Human mitochondrial aldehyde dehydrogenase substrate specificity: comparison of esterase with dehydrogenase reaction.


:Substrate specificity of human mitochondrial low Km aldehyde dehydrogenase (EC E2 isozyme has been investigated employing p-nitrophenyl esters of acyl groups of two to six carbon atoms and comparing with that of aldehydes of one to eight carbon atoms. The esterase reaction was studied under three conditions: in the absence of coenzyme, in the presence of NAD (1 mM), and in the presence of NADH (160 microM). The maximal velocity of the esterase reaction with p-nitrophenyl acetate and propionate as substrates in the presence of NAD was 3.9-4.7 times faster than that of the dehydrogenase reaction. Under all other conditions the velocities of dehydrogenase and esterase reactions were similar; the lowest kcat was for p-nitrophenyl butyrate in the presence of NAD. Stimulation of esterase activity by coenzymes was confined to esters of short acyl chain length; with longer acyl chain lengths or increased bulkiness (p-nitrophenyl guanidinobenzoate) no effect or even inhibition was observed. Comparison of kinetic constants for esters demonstrates that p-nitrophenyl butyrate is the worst substrate of all esters tested, suggesting that the active site topography is uniquely unfavorable for p-nitrophenyl butyrate. This fact is, however, not reflected in kinetic constants for butyraldehyde, which is a good substrate. The substrate specificity profile as determined by comparison of kcat/Km ratios was found to be quite different for aldehydes and esters. For aldehydes kcat/Km ratios increased with the increase of chain length; with esters under all three conditions, a V-shaped curve was produced with a minimum at p-nitrophenyl butyrate.


Arch Biochem Biophys


Mukerjee N,Pietruszko R




Has Abstract


1992-11-15 00:00:00














  • Transient adaptation to oxidative stress in yeast.

    abstract::Adaptive responses to the oxidative stress of hydrogen peroxide (H2O2) were studied in the yeast Saccharomyces cerevisciae strain RZ53. Our results show that the growth of naive cells is readily arrested by H2O2 challenge. In contrast, cells that have been preexposed to relatively low H2O2 priming treatments (i.e., ce...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Davies JM,Lowry CV,Davies KJ

    更新日期:1995-02-20 00:00:00

  • Structural details of the human cytochrome P-450c gene.

    abstract::Aryl hydrocarbon hydroxylase activity is most closely associated with cytochrome P-450c in the rat and cytochrome P1-450 in the mouse. The sequence for the orthologous human gene coding for this enzymatic activity has been determined from several sources: cytochrome P-450c isolated from human embryonic DNA [K. Kawajir...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Iversen PL,Heiger WJ,Bresnick E,Hines RN

    更新日期:1987-07-01 00:00:00

  • The use of 6-(difluoromethyl)indole to study the activation of indole by tryptophan synthase.

    abstract::6-(Difluoromethyl)indole has been characterized and developed as a probe for the turnover of indole by the bifunctional enzyme, tryptophan synthase (alpha 2 beta 2). The neutral form of the indolyl species undergoes a slow and spontaneous hydrolysis to produce 6-formylindole with a rate constant (k1) of 0.0089 +/- 0.0...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Woolridge EM,Rokita SE

    更新日期:1991-05-01 00:00:00

  • Molecular evolution and structure--function relationships of the superoxide dismutase gene families in angiosperms and their relationship to other eukaryotic and prokaryotic superoxide dismutases.

    abstract::This study assesses whether the phylogenetic relationships between SODs from different organisms could assist in elucidating the functional relationships among these enzymes from evolutionarily distinct species. Phylogenetic trees and intron positions were compared to determine the relationships among these enzymes. A...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Fink RC,Scandalios JG

    更新日期:2002-03-01 00:00:00

  • Cyclosporin A-sensitive permeability transition pore is involved in Cd(2+)-induced dysfunction of isolated rat liver mitochondria: doubts no more.

    abstract::There is dose-dependent Cd(2+)-evoked swelling of isolated rat liver mitochondria energized by complex I, II, or IV respiratory substrates in sucrose medium in the absence of added Ca(2+) and P(i), which is prevented by Sr(2+). Permeability transition effectors (ADP, CsA, EGTA, RR, DTT, ATR, P(i), and Ca(2+)) affect i...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Belyaeva EA,Glazunov VV,Korotkov SM

    更新日期:2002-09-15 00:00:00

  • Novel antiangiogenic agents in dermatology.

