当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Effects of fluorescein isothiocyanate on insulin actions in rat adipocytes.

Effects of fluorescein isothiocyanate on insulin actions in rat adipocytes.

Abstract：

:The effects of fluorescein isothiocyanate II (FITC) on the actions of insulin in rat adipocytes were studied. When adipocytes were incubated with FITC at pH 7.4 (2 mM agent, 8 min), the cells were completely deprived of their specific insulin-binding activity and rendered unresponsive to the hormone. The effect of FITC on the insulin-binding activity was milder at pH 9.0, and cAMP phosphodiesterase in cells exposed to FITC at pH 9.0 was maximally stimulated if the insulin concentration was increased to 100 nM. Under identical conditions, however, glucose transport activity was rendered not only less sensitive but also less responsive to the hormone. When FITC was added to cells after insulin at pH 9.0, the glucose transport activity that had been stimulated by the hormone was considerably reduced. This reduction was largely, but not entirely, prevented if the cells were deprived of ATP, suggesting that FITC (a) elicited the ATP-dependent reversal of the hormonal effect and, simultaneously, (b) mildly inhibited the transport activity per se. Western blot assay of GLUT-4 (a major isoform of glucose transporter in adipocytes) indicated that FITC (a) partially blocked insulin-dependent translocation of GLUT-4 from the intracellular site to the plasma membrane while it (b) induced a mild "insulin-like" effect. It is concluded that FITC at pH 9.0 (a) renders both glucose transport and phosphodiesterase activities less insulin sensitive presumably by modifying the cellular hormone receptor and (b) makes glucose transport activity less responsive to insulin presumably by (i) blocking hormone-dependent translocation of glucose transporter and (ii) mildly inhibiting intrinsic glucose transport activity.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Goto Y,Sumida Y,Flanagan JE,Robinson FW,Simpson IA,Cushman SW,Kono T

doi

10.1016/0003-9861(92)90389-e

subject

Has Abstract

pub_date

1992-03-01 00:00:00

pages

224-30

issue

2

eissn

0003-9861

issn

1096-0384

pii

0003-9861(92)90389-E

journal_volume

293

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Phospholipase activities of the P388D1 macrophage-like cell line.

    abstract：:The murine macrophage (M phi) cell line, P388D1, was employed as a source of M phi phospholipases in order to characterize the enzymatic properties and subcellular localization of these enzymes because of their importance for prostaglandin biosynthesis. Phospholipase activity was assessed with dipalmitoylphosphatidylc...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90162-6

    authors： Ross MI,Deems RA,Jesaitis AJ,Dennis EA,Ulevitch RJ

    更新日期：1985-04-01 00:00:00

  • Cysteine 98 in CYP3A4 contributes to conformational integrity required for P450 interaction with CYP reductase.

    abstract：:Previously human cytochrome P450 3A4 was efficiently and specifically photolabeled by the photoaffinity ligand lapachenole. One of the modification sites was identified as cysteine 98 in the B-C loop region of the protein [B. Wen, C.E. Doneanu, C.A. Gartner, A.G. Roberts, W.M. Atkins, S.D. Nelson, Biochemistry 44 (200...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2006.08.003

    authors： Wen B,Lampe JN,Roberts AG,Atkins WM,David Rodrigues A,Nelson SD

    更新日期：2006-10-01 00:00:00

  • Functional significance of C-terminal mobile domain of cardiac troponin I.

    abstract：:Ca2+-regulation of cardiac contractility is mediated through the troponin complex, which comprises three subunits: cTnC, cTnI, and cTnT. As intracellular [Ca2+] increases, cTnI reduces its binding interactions with actin to primarily interact with cTnC, thereby enabling contraction. A portion of this regulatory switch...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2017.09.017

    authors： Bohlooli Ghashghaee N,Tanner BCW,Dong WJ

    更新日期：2017-11-15 00:00:00

  • Modification phenomena of solid-state lignin caused by electron-abstracting oxidative systems.

