Static compression is associated with decreased diffusivity of dextrans in cartilage explants.

abstract：:Previously human cytochrome P450 3A4 was efficiently and specifically photolabeled by the photoaffinity ligand lapachenole. One of the modification sites was identified as cysteine 98 in the B-C loop region of the protein [B. Wen, C.E. Doneanu, C.A. Gartner, A.G. Roberts, W.M. Atkins, S.D. Nelson, Biochemistry 44 (200...

journal_title：Archives of biochemistry and biophysics

authors： Wen B,Lampe JN,Roberts AG,Atkins WM,David Rodrigues A,Nelson SD

更新日期：2006-10-01 00:00:00

abstract：:Maturation of the precursor forms of bovine cholesterol side-chain cleavage cytochrome P-450 (P-450SCC) and 11 beta-hydroxylase cytochrome P-450 (P-450(11)beta) was investigated using mitochondria from bovine corpus luteum. The results show that both precursors, whose synthesis was directed by bovine adrenocortical RN...

journal_title：Archives of biochemistry and biophysics

authors： Matocha MF,Waterman MR

更新日期：1986-11-01 00:00:00

abstract：:This study showcases the potential of unnatural amino acids to enable non-natural functions when incorporated in the protein scaffold of heme metalloproteins. For this purpose, a genetically-engineered myoglobin (Mb) mutant was created by incorporating redox-active 3-amino-l-tyrosine (NH2Tyr) into its active site, rep...

journal_title：Archives of biochemistry and biophysics

authors： Chand S,Ray S,Wanigasekara E,Yadav P,Crawford JA,Armstrong DW,Rajeshwar K,Pierce BS

更新日期：2018-02-01 00:00:00

abstract：:Cold plasma is partly ionized non-thermal plasma generated at atmospheric pressure. It has been recognized as an alternative approach in medicine for sterilization of wounds, promotion of wound healing, topical treatment of skin diseases with microbial involvement and treatment of cancer. Cold plasma used in wound the...

journal_title：Archives of biochemistry and biophysics

authors： Mohd Nasir N,Lee BK,Yap SS,Thong KL,Yap SL

更新日期：2016-09-01 00:00:00

abstract：:The tertiary structure model of EF-Tu predicts that the amino acid sequence Val-Asp-His-Gly-Lys-Thr-Thr-Leu (residues 20-27) forms a pocket that binds the pyrophosphate group. To test this model we used site-directed mutagenesis to produce forms of EF-Tu altered in this region. The following mutations were constructed...

journal_title：Archives of biochemistry and biophysics

authors： Hwang YW,McCabe PG,Innis MA,Miller DL

更新日期：1989-11-01 00:00:00

abstract：:Csk is a protein tyrosine kinase that phosphorylates other protein tyrosine kinases of the Src family and down-regulates their activities. It is not known how Csk is regulated. We investigated the possibility that Csk is regulated through phosphorylation by examining if Csk can serve as an in vitro substrate for a pan...

journal_title：Archives of biochemistry and biophysics

authors： Sun G,Ke S,Budde RJ

更新日期：1997-07-15 00:00:00

abstract：:Peroxynitrite (PN), the product of the diffusion-limited reaction between nitric oxide (*NO) and superoxide (O*-(2)), represents a relevant mediator of oxidative modifications in low-density lipoprotein (LDL). This work shows for the first time the simultaneous action of low-controlled fluxes of PN and *NO on LDL oxid...

journal_title：Archives of biochemistry and biophysics

authors： Trostchansky A,Batthyány C,Botti H,Radi R,Denicola A,Rubbo H

更新日期：2001-11-15 00:00:00

abstract：:Bovine kidney mitochondrial extracts contain an inactive protamine kinase and an inactive casein kinase. The protamine kinase was activated by chromatography on poly(L-lysine)-agarose. Two forms of this soluble mitochondrial protamine kinase were separated by chromatography on protamine-agarose. Both forms were purifi...

journal_title：Archives of biochemistry and biophysics

authors： Damuni Z,Reed LJ

更新日期：1988-05-01 00:00:00

abstract：:The plasma membrane of cells contains enzymes whose active sites face the external medium rather than the cytoplasm. The activities of these enzymes, referred to as ectoenzymes, can be measured using living cells. In this work we describe the ability of living promastigotes of Leishmania amazonensis to hydrolyze extra...

journal_title：Archives of biochemistry and biophysics

authors： Berrêdo-Pinho M,Peres-Sampaio CE,Chrispim PP,Belmont-Firpo R,Lemos AP,Martiny A,Vannier-Santos MA,Meyer-Fernandes JR

更新日期：2001-07-01 00:00:00

abstract：:Oligopeptidase A (OpdA) belongs to the M3A subfamily of bacterial peptidases with catalytic and structural properties similar to mammalian thimet-oligopeptidase (TOP) and neurolysin (NEL). The three enzymes have four conserved Tyr residues on a flexible loop in close proximity to the catalytic site. In OpdA, the flexi...

journal_title：Archives of biochemistry and biophysics

authors： Lorenzon RZ,Cunha CE,Marcondes MF,Machado MF,Juliano MA,Oliveira V,Travassos LR,Paschoalin T,Carmona AK

