Glutamine transport in normal and acidotic rat kidney mitochondria.

abstract：:A role of cysteine residues in annexin II tetramer (AIIt)'s function was investigated using the sulfhydryl reagent N-ethylmaleimide (NEM). Incubation of AIIt with NEM resulted in a dose-dependent inhibition of AIIt-mediated liposome aggregation and loss of sulfhydryl groups of AIIt. The concentration effecting 50% inh...

journal_title：Archives of biochemistry and biophysics

authors： Singh TK,Liu L

更新日期：2000-09-15 00:00:00

abstract：:Thrombin induces platelet activation through a variety of intracellular mechanisms, including Ca(2+) mobilization. The protein of the exocytotic machinery SNAP-25, but not VAMPs, is required for store-operated Ca(2+) entry, the main mechanism for Ca(2+) influx in platelets. Hence, we have investigated the role of the ...

journal_title：Archives of biochemistry and biophysics

authors： Jardin I,Ben Amor N,Hernández-Cruz JM,Salido GM,Rosado JA

更新日期：2007-09-01 00:00:00

abstract：:Purple Cu(A) centers are a class of binuclear, mixed-valence copper complexes found in cytochrome c oxidase and nitrous oxide reductase. An engineered Cu(A) protein was formed by replacing a portion of the amino acid sequence that contains three of the ligands to the native type I copper center of Pseudomonas aerugino...

journal_title：Archives of biochemistry and biophysics

authors： Sun D,Wang X,Davidson VL

更新日期：2002-08-01 00:00:00

abstract：:The stoichiometry of complex formation between two lysosomal proteinases from rabbit liver, cathepsin M and fructose 1,6-bisphosphatase converting enzyme (CE), and their respective endogenous inhibitors was studied by the equilibrium gel penetration method. In each case the molecular weight of the complex was found to...

journal_title：Archives of biochemistry and biophysics

authors： Pontremoli S,Melloni E,Salamino F,Sparatore B,Michetti M,Horecker BL

更新日期：1984-02-01 00:00:00

abstract：:Two isoforms of regulatory (R) subunit of cAMP-dependent protein kinase (PKA), named R(myt1) and R(myt2), were identified so far in the sea mussel Mytilus galloprovincialis. Out of them, only R(myt2) was phosphorylated in vitro by casein kinase 2 (CK2) using GTP as phosphate donor. CK2 catalytic subunit (CK2alpha) its...

journal_title：Archives of biochemistry and biophysics

authors： Bardales JR,Hellman U,Villamarín JA

更新日期：2007-05-01 00:00:00

abstract：:This paper describes the effect of an ionic (sodium dodecyl sulfate; SDS) and a nonionic detergent (Triton X-100) on the substrate and enzyme components of the lecithin: cholesterol acyltransferase (LCAT) reaction. When the enzyme sources (purified or partially purified) or the respective substrates [high-density lipo...

journal_title：Archives of biochemistry and biophysics

authors： Park MC,McConathy WJ,Lacko AG

更新日期：1984-08-15 00:00:00

abstract：:Ovarian cancer is among the most prevalent and lethal types of cancers in women. Several factors such as late diagnosis, unavailability of the reliable biomarkers, frequent relapses and dearth of efficient therapeutic targets form bottleneck in the treatment of ovarian cancers. In this study we investigated the potent...

journal_title：Archives of biochemistry and biophysics

authors： Zhao R,Liu Q,Lou C

更新日期：2018-08-01 00:00:00

abstract：:Previous studies have shown that reactive oxygen species (ROS) enhance arachidonic acid (AA) release and the subsequent AA metabolism in macrophages. The purpose of this study was determined the implication of phospholipases A2 (PLA2s) in these events. Our results show that oxidative stress induced by exogenous adding...

journal_title：Archives of biochemistry and biophysics

authors： Martínez J,Moreno JJ

更新日期：2001-08-15 00:00:00

abstract：:A rabbit liver UDP-glucuronosyltransferase cDNA that is related to human and rat UGT1A7 has been identified. The predicted amino acid sequence of the UGT1A7l displays 80% similarity to that encoded by human HP4 (UGT1A9), but 81% to that predicted for human UGT1A7 and 77% to the rat UGT1A7 (UGTA2). The exons encoding h...

journal_title：Archives of biochemistry and biophysics

authors： Bruck M,Li Q,Lamb JG,Tukey RH

更新日期：1997-08-15 00:00:00

abstract：:In an effort to model the interaction of lipid-based DNA delivery systems with anionic surfaces, such as a cell membrane, we have utilized microelectrophoresis to characterize how electrokinetic measurements can provide information on surface charge and binding characteristics. We have established that cationic lipids...