    abstract：:Oxidative treatments of wood pulp lignin by one-electron-abstracting enzymatic or chemical systems result in modification phenomena which are not fully described in terms of those known from lignin model compound studies. The generation of, e.g., long-lived radicals necessitates nondestructive spectroscopic analysis o...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/s0003-9861(02)00225-4

    authors： Barsberg S

    更新日期：2002-08-01 00:00:00

  • Cloning and expression of rabbit and human brain tryptophan hydroxylase cDNA in Escherichia coli.

    abstract：:Rabbit and human brain tryptophan hydroxylase were cloned and expressed in Escherichia coli. Each of the respective cDNAs, including the complete coding sequence of tryptophan hydroxylase, was obtained by reverse transcription of rabbit or human brain mRNA and subcloned into the expression vector pET-3C. The expressed...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1523

    authors： Tipper JP,Citron BA,Ribeiro P,Kaufman S

    更新日期：1994-12-01 00:00:00

  • Gene structure and expression of the targeting subunit, RGL, of the muscle-specific glycogen-associated type 1 protein phosphatase, PP1G.

    abstract：:The type I phosphatase associated with glycogen, PP1G, plays an important role in glycogen metabolism. PP1G is targeted to glycogen by the R(GL) subunit, which regulates the function of the enzyme. We report the cloning and characterization of the gene as well as the pattern of expression of the R(GL) subunit from mou...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2001.2283

    authors： Lannér C,Suzuki Y,Bi C,Zhang H,Cooper LD,Bowker-Kinley MM,DePaoli-Roach AA

    更新日期：2001-04-01 00:00:00

  • Inhibitory effect of CP-25 on intimal formation and vascular hyperplasia via suppression of GRK2/ERK1/2/EVI1 signaling.

    abstract：:Excessive proliferation, migration and dedifferentiation of vascular smooth muscle cells (VSMCs) are the center of intimal formation during in-stent restenosis and vein graft disease. Paeoniflorin-6'-O-benzene sulfonate (CP-25) is known to suppress inflammation and atherogenesis. However, the potential effect of CP-25...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2020.108601

    authors： Zhang J,Liu Y,Long M,Li J,Zhao W,Su Q

    更新日期：2020-11-15 00:00:00

  • Metabolic impact of overexpression of liver glycogen synthase with serine-to-alanine substitutions in rat primary hepatocytes.

    abstract：:To investigate the effect of elevation of liver glycogen synthase (GYS2) activity on glucose and glycogen metabolism, we performed adenoviral overexpression of the mutant GYS2 with six serine-to-alanine substitutions in rat primary hepatocytes. Cell-free assays demonstrated that the serine-to-alanine substitutions cau...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.08.006

    authors： Kadotani A,Fujimura M,Nakamura T,Ohyama S,Harada N,Maruki H,Tamai Y,Kanatani A,Eiki J,Nagata Y

    更新日期：2007-10-15 00:00:00

  • Papain hydrolysis of X-phenyl-N-methanesulfonyl glycinates: a quantitative structure-activity relationship and molecular graphics analysis.

    abstract：:The hydrolysis of 32 X-phenyl-N-methanesulfonyl glycinates by papain was investigated. It was found that the variation in the Michaelis constants could be rationalized by the following correlation equation: log 1/Km = 0.61 pi '3 + 0.46 MR4 + 0.55 sigma + 2.00 with a correlation coefficient of 0.945. In this expression...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90136-x

    authors： Carotti A,Smith RN,Wong S,Hansch C,Blaney JM,Langridge R

    更新日期：1984-02-15 00:00:00

  • Vitamin K-dependent carboxylase: effect of detergent concentrations, vitamin K status, and added protein precursors on activity.

    abstract：:Activity of the rat liver microsomal vitamin K-dependent carboxylase has been studied at various concentrations of detergent. The activity which could be solubilized by 0.25% Triton X-100 was low but could be greatly increased if vitamin K-deficient rats were given vitamin K a few minutes before they were killed. At h...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90519-2

    authors： Shah DV,Swanson JC,Suttie JW

    更新日期：1983-04-01 00:00:00

  • Liver mitochondrial aldehyde dehydrogenase: in vitro expression, in vitro import, and effect of alcohols on import.