更新日期：2010-08-15 00:00:00

abstract：:The study aimed to evaluate the impact of adenosine receptors (ARs) on human colon tumor cells (HCT 116, HT-29) growth and sensitivity to 5-Fluorouracil (5-FU) an anticancer chemotherapeutic drug. The exposure of cancer cells to a selective A(3)-AR agonist (IB-MECA) resulted in an increase in HT-29 cells number, where...

journal_title：Archives of biochemistry and biophysics

authors： Sakowicz-Burkiewicz M,Kitowska A,Grden M,Maciejewska I,Szutowicz A,Pawelczyk T

更新日期：2013-05-01 00:00:00

abstract：:The heme enzyme lignin peroxidase contains a unique Cbeta-hydroxylated tryptophan residue (Trp171) on the surface of the enzyme. Mutagenetic substitution of Trp171 abolishes completely the veratryl alcohol oxidation activity of the enzyme. This led us to surmise that Trp171 may be involved in electron transfer from na...

journal_title：Archives of biochemistry and biophysics

authors： Blodig W,Smith AT,Winterhalter K,Piontek K

更新日期：1999-10-01 00:00:00

abstract：:We have previously demonstrated that dietary fat, especially unsaturated fatty acids, induces cellular retinol-binding protein, type II (CRBPII) gene expression in rat jejunum. In the present study, we showed that feeding a high-fat diet caused parallel increases in jejunal CRBPII mRNA and CRBPII pre-mRNA levels. Nucl...

journal_title：Archives of biochemistry and biophysics

authors： Suruga K,Mochizuki K,Kitagawa M,Goda T,Horie N,Takeishi K,Takase S

更新日期：1999-02-01 00:00:00

abstract：:Affinity columns prepared by immobilizing monoclonal antibodies that specifically recognize the Lea or the Leb blood group antigens can be used for analytical or preparative isolation of oligosaccharides with the corresponding reactivities. The number of immobilized functional antibody combining sites on a column and ...

journal_title：Archives of biochemistry and biophysics

authors： Dakour J,Lundblad A,Zopf D

更新日期：1988-07-01 00:00:00

abstract：:A large portion of mitochondrial asparagine (Asn) is degraded by asparagine amino-transferase to produce alpha-ketosuccinamate (alpha KSA), which is then hydrolized by omega-amidase to produce oxaloacetate (OAA) and ammonia. This is in contrast to the catabolism in the cytosol, where the main catabolic route for Asn o...

journal_title：Archives of biochemistry and biophysics

authors： Moraga-Amador DA,MacPhee-Quiggley KM,Keefer JF,Schuster SM

更新日期：1989-01-01 00:00:00

abstract：:The glucose transporter of the bacterial phosphotransferase system mediates sugar transport across the cytoplasmic membrane concomitant with sugar phosphorylation. It consists of a cytoplasmic subunit IIA(Glc) and the transmembrane subunit IICB(Glc). IICB(Glc) was purified to homogeneity by urea/alkali washing of memb...

journal_title：Archives of biochemistry and biophysics

authors： Zhuang J,Gutknecht R,Flükiger K,Hasler L,Erni B,Engel A

更新日期：1999-12-01 00:00:00

abstract：:Cellular glutathione is released during apoptosis and may play a role in the regulation of the mitochondrial permeability transition pore. The question of whether only cytosolic glutathione is important in apoptosis, or whether mitochondrial glutathione also plays a role, was investigated using gamma-glutamyltranspept...

journal_title：Archives of biochemistry and biophysics

authors： Will Y,Kaetzel RS,Brown MK,Fraley TS,Reed DJ

更新日期：2002-01-15 00:00:00

abstract：:Hippocampal-dependent cognitive functions rely on production of new neurons and maintenance of dendritic structures to provide the synaptic plasticity needed for learning and formation of new memories. Hippocampal formation is exquisitely sensitive to patho-physiological changes, and reduced antioxidant capacity and e...

journal_title：Archives of biochemistry and biophysics

authors： Huang TT,Leu D,Zou Y

更新日期：2015-06-15 00:00:00

abstract：:M. Kuwajima, C. B. Newgard, D. W. Foster, and J. D. McGarry (1986, J. Biol. Chem. 261, 8849-8853) have concluded that the reason postprandial hepatic glycogenesis occurs primarily from gluconeogenic precursors rather than glucose is because glucokinase activity is insufficient to support the observed rates of glycogen...

journal_title：Archives of biochemistry and biophysics

authors： Davidson AL,Arion WJ

更新日期：1987-02-15 00:00:00

abstract：:This work addresses a correlation between the redox state of pyridine nucleotides and that of sulfhydryl groups of the mitochondrial membranes. Several major observations emerge: (1) Conditions leading to an oxidation of the pyridine nucleotides such as incubation with tert-butyl hydroperoxide or acetoacetate determin...

