Abstract:
:This work addresses a correlation between the redox state of pyridine nucleotides and that of sulfhydryl groups of the mitochondrial membranes. Several major observations emerge: (1) Conditions leading to an oxidation of the pyridine nucleotides such as incubation with tert-butyl hydroperoxide or acetoacetate determine a decrease of total mitochondrial sulfhydryl groups. Glutathione does not follow the same pattern since it decreases in the presence of tert-butyl hydroperoxide but not in the presence of acetoacetate. In addition, only in the presence of tert-butyl hydroperoxide is the decrease of sulfhydryl groups concomitant with a membrane protein polymerization, observed by polyacrylamide gel electrophoresis. (2) Under all conditions tested, the oxidation of sulfhydryl groups is further stimulated by the presence of calcium and phosphate ions. (3) Respiratory substrates, which prevent the swelling of mitochondria, also partially prevent the decrease of sulfhydryl groups.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Bindoli A,Callegaro MT,Barzon E,Benetti M,Rigobello MPdoi
10.1006/abbi.1997.9986subject
Has Abstractpub_date
1997-06-01 00:00:00pages
22-8issue
1eissn
0003-9861issn
1096-0384pii
S0003-9861(97)99986-0journal_volume
342pub_type
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