    abstract::Because angiogenesis underlies the pathogenesis of numerous conditions (cancer, psoriasis, macular degeneration), there is a pressing need for continued investigations into angiogenic signaling and potential drug targets. Antiangiogenic agents can be classified as either direct or indirect. Direct antiangiogenics act ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Berrios RL,Arbiser JL

    更新日期:2011-04-15 00:00:00

  • Biochemical and immunological identity of the hepatic peroxisomal and microsomal trans-2-enoyl CoA hydratase bifunctional protein.

    abstract::In the present study, the hepatic microsomal and peroxisomal bifunctional trans-2-enoyl CoA hydratases were isolated and purified from rats treated with 2% di-(2-ethylhexyl)phthalate for 8 days. These two enzymes (microsomal and peroxisomal) were purified with the identical purification procedures and had identical mo...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Cook L,Ghesquier D,Nagi MN,Favreau LV,Cinti DL

    更新日期:1987-02-01 00:00:00

  • Characterization of the branching patterns of glycogen branching enzyme truncated on the N-terminus.

    abstract::Truncation of 112 amino acids at the N-terminus (Nd(1-112)) changes the chain transfer pattern of the Escherichia coli glycogen branching enzyme (GBE) [Arch. Biochem. Biophys. 397 (2002) 279]. We investigated further the role of the N-terminus by engineering other truncated GBEs and analyzing the branching pattern by ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Devillers CH,Piper ME,Ballicora MA,Preiss J

    更新日期:2003-10-01 00:00:00

  • Isolation of iron-containing superoxide dismutase from Bacteroides fragilis: reconstitution as a Mn-containing enzyme.

    abstract::Superoxide dismutase from the anaerobe Bacteroides fragilis has been purified to apparent homogeneity. The protein, Mr 42,000, is a dimer of equally sized subunits joined by noncovalent interactions. Metal analysis of the native enzyme revealed 1.8-1.9 g-atoms Fe, 0.2 g-atoms Zn, and less than 0.05 g-atoms Mn per mole...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gregory EM,Dapper CH

    更新日期:1983-01-01 00:00:00

  • Activation of deoxycholic acid by the lipid peroxy radical and its covalent binding to nucleic acids.

    abstract::Deoxycholic acid, a colon tumor promoter, was found to bind covalently to DNA and RNA in the presence of methyl linoleate hydroperoxide and ferrous ion. This binding was shown to occur specifically with guanine residues and its covalent nature was demonstrated by analysis of hydrolysates of nucleic acid adducts. These...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kodama M,Inoue F,Nakayama T,Nagata C

    更新日期:1987-11-01 00:00:00

  • Upregulation of α2-adrenoceptor synthesis in SHR cardiomyocytes: Recompense without sense - Increased amounts, impaired commands.

    abstract:AIMS:to investigate α2-AR subtype distribution and the relationship between receptor amounts and their functionality in normotensive and spontaneously hypertensive rats. METHODS:experiments were performed on left ventricular cardiomyocytes isolated from Wistar rats and SHR (2-2.5 months). Molecular routine tools (RT-P...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kokoz YM,Evdokimovskii EV,Maltsev AV

    更新日期:2019-10-15 00:00:00

  • Group IB phospholipase A2 from Pseudonaja textilis.

    abstract::Pseudonaja textilis, an Australian Elapid, is known to produce a highly toxic venom. Both protein profiling and N-terminal sequence analysis showed the presence of four new phospholipases A(2) in this venom. Besides being non-lethal, the phospholipase A(2) proteins were found to be moderately active enzymes and they s...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Armugam A,Gong N,Li X,Siew PY,Chai SC,Nair R,Jeyaseelan K

    更新日期:2004-01-01 00:00:00

  • Glutamine transport in normal and acidotic rat kidney mitochondria.

    abstract::Glutamine transport in both normal and acidotic rat kidney mitochondria was investigated using both isotopic techniques and by spectroscopic measurements in which glutamine metabolism was allowed to occur. Widely used criteria for demonstrating the occurrence of carrier-mediated transport were successfully applied in ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Atlante A,Passarella S,Minervini GM,Quagliariello E

    更新日期:1994-12-01 00:00:00

  • Genomic organization and structure of alpha-hydroxynitrile lyase in cassava (Manihot esculenta Crantz).

    abstract::Two clones with homology to the alpha-hydroxynitrile lyase (HNL) cDNA clone, MeHNL10, were isolatedfrom a lambdaEMBL3 cassava (Manihot esculenta Crantz) genomic library. Analysis of the sequences showed that both genomic clones contain HNL genes (MeHNL4, MeHNL24) which are interrupted by two introns. RT-PCR analysis o...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hughes J,Keresztessy Z,Brown K,Suhandono S,Hughes MA

