Purification and characterization of D-myo-inositol (1,4,5)/(1,3,4,5)- polyphosphate 5-phosphatase from skeletal muscle.

abstract：:The cytosol fraction of an extract of Xenopus laevis ovaries contains a protein inhibitor that can specifically block the activation of calmodulin-sensitive cyclic nucleotide phosphodiesterase (PDE I) found in that tissue. This inhibitor was purified by DEAE-cellulose chromatography, gel filtration on Sephacryl S-200,...

journal_title：Archives of biochemistry and biophysics

authors： Jedlicki E,Orellana O,Allende CC,Allende JE

更新日期：1985-08-15 00:00:00

abstract：:The vanadate-stimulated oxidation of NADH by an enzymatic flux of O2- is inhibited by superoxide dismutase, but not by catalase. Keller et al. (1989, Free Radical Biol. Med. 6, 15-22) observed inhibition by catalase presumably because they used a commercial preparation contaminated with superoxide dismutase. Their pro...

journal_title：Archives of biochemistry and biophysics

authors： Liochev SI,Fridovich I

更新日期：1989-11-15 00:00:00

abstract：:Organic acidurias (OADs) are inherited disorders of amino acid metabolism biochemically characterized by accumulation of short-chain carboxylic acids in tissues and biological fluids of the affected patients and clinically by predominant neurological manifestations. Some of these disorders are amenable to treatment, w...

journal_title：Archives of biochemistry and biophysics

authors： Wajner M,Vargas CR,Amaral AU

更新日期：2020-12-15 00:00:00

abstract：:Assembly of the cellulosome, a large, extracellular cellulase complex, depends upon docking of a myriad of enzymatic subunits to homologous receptors, or cohesin domains, arranged in tandem along a noncatalytic scaffolding protein. Docking to the cohesin domains is mediated by a highly conserved domain, dockerin (DS),...

journal_title：Archives of biochemistry and biophysics

authors： Lytle BL,Volkman BF,Westler WM,Wu JH

更新日期：2000-07-15 00:00:00

abstract：:In the absence of added calcium, inhibition of NAD-specific isocitrate dehydrogenase by ATP occurred without ADP (I0.5 = 1.8 mM) and with 0.2 mM ADP3- (I0.5 = 1.0 mM) at subsaturating substrate concentrations at pH 7.4. Inhibition by ATP was competitive with NAD+ in the presence and absence of ADP and was not reversed...

journal_title：Archives of biochemistry and biophysics

authors： Gabriel JL,Milner R,Plaut GW

更新日期：1985-07-01 00:00:00

abstract：:According to differences in mobility on SDS-polyacrylamide gel electrophoresis, calpastatins (inhibitor proteins of the calcium-dependent proteinase calpain) are classified into the tissue type (100-120 kDa) and the erythrocyte type (70 kDa), which lacks the amino-terminal domains (domains L and 1). We investigated th...

journal_title：Archives of biochemistry and biophysics

authors： Takano E,Nosaka T,Lee WJ,Nakamura K,Takahashi T,Funaki M,Okada H,Hatanaka M,Maki M

更新日期：1993-06-01 00:00:00

abstract：:The nature of the purple complex formed upon the addition of octanoyl-CoA to the medium chain acyl-CoA dehydrogenase from pig kidney has been addressed by chemical quenching studies. Previous work, using quenching in 0.1 M KOH, suggested that the dehydrogenation product, trans-2-octenoyl-CoA, was not a participant in ...

journal_title：Archives of biochemistry and biophysics

authors： Lau SM,Thorpe C

更新日期：1988-04-01 00:00:00

abstract：:P-Glycoprotein (Pgp) is an important transport enzyme composed of two homologous domains and transports a wide range of structurally diverse xenobiotics from the cell. Recent studies have indicated that allosteric interactions occur between the nucleotide binding domains and between the substrate binding domains of th...

journal_title：Archives of biochemistry and biophysics

authors： Wang EJ,Casciano CN,Clement RP,Johnson WW

更新日期：2000-11-01 00:00:00

abstract：:The native conformation of bovine retinal arrestin has been characterized by a variety of spectroscopic methods. The purified protein gives rise to a near uv absorption band centered at 279 nm which results from the absorbance of its 14 tyrosine and one tryptophan residue. The extinction coefficient for this absorptio...

journal_title：Archives of biochemistry and biophysics

authors： Kotake S,Hey P,Mirmira RG,Copeland RA

更新日期：1991-02-15 00:00:00

abstract：:Our results indicate that the previously reported decrease in proton release by proteolyzed purple membrane sheets was due merely to the aggregation state of these preparations and not to the loss of the carboxyl-terminal tail. Changes in H+/M412 ratios obtained for purple and white membrane preparations correlate wit...

journal_title：Archives of biochemistry and biophysics

authors： Arrio B,Johannin G,Volfin P,Lefort-Tran M,Packer L,Robinson AE,Hrabeta E

