Binding of soluble myelin basic protein to various conformational forms of alpha2-macroglobulin.

abstract：:The stability of glutathione peroxidase was assessed in vitro via oxidative inactivation by peroxides and a peroxidizing fatty acid and by renaturation and proteolysis. The stability of glutathione peroxidase to methyl ethyl ketone peroxide, H2O2, linoleic acid hydroperoxide, and peroxidizing methyl linolenate was com...

journal_title：Archives of biochemistry and biophysics

authors： Condell RA,Tappel AL

更新日期：1983-06-01 00:00:00

abstract：:Representatives of four different classes of isoquinoline alkaloids (aporphines, benzylisoquinolines, tetrahydroisoquinolines, and phthalideisoquinolines) inhibit glutamate dehydrogenase activity in both reaction directions. Representatives of five other groups of isoquinoline alkaloids have been without any significa...

journal_title：Archives of biochemistry and biophysics

authors： Kovár J

更新日期：1983-02-15 00:00:00

abstract：:From our topological arrangement model of prostaglandin I(2) synthase (PGIS) created by homology modeling and topology studies, we hypothesized that the helix F/G loop of PGIS contains a membrane contact region distinct from the N-terminal membrane anchor domain. To provide direct experimental data we have explored th...

journal_title：Archives of biochemistry and biophysics

authors： Wu J,So SP,Ruan KH

更新日期：2003-03-01 00:00:00

abstract：:The effect of a protein matrix on the processing of glycoprotein glycans by Golgi enzymes from plant seedlings has been determined with an artificial glycoprotein system, comparing the processing rates of glycan-(biotinyl)Asn (or glycan-(biotinamidohexanoyl)Asn) substrates either free or bound to avidin. An analysis o...

journal_title：Archives of biochemistry and biophysics

authors： Lu Y,Wold F

更新日期：1991-04-01 00:00:00

abstract：:Much effort has focused on the identification of MAPK cascades that are activated by the MEKK family of protein kinases. However, direct phosphorylation and regulation of the MEKK proteins has not been shown. To address this question, we have expressed recombinant (His)6FLAG.MEKK3 in Sf9 insect cells and tethered the ...

journal_title：Archives of biochemistry and biophysics

authors： Adams DG,Sachs NA,Vaillancourt RR

更新日期：2002-11-01 00:00:00

abstract：:Synthetic peptides corresponding to the phosphorylation site in the myosin regulatory light chain from smooth muscle, Lys-Lys-Arg-Ala-Arg-Ala-Thr-Ser-Asn-Val-Phe-Ala ([Ala14,15]MLC(11-23] and containing a variety of hydroxyamino acid analogs at position 19, were tested as substrates for the smooth muscle myosin light ...

journal_title：Archives of biochemistry and biophysics

authors： Pearson RB,Floyd DM,Hunt JT,Lee VG,Kemp BE

更新日期：1988-01-01 00:00:00

abstract：:The retinal pigment epithelium (RPE)-specific protein RPE65 is the major protein of the RPE microsomal membrane fraction. Though RPE65 lacks transmembrane domains or signal peptide, detergents are required for its maximally effective solubilization in isotonic buffers. However, in 0.75-1.0 M KCl, RPE65 is as soluble w...

journal_title：Archives of biochemistry and biophysics

authors： Tsilou E,Hamel CP,Yu S,Redmond TM

更新日期：1997-10-01 00:00:00

abstract：:Rat Leydig cells in primary culture were used as a model system to investigate the effects of human chorionic gonadotropin (hCG) and dibutyryl cyclic AMP (Bt2cAMP) on the synthesis of cholesterol side chain cleavage cytochrome P-450 (cytochrome P-450scc) and the iron-sulfur protein, adrenodoxin. Leydig cells isolated ...

journal_title：Archives of biochemistry and biophysics

authors： Anderson CM,Mendelson CR

更新日期：1985-05-01 00:00:00

abstract：:The ionization of 4-nitroimidazole to 4-nitroimidazolate was investigated as a function of ionic strength. The apparent pKa varies from 8.99 to 9.50 between 0.001 and 1.0 M ionic strength, respectively, at 25 degrees C. The ionic strength dependence of this ionization is anomalous. The binding of 4-nitroimidazole by h...

journal_title：Archives of biochemistry and biophysics

authors： Taylor KC,Vitello LB,Erman JE

更新日期：2000-10-15 00:00:00

abstract：:Gene expression in the obligate intracellular bacterium Chlamydia trachomatis ranges from nil in the infectious EB form to high in the dividing RB form. Little is known about the mechanisms of gene regulation in chlamydiae and only a few promoter sequences have been characterized. The purpose of our study was to exami...