    更新日期:1998-08-15 00:00:00

  • The antioxidant and pro-oxidant activities of green tea polyphenols: a role in cancer prevention.

    abstract::Green tea (Camellia sinensis) is rich in catechins, of which (-)-epigallocatechin-3-gallate (EGCG) is the most abundant. Studies in animal models of carcinogenesis have shown that green tea and EGCG can inhibit tumorigenesis during the initiation, promotion and progression stages. Many potential mechanisms have been p...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Lambert JD,Elias RJ

    更新日期:2010-09-01 00:00:00

  • Cationomycin and monensin partition between serum proteins and erythrocyte membrane: consequences for Na+ and K+ transport and antimalarial activities.

    abstract::The ionophore properties of cationomycin and monensin were studied on human erythrocytes by measuring Na+ influx by 23Na NMR and concomitant K+ efflux by potentiometry in the presence of increasing amounts of serum. Both ion currents (Na+ or K+) decreased linearly with the reciprocal of serum amount. The serum effects...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gibot S,Jeminet G,Juillard J,Gumila C,Ancelin ML,Vial H,Delort AM

    更新日期:1999-03-15 00:00:00

  • Factors influencing pyrroline 5-carboxylate synthesis from glutamate by rat intestinal mucosa mitochondria.

    abstract::Factors influencing pyrroline 5-carboxylate (P5C) synthesis from glutamate by a subcellular fraction enriched in mitochondria of rat small intestinal mucosa have been studied. P5C synthesis decreased rapidly if this subcellular fraction was preincubated at 20 degrees C in the absence of substrates; this effect suggest...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Henslee JG,Wakabayashi Y,Small C,Jones ME

    更新日期:1983-10-15 00:00:00

  • Papain hydrolysis of X-phenyl-N-methanesulfonyl glycinates: a quantitative structure-activity relationship and molecular graphics analysis.

    abstract::The hydrolysis of 32 X-phenyl-N-methanesulfonyl glycinates by papain was investigated. It was found that the variation in the Michaelis constants could be rationalized by the following correlation equation: log 1/Km = 0.61 pi '3 + 0.46 MR4 + 0.55 sigma + 2.00 with a correlation coefficient of 0.945. In this expression...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Carotti A,Smith RN,Wong S,Hansch C,Blaney JM,Langridge R

    更新日期:1984-02-15 00:00:00

  • Functional analysis, using in vitro mutagenesis, of amino acids located in the phenylalanine hydroxylase active site.

    abstract::The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kineti...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Jennings IG,Cotton RG,Kobe B

    更新日期:2000-12-15 00:00:00

  • Chromate-induced chromium(V) formation in live mice and its control by cellular antioxidants: an L-band electron paramagnetic resonance study.

    abstract::A recent note from our laboratory reported that L-band (1.2 GHz) electron paramagnetic resonance spectroscopy can be utilized in detecting the formation of Cr(V) intermediates from chromate-treated whole mice. Since Cr(V) is thought to be one of the key species in the mechanism of chromate's toxicity, we carried out a...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liu KJ,Shi X,Jiang JJ,Goda F,Dalal N,Swartz HM

    更新日期:1995-10-20 00:00:00

  • Purification and properties of a mitochondrial NAD+ glycohydrolase.

    abstract::A 60- to 70-fold purification of an NAD+ glycohydrolase from the inner membrane of rat liver mitochondria to apparent homogeneity on sodium dodecyl sulfate (SDS)-polyacrylamide slab gel is described. The minimum molecular weight of the enzyme on polyacrylamide gels in the presence of SDS is around 62,000. The enzyme s...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Moser B,Winterhalter KH,Richter C

    更新日期:1983-07-01 00:00:00

  • Site-specific modification of hemoglobin by methyl acetyl phosphate.

    abstract::Methyl acetyl phosphate, which was originally synthesized as a site-specific reagent for hydroxybutyrate dehydrogenase [R. Kluger and W.-C. Tsui (1980) J. Org. Chem. 45, 2723], also has an affinity for the binding site for 2,3-diphosphoglycerate in hemoglobin. Three residues in or near this cleft between the beta-chai...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ueno H,Pospischil MA,Manning JM,Kluger R

    更新日期:1986-02-01 00:00:00

  • Purification and characterization of an acyclic monoterpene primary alcohol:NADP+ oxidoreductase from catmint (Nepeta racemosa).