journal_title：Archives of biochemistry and biophysics

authors： Wong FM,Bally MB,Brooks DE

更新日期：1999-06-01 00:00:00

abstract：:A cDNA clone encoding troponin C of Akazara scallop (Chlamys nipponensis akazara) striated adductor muscle was isolated and sequenced. The cDNA is composed of 1987 bp and has an open reading frame of 462 bp at nucleotide positions 12-473. The amino acid sequence deduced from the cDNA showed 89% homology with that of E...

journal_title：Archives of biochemistry and biophysics

authors： Ojima T,Tanaka H,Nishita K

更新日期：1994-06-01 00:00:00

abstract：:Periodate-oxidized NADP+ (dialdehyde-NADP+) inactivated soluble ferredoxin-NADP+ oxidoreductase and combined covalently to the enzyme. This inactivation was first order with respect to dialdehyde-NADP+ and followed saturation kinetics, indicating that the enzyme initially forms a reversible complex with the inactivato...

journal_title：Archives of biochemistry and biophysics

authors： Chan RL,Carrillo N

更新日期：1984-02-15 00:00:00

abstract：:The fundamental improvement of muscle ischemia requires the re-establishment of sufficient vessel network. Despite many kinds of drugs have been used for ischemia, effective angiogenic drug is very limited. Here, we reported the identification and isolation of a potent angiogenic fraction (angio-T) from Geum japonicum...

journal_title：Archives of biochemistry and biophysics

authors： Cheung WL,Cheng L,Liu H,Gu X,Li M

更新日期：2007-03-01 00:00:00

abstract：:A number of natural or synthetic compounds as AGE inhibitors have been proposed, discovered or currently being advanced by others and us. We have identified two new classes of aromatic compounds; aryl- (and heterocyclic) ureido and aryl (and heterocyclic) carboxamido phenoxyisobutyric acids, and benzoic acid derivativ...

journal_title：Archives of biochemistry and biophysics

authors： Rahbar S,Figarola JL

更新日期：2003-11-01 00:00:00

abstract：:Dihydrocaffeic acid, a dietary constituent and a microbial metabolite of flavonoids, is an antioxidant, but few biological effects have been examined. After its production by microflora in the colon, dihydrocaffeic acid is absorbed and found in plasma as a combination of free and metabolized forms. Excess solar UV rad...

journal_title：Archives of biochemistry and biophysics

authors： Poquet L,Clifford MN,Williamson G

更新日期：2008-08-15 00:00:00

abstract：:This paper describes a theory for the mechanism of three-state transition of proteins which is often observed in aqueous organic cosolvent systems, i.e., from the native, via intermediate to helical forms. The first transition, accompanied by changes in the tertiary and/or secondary structures, was explained by larger...

journal_title：Archives of biochemistry and biophysics

authors： Arakawa T,Goddette D

更新日期：1985-07-01 00:00:00

abstract：:We propose that adverse effects of the antibiotic metronidazole may be due, wholly or in part, to its conversion to a thiamine analog and consequent vitamin B1 antagonism. Consistent with this hypothesis, the drug is accepted as a substrate for the thiaminase (EC 2.5.1.2) elaborated as an exoenzyme by the human gut fl...

journal_title：Archives of biochemistry and biophysics

authors： Alston TA,Abeles RH

更新日期：1987-09-01 00:00:00

abstract：:Intrinsically disordered proteins represent about one third of eukaryotic proteins. An additional third correspond to proteins containing folded domains as well as large intrinsically disordered regions (IDR). While IDRs may represent functionally autonomous domains, in some instances it has become clear that they pro...

journal_title：Archives of biochemistry and biophysics

authors： Arbesú M,Pons M

更新日期：2019-11-30 00:00:00

abstract：:Recently, we characterized a surface antigen (Z-1) of guinea pig macrophages by monoclonal anti-Z-1 antibody. The Z-1 antigen consists of two different polypeptide chains; alpha (140 kDa) and beta (95 kDa). This antigen is closely correlated with the phagocytic activity of the cells for zymosan and presumably function...

journal_title：Archives of biochemistry and biophysics

authors： Hazeki K,Tamoto K,Tada M,Mori Y

更新日期：1989-05-01 00:00:00

abstract：:Spontaneously beating isolated rat atria were subjected to 1 h of anoxia at 37 degrees C in various cardioplegic solutions. Contraction continued for different times upon initiation of anoxia, depending on the nature of the cardioplegic solution. Two hundred micromolar P1,P5-di(adenosine-5')pentaphosphate (Ap5A) stopp...

