sFRP-1 binds via its netrin-related motif to the N-module of thrombospondin-1 and blocks thrombospondin-1 stimulation of MDA-MB-231 breast carcinoma cell adhesion and migration.

abstract：:The various interactions of rat liver dihydrofolate reductase with two unconjugated 7,8-dihydropteridines, 7,8-dihydrobiopterin and 6-methyl-7,8-dihydropteridine, have been compared with those of 7,8-dihydrofolate and folate. Of particular interest was the reactivity demonstrated by 7,8-dihydrobiopterin because of the...

journal_title：Archives of biochemistry and biophysics

authors： Webber S,Whiteley JM

更新日期：1985-02-01 00:00:00

abstract：:Electrophoretically homogeneous preparations of catalytic subunit (C) of cAMP-dependent protein kinase isolated according to two different procedures from holoenzyme type I and type II from rabbit and from holoenzyme type II from rat skeletal muscle and from bovine cardiac muscle can be separated on carboxymethyl cell...

journal_title：Archives of biochemistry and biophysics

authors： Kinzel V,Hotz A,König N,Gagelmann M,Pyerin W,Reed J,Kübler D,Hofmann F,Obst C,Gensheimer HP

更新日期：1987-03-01 00:00:00

abstract：:The conformation of a constrained peptide mimicking the putative first intracellular domain (iLP1) of thromboxane A(2) receptor (TP) was determined by (1)H 2D NMR spectroscopy. Through completed assignments of TOCSY, DQF-COSY, and NOESY spectra, a NMR structure of the peptide showed a beta-turn in residues 56-59 and a...

journal_title：Archives of biochemistry and biophysics

authors： Geng L,Wu J,So SP,Huang G,Ruan KH

更新日期：2004-03-15 00:00:00

abstract：:The purpose of this study was to investigate the effects of thyroid state on rates and sites of H(2)O(2) production in rat muscle mitochondria. With Complex I- and Complex II-linked substrates, hypothyroidism decreased and hyperthyroidism increased the rates of O(2) consumption during State 4 and State 3 respiration a...

journal_title：Archives of biochemistry and biophysics

authors： Venditti P,Puca A,Di Meo S

更新日期：2003-03-01 00:00:00

abstract：:The dicarbonyl compounds, phenylgloxyl and 2,3-butanedione were used to demonstrate the presence of an essential arginine residue in the mechanism of the red beet (Beta vulgaris L.) plasma membrane ATPase. Treatment of the red beet ATPase with either of these reagents resulted in an inhibition of ATP hydrolytic activi...

journal_title：Archives of biochemistry and biophysics

authors： Gildensoph LH,Briskin DP

更新日期：1989-05-15 00:00:00

abstract：:In this work we demonstrate that aldose reductase (AR) interacts directly with tubulin and, was subjected to microtubule formation conditions, enzymatic AR activity increased more than sixfold. Since AR interacts mainly with tubulin that has 3-nitro-tyrosine in its carboxy-terminal, we evaluated whether tyrosine and o...

journal_title：Archives of biochemistry and biophysics

authors： Rivelli JF,Ochoa AL,Santander VS,Nigra A,Previtali G,Casale CH

更新日期：2018-09-15 00:00:00

abstract：:Diabetic surgical patients are at risk for peri- and post-operative complications, which can be prevented by maintaining tight glycemic control during anesthesia. Control of blood sugar would decrease unwanted chemical reactions, such as protein glycation, minimizing tissue dysfunction. Methylglyoxal (MG) is a major c...

journal_title：Archives of biochemistry and biophysics

authors： Pattin AE,Ochs S,Theisen CS,Fibuch EE,Seidler NW

更新日期：2010-06-01 00:00:00

abstract：:Some compounds are known to induce solute-nonselective permeability of the inner mitochondrial membrane (IMM) in Ca2+-loaded mitochondria. Existing data suggest that this process, following the opening of a mitochondrial permeability transition pore, is preceded by different solute-selective permeable states of IMM. A...

