Isolation and characterization of uteroglobin from the lung of the hare (Lepus capensis).

abstract：:The gene of the human fatty acid omega-hydroxylase, CYP4A11, has been isolated from a human BAC library, and its complete genomic sequence has been determined. The CYP4A11 gene spanned 12,568 bp and contained 12 exons. The known PPAR recognition elements (PPRE), which were reported to be involved in the induction of C...

journal_title：Archives of biochemistry and biophysics

authors： Kawashima H,Naganuma T,Kusunose E,Kono T,Yasumoto R,Sugimura K,Kishimoto T

更新日期：2000-06-15 00:00:00

abstract：:The pH gradient, delta pH, present across the inner mitochondrial membrane in isolated rat hepatocytes was continuously monitored with a novel spectroscopic technique that utilizes the weak acid fluorescein. Unlike most cytosolic pH indicators, such as 2',7'-bis(carboxyethyl)-5,(6)-carboxyfluorescein (BCECF), fluoresc...

journal_title：Archives of biochemistry and biophysics

authors： Thomas PJ,Gaspers LD,Pharr C,Thomas JA

更新日期：1991-07-01 00:00:00

abstract：:Hepatic cysteine sulfinic acid decarboxylase (EC 4.1.1.29) activity has been reported to decrease in response to both L-methionine (Met) feeding and adrenalectomy in rats. A series of experiments was conducted to (a) determine if CSAD depression was evident in female rats fed a methionine-supplemented diet; and (b) de...

journal_title：Archives of biochemistry and biophysics

authors： Jerkins AA,Steele RD

更新日期：1992-05-01 00:00:00

abstract：:It is established that high concentrations of nitric oxide(1) (NO), as released from activated macrophages, induce apoptosis in breast cancer cells. In this study, we assessed the potential of a light-activated NO donor [(Me2bpb)Ru(NO)(Resf)], a recently reported apoptototic agent, in suppressing the anchorage indepen...

journal_title：Archives of biochemistry and biophysics

authors： Sen S,Kawahara B,Fry NL,Farias-Eisner R,Zhang D,Mascharak PK,Chaudhuri G

更新日期：2013-12-01 00:00:00

abstract：:Immunological cross-reactivities between isolated subunits of the scorpion Androctonus australis (Aa) and of the horseshoe crab Limulus polyphemus (Lp) hemocyanins were studied using subunit-specific antibodies prepared through immunoadsorption to pure immobilized subunits. Rocket immunoelectrophoreses of the various ...

journal_title：Archives of biochemistry and biophysics

authors： Lamy J,Compin S,Lamy JN

更新日期：1983-06-01 00:00:00

abstract：:Maitotoxin (MTX) is a highly potent marine toxin that activates both voltage-sensitive and receptor-operated calcium channels in the plasma membrane. This results in calcium overload that rapidly leads to cell death. We now report that maitotoxin (0.1-1 nM) induces calpain activation in both SH-SY5Y neuroblastoma cell...

journal_title：Archives of biochemistry and biophysics

authors： Wang KK,Nath R,Raser KJ,Hajimohammadreza I

更新日期：1996-07-15 00:00:00

abstract：:The tumor-promoting phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA) inhibited thrombin-stimulated arachidonic acid (AA) release in rabbit and human platelets. PMA was effective over the same concentration range that activates protein kinase C in intact rabbit platelets: IC50 vs thrombin = 0.5 nM, greater th...

journal_title：Archives of biochemistry and biophysics

authors： Murayama T,Kajiyama Y,Takahashi A,Nomura Y

更新日期：1990-01-01 00:00:00

abstract：:The mechanism by which dietary cholesterol regulates expression of the hepatic low-density lipoprotein (LDL) receptor was investigated. In a previous study (Arch. Biochem. Biophys. 325, 242-248, 1996), we demonstrated that dietary cholesterol reduces the rate of LDL receptor protein degradation without affecting stead...

journal_title：Archives of biochemistry and biophysics

authors： Lopez D,Ness GC

更新日期：1997-08-01 00:00:00

abstract：:The three-dimensional conformation of a protein provides a wealth of biochemical information and with the advent of cloning techniques that allow the preparation of proteins almost at will, a renewed interest has arisen in the crystallographic determination of protein structures. As in any research technique, however,...

journal_title：Archives of biochemistry and biophysics

authors： Holden HM,Rayment I

更新日期：1991-11-15 00:00:00

abstract：:This report describes the post-translational modifications of recombinant human differentiation-stimulating factor, a 180-residue glycoprotein that is secreted from transfected Chinese hamster ovary cells. Peptide peptides containing six potential N-glycosylation sites were analyzed to determine that Asn residues 9, 3...

