Maitotoxin induces calpain activation in SH-SY5Y neuroblastoma cells and cerebrocortical cultures.

abstract：:The crystalline beef liver protein of Sumner and Dounce (A. L. Dounce, P. Z. Allen, and G. A. Mourtzikos (1978) Arch. Biochem. Biophys. 188, 251-265) termed FTBL (football) protein because of the shape of its crystals, has been identified as a crystalline leucine aminopeptidase (LAP), on the basis of its high specific...

journal_title：Archives of biochemistry and biophysics

authors： Dounce AL,Allen PZ

更新日期：1987-08-15 00:00:00

abstract：:By anion-exchange chromatography, the nitrogen fixing photosynthetic stem nodules and nonphotosynthetic root nodules of Sesbania rostrata are shown to contain the same seven major components of leghemoglobin (Lb), numbered LbI-LbVII in order of elution, although in different proportions. No novel component was found i...

journal_title：Archives of biochemistry and biophysics

authors： Bogusz D,Kortt AA,Appleby CA

更新日期：1987-04-01 00:00:00

abstract：:The aim of the present study was to further understand how changes in membrane organization can lead to higher rates of lipid oxidation. We previously demonstrated that Al(3+), Sc(3+), Ga(3+), Be(2+), Y(3+), and La(3+) promote lipid packing and lateral phase separation. Using the probe Laurdan, we evaluated in liposom...

journal_title：Archives of biochemistry and biophysics

authors： Verstraeten SV,Oteiza PI

更新日期：2000-03-15 00:00:00

abstract：:A set of membrane proteins from murine myelomonocytic leukemia cell line WEHI-3BD+ was found to be ADP-ribosylated. Both this ADP-ribosylation reaction and granulocytic differentiation in response to recombinant G-CSF were inhibited approximately 50% by 2 mM benzamide, suggesting a correlation between these two proces...

journal_title：Archives of biochemistry and biophysics

authors： Hanson K,Aboul-Ela N,Jacobson MK,Wu MC

更新日期：1993-04-01 00:00:00

abstract：:The structure of rabbit muscle pyruvate kinase crystallized as a complex with Mg2+, K+, and L-phospholactate (L-P-lactate) has been solved and refined to 2.7 A resolution. The crystals, grown from solutions of polyethylene glycol 8000 at pH 7.5, belong to the space group P2(1) and have unit cell parameters a = 144.4 A...

journal_title：Archives of biochemistry and biophysics

authors： Larsen TM,Benning MM,Wesenberg GE,Rayment I,Reed GH

更新日期：1997-09-15 00:00:00

abstract：:Nickel (Ni) is widely present in the occupational environment and causes various adverse effects on the human body. Apoptosis induced by Ni2+ may be a key mechanism underlying its toxic effect. In the present study, we investigated the effect of Ni-smelting fumes on cell viability, mitochondrial damage, and apoptosis-...

journal_title：Archives of biochemistry and biophysics

authors： Pan YL,Xin R,Wang SY,Wang Y,Zhang L,Yu CP,Wu YH

更新日期：2018-12-15 00:00:00

abstract：:Hyaluronidase from fresh human serum was purified to apparent homogeneity in a two-step procedure. Potent serum inhibitors of hyaluronidase activity were removed during the course of the purification. Isolation of the enzyme was expedited by the use of a newly devised ELISA-like assay. Enzyme activity was measured by ...

journal_title：Archives of biochemistry and biophysics

authors： Afify AM,Stern M,Guntenhöner M,Stern R

更新日期：1993-09-01 00:00:00

abstract：:Intracellular glutathione (GSH) depletion induced by buthionine sulfoximine (BSO) caused cell death that seemed to be apoptosis in C6 rat glioma cells. Arachidonic acid (AA) promoted BSO-induced cell death by accumulating reactive oxygen species (ROS) or hydroperoxides. AA inhibited caspase-3 activation and internucle...

journal_title：Archives of biochemistry and biophysics

authors： Higuchi Y,Yoshimoto T

更新日期：2002-04-01 00:00:00

abstract：:There is a current interest in dietary compounds (such as trans-resveratrol) that can inhibit or reverse oxidative stress, the common pathway for a variety of brain disorders, including Alzheimer's disease and stroke. The objective of the present study was to investigate the effects of resveratrol, under conditions of...

journal_title：Archives of biochemistry and biophysics

authors： de Almeida LM,Leite MC,Thomazi AP,Battu C,Nardin P,Tortorelli LS,Zanotto C,Posser T,Wofchuk ST,Leal RB,Gonçalves CA,Gottfried C

更新日期：2008-12-01 00:00:00

abstract：:Chloride ion is found to be an essential factor in photoreactivation of the oxygen-evolving center. Tris-washed, 2,6-dichlorophenol indophenol-treated grana are low in both Mn content and oxygen-evolving activity. These grana can restore high oxygen-evolving activity, however, by incorporating Mn2+ ion under weak ligh...

