Elevated cholesterol and decreased sterol carrier protein-2 in peroxisomes from AS-30D hepatoma compared to normal rat liver.


:Peroxisomes were isolated from AS-30D hepatoma and compared to normal rat liver cells for the purpose of investigating the cholesterol accumulation in the hepatoma cells. Cholesterol was found to be approximately 10-fold higher relative to protein in AS-30D peroxisomes as compared to peroxisomes from normal liver. The peroxisomes from the hepatoma cells were found to be more stable; catalase was not released from these peroxisomes during isolation or osmotic shock of the peroxisomal fraction. The elevated cholesterol level may stabilize the peroxisomal membrane. Sterol carrier protein-2 (SCP-2) levels were measured using a radioimmunoassay (RIA), which indicated the highest concentration of SCP-2 to be in peroxisomes. Hepatoma peroxisomes had a lower concentration of SCP-2 (2.5 micrograms/mg) than normal liver peroxisomes (8 micrograms/mg). Approximately half of all SCP-2 detected was found to be soluble in both hepatoma and normal rat liver cells. Immunoblots from both rat liver and AS-30D fractions demonstrated the presence of the 14-kDa form of SCP-2. The liver fractions also had a 57-kDa immunoreactive protein, which was barely detectable in the AS-30D fractions. The low abundance of the high molecular weight form of SCP-2 from hepatoma peroxisomes and the lower amounts of SCP-2 detected in the AS-30D peroxisomes may be related to the accumulation of cholesterol in the cells.


Arch Biochem Biophys


Lyons HT,Kharroubi A,Wolins N,Tenner S,Chanderbhan RF,Fiskum G,Donaldson RP




Has Abstract


1991-03-01 00:00:00














  • Ligand-induced domain movement in pyruvate kinase: structure of the enzyme from rabbit muscle with Mg2+, K+, and L-phospholactate at 2.7 A resolution.

    abstract::The structure of rabbit muscle pyruvate kinase crystallized as a complex with Mg2+, K+, and L-phospholactate (L-P-lactate) has been solved and refined to 2.7 A resolution. The crystals, grown from solutions of polyethylene glycol 8000 at pH 7.5, belong to the space group P2(1) and have unit cell parameters a = 144.4 A...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Larsen TM,Benning MM,Wesenberg GE,Rayment I,Reed GH

    更新日期:1997-09-15 00:00:00

  • In vivo reversal of glutathione deficiency and susceptibility to in vivo dexamethasone-induced apoptosis by N-acetylcysteine and L-2-oxothiazolidine-4-carboxylic acid, but not ascorbic acid, in thymocytes from gamma-glutamyltranspeptidase-deficient knocko

    abstract::Cellular glutathione is released during apoptosis and may play a role in the regulation of the mitochondrial permeability transition pore. The question of whether only cytosolic glutathione is important in apoptosis, or whether mitochondrial glutathione also plays a role, was investigated using gamma-glutamyltranspept...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Will Y,Kaetzel RS,Brown MK,Fraley TS,Reed DJ

    更新日期:2002-01-15 00:00:00

  • The molecular weight and oligomerization of rabbit thrombomodulin as assessed by sedimentation equilibrium.

    abstract::Lubrol-solubilized rabbit thrombomodulin has been examined by equilibrium sedimentation in buffers that include sufficient D2O to make the detergent neutrally buoyant. Data were acquired at rotor speeds from 12,000 to 28,000 rpm from two thrombomodulin preparations, at protein concentrations from 0.01 to 0.07%, and in...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Winnard PT,Esmon CT,Laue TM

    更新日期:1989-02-15 00:00:00

  • Nucleoside triphosphate synthesis catalysed by adenylate kinase is ADP dependent.

    abstract::Adenylate kinase (Adk) that catalyses the synthesis of ADP from ATP and AMP has also been shown to perform an ATP dependent phosphorylation of ribo- and deoxynucleoside diphosphates to their corresponding nucleoside triphosphate; ATP+(d)NDP<-->ADP+(d)NTP. This reaction, suggested to occur by the transfer of the gamma-...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Willemoës M,Kilstrup M

