Import and processing of P-450SCC and P-450(11) beta precursors by corpus luteal mitochondria: a processing pathway recognizing homologous and heterologous precursors.

abstract：:Intrinsically disordered proteins represent about one third of eukaryotic proteins. An additional third correspond to proteins containing folded domains as well as large intrinsically disordered regions (IDR). While IDRs may represent functionally autonomous domains, in some instances it has become clear that they pro...

journal_title：Archives of biochemistry and biophysics

authors： Arbesú M,Pons M

更新日期：2019-11-30 00:00:00

abstract：:NAD kinase from human neutrophils has been partially purified by sequential application of Red Agarose, ion-exchange, and gel-filtration chromatography. The enzyme has a broad pH optimum, 7.0-9.5, is strictly dependent upon the presence of Mg2+, and in the absence of calcium exhibits Km values of 0.6 and 0.9 mM for NA...

journal_title：Archives of biochemistry and biophysics

authors： Williams MB,Jones HP

更新日期：1985-02-15 00:00:00

abstract：:In previous studies [FEBS Lett. 434 (1998) 231, Arch. Biochem. Biophys. 404 (2002) 92], we demonstrated for the first time that D-aspartate (D-Asp) is synthesized in cultured mammalian cell lines, such as pheochromocytoma 12 (PC12) and its subclone, MPT1. Our current focus is analysis of the dynamics of D-Asp homeosta...

journal_title：Archives of biochemistry and biophysics

authors： Adachi M,Koyama H,Long Z,Sekine M,Furuchi T,Imai K,Nimura N,Shimamoto K,Nakajima T,Homma H

更新日期：2004-04-01 00:00:00

abstract：:The purpose of this review is to summarise the current state of knowledge of (i) the kinetics and mechanisms of radical reactions with carotenoids, (ii) the properties of carotenoid radicals, and (iii) the antioxidant/pro-oxidant properties of carotenoids. ...

journal_title：Archives of biochemistry and biophysics

authors： El-Agamey A,Lowe GM,McGarvey DJ,Mortensen A,Phillip DM,Truscott TG,Young AJ

更新日期：2004-10-01 00:00:00

abstract：:Two new types of bacterial Fe-S L-serine dehydratases have been identified. These join two previously recognized enzyme types, for a total of four, that are distinguished on the basis of domain arrangement and amino acid sequence. A Type 3 enzyme from Amphibacillus xylanus (axLSD) and a Type 4 enzyme from Heliscomenob...

journal_title：Archives of biochemistry and biophysics

authors： Xu XL,Grant GA

更新日期：2013-12-01 00:00:00

abstract：:The human red blood cell (RBC) membrane has significant elastic capabilities which can be described measuring typical membrane edge fluctuations and mechanical properties by optical techniques. The RBC elastic properties can be affected by changes in the surrounding media. In an attempt to elucidate the molecular mech...

journal_title：Archives of biochemistry and biophysics

authors： Gallardo MJ,Suwalsky M,Ramírez D,Tapia J,Sepulveda B

更新日期：2019-04-15 00:00:00

abstract：:Chlorophyllase catalyzes the initial step in the degradation of chlorophyll and plays a key role in leaf senescence and fruit ripening. Here, we report the cloning of chlorophyllase from Triticum aestivum (wheat) and provide a detailed mechanistic analysis of the enzyme. Purification of recombinant chlorophyllase from...

journal_title：Archives of biochemistry and biophysics

authors： Arkus KA,Cahoon EB,Jez JM

更新日期：2005-06-15 00:00:00

abstract：:No larger molecular weight component appeared in the high-performance gel chromatogram when calcium alone was added to the riboflavin-binding protein solution (RfBP), indicating that calcium alone did not aggregate it. RfBP bound calcium, but the amount of bound calcium decreased markedly upon dephosphorylation. Cross...

journal_title：Archives of biochemistry and biophysics

authors： Aoki T,Yamao Y,Yonemasu E,Kumasaki Y,Kako Y

更新日期：1993-09-01 00:00:00

abstract：:An in vitro assay that measures endosome fusion was used to characterize the role of guanosine triphosphate (GTP)-binding proteins in endocytosis. Guanosine 5',3-(thio)triphosphate (GTP gamma S), a nonhydrolyzable analog of GTP, stimulates the binding of cytosolic factors to the endosomal membrane (priming). GTP gamma...

journal_title：Archives of biochemistry and biophysics

authors： Lenhard JM,Colombo MI,Stahl PD

更新日期：1994-08-01 00:00:00

abstract：:Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase [ATP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.49] is completely inactivated by the 2',3'-dialdehyde derivative of ATP (oATP) in the presence of Mn2+. The dependence of the pseudo-first-order rate constant on reagent concentration indicates t...