    abstract：:An in vitro expression plasmid (pGRAP) that contained the cDNA coding for the rat mitochondrial aldehyde dehydrogenase precursor was constructed, mRNA was synthesized then translated, and the in vitro synthesized precursor of aldehyde dehydrogenase was used in an in vitro import assay. As expected the 19 amino acid si...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90238-5

    authors： Wang TT,Farrés J,Weiner H

    更新日期：1989-08-01 00:00:00

  • Purification and characterization of chorion peroxidase from Aedes aegypti eggs.

    abstract：:Previous study has shown that a peroxidase is present in the mature eggs of Aedes aegypti mosquitoes, and the enzyme is involved in the formation of a rigid and insoluble chorion by catalyzing chorion protein crosslinking through dityrosine formation. In this study, chorion peroxidase was solubilized from egg chorion ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.1821

    authors： Han Q,Li G,Li J

    更新日期：2000-06-01 00:00:00

  • Rat liver pyruvate kinase: influence of ligands on activity and fructose 1,6-bisphosphate binding.

    abstract：:The ability for various ligands to modulate the binding of fructose 1,6-bisphosphate (Fru-1,6-P2) with purified rat liver pyruvate kinase was examined. Binding of Fru-1,6-P2 with pyruvate kinase exhibits positive cooperativity, with maximum binding of 4 mol Fru-1,6-P2 per enzyme tetramer. The Hill coefficient (nH), an...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90536-8

    authors： Blair JB,Walker RG

    更新日期：1984-07-01 00:00:00

  • One-carbon metabolism in lectin-activated human lymphocytes.

    abstract：:Serine is an essential amino acid for the lectin-mediated transformation of human peripheral blood lymphocytes due to the inability of this cell to synthesize sufficient quantities via either the phosphorylated pathway or by reversal of the serine hydroxymethyltransferase reaction to meet the metabolic demands. The le...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90627-7

    authors： Rowe PB,Sauer D,Fahey D,Craig G,McCairns E

    更新日期：1985-01-01 00:00:00

  • The gene encoding translation initiation factor 3 is highly conserved in gram-negative bacteria.

    abstract：:A 1.1-kb Hp alpha I fragment of the Escherichia coli chromosome containing the gene for translation initiation factor 3 was employed as a probe in heterologous hybridization to chromosomal DNA from a variety of other procaryotes. Positive hybridization was observed to DNA derived from all gram-negative bacteria tested...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90276-5

    authors： Pramanik A,Schwartz I

    更新日期：1984-11-15 00:00:00

  • Metabolism of NG,NG-and NG,N'G-dimethylarginine in rats.

    abstract：:The metabolic fates of NG,NG-and NG,N'G-dimethylarginines in rats were investigated isotopically and novel metabolites, alpha-keto-delta-(N,N-dimethylguanidino)-and alpha-keto-delta-(N,N'-dimethylguanidino)valeric acids and gamma-(N,N-dimethylguanidino)-and gamma-(N,N'-dimethylguanidino)butyric acids were identified. ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90060-9

    authors： Ogawa T,Kimoto M,Watanabe H,Sasaoka K

    更新日期：1987-02-01 00:00:00

  • Expression and characterization of human mitochondrial ferredoxin reductase in Escherichia coli.

    abstract：:Ferredoxin reductase (Fd-reductase) supplies reducing equivalents obtained from NADPH to mitochondrial cytochrome P450 enzymes via the small iron-sulfur protein ferredoxin. Two cDNAs (differing by the presence or absence of an 18-bp insert in the coding region) for the human Fd-reductase were subcloned into a newly co...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90749-m

    authors： Brandt ME,Vickery LE

    更新日期：1992-05-01 00:00:00

  • Extracellular ATP modulates podocyte function through P2Y purinergic receptors and pleiotropic effects on AMPK and cAMP/PKA signaling pathways.