    更新日期:2005-12-15 00:00:00

  • Novel nonpeptidic inhibitors of peptide deformylase.

    abstract::A novel series of nonpeptidic compounds structurally related to the known anticholesteremic thyropropic acid were found to inhibit Escherichia coli peptide deformylase (PDF), with IC50 values in the low-micromolar range. Kinetic analysis of [4-(4-hydroxyphenoxy)-3,5-diiodophenyl]acetic acid reveals competitive inhibit...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Jayasekera MM,Kendall A,Shammas R,Dermyer M,Tomala M,Shapiro MA,Holler TP

    更新日期:2000-09-15 00:00:00

  • Investigation of plasma induced electrical and chemical factors and their contribution processes to plasma gene transfection.

    abstract::This study has been done to know what kind of factors in plasmas and processes on cells induce plasma gene transfection. We evaluated the contribution weight of three groups of the effects and processes, i.e. electrical, chemical and biochemical ones, inducing gene transfection. First, the laser produced plasma (LPP) ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Jinno M,Ikeda Y,Motomura H,Kido Y,Satoh S

    更新日期:2016-09-01 00:00:00

  • Initiation of lipid peroxidation in submitochondrial particles: effect of respiratory inhibitors.

    abstract::Initiation of lipid peroxidation in the inner mitochondrial membrane was investigated using respiratory substrates and inhibitors and various iron chelates. An iron chelate was required for initiation of lipid peroxidation in the presence of either NADH or NADPH. The two nicotinamide nucleotides exhibited different ac...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Glinn M,Ernster L,Lee CP

    更新日期:1991-10-01 00:00:00

  • Magnesium and neoplasia: from carcinogenesis to tumor growth and progression or treatment.

    abstract::Magnesium is involved in a wide range of biochemical reactions that are crucial to cell proliferation, differentiation, angiogenesis, and apoptosis. Changes in magnesium availability have been shown to influence biological responses of immuno-inflammatory cells. Equally plausible seems to be an involvement of magnesiu...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Wolf FI,Maier JA,Nasulewicz A,Feillet-Coudray C,Simonacci M,Mazur A,Cittadini A

    更新日期:2007-02-01 00:00:00

  • Probing the affinity and specificity of yeast alcohol dehydrogenase I for coenzymes.

    abstract::Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (SceADH) binds NAD+ and NADH less tightly and turns over substrates more rapidly than does horse (Equus caballus) liver alcohol dehydrogenase E isoenzyme (EcaADH), and neither enzyme uses NADP efficiently. Amino acid residues in the proposed adenylate binding po...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Fan F,Plapp BV

    更新日期:1999-07-15 00:00:00

  • Cloning and characterization of an S-formylglutathione hydrolase from Arabidopsis thaliana.

    abstract::A cDNA from Arabidopsis thaliana resembling S-formylglutathione hydrolase (SFGH), an enzyme with putative roles in formaldehyde detoxification in animals and microorganisms, has been cloned and expressed in Escherichia coli. The purified recombinant Arabidopsis enzyme (AtSFGH) was a dimer composed of 31-kDa subunits. ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kordic S,Cummins I,Edwards R

    更新日期:2002-03-15 00:00:00

  • New selective inhibitors of cytochromes P450 2B and their application to antimutagenesis of tamoxifen.

    abstract::2-Isopropenyl-2-methyladamantane (2-PMADA) and 3-isopropenyl-3-methyldiamantane (3-PMDIA) showed potent and selective inhibition of cytochrome P450 (CYP) 2B6-mediated reactions with K(i) values of 5.27 and 2.17 microM, respectively. No effect on activities of other human CYP was found even at concentrations 100-fold h...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Stiborová M,Borek-Dohalská L,Hodek P,Mráz J,Frei E

    更新日期:2002-07-01 00:00:00

  • Ion diffusion potentials across mycoplasma membranes determined by a novel method using a carbocyanine dye.

    abstract::The influence of transmembrane ion fluxes on mycoplasma membrane potentials was studied. Electric membrane potential was calibrated vs fluorescence intensity of a potential-sensitive carbocyanine dye according to delta psi = (RT/F) X log([aIN(1 - IN) - b]/Kint), where IN = I/I0, I0 = maximal fluorescence intensity (ob...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Schummer U,Schiefer HG

