the shape of its crystals, has been identified as a crystalline leucine aminopeptidase (LAP), on the basis of its high specific...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90537-6

    authors： Dounce AL,Allen PZ

    更新日期：1987-08-15 00:00:00

  • Ethanol exposure decreases mitochondrial outer membrane permeability in cultured rat hepatocytes.

    abstract：:Mitochondrial metabolism depends on movement of hydrophilic metabolites through the mitochondrial outer membrane via the voltage-dependent anion channel (VDAC). Here we assessed VDAC permeability of intracellular mitochondria in cultured hepatocytes after plasma membrane permeabilization with 8 microM digitonin. Block...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.10.036

    authors： Holmuhamedov E,Lemasters JJ

    更新日期：2009-01-15 00:00:00

  • Metabolism of monoepoxides of methyl linoleate: bioactivation and detoxification.

    abstract：:Leukotoxin (ltx) and isoleukotoxin (iltx) methyl esters, are metabolites of methyl linoleic acid, an essential fatty acid. They have been associated with acute respiratory distress syndrome. The observed toxicity of ltx and iltx is, in fact, due to the metabolism of the epoxides to their corresponding diols by soluble...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.1753

    authors： Greene JF,Williamson KC,Newman JW,Morisseau C,Hammock BD

    更新日期：2000-04-15 00:00:00

  • Cleavage of vimentin by different retroviral proteases.

    abstract：:Proteases (PRs) of retroviruses cleave viral polyproteins into their mature structural proteins and replication enzymes. Besides this essential role in the replication cycle of retroviruses, PRs also cleave a variety of host cell proteins. We have analyzed the in vitro cleavage of mouse vimentin by proteases of human ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.1776

    authors： Snásel J,Shoeman R,Horejsí M,Hrusková-Heidingsfeldová O,Sedlácek J,Ruml T,Pichová I

    更新日期：2000-05-15 00:00:00

  • Resonance Raman spectroscopic evaluation of skin carotenoids as a biomarker of carotenoid status for human studies.

    abstract：:Resonance Raman spectroscopy (RRS) is a non-invasive method that has been developed to assess carotenoid status in human tissues including human skin in vivo. Skin carotenoid status has been suggested as a promising biomarker for human studies. This manuscript describes research done relevant to the development of thi...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2013.06.007

    authors： Mayne ST,Cartmel B,Scarmo S,Jahns L,Ermakov IV,Gellermann W

    更新日期：2013-11-15 00:00:00

  • Host cell signal transduction during Listeria monocytogenes infection.

    abstract：:The facultative intracellular bacterial pathogen Listeria monocytogenes invades and multiplies in many mammalian cell types. During the interaction with its host cells it strongly interferes with and modulates host cell functions. In the present review we summarize the current knowledge on the modulation of signal tra...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1006/abbi.1999.1487

    authors： Kuhn M,Pfeuffer T,Greiffenberg L,Goebel W

    更新日期：1999-12-01 00:00:00

  • Tobacco proliferating cell nuclear antigen binds directly and stimulates both activity and processivity of ddNTP-sensitive mungbean DNA polymerase.

    abstract：:PCNA is well known as a component of DNA replication system and plays important roles in multiple cellular pathways in addition to replication and repair. In this work we have demonstrated the physical and functional interaction between tobacco PCNA and mungbean ddNTP-sensitive DNA polymerase which shares many physico...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.09.009

    authors： Roy S,Roy Choudhury S,Mukherjee SK,Sengupta DN

    更新日期：2007-12-01 00:00:00

  • Identification of the active-site serine in human lecithin: cholesterol acyltransferase.

    abstract：:Purified human lecithin:cholesterol acyltransferase (LCAT) was covalently labeled by [3H]diisopropylflourophosphate with concomitant loss of enzymatic activity (M. Jauhiainen and P.J. Dolphin (1986) J. Biol. Chem. 261, 7023-7043). Some 60% of the enzyme was labeled in 1 h. Cyanogen bromide (CNBr) cleavage of the label...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90348-7

    authors： Farooqui JZ,Wohl RC,Kézdy FJ,Scanu AM

    更新日期：1988-03-01 00:00:00

  • Activation of Glut1 glucose transporter in response to inhibition of oxidative phosphorylation.

    abstract：:We have previously shown that exposure of Clone 9 cells to hypoxia, cyanide, or azide results in an acute stimulation of glucose transport that is largely mediated by "activation" of glucose transporter (Glut1) sites preexisting in the plasma membrane. However, it is not known whether inhibition of oxidative phosphory...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1320

    authors： Hamrahian AH,Zhang JZ,Elkhairi FS,Prasad R,Ismail-Beigi F

    更新日期：1999-08-15 00:00:00

  • Fluorescence properties of melanins from opioid peptides.

    abstract：:Recently our group synthesized a new class of melanins obtained by the tyrosinase-catalyzed oxidation of opioid peptides (opiomelanins). Owing to the presence of the peptide moiety such pigments exhibit high solubility in hydrophilic solvents, which allows spectroscopic investigations. In particular, the absence of so...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1403

    authors： Mosca L,De Marco C,Fontana M,Rosei MA

    更新日期：1999-11-01 00:00:00