Pectin microgels and their subunit structure.

abstract：:Small compounds that activate the insulin-dependent signaling pathway have potential therapeutic applications in controlling type 2 diabetes mellitus. The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is an insulin-inducible transcription factor that decreases expression of the phosphoenolpyruvate carbo...

journal_title：Archives of biochemistry and biophysics

authors： Haneishi A,Takagi K,Asano K,Yamamoto T,Tanaka T,Nakamura S,Noguchi T,Yamada K

更新日期：2012-09-01 00:00:00

abstract：:The purpose of the current study is to present further evidence for prothrombin self-association as assessed by chemical crosslinking. When the self-association (evaluated by covalent crosslinking with dithiobis(succinimidylpropionate) of prothrombin or fragment 1 was evaluated at the same molar concentration of prote...

journal_title：Archives of biochemistry and biophysics

authors： Tarvers RC,Roberts HR,Straight DL,Featherstone GL,Lundblad RL

更新日期：1987-09-01 00:00:00

abstract：:Uridine phosphorylase is one of the critical enzymes in the pyrimidine salvage pathway. Cells regenerate uridine for nucleotide metabolism by incorporating uracil with ribose-1-phosphate with this enzyme. Recent studies indicate that Escherichia coli uridine phosphorylase is destabilized in the presence of ATP. Howeve...

journal_title：Archives of biochemistry and biophysics

authors： Liu YK,Lin TH,Liu PF

更新日期：2017-11-15 00:00:00

abstract：:1-O-Alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC) induced phosphorylation of two proteins having molecular masses of approximately 20- and 40-kDa in washed rabbit platelets in a concentration- and time-dependent manner. Sequential stimulation with AGEPC did not induce additional protein phosphorylation, supportin...

journal_title：Archives of biochemistry and biophysics

authors： Sugatani J,Hanahan DJ

更新日期：1986-05-01 00:00:00

abstract：:Human sorcin, M(r) 22-kDa/pI 5.3, was identified in HOB1 lymphoma cells resistant to 1.0 microM vincristine and polyclonal antibody against it was produced. Using antibody to precipitate sorcin from 32P-labeled cell extract, the SDS-PAGE-resolved human sorcin was not phosphorylated in contrast to the report of A. M. v...

journal_title：Archives of biochemistry and biophysics

authors： Lee WP

更新日期：1996-01-15 00:00:00

abstract：:Secreted frizzled-related protein (sFRP)-1 is a Wnt antagonist that inhibits breast carcinoma cell motility, whereas the secreted glycoprotein thrombospondin-1 stimulates adhesion and motility of the same cells. We examined whether thrombospondin-1 and sFRP-1 interact directly or indirectly to modulate cell behavior. ...

journal_title：Archives of biochemistry and biophysics

authors： Martin-Manso G,Calzada MJ,Chuman Y,Sipes JM,Xavier CP,Wolf V,Kuznetsova SA,Rubin JS,Roberts DD

更新日期：2011-05-15 00:00:00

abstract：:In order to characterize cellular retinoic acid-binding protein (CRABP) from chick embryos, we resolved the [3H]RA-binding activity of embryo extracts by Mono Q anion-exchange chromatography. Fractions containing [3H]RA-binding proteins eluted in two peaks at 7 and 10 min with the latter showing 10-fold higher activit...

journal_title：Archives of biochemistry and biophysics

authors： Venepally P,Reddy LG,Sani BP

更新日期：1996-12-15 00:00:00

abstract：:FOXP2 is a transcriptional repressor involved in development of the human brain and is the first gene product to be linked to the evolution of human speech. FOXP2 belongs to the FOX superfamily of proteins that share a common winged helix DNA binding domain - the forkhead domain. A divalent cation (Mg2+ or Ca2+) has b...

journal_title：Archives of biochemistry and biophysics

authors： Morris G,Pahad N,Dirr HW,Fanucchi S

更新日期：2018-11-01 00:00:00

abstract：:The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is a basic helix-loop-helix transcription factor. The hepatic expression of SHARP-2 mRNA was investigated under various conditions. The level was decreased in the regenerating rat liver and malignant hepatoma cells. In contrast, the expression of SHARP-2...

journal_title：Archives of biochemistry and biophysics

authors： Hirano S,Yamada K,Kawata H,Shou Z,Mizutani T,Shigematsu Y,Mayumi M,Miyamoto K

更新日期：2004-02-01 00:00:00

abstract：:The excimer fluorescence of pyrene iodoacetamide-labeled tropomyosin bound to actin is a probe of the regulatory state change of the muscle thin filament associated with the cooperative binding of myosin subfragment 1 (S1) (Y. Ishii and S. S. Lehrer, Biochemistry 29, 1160, 1990). In this study, the excimer fluorescenc...