    更新日期:1986-02-01 00:00:00

  • Human fatty acid omega-hydroxylase, CYP4A11: determination of complete genomic sequence and characterization of purified recombinant protein.

    abstract::The gene of the human fatty acid omega-hydroxylase, CYP4A11, has been isolated from a human BAC library, and its complete genomic sequence has been determined. The CYP4A11 gene spanned 12,568 bp and contained 12 exons. The known PPAR recognition elements (PPRE), which were reported to be involved in the induction of C...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kawashima H,Naganuma T,Kusunose E,Kono T,Yasumoto R,Sugimura K,Kishimoto T

    更新日期:2000-06-15 00:00:00

  • Modulation of the lipid composition of boar sperm plasma membranes during an acrosome reaction in vitro.

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    journal_title:Archives of biochemistry and biophysics

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    authors: Nikolopoulou M,Soucek DA,Vary JC

    更新日期:1986-10-01 00:00:00

  • Application of a thermodynamic model to the prediction of phase separations in freeze-concentrated formulations for protein lyophilization.

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Heller MC,Carpenter JF,Randolph TW

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  • Tobacco proliferating cell nuclear antigen binds directly and stimulates both activity and processivity of ddNTP-sensitive mungbean DNA polymerase.

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    authors: Roy S,Roy Choudhury S,Mukherjee SK,Sengupta DN

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    authors: Matsuo Y,Nishinaka Y,Suzuki S,Kojima M,Kizaka-Kondoh S,Kondo N,Son A,Sakakura-Nishiyama J,Yamaguchi Y,Masutani H,Ishii Y,Yodoi J

    更新日期:2004-03-01 00:00:00

  • On the mechanism of action of free purine bases on DNA synthesis in serum-starved L-cells treated with platelet extract.

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Flodgaard H,Klenow H

    更新日期:1985-06-01 00:00:00

  • Effect of training on H(2)O(2) release by mitochondria from rat skeletal muscle.

    abstract::In this study, oxygen consumption and H(2)O(2) release rate by succinate or pyruvate/malate supplemented mitochondria isolated from skeletal muscle of trained and untrained rats were investigated. The overall mitochondrial antioxidant capacity and the effect of preincubation of mitochondria with GDP, an inhibitor of u...

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    pub_type: 杂志文章


    authors: Venditti P,Masullo P,Di Meo S

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  • Heterologous expression and characterization of an active lignin peroxidase from Phanerochaete chrysosporium using recombinant baculovirus.

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    authors: Johnson TM,Li JK

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  • Purification and characterization of a vanadate-sensitive nucleotide tri- and diphosphatase with acid pH optimum from rat bone.

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Andersson G,Ek-Rylander B,Hammarström L

    更新日期:1984-02-01 00:00:00

  • Hydroxyl radicals is not a significant intermediate in the vanadate-stimulated oxidation of NAD(P)H by O2.

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liochev SI,Fridovich I

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  • 4-Methylumbelliferyl-glycosides as fluorescence probes of sugar-binding sites on lectin molecules: spectral properties and dependence of fluorescence on polarity and viscosity.

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Decastel M,Vincent M,Matta KL,Frénoy JP

    更新日期:1984-08-01 00:00:00

  • Characterization of tryptic hydrolysis of alpha-lactalbumin/saponin mixture and structural change of alpha-lactalbumin interacting with soybean saponin.

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Shimoyamada M,Okada Y,Watanabe K,Yamauchi R

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Cook NM,Shinyashiki M,Jackson MI,Leal FA,Fukuto JM

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Orr WC,Sohal RS

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zhang S,Sanyal I,Bulboaca GH,Rich A,Flint DH

    更新日期:1994-02-15 00:00:00

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lass A,Sohal RS

    更新日期:1998-04-15 00:00:00

  • Calmodulin-dependent NAD kinase of human neutrophils.

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Williams MB,Jones HP

    更新日期:1985-02-15 00:00:00

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    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Yin JJ,Smith MJ,Eppley RM,Troy AL,Page SW,Sphon JA

    更新日期:1996-11-01 00:00:00