    abstract::A soluble monoterpene primary alcohol:NADP+ oxidoreductase has been purified to apparent homogeneity from leaves of the catmint, Nepeta racemosa. The purified enzyme consisted of two polypeptides, with molecular masses of 42,000 and 40,000 Da, and contained zinc ions. A number of monoterpene alcohols (geraniol, nerol,...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hallahan DL,West JM,Wallsgrove RM,Smiley DW,Dawson GW,Pickett JA,Hamilton JG

    更新日期:1995-04-01 00:00:00

  • Characterization of the intracellular and the plasma membrane Ca2+-ATPases in fractionated pig brain membranes using calcium pump inhibitors.

    abstract::The Ca2+-ATPase activity of isolated membranes and purified plasma membrane ATPase from pig brain was measured in the presence of specific inhibitors. The inhibition of the enzymatic activity by vanadate presents a lower affinity in microsomes than in the synaptic plasma membrane vesicles, showing K0.5 of 0.4 and 0.2 ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Salvador JM,Mata AM

    更新日期:1998-03-15 00:00:00

  • High temperature stress increases the expression of wheat leaf ribulose-1,5-bisphosphate carboxylase/oxygenase activase protein.

    abstract::The effect of high temperature stress on the expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase was examined in wheat (Triticum aestivum L.) leaves, which normally possess 46- and 42-kDa activase forms. Heat stress at 38 degrees C significantly reduced total activase mRNA levels compared ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Law RD,Crafts-Brandner SJ

    更新日期:2001-02-15 00:00:00

  • Oxidation mechanism of vitamin E analogue (Trolox C, 6-hydroxy-2,2,5,7,8-pentamethylchroman) and vitamin E by horseradish peroxidase and myoglobin.

    abstract::The oxidation of 6-hydroxy-2,2,5,7,8-pentamethylchroman, Trolox C, and alpha-tocopherol by horseradish peroxidase was examined by stopped-flow and ESR experiments. The catalytic intermediate of horseradish peroxidase during the oxidation of vitamin E analogues and vitamin E was invariably Compound II, and rate constan...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Nakamura M,Hayashi T

    更新日期:1992-12-01 00:00:00

  • General features in the stoichiometry and stability of ionophore A23187-cation complexes in homogeneous solution.

    abstract::Existing literature describing the stoichiometry and stability of complexes between A23187 and divalent cations in solution has been extended to include additional transition series cations, the heavy-metal cations Cd2+ and Pb2+, plus seven lanthanide series trivalent cations. Stability constants of 1:1 complexes betw...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chapman CJ,Puri AK,Taylor RW,Pfeiffer DR

    更新日期:1990-08-15 00:00:00

  • Decreased cytochrome oxidase activity and changes in phospholipids in heart mitochondria from hypothyroid rats.

    abstract::The effect of hypothyroidism on kinetic characteristics of cytochrome oxidase in rat heart mitochondria was studied. Mitochondrial preparations from control and hypothyroid rats had equivalent Km values for cytochrome c, while the maximal activity of the oxidase was significantly decreased (more than 30%) in mitochond...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Paradies G,Ruggiero FM,Dinoi P,Petrosillo G,Quagliariello E

    更新日期:1993-11-15 00:00:00

  • Retinoids inhibit the oxidative modification of protein kinase C induced by oxidant tumor promoters.

    abstract::Recently we reported that oxidant tumor promoters can induce the oxidative modification of protein kinase C (PKC) resulting in either activation or inactivation of the kinase (R. Gopalakrishna and W. B. Anderson, Arch. Biochem. Biophys. 285, 382-387, 1991). Since retinoids previously have been shown to antagonize the ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gundimeda U,Hara SK,Anderson WB,Gopalakrishna R

    更新日期:1993-01-01 00:00:00

  • The A31P missense mutation in cardiac myosin binding protein C alters protein structure but does not cause haploinsufficiency.

    abstract::Mutations in MYBPC3, the gene encoding cardiac myosin binding protein C (cMyBP-C), are a major cause of hypertrophic cardiomyopathy (HCM). While most mutations encode premature stop codons, missense mutations causing single amino acid substitutions are also common. Here we investigated effects of a single proline for ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: van Dijk SJ,Bezold Kooiker K,Mazzalupo S,Yang Y,Kostyukova AS,Mustacich DJ,Hoye ER,Stern JA,Kittleson MD,Harris SP

    更新日期:2016-07-01 00:00:00