journal_title：Archives of biochemistry and biophysics

authors： Yamashita T,Ashizawa A

更新日期：1985-05-01 00:00:00

abstract：:Chronic kidney disease (CKD) is an increasingly recognized burden for patients and health care systems with high (and growing) global incidence and prevalence, significant mortality, and disproportionately high treatment costs. Yet, the available diagnostic tools are either impractical in clinical routine or have seri...

journal_title：Archives of biochemistry and biophysics

authors： Breit M,Weinberger KM

更新日期：2016-01-01 00:00:00

abstract：:Substrate specificity of human mitochondrial low Km aldehyde dehydrogenase (EC 1.2.1.3) E2 isozyme has been investigated employing p-nitrophenyl esters of acyl groups of two to six carbon atoms and comparing with that of aldehydes of one to eight carbon atoms. The esterase reaction was studied under three conditions: ...

journal_title：Archives of biochemistry and biophysics

authors： Mukerjee N,Pietruszko R

更新日期：1992-11-15 00:00:00

abstract：:An anionic peroxidase (EC 1.11.1.7), thought to be involved in suberization, was purified 110-fold from wound-healing slices of Solanum tuberosum by a combination of ammonium sulfate fractionation, Sephadex G-100 gel filtration, isoelectric focusing, and phenyl-Sepharose CL-4B chromatography in 24% yield. The purified...

journal_title：Archives of biochemistry and biophysics

authors： Espelie KE,Kolattukudy PE

更新日期：1985-08-01 00:00:00

abstract：:The major phenobarbital-inducible form of cytochrome P-450 (cytochrome P-450 PB) was purified to homogeneity from rat liver microsomes and rabbit antibodies prepared against the purified enzyme. Using these antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed for the detection of cytochrome P-450 PB ...

journal_title：Archives of biochemistry and biophysics

authors： Seidel SL,Shawver LK,Shires TK

更新日期：1984-03-01 00:00:00

abstract：:The treatment of male rats with Hg2+ resulted in significant alterations in heme and hemoprotein metabolism in the adrenal gland which, in turn, were reflected in abnormal steroidogenic activities and steroid output. Twenty-four hours after the administration of 30 mumol of HgCl2/kg (sc) the mitochondrial heme and cyt...

journal_title：Archives of biochemistry and biophysics

authors： Veltman JC,Maines MD

更新日期：1986-08-01 00:00:00

abstract：:The fugu (pufferfish) genome has been sequenced, and a second genome assembly was released 17 May 2002. Exhaustive searches were made to identify all P450 genes and pseudogenes from the earlier release of 26 October 2001. P450 genes assembled as completely as possible from these data were used to do additional searche...

journal_title：Archives of biochemistry and biophysics

authors： Nelson DR

更新日期：2003-01-01 00:00:00

abstract：:The effects of polyunsaturated fatty acids and lipid peroxidation on LM fibroblast plasma membrane individual leaflet sterol distribution and structural order were examined. The cytofacial (inner) leaflet was more rigid and contained more sterol than the exofacial (outer) leaflet. The static (limiting anisotropy) and ...

journal_title：Archives of biochemistry and biophysics

authors： Schroeder F,Kier AB,Sweet WD

更新日期：1990-01-01 00:00:00

abstract：:Catalases are biotechnologically relevant enzymes because of their applications in food technology, bioremediation, and biomedicine. The dismutation of hydrogen peroxide occurs in two steps; in the first one, the enzyme forms an oxidized compound I (Cpd I) and in the second one, the enzyme is reduced to the ferric sta...

journal_title：Archives of biochemistry and biophysics

authors： Zárate-Romero A,Stojanoff V,Cohen AE,Hansberg W,Rudiño-Piñera E

更新日期：2019-05-15 00:00:00

abstract：:Phosphatidylserine (PS) is predominantly confined to the inner leaflet of plasma membrane in cells, but it is externalized on the cell surface during apoptosis. This externalized PS is required for effective phagocytosis of apoptotic cells by macrophages. Because PS trans-bilayer asymmetry is not absolute in different...

journal_title：Archives of biochemistry and biophysics

authors： Borisenko GG,Matsura T,Liu SX,Tyurin VA,Jianfei J,Serinkan FB,Kagan VE

更新日期：2003-05-01 00:00:00

abstract：:The so-called 90,000-Da heat shock protein (hsp90) from chicken liver has been purified and physically characterized in the presence of high levels of the serine phosphatase inhibitor fluoride. The protein is an elongated dimer with a molecular weight of 160,000 and a frictional ratio of 1.6. On two-dimensional electr...