journal_title：Archives of biochemistry and biophysics

authors： Ishii Y,Lehrer SS

更新日期：1993-08-15 00:00:00

abstract：:The oxygenation pattern of the essential oil monoterpenes of commercial mint (Mentha) species is determined by regiospecific cytochrome P450-catalyzed hydroxylation of the common olefinic precursor (-)-4S-limonene. In spearmint (M. spicata), C6-allylic hydroxylation leads to (-)-trans-carveol and thence (-)-carvone, w...

journal_title：Archives of biochemistry and biophysics

authors： Haudenschild C,Schalk M,Karp F,Croteau R

更新日期：2000-07-01 00:00:00

abstract：:Triosephosphate isomerase from rabbit has 5 Cys and 2 Met, while triosephosphate isomerase from yeast has 2 Cys (present in the rabbit enzyme in equivalent positions) and no Met. Since chloramine-T oxidizes Cys and Met, we determined the effect it has on the activity and structure of both enzymes. The activity of trio...

journal_title：Archives of biochemistry and biophysics

authors： Zubillaga RA,Pérez-Montfort R,Gómez-Puyou A

更新日期：1994-09-01 00:00:00

abstract：:Drought is one of the most serious factors affecting crop yields in the world. Macleaya cordata (Willd.) is a draught-tolerant medicinal plant that has been proposed as a pioneer crop to be cultivated in arid areas. However, the exact molecular mechanisms through which M. cordata responds to draught stress remain elus...

journal_title：Archives of biochemistry and biophysics

authors： Yu L,Zhou L,Liu W,Huang P,Jiang R,Tang Z,Cheng P,Zeng J

更新日期：2020-05-15 00:00:00

abstract：:The rates of reactive absorption of ozone by various biomolecule solutions were measured. At pH 7, the ability of various biomolecules to reactively absorb ozone was in the following sequence: thiosulfate > ascorbate > cysteine approximately methionine > glutathione. The rates of reactive absorption under a variety of...

journal_title：Archives of biochemistry and biophysics

authors： Kanofsky JR,Sima PD

更新日期：1995-01-10 00:00:00

abstract：:Complementary peptides to interleukin-2 (IL-2) sequences important for receptor binding were tested for their ability to mimic natural receptors and act as inhibitors of the IL-2/p55 receptor subunit interaction. Peptides hydropathically complementary to IL-2 sequences 15-27 and 40-54 were synthesized in a linear and ...

journal_title：Archives of biochemistry and biophysics

authors： Fassina G,Cassani G,Gnocchi P,Fornasiero MC,Isetta AM

更新日期：1995-04-01 00:00:00

abstract：:Cystathionine beta-lyase, the second enzyme of the transsulfuration pathway leading to homocysteine synthesis was purified over 16,000-fold from spinach (Spinacia oleracea L.) leaf chloroplasts (soluble fraction). Enzyme activity was followed along the purification scheme by either a colorimetric method for the determ...

journal_title：Archives of biochemistry and biophysics

authors： Droux M,Ravanel S,Douce R

更新日期：1995-01-10 00:00:00

abstract：:The occurrence of a variety of purine receptors in the immune system indicates that extracellular purines play important functional roles. Extracellular purine concentrations are, in great part, determined by ectonucleotidases, namely, the ATP diphosphohydrolase, also identified as CD39, a lymphocyte cell surface mark...

journal_title：Archives of biochemistry and biophysics

authors： Benrezzak O,Grondin G,Sévigny J,Gendron FP,Rousseau E,D'Orléans-Juste P,Beaudoin AR

更新日期：1999-10-15 00:00:00

abstract：:We have previously produced 12 monoclonal antibodies (MAbs) against rat cytochrome P450(CYP)2B1, and 8 of these inhibit CYP2B1 catalytic activity to varying extents. Using competitive binding studies we showed that this collection of 12 MAbs recognize at least 6 spatially distinct epitopes. The complete coding sequenc...

journal_title：Archives of biochemistry and biophysics

authors： Parimoo B,Thomas PE

更新日期：2000-08-01 00:00:00

abstract：:Serine is an essential amino acid for the lectin-mediated transformation of human peripheral blood lymphocytes due to the inability of this cell to synthesize sufficient quantities via either the phosphorylated pathway or by reversal of the serine hydroxymethyltransferase reaction to meet the metabolic demands. The le...

journal_title：Archives of biochemistry and biophysics

authors： Rowe PB,Sauer D,Fahey D,Craig G,McCairns E

更新日期：1985-01-01 00:00:00

abstract：:Glycosylasparaginase (EC 3.5.1.26) is a lysosomal amidase which hydrolyzes the bond between asparagine and the sugar moiety in N-linked glycoproteins. Deficiency of the enzyme results in aspartylglycosaminuria (AGU), the most common disorder of glycoprotein degradation. Mature enzyme is formed by two proteolytic cleav...