journal_title：Archives of biochemistry and biophysics

authors： Shen L,Shi Y,Douglas AL,Hatch TP,O'Connell CM,Chen JM,Zhang YX

更新日期：2000-07-01 00:00:00

abstract：:Two new members of the polyketide synthase (PKS) gene family (RiPKS4 and RiPKS5) were cloned from raspberry fruits (Rubus idaeus L., cv Royalty) and expressed in Escherichia coli. Characterization of the recombinant enzyme products indicated that RiPKS4 is a bifunctional polyketide synthase producing both 4-hydroxyben...

journal_title：Archives of biochemistry and biophysics

authors： Zheng D,Hrazdina G

更新日期：2008-02-15 00:00:00

abstract：:Recently, we synthesized and characterized vanadyl saccharides to evaluate the effects of various vanadate and vanadyl complexes, which differ in their oxidation states on various biomacromolecules and cellular activities (1, 2). Here, we report that both vanadate (+V oxidation state) and different vanadyl species (+I...

journal_title：Archives of biochemistry and biophysics

authors： Krishnamoorthy T,Sreedhara A,Rao CP,Ramaiah KV

更新日期：1998-01-01 00:00:00

abstract：:Calorie restriction (CR) has been shown to improve peripheral insulin resistance and type 2 diabetes in animal models. However, the exact mechanism of CR on GLUT4 expression and translocation in insulin-sensitive tissues has not been well elucidated. In the present study, we examine the effect of CR on the expression ...

journal_title：Archives of biochemistry and biophysics

authors： Park SY,Choi GH,Choi HI,Ryu J,Jung CY,Lee W

更新日期：2005-04-15 00:00:00

abstract：:Providing clean and affordable drinking water without harmful disinfection byproducts generated by conventional chemical disinfectants gives rise to the need for technological innovation. Nanotechnology has great potential in purifying water and wastewater treatment. A graphene oxide-silver (GO-Ag) nanocomposite with ...

journal_title：Archives of biochemistry and biophysics

authors： Song B,Zhang C,Zeng G,Gong J,Chang Y,Jiang Y

更新日期：2016-08-15 00:00:00

abstract：:The hnRNP A/B family contains abundant nuclear proteins with major roles in alternative splicing and the ability for nucleo-cytoplasmic shuttling. Compared to the best known members of this family (hnRNP A1, A2/B1), hnRNP A3 is a relatively less known protein. We report herein immunochemical studies with the hnRNP A3 ...

journal_title：Archives of biochemistry and biophysics

authors： Papadopoulou C,Boukakis G,Ganou V,Patrinou-Georgoula M,Guialis A

更新日期：2012-07-15 00:00:00

abstract：:Prostaglandin E (PGE) stimulates resorption in bone. Since osteoblast-like osteosarcoma cells secrete PGE2, the possibility that osteoclasts were the major target for PGE was considered. To study this question, it was first established that in isolated bone cells enriched for either osteoclastic (OC) or osteoblastic (...

journal_title：Archives of biochemistry and biophysics

authors： Wong GL,Kocour BA

更新日期：1983-07-01 00:00:00

abstract：:The pH dependence of the activity of Cu,Zn superoxide dismutases from bovine erythrocytes and shark liver was studied by pulse radiolysis in both the native enzymes and those chemically modified at lysine side chains. The study was aimed at identifying the residues responsible for the activity decrease at pH > 9, obse...

journal_title：Archives of biochemistry and biophysics

authors： Polticelli F,O'Neill P,Costanzo S,Lania A,Rotilio G,Desideri A

更新日期：1995-08-01 00:00:00

abstract：:As a means of comparing the functional properties of an enzyme in dilute solution in vitro with those for the same enzyme acting in its normal cellular environment, a study was conducted with 4-substituted pyrazoles as inhibitors of rat liver alcohol dehydrogenase in vitro and ethanol oxidation in isolated rat hepatoc...

journal_title：Archives of biochemistry and biophysics

authors： Cornell NW,Hansch C,Kim KH,Henegar K

更新日期：1983-11-01 00:00:00

abstract：:Deoxycholic acid, a colon tumor promoter, was found to bind covalently to DNA and RNA in the presence of methyl linoleate hydroperoxide and ferrous ion. This binding was shown to occur specifically with guanine residues and its covalent nature was demonstrated by analysis of hydrolysates of nucleic acid adducts. These...

journal_title：Archives of biochemistry and biophysics

authors： Kodama M,Inoue F,Nakayama T,Nagata C

更新日期：1987-11-01 00:00:00

abstract：:Thromboxane A2 (TXA) is a potent vasoconstrictor and mediator of platelet aggregation. Thromboxane synthase (TXAS), which catalyzes the biosynthesis of TXA, is a member of the cytochrome P450 superfamily. We report here the complete genomic structure of the human TXAS gene. The gene contains 13 exons and is 193 kb lon...