journal_title：Archives of biochemistry and biophysics

authors： Schmelzer CH,Harris RJ,Butler D,Yedinak CM,Wagner KL,Burton LE

更新日期：1993-05-01 00:00:00

abstract：:A chromatographic adsorbent (TED-Sepharose) capable of chelating various di- and trivalent metal ions was prepared by introducing tris(carboxymethyl)ethylenediamine into agarose. This gel was charged with Al3+, Ga3+, In3+, and Tl3+ to form immobilized-metal affinity adsorbents, and their adsorption behavior toward ser...

journal_title：Archives of biochemistry and biophysics

authors： Porath J,Olin B,Granstrand B

更新日期：1983-09-01 00:00:00

abstract：:We previously reported that administration of dexamethasone (DEX) and other selected pharmacological agents to rats resulted in a profound increase in hepatic cytochrome P450 3A1 in both sexes, but male constitutive P450 3A2 was modestly increased (4-fold) in adult males and not detected in either treated or untreated...

journal_title：Archives of biochemistry and biophysics

authors： Ghosal A,Sadrieh N,Reik L,Levin W,Thomas PE

更新日期：1996-08-01 00:00:00

abstract：:The phosphorylation state of several cardiac myofilament proteins changes with the level of stretch in intact, twitch-contracting cardiac muscles. It remains unclear which kinases are involved in the length-dependent phosphorylation of these proteins. We set out to investigate which kinases are involved after a step-w...

journal_title：Archives of biochemistry and biophysics

authors： Monasky MM,Taglieri DM,Jacobson AK,Haizlip KM,Solaro RJ,Janssen PM

更新日期：2013-07-01 00:00:00

abstract：:From our topological arrangement model of prostaglandin I(2) synthase (PGIS) created by homology modeling and topology studies, we hypothesized that the helix F/G loop of PGIS contains a membrane contact region distinct from the N-terminal membrane anchor domain. To provide direct experimental data we have explored th...

journal_title：Archives of biochemistry and biophysics

authors： Wu J,So SP,Ruan KH

更新日期：2003-03-01 00:00:00

abstract：:Xenopus laevis nucleoplasmin is a pentameric nuclear chaperone. The relation between the structure and the multifunctional aspects of the molecule has not yet been clearly established. In the course of analysing a C-terminally His-tagged recombinant version of the region equivalent to the trypsin resistant core (r-NP1...

journal_title：Archives of biochemistry and biophysics

authors： Arnan C,Prieto C,Chiva M,Salvany L,Ausió J,Subirana JA,Saperas N

更新日期：2005-05-15 00:00:00

abstract：:The ability for various ligands to modulate the binding of fructose 1,6-bisphosphate (Fru-1,6-P2) with purified rat liver pyruvate kinase was examined. Binding of Fru-1,6-P2 with pyruvate kinase exhibits positive cooperativity, with maximum binding of 4 mol Fru-1,6-P2 per enzyme tetramer. The Hill coefficient (nH), an...

journal_title：Archives of biochemistry and biophysics

authors： Blair JB,Walker RG

更新日期：1984-07-01 00:00:00

abstract：:Hyaluronidase from fresh human serum was purified to apparent homogeneity in a two-step procedure. Potent serum inhibitors of hyaluronidase activity were removed during the course of the purification. Isolation of the enzyme was expedited by the use of a newly devised ELISA-like assay. Enzyme activity was measured by ...

journal_title：Archives of biochemistry and biophysics

authors： Afify AM,Stern M,Guntenhöner M,Stern R

更新日期：1993-09-01 00:00:00

abstract：:Peroxisomes contain oxidases that produce H(2)O(2), which can result in protein oxidation. To test the vulnerability of peroxisomal proteins to oxidation in vivo the organelles were isolated from castor bean endosperm incubated with H(2)O(2). When peroxisomes were exposed to H(2)O(2)in vivo, the peroxisomal proteins e...

journal_title：Archives of biochemistry and biophysics

authors： Anand P,Kwak Y,Simha R,Donaldson RP

更新日期：2009-11-01 00:00:00

abstract：:Two histone acetyltransferases, A and B, have been extracted and partially purified from yeast cells. The purification scheme included ammonium sulfate precipitation, and chromatography on DEAE-Sepharose and Sephadex G-200. The basic properties of both enzymes closely correspond to those of acetyltransferase A and B f...

journal_title：Archives of biochemistry and biophysics

authors： Lopez-Rodas G,Perez-Ortin JE,Tordera V,Salvador ML,Franco L

更新日期：1985-05-15 00:00:00

abstract：:Recombinant human lymphotoxin (rhLT) produced by CHO cells transfected with human LT genomic DNA was purified to homogeneity, but approximately 5% of the molecules were devoid of the last two amino terminal residues. A peptide N-glycosylated at Asn62 (Tr-45) and one partially O-glycosylated at Thr7 (Tr-14) on cleavage...