    abstract：:Podocytes and their foot processes interlinked by slit diaphragms, constitute a continuous outermost layer of the glomerular capillary and seem to be crucial for maintaining the integrity of the glomerular filtration barrier. Purinergic signaling is involved in a wide range of physiological processes in the renal syst...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2020.108649

    authors： Szrejder M,Rachubik P,Rogacka D,Audzeyenka I,Rychłowski M,Angielski S,Piwkowska A

    更新日期：2020-11-30 00:00:00

  • Interaction of the high-affinity inhibitor tetrahydro-dUMP with the allosteric enzyme deoxycytidylate aminohydrolase.

    abstract：:Tetrahydro-dUMP, an analog of the putative transition state in aminohydrolysis of deoxycytidine monophosphate (dCMP) inhibits the allosteric enzyme deoxycytidylate aminohydrolase with high affinity. The inhibition is reversible, and its kinetics is consistent with the analog binding at the substrate site only to one a...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1138

    authors： Nucci R,Febbraio F,Piccialli G,de Napoli L,Vaccaro C,Rossi M,Whitehead EP

    更新日期：1994-04-01 00:00:00

  • UV light, beta-carotene and human skin--beneficial and potentially harmful effects.

    abstract：:Solar radiation is one of the most important environmental stress agents for human skin, causing sunburn, premature skin aging, and skin cancer. Beta-carotene is discussed to protect against photooxidative stress and thus prevent skin damage. Though beta-carotene has been successfully used against photosensitivity in ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1006/abbi.2001.2313

    authors： Biesalski HK,Obermueller-Jevic UC

    更新日期：2001-05-01 00:00:00

  • Appearance of thymosin alpha 1 in supernatants of monocytes incubated with prothymosin alpha.

    abstract：:Prothymosin alpha, a polypeptide of 109 to 111 amino acid residues, contains the entire thymosin alpha 1 sequence (residues 1-28) at its amino terminal. Human peripheral blood monocytes incubated with prothymosin alpha release thymosin alpha 1 in the culture supernatants. In addition total RNA is found to increase. Th...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90570-m

    authors： Frillingos S,Seferiadis K,Papanastasiou M,Baxevanis CN,Frangou-Lazaridis M,Economou M,Papamichail M,Tsolas O

    更新日期：1992-07-01 00:00:00

  • Dulaglutide inhibits high glucose- induced endothelial dysfunction and NLRP3 inflammasome activation.

    abstract：:Activation of the NLRP3 inflammasome plays an important role in high glucose- induced endothelial dysfunction in patients with type 2 diabetes mellitus (T2DM). Dulaglutide, a newly developed glucagon-like peptide-1 receptor (GLP-1R) agonist, has been approved for the management of T2DM. In the current study, we aimed ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2019.07.008

    authors： Luo X,Hu Y,He S,Ye Q,Lv Z,Liu J,Chen X

    更新日期：2019-08-15 00:00:00

  • The formation of stable fatty acid substrate complexes in prostaglandin H(2) synthase-1.

    abstract：:We have developed a protocol to purify apo-ovine (o) prostaglandin endoperoxide H(2) synthase-1 (PGHS-1) to homogeneity from ram seminal vesicles. The resulting apo enzyme can then be reconstituted with Co(3+)-protoporphyrin IX instead of Fe(3+)-protoporphyrin IX to produce a native-like, but functionally inert, enzym...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.1906

    authors： Malkowski MG,Theisen MJ,Scharmen A,Garavito RM

    更新日期：2000-08-01 00:00:00

  • Enhancement of cytotoxicity of hydrogen peroxide by hyperthermia in chinese hamster ovary cells: role of antioxidant defenses.