journal_title：Archives of biochemistry and biophysics

authors： Tazawa R,Green ED,Ohashi K,Wu KK,Wang LH

更新日期：1996-10-15 00:00:00

abstract：:The relationships between conformational dynamics, stability and protein function are not obvious. Frataxin (FXN) is an essential protein that forms part of a supercomplex dedicated to the iron-sulfur (Fe-S) cluster assembly within the mitochondrial matrix. In humans, the loss of FXN expression or a decrease in its fu...

journal_title：Archives of biochemistry and biophysics

authors： Castro IH,Bringas M,Doni D,Noguera ME,Capece L,Aran M,Blaustein M,Costantini P,Santos J

更新日期：2020-09-30 00:00:00

abstract：:Forskolin, a diterpene that exerts several pharmacological effects, activates adenylate cyclase in brain and in some other mammalian tissues. Properties of forskolin activation of adenylate cyclase from central nervous system of the dipterous Ceratitis capitata are described. The interaction of forskolin with the inse...

journal_title：Archives of biochemistry and biophysics

authors： Guillen A,Haro A,Municio AM

更新日期：1987-04-01 00:00:00

abstract：:We isolated two new dihydrooxopyridine cross-links, oxodesmosine (OXD) and isooxodesmosine (IOXD) from the acid hydrolysates of the bovine aortic elastin. OXD and IOXD were identified to have N-substituted 1,2-dihydro-2-oxopyridine and N-substituted 1,4-dihydro-4-oxopyridine skeletons, respectively, with three alpha-a...

journal_title：Archives of biochemistry and biophysics

authors： Umeda H,Nakamura F,Suyama K

更新日期：2001-01-01 00:00:00

abstract：:The secretion of newly synthesized apolipoprotein E (apo E) by human monocyte-derived macrophages (HMD macrophages) was measured in the medium of cells which had been incubated for 24 h with or without either native or acetylated low-density lipoproteins (LDL or AcLDL, respectively), and subsequently with [35S]methion...

journal_title：Archives of biochemistry and biophysics

authors： Kayden HJ,Maschio F,Traber MG

更新日期：1985-06-01 00:00:00

abstract：:The neprilysin gene is composed of three distinct 5' noncoding exons which can be joined to the first coding exon to generate multiple mRNA species, all encoding the same protein. Genomic fragments containing upstream sequences of each of these three noncoding exons from the rat neprilysin gene were subcloned in the p...

journal_title：Archives of biochemistry and biophysics

authors： Li C,Hersh LB

更新日期：1998-10-01 00:00:00

abstract：:Cytochrome c has been purified to homogeneity from alligator liver (Alligator mississipiensis) using aluminum sulfate precipitation, CM-cellulose and gel-filtration chromatography, and reverse-phase HPLC. The protein exhibited a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an approxima...

journal_title：Archives of biochemistry and biophysics

authors： Barber MJ,Trimboli AJ,Clark M,Young C,Neame PJ

更新日期：1993-03-01 00:00:00

abstract：:Glucosylsphingosine is a potent inhibitor of lysosomal glucocerebrosidase and the broad-specificity, cytosolic beta-glucosidase of human liver. In the present study, it was demonstrated that the broad-specificity beta-glucosidase was also inhibited by galactosylsphingosine. The inhibition was observed when the enzyme ...

journal_title：Archives of biochemistry and biophysics

authors： LaMarco KL,Glew RH

更新日期：1985-02-01 00:00:00

abstract：:Recently, we characterized a surface antigen (Z-1) of guinea pig macrophages by monoclonal anti-Z-1 antibody. The Z-1 antigen consists of two different polypeptide chains; alpha (140 kDa) and beta (95 kDa). This antigen is closely correlated with the phagocytic activity of the cells for zymosan and presumably function...

journal_title：Archives of biochemistry and biophysics

authors： Hazeki K,Tamoto K,Tada M,Mori Y

更新日期：1989-05-01 00:00:00

abstract：:On Biorex 70 ion exchanger at neutral pH the histones H3 and H4 are usually eluted by 4 M guanidinium chloride (gdm Cl). In order to protect cysteines and methionines from oxidation we systematically added 2-mercaptoethanol to the elution buffer. This resulted in the two histones being unexpectedly eluted together at ...

journal_title：Archives of biochemistry and biophysics

authors： Couppez M,Belaïche D

更新日期：1996-01-01 00:00:00

abstract：:A series of mutant L12 ribosomal proteins was prepared by site-directed mutations in the L12 protein gene of the archaeon Sulfolobus acidocaldarius. The mutant protein genes were overexpressed in Escherichia coli, and the products purified and incorporated into ribosomal cores which had been ethanol extracted to remov...

journal_title：Archives of biochemistry and biophysics

authors： Kusser I,Lowing C,Rathlef C,Köpke AK,Matheson AT

更新日期：1999-05-15 00:00:00