journal_title：Archives of biochemistry and biophysics

authors： Park H,Vettese-Dadey M,Aronson NN Jr

更新日期：1996-04-01 00:00:00

abstract：:The reactive cellular products generated during the peroxidation of membrane lipids have been implicated as causative agents in a variety of degenerative diseases and aging. In particular, 4-hydroxynon-2-enal (4HNE) is among the most of the produced during lipid peroxidation. In humans and rodent species, the alpha 4 ...

journal_title：Archives of biochemistry and biophysics

authors： Gardner JL,Gallagher EP

更新日期：2001-06-01 00:00:00

abstract：:The various interactions of rat liver dihydrofolate reductase with two unconjugated 7,8-dihydropteridines, 7,8-dihydrobiopterin and 6-methyl-7,8-dihydropteridine, have been compared with those of 7,8-dihydrofolate and folate. Of particular interest was the reactivity demonstrated by 7,8-dihydrobiopterin because of the...

journal_title：Archives of biochemistry and biophysics

authors： Webber S,Whiteley JM

更新日期：1985-02-01 00:00:00

abstract：:Truncation of 112 amino acids at the N-terminus (Nd(1-112)) changes the chain transfer pattern of the Escherichia coli glycogen branching enzyme (GBE) [Arch. Biochem. Biophys. 397 (2002) 279]. We investigated further the role of the N-terminus by engineering other truncated GBEs and analyzing the branching pattern by ...

journal_title：Archives of biochemistry and biophysics

authors： Devillers CH,Piper ME,Ballicora MA,Preiss J

更新日期：2003-10-01 00:00:00

abstract：:In the presence of rat liver cytosol and homocysteine, trimethylselenonium ion (TMSe+) underwent time-dependent demethylation to dimethylselenide with the concurrent formation of methionine. Convenient methods were developed for assay of this activity using either radioactive methods based on the gamma emitting isotop...

journal_title：Archives of biochemistry and biophysics

authors： Goeger DE,Ganther HE

更新日期：1993-04-01 00:00:00

abstract：:We have designed a Hoogsteen (HG) triple-helical three-way junction (ternary complex) constructed from three 33-mer oligonucleotides based on the same subset of sequences used for the Watson-Crick (WC) triple-helical three-way junction, characterized previously (P. L. Hüsler and H. H. Klump (1994) Arch. Biochem. Bioph...

journal_title：Archives of biochemistry and biophysics

authors： Hüsler PL,Klump HH

更新日期：1995-09-10 00:00:00

abstract：:Aryl hydrocarbon hydroxylase activity is most closely associated with cytochrome P-450c in the rat and cytochrome P1-450 in the mouse. The sequence for the orthologous human gene coding for this enzymatic activity has been determined from several sources: cytochrome P-450c isolated from human embryonic DNA [K. Kawajir...

journal_title：Archives of biochemistry and biophysics

authors： Iversen PL,Heiger WJ,Bresnick E,Hines RN

更新日期：1987-07-01 00:00:00

abstract：:The oxidizing power of the thiyl radical (GS*) produced on oxidation of glutathione (GSH) was determined as the mid-point electrode potential (reduction potential) of the one-electron couple E(m)(GS*,H+/GSH) in water, as a function of pH over the physiological range. The method involved measuring the equilibrium const...

journal_title：Archives of biochemistry and biophysics

authors： Madej E,Wardman P

更新日期：2007-06-01 00:00:00

abstract：:Nasal cartilage is widely used in reconstructive surgery for the replacement of soft tissue defects and nasal reconstruction procedures. The ability to shape harvested tissue and the performance in the transplant site are related to the mechanical properties of nasal cartilage. Several studies have documented changes ...

journal_title：Archives of biochemistry and biophysics

authors： Rotter N,Tobias G,Lebl M,Roy AK,Hansen MC,Vacanti CA,Bonassar LJ

更新日期：2002-07-01 00:00:00

abstract：:A cDNA clone encoding troponin C of Akazara scallop (Chlamys nipponensis akazara) striated adductor muscle was isolated and sequenced. The cDNA is composed of 1987 bp and has an open reading frame of 462 bp at nucleotide positions 12-473. The amino acid sequence deduced from the cDNA showed 89% homology with that of E...

journal_title：Archives of biochemistry and biophysics

authors： Ojima T,Tanaka H,Nishita K

更新日期：1994-06-01 00:00:00

abstract：:Sequence alignment of pig mitochondrial NADP-dependent isocitrate dehydrogenase with eukaryotic (human, rat, and yeast) and Escherichia coli isocitrate dehydrogenases reveals that Tyr316 is completely conserved and is equivalent to the E. coli Tyr345, which interacts with the 2'-phosphate of NADP in the crystal struct...

journal_title：Archives of biochemistry and biophysics

authors： Lee P,Colman RF

更新日期：2002-05-01 00:00:00