journal_title：Archives of biochemistry and biophysics

authors： Fukushima K,Watanabe H,Takeo K,Nomura M,Asahi T,Yamashita K

更新日期：1993-07-01 00:00:00

abstract：:I had the fortune to be introduced to Helmut Sies during the mid 1980s, while working as a post-doctoral scientist at the University of California, Berkeley. At that time, Helmut was a frequent visitor of the Bruce Ames' laboratory and a leading authority in antioxidants and oxidative stress. His concepts, ideas and w...

journal_title：Archives of biochemistry and biophysics

authors： Stocker R

更新日期：2016-04-01 00:00:00

abstract：:While the general catalytic mechanism of the widespread serine hydrolase superfamily has been documented extensively, much less is known about its varied modes of functional modulation within biological systems. Under oxidizing conditions, inhibition of Saccharomyces cerevisiae S-formylglutathione hydrolase (SFGH, hom...

journal_title：Archives of biochemistry and biophysics

authors： Legler PM,Leary DH,Hervey WJ 4th,Millard CB

更新日期：2012-12-01 00:00:00

abstract：:Physicochemical properties and catalytic activity of cytochrome P4501A2 in the reconstituted system with Emulgen 913 were studied. The formation of cytochrome P4501A2 monomers was shown by gel filtration at an Emulgen concentration of 8 g/liter. The catalytic activity of the monomeric monooxygenase system was low. The...

journal_title：Archives of biochemistry and biophysics

authors： Sevrukova IF,Kanaeva IP,Koen YM,Samenkova NF,Bachmanova GI,Archakov AI

更新日期：1994-05-15 00:00:00

abstract：:Glaucoma is the leading cause of irreversible blindness in the world and trabeculectomy remains still the most commonly performed filtration surgery. Failure of trabeculectomy is due to the formation of scarring, which is associated with the increased fibroblast proliferation, activation, and collagen deposition at th...

journal_title：Archives of biochemistry and biophysics

authors： Wang L,Guo D,Wang Z,Yin X,Wei H,Hu W,Chen R,Chen C

更新日期：2020-04-15 00:00:00

abstract：:Sequence analyses of the C-terminal membrane intercalative region of the rat cytochrome b(5) indicated that this domain has, in addition to a signal sequence, a combined element of the classic stop-transfer sequence typically found in a variety of transmembrane proteins. Such bitopic protein arrangements arise by tand...

journal_title：Archives of biochemistry and biophysics

authors： Kaderbhai MA,Morgan R,Kaderbhai NN

更新日期：2003-04-15 00:00:00

abstract：:Human tumor necrosis factor (TNF), first isolated by our group as an anticancer agent, has been now shown to be a primary mediator of inflammation. Till today 19 different members of the TNF superfamily which interact with 29 different receptors, have been identified. Most members of this family exhibit pro-inflammato...

journal_title：Archives of biochemistry and biophysics

authors： Gupta SC,Tyagi AK,Deshmukh-Taskar P,Hinojosa M,Prasad S,Aggarwal BB

更新日期：2014-10-01 00:00:00

abstract：:Kirsten murine sarcoma virus-transformed Balb/c 3T3 cells (KiMSV) are highly tumorigenic and metastatic in the appropriate murine host, are loosely adherent to the tissue culture substratum, and can be readily detached from the substratum by ethylene glycol bis(beta-aminoethyl ether) N,N'-tetraacetic acid treatment le...

journal_title：Archives of biochemistry and biophysics

authors： Miketo LJ,Culp LA

更新日期：1983-03-01 00:00:00

abstract：:Nickel (Ni) is widely present in the occupational environment and causes various adverse effects on the human body. Apoptosis induced by Ni2+ may be a key mechanism underlying its toxic effect. In the present study, we investigated the effect of Ni-smelting fumes on cell viability, mitochondrial damage, and apoptosis-...

journal_title：Archives of biochemistry and biophysics

authors： Pan YL,Xin R,Wang SY,Wang Y,Zhang L,Yu CP,Wu YH

更新日期：2018-12-15 00:00:00

abstract：:The actions of polycyclic aromatic hydrocarbons and glucocorticoids to regulate the expression of cytochrome P450c were investigated using cultured fetal rat hepatocytes. Cytochrome P450c mRNA content, determined by Northern blot analysis, was induced in cells treated with 1,2-benzanthracene from levels undetectable i...

journal_title：Archives of biochemistry and biophysics

authors： Mathis JM,Houser WH,Bresnick E,Cidlowski JA,Hines RN,Prough RA,Simpson ER

更新日期：1989-02-15 00:00:00

abstract：:Nitration of protein tyrosine residues to 3-nitrotyrosine (NO2Tyr) serves as both a marker and mediator of pathogenic reactions of nitric oxide (*NO), with peroxynitrite (ONOO-) and leukocyte peroxidase-derived nitrogen dioxide (*NO2) being proximal mediators of nitration reactions in vivo. Cytochrome c is a respirato...

journal_title：Archives of biochemistry and biophysics

authors： Castro L,Eiserich JP,Sweeney S,Radi R,Freeman BA

更新日期：2004-01-01 00:00:00