更新日期：1986-04-01 00:00:00

abstract：:The retinal pigment epithelium (RPE)-specific protein RPE65 is the major protein of the RPE microsomal membrane fraction. Though RPE65 lacks transmembrane domains or signal peptide, detergents are required for its maximally effective solubilization in isotonic buffers. However, in 0.75-1.0 M KCl, RPE65 is as soluble w...

journal_title：Archives of biochemistry and biophysics

authors： Tsilou E,Hamel CP,Yu S,Redmond TM

更新日期：1997-10-01 00:00:00

abstract：:Calycosin, a functional phytoestrogen isoflavone isolated from Radix astragali, has been shown to possess multiple pharmacological properties including anti-cancer activity. However, up to now, the anti-cancer effect and the related mechanism of calycosin on cervical cancer (CC) cells have not been explored. It has be...

journal_title：Archives of biochemistry and biophysics

authors： Zhang D,Sun G,Peng L,Tian J,Zhang H

更新日期：2020-09-30 00:00:00

abstract：:We studied the effect of glycoprotein GPIIb/IIIa (integrin alpha IIb beta 3) receptor occupancy by adenosine 5',1-thiotriphosphate (ATP alpha S), a competitive inhibitor of the ADP receptor, by fibrinogen, and by peptides containing the RGD (Arg-Gly-Asp) sequence as RGDW (Arg-Gly-Asp-Trp), RGDS (Arg-Gly-Asp-Ser), or t...

journal_title：Archives of biochemistry and biophysics

authors： Padoin E,Alexandre A,Cavallini L,Polverino de Laureto P,Rao GH,Doni MG

更新日期：1996-09-15 00:00:00

abstract：:This work addresses a correlation between the redox state of pyridine nucleotides and that of sulfhydryl groups of the mitochondrial membranes. Several major observations emerge: (1) Conditions leading to an oxidation of the pyridine nucleotides such as incubation with tert-butyl hydroperoxide or acetoacetate determin...

journal_title：Archives of biochemistry and biophysics

authors： Bindoli A,Callegaro MT,Barzon E,Benetti M,Rigobello MP

更新日期：1997-06-01 00:00:00

abstract：:A uridinediphosphateglucose:thiohydroximate glucosyltransferase (EC 2.4.1.-) has been purified 3700-fold from Brassica napus L. seedlings. The enzyme catalyzes the formation of desulfoglucosinolates by transfer of glucose from UDP-glucose to thiohydroximates and is believed to be the second to last step involved in gl...

journal_title：Archives of biochemistry and biophysics

authors： Reed DW,Davin L,Jain JC,Deluca V,Nelson L,Underhill EW

更新日期：1993-09-01 00:00:00

abstract：:The interaction of reducing sugars with proteins leads to the formation of advanced glycation end products (AGE) and reactive oxidative species (ROS). ROS peroxidise free or membrane included unsaturated fatty acids, leading to generate reactive aldehydes as advanced lipid peroxidation end products (ALE). Aldehydes fr...

journal_title：Archives of biochemistry and biophysics

authors： Nooshi-Nedamani S,Habibi-Rezaei M,Farzadfard A,Moosavi-Movahedi AA

更新日期：2019-06-15 00:00:00

abstract：:Recently, we characterized a surface antigen (Z-1) of guinea pig macrophages by monoclonal anti-Z-1 antibody. The Z-1 antigen consists of two different polypeptide chains; alpha (140 kDa) and beta (95 kDa). This antigen is closely correlated with the phagocytic activity of the cells for zymosan and presumably function...

journal_title：Archives of biochemistry and biophysics

authors： Hazeki K,Tamoto K,Tada M,Mori Y

更新日期：1989-05-01 00:00:00

abstract：:Nitric oxide formation by GH3 pituitary cells is stimulated by depolarizing concentrations of K+ and by the L-channel Ca2+ agonist Bay kappa 8644 in an additive manner that depends on extracellular Ca2+. Ca(2+)-dependent NO formation at 100 microM arginine was inhibited 50% over a 30-min period by 5 microM NG-amino-L-...

journal_title：Archives of biochemistry and biophysics

authors： Cooper GR,Barr A,Wolff DJ

更新日期：1998-09-15 00:00:00

abstract：:The absorption spectra of Ni(II) bound to the core histone tetramer, (H3-H4)2, of chicken erythrocytes in 500 mM NaCl + 100 mM phosphate (pH 7.4) were recorded. A charge transfer band was seen at 317 nm, characteristic of a bond between Ni(II) and the sulfur atom of Cys-110 of histone H3. The conditional affinity cons...

journal_title：Archives of biochemistry and biophysics

authors： Bal W,Karantza V,Moudrianakis EN,Kasprzak KS

更新日期：1999-04-15 00:00:00

abstract：:This study characterizes the structural and functional significance of sulfhydryl residues in human plasma heparin cofactor II (HCII). For quantification of sulfhydryl groups, the extinction coefficient of HCII was redetermined and found to be 0.593 ml mg-1 cm-1 using second-derivative spectroscopy and multicomponent ...