journal_title：Archives of biochemistry and biophysics

authors： Bindoli A,Callegaro MT,Barzon E,Benetti M,Rigobello MP

更新日期：1997-06-01 00:00:00

abstract：:Using fractionation procedures such as sucrose gradient sedimentation and gel permeation chromatography, a novel cytosolic binding protein for benzo[a]pyrene has been detected in liver of nonmammalian and mammalian species including human. This protein, called 8 S protein, cosediments with the Ah receptor after centri...

journal_title：Archives of biochemistry and biophysics

authors： Lesca P,Peryt B,Soues S,Maurel P,Cravedi JP

更新日期：1993-05-15 00:00:00

abstract：:Recombinant human lymphotoxin (rhLT) produced by CHO cells transfected with human LT genomic DNA was purified to homogeneity, but approximately 5% of the molecules were devoid of the last two amino terminal residues. A peptide N-glycosylated at Asn62 (Tr-45) and one partially O-glycosylated at Thr7 (Tr-14) on cleavage...

journal_title：Archives of biochemistry and biophysics

authors： Fukushima K,Watanabe H,Takeo K,Nomura M,Asahi T,Yamashita K

更新日期：1993-07-01 00:00:00

abstract：:In this study, we have analyzed interflavin electron transfer reactions from FAD to FMN in both the full-length inducible nitric oxide synthase (iNOS) and its reductase domain. Comparison is made with the interflavin electron transfer in NADPH-cytochrome P450 reductase (CPR). For the analysis of interflavin electron t...

journal_title：Archives of biochemistry and biophysics

authors： Yamamoto K,Kimura S,Shiro Y,Iyanagi T

更新日期：2005-08-01 00:00:00

abstract：:A green manganese desferrioxamine complex is rapidly formed at room temperature upon stirring freshly precipitated manganese dioxide in a solution of the ligand. Spectral studies and low-temperature ESR indicate that this compound, which has been previously described as a manganese(III) complex, is better characterize...

journal_title：Archives of biochemistry and biophysics

authors： Rush JD,Maskos Z,Koppenol WH

更新日期：1991-08-15 00:00:00

abstract：:Salicylhydroxamic acid reductase, which catalyzes the reduction of salicylhydroxamic acid to salicylamide, was purified from rat liver cytosol. The purification procedure consisted of fractionation with ammonium sulfate, chromatography with Phenyl-Toyopearl 650, DEAE-cellulose, hydroxyapatite, and Sephadex G-200, and ...

journal_title：Archives of biochemistry and biophysics

authors： Katsura H,Kitamura S,Tatsumi K

更新日期：1993-05-01 00:00:00

abstract：:Hepatocytes from fed rats were incubated for 120 min in the presence of alpha-D-[1,2-13C]glucose pentaacetate (1.7 mM), both D-[1,2-13C]glucose (1.7 mM) and acetate (8.5 mM), alpha-D-glucose penta[2-13C]acetate (1.7 mM), or D-[1,2-13C]glucose (8.3 mM). The amounts of 13C-enriched L-lactate and D-glucose and those of a...

journal_title：Archives of biochemistry and biophysics

authors： Malaisse WJ,Ladrière L,Kadiata MM,Verbruggen I,Willem R

更新日期：2000-09-01 00:00:00

abstract：:The major phenobarbital-inducible form of cytochrome P-450 (cytochrome P-450 PB) was purified to homogeneity from rat liver microsomes and rabbit antibodies prepared against the purified enzyme. Using these antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed for the detection of cytochrome P-450 PB ...

journal_title：Archives of biochemistry and biophysics

authors： Seidel SL,Shawver LK,Shires TK

更新日期：1984-03-01 00:00:00

abstract：:In this study, the binding points of MethB and two structurally-related cationic phenoxazine dyes [meldola blue (MB) and nile blue (NB)] to human butyrylcholinesterase (BChE) were investigated by molecular docking and site directed mutagenesis. The comparative inhibitory effects of MethB, MB and NB on recombinant wild...

journal_title：Archives of biochemistry and biophysics

authors： Sezgin Z,Biberoglu K,Chupakhin V,Makhaeva GF,Tacal O

更新日期：2013-04-01 00:00:00

abstract：:Glucocorticoid (GCs) hormones exert their actions via their cognate steroid receptors the Glucocorticoid Receptors (GR), by genomic or non-genomic mechanisms of actions. GCs regulate many cellular functions among them growth, metabolism, immune response and apoptosis. Due to their cell type specific induction of apopt...

journal_title：Archives of biochemistry and biophysics

authors： Karra AG,Tziortziou M,Kylindri P,Georgatza D,Gorgogietas VA,Makiou A,Krokida A,Tsialtas I,Kalousi FD,Papadopoulos GE,Papadopoulou KΚ,Psarra AG

更新日期：2020-11-30 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) stimulates angiogenesis. Human hepatocellular carcinoma (HCC) is a VEGF-driven tumor often associated with chronic hepatitis B or C virus infection. The woodchuck is a well-characterized model of hepatitis B virus related HCC and a valuable tool for translational studies of no...

journal_title：Archives of biochemistry and biophysics

authors： Huang H,Salavaggione O,Rivera L,Mukherjee S,Brekken R,Tennant B,Iyer R,Adjei A

更新日期：2019-01-01 00:00:00