Peroxynitrite signaling in human erythrocytes: synergistic role of hemoglobin oxidation and band 3 tyrosine phosphorylation.


:Peroxynitrite crosses the red blood cell (RBC) membrane and reacts with hemoglobin (Hb) producing mainly metHb, which is reduced back to ferrousHb by NADH- and NADPH-dependent reductases. Peroxynitrite also induces band 3 (B3) tyrosine phosphorylation, a signaling pathway believed to activate glucose metabolism. This study was aimed to decipher the relationship between these two peroxynitrite-dependent processes. Peroxynitrite induced a burst of the hexose monophosphate shunt (HMS), revealed by NMR studies, and a burst of the glycolytic pathway, measured by lactate production. The HMS plays a prominent role in membrane signaling, as demonstrated by B3 phosphotyrosine inhibition by the glycolytic pathway inhibitor 2-deoxy-glucose (2DG) and activation by dehydroepiandrosterone (DHEA), an inhibitor of HMS. Peroxynitrite-induced B3 tyrosine phosphorylation was paralleled by the inhibition of membrane-associated phosphotyrosine phosphatase (PTP) activity, which was protected by 2DG but not DHEA. Interestingly, heme poisoning with CO inhibited peroxynitrite-dependent Hb oxidation and lactate production but did not affect PTP down regulation. These results suggest two distinct and concurrent effects of peroxynitrite: one mediated by Hb which, likely in its oxidized state, binds more strongly to B3, and another mediated by PTP-dependent B3 phosphorylation. Both effects are directed towards a surge in glucose utilization.


Arch Biochem Biophys


Metere A,Iorio E,Pietraforte D,Podo F,Minetti M




Has Abstract


2009-04-15 00:00:00














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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Engberg P,Millqvist E,Pohl G,Lindskog S

    更新日期:1985-09-01 00:00:00

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Shen GQ,Kresbach G,Spiro MJ,Spiro RG

    更新日期:1995-08-01 00:00:00

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    pub_type: 杂志文章


    authors: Donnelly MI,Escalante-Semerena JC,Rinehart KL Jr,Wolfe RS

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    abstract::Nanoceria were synthesized by discharging plasma at 800 V with a frequency of 30 kHz for 0-25 min using a pulsed unipolar power supply into solutions containing 1 or 2 mM of Ce(NO3)2. UV-Vis spectroscopy showed a characteristic absorbance maxima at 304-320 nm for the nanoceria with increase in the intensity of the pea...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Davoodbasha M,Park BR,Rhee WJ,Lee SY,Kim JW

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lee HC,Lim ML,Lu CY,Liu VW,Fahn HJ,Zhang C,Nagley P,Wei YH

    更新日期:1999-02-15 00:00:00

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gundimeda U,Hara SK,Anderson WB,Gopalakrishna R

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Calabro A,Hascall VC,Caterson B

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    authors: Loureiro CA,Barros P,Matos P,Jordan P

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    journal_title:Archives of biochemistry and biophysics

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    authors: Carter RS,Avadhani NG

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  • Stereochemical studies of the C-methylation of deoxycytidine catalyzed by HhaI methylase and the N-methylation of deoxyadenosine catalyzed by EcoRI methylase.

    abstract::The steric course of methyl group transfer catalyzed by two DNA methylases, HhaI methylase, a DNA (cytosine-5)-methyltransferase, and EcoRI methylase, which methylates at N6 of adenosine, has been studied with (methyl-R)- and (methyl-S)-[methyl-2H1,3H]adenosylmethionine as the methyl donor, using as substrates poly-d(...

    journal_title:Archives of biochemistry and biophysics

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    authors: Ho DK,Wu JC,Santi DV,Floss HG

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  • Boswellic acids and their derivatives as potent regulators of glucocorticoid receptor actions.

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    authors: Karra AG,Tziortziou M,Kylindri P,Georgatza D,Gorgogietas VA,Makiou A,Krokida A,Tsialtas I,Kalousi FD,Papadopoulos GE,Papadopoulou KΚ,Psarra AG

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    journal_title:Archives of biochemistry and biophysics

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    authors: Mesbah NM

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Xu F,DeFilippi LJ,Ballou DP,Hultquist DE

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    pub_type: 杂志文章


    authors: Camadro JM,Abraham NG,Levere RD

    更新日期:1985-10-01 00:00:00

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    authors: Hernández F,López-Alarcón L,Puerta C,Palacián E

    更新日期:1998-10-01 00:00:00

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Szczesna-Skorupa E,Chen CD,Kemper B

    更新日期:2000-02-15 00:00:00

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    authors: Takikawa H,Arai S,Yamanaka M

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lee JC,Haley WT Jr

    更新日期:1983-07-01 00:00:00

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hu LY,Abeles RH

    更新日期:1990-09-01 00:00:00

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Yin JJ,Smith MJ,Eppley RM,Troy AL,Page SW,Sphon JA

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Borders CL Jr,Horton PJ,Beyer WF Jr

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    pub_type: 杂志文章


    authors: Giulivi C,Boveris A,Cadenas E

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Rana TM,Jeang KT

    更新日期:1999-05-15 00:00:00

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Salerno C,Capuozzo E

    更新日期:2013-09-01 00:00:00

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hicks M,Delbridge L,Yue DK,Reeve TS

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Xia T,Jensen RA

    更新日期:1992-04-01 00:00:00