journal_title：Archives of biochemistry and biophysics

authors： Saavedra C,Araneda S,Cardemil E

更新日期：1988-11-15 00:00:00

abstract：:Osmolytes are small organic molecules accumulated by organisms under stress conditions to protect macromolecular structure and function. In the present study, we have investigated the effect of several binary osmolyte mixtures on the protein folding/stability and function of RNase-A. For this, we have measured ΔGD(o) ...

journal_title：Archives of biochemistry and biophysics

authors： Warepam M,Singh LR

更新日期：2015-05-01 00:00:00

abstract：:The effects of racemic (+/-) and (+)- and (-)-stereoisomers of isoflurane on depolarization-induced (45)Ca(2+) fluxes mediated by voltage-dependent Ca(2+) channels were investigated in transverse tubule membrane vesicles from rabbit skeletal muscle. In the concentration range 0.5 to 2 mM, (+/-)-isoflurane inhibited (4...

journal_title：Archives of biochemistry and biophysics

authors： Oz M,Tchugunova Y,Dinc M,Dunn SM

更新日期：2002-02-15 00:00:00

abstract：:Hepatic flavin-containing monooxygenases catalyze NADPH-dependent oxygenation of a wide variety of drugs that possess a nucleophilic heteroatom. Two forms of these enzymes (form 1 and 2) have been isolated from rabbit liver microsomes and partially characterized (Ozols, J., 1989, Biochem. Biophys. Res. Commun. 163, 49...

journal_title：Archives of biochemistry and biophysics

authors： Ozols J

更新日期：1991-10-01 00:00:00

abstract：:Studies in vascular smooth muscle cells suggest that, angiotensin II (Ang II)-mediated cellular response requires transactivation of epidermal growth factor receptor (EGF-R), and involves tyrosine phosphorylation of caveolin-1. Here we demonstrate that, exposure of WB rat liver cells to Ang II does not cause transacti...

journal_title：Archives of biochemistry and biophysics

authors： Krishna SB,Alfonso LF,Thekkumkara TJ,Abbruscato TJ,Bhat GJ

更新日期：2007-01-01 00:00:00

abstract：:A marine snail digestive phospholipase A2 (mSDPL) was purified from delipidated hepatopancreas. Unlike known digestive phospholipases A2, which are 14 kDa proteins, the purified mSDPL has a molecular mass of about 30 kDa. It has a specific activity of about 180 U/mg measured at 50 degrees C and pH 8.5 using phosphatid...

journal_title：Archives of biochemistry and biophysics

authors： Zarai Z,Bacha AB,Horchani H,Bezzine S,Zouari N,Gargouri Y,Mejdoub H

更新日期：2010-02-15 00:00:00

abstract：:A theoretical and experimental study has been made of the advancing elution profile in frontal gel chromatography of interacting systems for which the elution volume of the complex is smaller than that of the larger reactant. First, Gilbert-Jenkins theory is used to delineate the form of the elution profile from the m...

journal_title：Archives of biochemistry and biophysics

authors： Cann JR,Winzor DJ

更新日期：1987-07-01 00:00:00

abstract：:A number of natural or synthetic compounds as AGE inhibitors have been proposed, discovered or currently being advanced by others and us. We have identified two new classes of aromatic compounds; aryl- (and heterocyclic) ureido and aryl (and heterocyclic) carboxamido phenoxyisobutyric acids, and benzoic acid derivativ...

journal_title：Archives of biochemistry and biophysics

authors： Rahbar S,Figarola JL

更新日期：2003-11-01 00:00:00

abstract：:The ionization of 4-nitroimidazole to 4-nitroimidazolate was investigated as a function of ionic strength. The apparent pKa varies from 8.99 to 9.50 between 0.001 and 1.0 M ionic strength, respectively, at 25 degrees C. The ionic strength dependence of this ionization is anomalous. The binding of 4-nitroimidazole by h...

journal_title：Archives of biochemistry and biophysics

authors： Taylor KC,Vitello LB,Erman JE

更新日期：2000-10-15 00:00:00

abstract：:Targeting of protein kinase A (PKA) by A-kinase anchoring proteins (AKAPs) contributes to high specificity of PKA signaling pathways. PKA phosphorylation of myofilament and cytoskeletal proteins may regulate myofibrillogenesis and myocyte remodeling during heart disease; however, known cardiac AKAPs do not localize to...

journal_title：Archives of biochemistry and biophysics

authors： Russell MA,Lund LM,Haber R,McKeegan K,Cianciola N,Bond M

更新日期：2006-12-15 00:00:00

abstract：:Cardiac hypertrophy is an adaptive response to hemodynamic stress to compensate for cardiac dysfunction. MicroRNAs can regulate cardiac function and play a vital role in the regulation of cardiac hypertrophy. In the current study, in vivo and vitro hypertrophy models are established to explore the role of miR-27b and ...