journal_title：Archives of biochemistry and biophysics

authors： Iannotti AM,Rabideau DA,Dougherty JJ

更新日期：1988-07-01 00:00:00

abstract：:It was found that the rate of polymerization of G-actin increased with the decrease of ATP concentration. When excess ATP was replaced by chloride through anion-exchange treatment, the extent of actin polymerization did not change provided that the ionic strength was raised immediately after the treatment. In the mean...

journal_title：Archives of biochemistry and biophysics

authors： Fung BM,Eyob E

更新日期：1983-02-01 00:00:00

abstract：:The effect of copper sulfate (CuSO4) on both hepatic oxidative stress and heme oxygenase induction was studied. A strong increase in in vivo rat liver chemiluminescence was observed 1 h after Cu(II) administration. To evaluate liver antioxidant enzymatic defenses, superoxide dismutase, catalase, and glutathione peroxi...

journal_title：Archives of biochemistry and biophysics

authors： Ossola JO,Groppa MD,Tomaro ML

更新日期：1997-01-15 00:00:00

abstract：:When applied in liquid paraffin to the upper surface of expanding spinach leaves, [1-14C]palmitic acid was efficiently and exclusively incorporated into the sn-1 position of cellular glycerolipids, principally phosphatidylcholine and triacylglycerol. A slow transfer of fatty acids from phosphatidylcholine to chloropla...

journal_title：Archives of biochemistry and biophysics

authors： Roughan PG,Thompson GA Jr,Cho SH

更新日期：1987-12-01 00:00:00

abstract：:Activation of the NLRP3 inflammasome plays an important role in high glucose- induced endothelial dysfunction in patients with type 2 diabetes mellitus (T2DM). Dulaglutide, a newly developed glucagon-like peptide-1 receptor (GLP-1R) agonist, has been approved for the management of T2DM. In the current study, we aimed ...

journal_title：Archives of biochemistry and biophysics

authors： Luo X,Hu Y,He S,Ye Q,Lv Z,Liu J,Chen X

更新日期：2019-08-15 00:00:00

abstract：:This work studied the effect of cholesteryl hemisuccinate incorporation on membrane fluidity and on the kinetics of oxygen diffusion at different depths in the erythrocyte membrane. Cholesterol concentration in the membrane was expressed as the cholesterol-protein ratio (C/Pt). The membrane fluidity, as assessed by a ...

journal_title：Archives of biochemistry and biophysics

authors： Dumas D,Muller S,Gouin F,Baros F,Viriot ML,Stoltz JF

更新日期：1997-05-01 00:00:00

abstract：:The tandem repeat 23-residue sequence [TRS23 (145-167): T-T-A-A-P-P-T-P-S-A-T-T-P-A-P-P-S-S-S-A-P-P-E] of human salivary mucin glycoprotein MG2 was examined for its in vitro bactericidal activity against four oral microorganisms, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus gordonii, a...

journal_title：Archives of biochemistry and biophysics

authors： Antonyraj KJ,Karunakaran T,Raj PA

更新日期：1998-08-15 00:00:00

abstract：:Triosephosphate isomerase from rabbit has 5 Cys and 2 Met, while triosephosphate isomerase from yeast has 2 Cys (present in the rabbit enzyme in equivalent positions) and no Met. Since chloramine-T oxidizes Cys and Met, we determined the effect it has on the activity and structure of both enzymes. The activity of trio...

journal_title：Archives of biochemistry and biophysics

authors： Zubillaga RA,Pérez-Montfort R,Gómez-Puyou A

更新日期：1994-09-01 00:00:00

abstract：:Cofactor and tryptophan accessibility of the 65-kDa form of rat brain glutamate decarboxylase (GAD) was investigated by fluorescence quenching measurements using acrylamide, I-, and Cs+ as the quenchers. Trp residues were partially exposed to solvent. I- was less able and Cs+ was more able to quench the fluorescence o...

journal_title：Archives of biochemistry and biophysics

authors： Rust E,Martin DL,Chen CH

更新日期：2001-08-15 00:00:00

abstract：:Much of what is known about the antioxidant mechanism of vitamin E has been learned from studies of lipid dispersions, solutions, or subcellular organelles. We have investigated the effect of vitamin E supplementation on intact live eucaryotic cells. L1210 murine leukemia cells were exposed to an oxidative stress indu...

journal_title：Archives of biochemistry and biophysics

authors： Wagner BA,Buettner GR,Burns CP

更新日期：1996-10-15 00:00:00

abstract：:The NADH-dependent microsomal electron transfer system consists of NADH-cytochrome b5 reductase and cytochrome b5, which donates reducing equivalents to fatty acyl desaturase, cytochrome P450, and other reactions. A study was carried out to investigate the interaction of NADH with several ferric complexes and to evalu...

journal_title：Archives of biochemistry and biophysics

authors： Yang MX,Cederbaum AI

更新日期：1995-12-20 00:00:00