journal_title：Archives of biochemistry and biophysics

authors： Savabi F,Bessman SP

更新日期：1986-07-01 00:00:00

abstract：:In 5-aminolevulinic acid (ALA)-based photodynamic therapy (PDT), ALA taken up by tumor cells is metabolized to protoporphyrin IX (PpIX), which sensitizes photodamage leading to apoptotic or necrotic cell death. Since lipophilic PpIX originates in mitochondria, we postulated that photoperoxidation of highly unsaturated...

journal_title：Archives of biochemistry and biophysics

authors： Kriska T,Korytowski W,Girotti AW

更新日期：2005-01-15 00:00:00

abstract：:Advancements in peptide fusion technologies to maximize the protein production has taken a big leap to fulfill the demands of post-genomics era targeting elucidation of structure/function of the proteome and its therapeutic applications, by over-expression in heterologous expression systems. Despite being most preferr...

journal_title：Archives of biochemistry and biophysics

authors： Yadav DK,Yadav N,Yadav S,Haque S,Tuteja N

更新日期：2016-12-15 00:00:00

abstract：:The treatment of male rats with Hg2+ resulted in significant alterations in heme and hemoprotein metabolism in the adrenal gland which, in turn, were reflected in abnormal steroidogenic activities and steroid output. Twenty-four hours after the administration of 30 mumol of HgCl2/kg (sc) the mitochondrial heme and cyt...

journal_title：Archives of biochemistry and biophysics

authors： Veltman JC,Maines MD

更新日期：1986-08-01 00:00:00

abstract：:One- and two-dimensional 1H NMR experiments were used to determine the chemical shifts of resonances arising from 29 residues of RNase A in H2O at 17 pH values ranging from 1.2 to 7.9 Nearly all resonances displaying pH-induced changes in chemical shift greater than 0.1 ppm were monitored. Individual plots of the chem...

journal_title：Archives of biochemistry and biophysics

authors： Baker WR,Kintanar A

更新日期：1996-03-01 00:00:00

abstract：:It was shown previously that the ATP synthase complex of bovine heart mitochondria contains an essential set of thiols or dithiols in its membrane sector (F0), whose modification by various reagents results in uncoupling [Yagi, T., and Hatefi, Y. (1984) Biochemistry 23, 2449-2455]. The sensitivity to modifiers was inc...

journal_title：Archives of biochemistry and biophysics

authors： Yagi T,Hatefi Y

更新日期：1987-04-01 00:00:00

abstract：:Evidence indicates that activation of a beta 1----3N-acetylglucosaminyltransferase is responsible for accumulation of large quantities of lacto-series tumor-associated antigens in human colonic adenocarcinomas. Expression of type 1 and 2 core chain derivatives characterize human colonic adenocarcinomas, whereas normal...

journal_title：Archives of biochemistry and biophysics

authors： Holmes EH

更新日期：1989-05-01 00:00:00

abstract：:Although mutation of mitochondrial DNA (mtDNA) in human tissues has been established to associate with intrinsic aging, the impact of environmental factors on the formation and accumulation of mtDNA mutations and oxidative DNA damage in human tissues is poorly understood. We have investigated the levels of mtDNA with ...

journal_title：Archives of biochemistry and biophysics

authors： Lee HC,Lim ML,Lu CY,Liu VW,Fahn HJ,Zhang C,Nagley P,Wei YH

更新日期：1999-02-15 00:00:00

abstract：:The effect of the skeletal myopathy-causing E117K mutation in human β-tropomyosin on actomyosin structure during the ATPase cycle was studied using fluorescent probes bound to actin subdomain 1 and the myosin head. Multistep changes in flexural rigidity of actin filament and in spatial arrangement of actin subdomain 1...

journal_title：Archives of biochemistry and biophysics

authors： Karpicheva OE,Redwood CS,Borovikov YS

更新日期：2014-05-01 00:00:00

abstract：:Structural aspects of the binding of the linear ligands N alpha-acetyl-L-lysine (AcLys) and epsilon-aminocaproic acid (epsilon ACA) and of the cyclic analogs trans-(aminomethyl)-cyclohexanecarboxylic acid (AMCHA) and p-benzylaminesulfonic acid (BASA) to the intact plasminogen kringle 4 domain have been investigated by...

journal_title：Archives of biochemistry and biophysics

authors： De Marco A,Laursen RA,Llinas M

更新日期：1986-02-01 00:00:00

abstract：:Abnormal production of matrix metalloproteinases (MMPs) has been observed in a variety of diseases, such as emphysema, atherosclerosis, and cancer metastasis. Destruction of connective tissue ensues and elastin is often a key target. Three of the main elastolytic MMPs are the gelatinases MMP-2 and MMP-9 and the metall...

journal_title：Archives of biochemistry and biophysics

authors： Jensen SA,Andersen P,Vrhovski B,Weiss AS

更新日期：2003-01-15 00:00:00