journal_title：Archives of biochemistry and biophysics

authors： Zhang M,Cheng K,Chen H,Tu J,Shen Y,Pang L,Wu W

更新日期：2020-08-15 00:00:00

abstract：:Previously, we reported that emptying of intracellular Ca(2+) pools with endoplasmatic Ca(2+)-ATP-ase inhibitor thapsigargin leads to the Na(+) influx in human lymphocytes (M. Tepel et al., 1994, J. Biol. Chem. 269, 26239-26242). In the present study we examined the mechanism underlying the thapsigargin-induced Na(+) ...

journal_title：Archives of biochemistry and biophysics

authors： Nofer JR,Junker R,Walter M,Seedorf U,Assmann G,Zidek W,Tepel M

更新日期：2000-02-15 00:00:00

abstract：:The Ca2+-ATPase activity of isolated membranes and purified plasma membrane ATPase from pig brain was measured in the presence of specific inhibitors. The inhibition of the enzymatic activity by vanadate presents a lower affinity in microsomes than in the synaptic plasma membrane vesicles, showing K0.5 of 0.4 and 0.2 ...

journal_title：Archives of biochemistry and biophysics

authors： Salvador JM,Mata AM

更新日期：1998-03-15 00:00:00

abstract：:The effect of CAM [cyclic AMP, Mg-ATP, and 3-isobutyl, 1-methylxanthine (MIX)] on triacylglycerol (TG) lipase activity in extracts from heparin-perfused rat heart was determined. TG lipase activity in homogenate, 10,000g supernatant, 105,000g supernatant, ammonium sulfate supernatant, and the eluate from heparin-Sepha...

journal_title：Archives of biochemistry and biophysics

authors： Palmer WK,Caruso RA,Oscai LB

更新日期：1986-09-01 00:00:00

abstract：:The sensitivity of animals to endotoxin differs significantly between species. Thus, factors that determine the susceptibility to endotoxin may play important roles in the pathogenesis of septic shock. In order to determine the mechanism responsible for susceptibility to endotoxin, the effect of lipopolysaccharide (LP...

journal_title：Archives of biochemistry and biophysics

authors： Kida T,Kuroki T,Kobayashi K,Inoue M

更新日期：1994-07-01 00:00:00

abstract：:DLIF (digoxin-like immunoreactive factor) and OLF (ouabain-like factor) are endogenous steroid-like ligands (approximately 781 and 595 Da, respectively) with molecular and structural properties similar to the plant-derived cardiac glycosides, digoxin and ouabain. We developed a purification method with a sufficiently ...

journal_title：Archives of biochemistry and biophysics

authors： Qazzaz HM,Valdes R Jr

更新日期：1996-04-01 00:00:00

abstract：:A thymidine-diphospho-4-keto-6-deoxy-D-glucose epimerase was purified from Saccharopolyspora erythraea, the producer of the macrolide antibiotic erythromycin, by a high resolution chromatographic method that exploited the difference in behavior of the protein on ion exchange columns at pH 7.5 and 5.5. By this procedur...

journal_title：Archives of biochemistry and biophysics

authors： Jarvis BW,Hutchinson CR

更新日期：1994-01-01 00:00:00

abstract：:This work reports the first functional characterization of leishmanial PEPCK. The recombinant Leishmania major enzyme (Lmj_PEPCK) exhibits equivalent kcat values for the phosphoenolpyruvate (PEP) and oxaloacetate (OAA) forming reactions. The apparent Km towards OAA is 10-fold lower than that for PEP, while the Km valu...

journal_title：Archives of biochemistry and biophysics

authors： Sosa MH,Giordana L,Nowicki C

更新日期：2015-10-01 00:00:00

abstract：:In this study, oxygen consumption and H(2)O(2) release rate by succinate or pyruvate/malate supplemented mitochondria isolated from skeletal muscle of trained and untrained rats were investigated. The overall mitochondrial antioxidant capacity and the effect of preincubation of mitochondria with GDP, an inhibitor of u...

journal_title：Archives of biochemistry and biophysics

authors： Venditti P,Masullo P,Di Meo S

更新日期：1999-12-15 00:00:00

abstract：:Increasing evidence suggests that long non-coding RNAs (lncRNAs) are implicated with chemoresistance of cancers. However, their functional role and molecular mechanisms in colorectal cancer (CRC) chemoresistance are still largely unclear. In this work, we aimed to investigate the functional role of lncRNA cancer susce...

journal_title：Archives of biochemistry and biophysics

authors： Gao R,Fang C,Xu J,Tan H,Li P,Ma L

更新日期：2019-03-15 00:00:00

abstract：:An increased permeability of the intestinal barrier is proposed as a major event in the pathophysiology of inflammatory bowel diseases (IBD). Tumor necrosis alpha (TNFα) plays a central role in IBD pathogenesis, in part promoting tight function (TJ) barrier dysfunction. Food extracts enriched in (-)-epicatechin (EC) p...

journal_title：Archives of biochemistry and biophysics

authors： Contreras TC,Ricciardi E,Cremonini E,Oteiza PI

更新日期：2015-05-01 00:00:00