journal_title：Archives of biochemistry and biophysics

authors： Church FC,Meade JB,Pratt CW

更新日期：1987-12-01 00:00:00

abstract：:The G1 glycoprotein of Punta Toro virus, a member of the bunyavirus family, accumulates in the Golgi complex when it is expressed from cloned cDNA. We previously reported that the information necessary for Golgi retention of the G1 protein is located within the transmembrane domain and a portion of the cytoplasmic dom...

journal_title：Archives of biochemistry and biophysics

authors： Matsuoka Y,Chen SY,Holland CE,Compans RW

更新日期：1996-12-01 00:00:00

abstract：:A type II ribosome-inactivating protein (RIP) was isolated from the bark tissue of Japanese elderberry (Sambucus sieboldiana) and named sieboldin-b. Sieboldin-b is a heterodimeric protein consisting of 27- and 33-kDa subunits and showed strong ribosome-inactivating activity in vitro but did not show in vivo toxicity. ...

journal_title：Archives of biochemistry and biophysics

authors： Rojo MA,Yato M,Ishii-Minami N,Minami E,Kaku H,Citores L,Girbés T,Shibuya N

更新日期：1997-04-15 00:00:00

abstract：:Staphylococcus aureus is associated with several clinically significant infections among humans and infections associated with antibiotic-resistant strains are growing in frequency. Antivirulence strategies shift the target of drugs from bacterial growth to the infection process resulting to milder evolutionary pressu...

journal_title：Archives of biochemistry and biophysics

authors： Cueno ME,Imai K

更新日期：2018-05-01 00:00:00

abstract：:In order to characterize cellular retinoic acid-binding protein (CRABP) from chick embryos, we resolved the [3H]RA-binding activity of embryo extracts by Mono Q anion-exchange chromatography. Fractions containing [3H]RA-binding proteins eluted in two peaks at 7 and 10 min with the latter showing 10-fold higher activit...

journal_title：Archives of biochemistry and biophysics

authors： Venepally P,Reddy LG,Sani BP

更新日期：1996-12-15 00:00:00

abstract：:We conducted a study to determine the effect of different doses of a lutein supplement on serum lutein concentration and macular pigment optical density (MPOD). Lutein is one of the major components of human macular pigment. Eighty-seven subjects received daily doses of 5, 10, or 20 mg of lutein, or a placebo, over a ...

journal_title：Archives of biochemistry and biophysics

authors： Bone RA,Landrum JT

更新日期：2010-12-01 00:00:00

abstract：:1-O-Sinapoylglucose:choline sinapoyltransferase ("sinapine synthase") has been purified from immature seeds of Brassica napus by sequential hydroxylapatite absorption, ion-exchange chromatography, and gel filtration. The purified enzyme has an apparent molecular weight of 65 kDa on gel filtration and a subunit structu...

journal_title：Archives of biochemistry and biophysics

authors： Vogt T,Aebershold R,Ellis B

更新日期：1993-02-01 00:00:00

abstract：:The level of phosphorylation and activation of phosphofructokinase by serotonin (5-hydroxytryptamine) was studied in intact liver flukes Fasciola hepatica. The enzyme was immunoprecipitated with antiserum prepared against pure enzyme from the liver flukes. The resuspended immunoprecipitated enzyme retained most of its...

journal_title：Archives of biochemistry and biophysics

authors： Kamemoto ES,Lan L,Mansour TE

更新日期：1989-06-01 00:00:00

abstract：:Exposure of human erythrocytes to filtered cigarette smoke in vitro inhibited their capacity to reduce dehydroascorbic acid (ascorbate recycling activity). Glucose uptake was not affected, implying that dehydroascorbic acid transport was not inhibited by the smoke treatment. The intracellular reduction of cationic nit...

journal_title：Archives of biochemistry and biophysics

authors： Maranzana A,Mehlhorn RJ

更新日期：1998-02-15 00:00:00

abstract：:Reactive oxygen species (ROS) can be generated in experimental shock states through several different mechanisms. We measured ROS production in metabolically active liver mitochondria from rats rendered septic by cecal ligation and puncture. By polarography, the State 4 and State 3 respiration rates of liver mitochond...

journal_title：Archives of biochemistry and biophysics

authors： Taylor DE,Ghio AJ,Piantadosi CA

更新日期：1995-01-10 00:00:00

abstract：:Uridine kinase is the rate-limiting enzyme in the pyrimidine salvage pathway of all mammalian cells. A cDNA for uridine kinase from mouse brain has been isolated, sequenced, and characterized. This is the first report of a complete nucleotide sequence for mammalian uridine kinase. The isolated cDNA is only 95% complet...

journal_title：Archives of biochemistry and biophysics

authors： Ropp PA,Traut TW

更新日期：1996-12-01 00:00:00