    abstract：:Regional hyperthermia has potential for human cancer treatment, particularly in combination with systemic chemotherapy or radiotherapy. The mechanisms involved in heat-induced cell killing are currently unknown. Hyperthermia may increase oxidative stress in cells, and thus, oxidative stress could have a role in the me...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1998.1087

    authors： Lord-Fontaine S,Averill DA

    更新日期：1999-03-15 00:00:00

  • Inhibition of protein tyrosine phosphatases PTP1B and CD45 by sulfotyrosyl peptides.

    abstract：:Sulfotyrosyl peptides corresponding to the known high-affinity substrate phosphotyrosyl peptide sequences in casein and the autophosphorylation sites of insulin receptor and EGF receptor were investigated as inhibitors of protein tyrosine phosphatases PTP1B and CD45. These peptides inhibit both PTP1B and CD45 in the m...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1998.0691

    authors： Desmarais S,Jia Z,Ramachandran C

    更新日期：1998-06-15 00:00:00

  • Dihydrocapsaicin suppresses proinflammatory cytokines expression by enhancing nuclear factor IA in a NF-κB-dependent manner.

    abstract：BACKGROUND:Atherosclerosis is a chronic inflammatory disease and represents the leading cause of morbidity and mortality throughout the world. Accumulating evidences have showed that Dihydrocapsaicin (DHC) has been found to exert multiple pharmacological and physiological effects. Nevertheless, the effects and possible...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2016.06.002

    authors： Zhao JJ,Hu YW,Huang C,Ma X,Kang CM,Zhang Y,Guo FX,Lu JB,Xiu JC,Qiu YR,Sha YH,Gao JJ,Wang YC,Li P,Xu BM,Zheng L,Wang Q

    更新日期：2016-08-15 00:00:00

  • Reexamination of the carbohydrate binding stoichiometry of lima bean lectin.

    abstract：:The carbohydrate binding stoichiometry of lima bean lectin component III was reexamined using equilibrium dialysis and quantitative affinity chromatography following limited chemical modification. Equilibrium dialysis employing methyl[2-14C]benzamido-2-deoxy-alpha-D-galactopyranoside as ligand demonstrated that the le...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90113-9

    authors： Roberts DD,Goldstein IJ

    更新日期：1984-04-01 00:00:00

  • Purification and some properties of carbonic anhydrase from bovine skeletal muscle.

    abstract：:Procedures for the purification of bovine muscle carbonic anhydrase (isoenzyme III) are described. The purified enzyme has a molecular weight near 29,000 and contains one Zn2+ ion per molecule. The sedimentation coefficient, s(0)20,w, is 2.8 X 10(-13) s, the isoelectric pH is 8.5, and A280(0.1%) = 2.07 cm-1. The CO2 h...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90589-2

    authors： Engberg P,Millqvist E,Pohl G,Lindskog S

    更新日期：1985-09-01 00:00:00

  • The regulatory function of N-terminal AAA+ ATPase domain of eukaryote-like archaeal Orc1/Cdc6 protein during DNA replication initiation.

    abstract：:Archaeal replication machinery represents a core version of this in eukaryotes. The crenarchaeon Sulfolobus solfataricus has the potential to be a powerful model system to understand the central mechanism of eukaryotic DNA replication because it contains three active origins of replication and three eukaryote-like Orc...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.01.007

    authors： He ZG,Feng Y,Wang J,Jiang PX

    更新日期：2008-03-15 00:00:00

  • Mammalian glycosyltransferases prefer glycosyl phosphoryl dolichols rather than glycosyl phosphoryl polyprenols as substrates for oligosaccharyl synthesis.

    abstract：:We have studied the effectiveness of polyprenyl-P-mannose and polyprenol-P-glucose as donor substrates for the dolichyl-P-mannose:Man5(GlcNAc)2-PP-dolichol mannosyltransferase and the dolichyl-P-glucose:Man9(GlcNAc)2-PP-dolichol glucosyltransferase, respectively. The polyprenol moiety differs from dolichol only in the...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1070

    authors： D'Souza-Schorey C,McLachlan KR,Krag SS,Elbein AD

    更新日期：1994-02-01 00:00:00