journal_title：Archives of biochemistry and biophysics

authors： Balakirev MY,Zimmer G

更新日期：1998-08-01 00:00:00

abstract：:Antioxidative flavonoids, ubiquitously included in vegetables, fruits and teas, are expected to prevent degenerative diseases. It is unclear, however, whether flavonoids can enter the cellular nuclei and suppress the oxidative damage of DNA. Here, several flavonoids at the physiological concentration of 10 microM were...

journal_title：Archives of biochemistry and biophysics

authors： Kanazawa K,Uehara M,Yanagitani H,Hashimoto T

更新日期：2006-11-15 00:00:00

abstract：:Maitotoxin (MTX) is a highly potent marine toxin that activates both voltage-sensitive and receptor-operated calcium channels in the plasma membrane. This results in calcium overload that rapidly leads to cell death. We now report that maitotoxin (0.1-1 nM) induces calpain activation in both SH-SY5Y neuroblastoma cell...

journal_title：Archives of biochemistry and biophysics

authors： Wang KK,Nath R,Raser KJ,Hajimohammadreza I

更新日期：1996-07-15 00:00:00

abstract：:Cholesterol is one of the major components of membrane lipids. Adjustment of the membrane cholesterol balance is, therefore, pivotal in the maintenance of cellular homeostasis. Cholesterol biosynthesis and uptake are tightly regulated at the transcriptional level through a negative feedback control. The transcription ...

journal_title：Archives of biochemistry and biophysics

authors： Sato R

更新日期：2010-09-15 00:00:00

abstract：:A radiochemical assay was developed for measuring branched-chain alpha-ketoacid dehydrogenase activity of Triton X-100 extracts of freeze-clamped rat liver. The proportion of active (dephosphorylated) enzyme was determined by measuring enzyme activities before and after activation of the complex with a broad-specifici...

journal_title：Archives of biochemistry and biophysics

authors： Harris RA,Powell SM,Paxton R,Gillim SE,Nagae H

更新日期：1985-12-01 00:00:00

abstract：:Tyrosine kinase was extracted from rat spleen. Some 60% of the activity with angiotensin as the substrate was found in the cytoplasmic supernatant obtained at 100,000g. Two kinases in the supernatant, TKI and -II were purified 170- and 600-fold, respectively, by column chromatography. The molecular weights of TKI and ...

journal_title：Archives of biochemistry and biophysics

authors： Hasuma T,Inoue A,Higashi Y,Morisawa S,Yukioka M

更新日期：1990-07-01 00:00:00

abstract：:Saccharopine dehydrogenase catalyzes the NAD-dependent oxidative deamination of saccharopine to l-lysine and α-ketoglutarate. Lysine 99 is within hydrogen-bond distance to the α-carboxylate of the lysine substrate and D319 is in the vicinity of the carboxamide side chain of NADH. Both are conserved and may be importan...

journal_title：Archives of biochemistry and biophysics

authors： Ekanayake DK,West AH,Cook PF

更新日期：2011-10-01 00:00:00

abstract：:The expression and activity of the phenobarbital (PB)-inducible P-450 isozymes, P-450b and P-450e, and the major 3-methylcholanthrene (MC)-inducible form, P-450c, were studied in primary cultures of adult rat hepatocytes in T1, Leibovitz L-15 (L-15), and a modification of Waymouth 752/1 (Way) media. P-450 isozymes in ...

journal_title：Archives of biochemistry and biophysics

authors： Turner NA,Wilson NM,Jefcoate CR,Pitot HC

更新日期：1988-05-15 00:00:00

abstract：:In order to specify that protein labeling is the result of mono-ADP ribosylation, a careful evaluation of the reaction conditions and products is necessary. To investigate the specificity and target proteins of the arginine-specific mono-ADP-ribosyltransferase (mADP-RT) in rabbit skeletal muscle T-tubules (TT) biotin-...

journal_title：Archives of biochemistry and biophysics

authors： Klebl BM,Göpel SO,Pette D

更新日期：1997-11-15 00:00:00

abstract：:The state of the various, spectroscopically distinguishable copper sites (type 1, type 2, and type 3 copper) of human ceruloplasmin was investigated by electron spin resonance (ESR) spectroscopy. The ESR measurements were performed at 100 K and at X-band during the reaction of the protein with either ascorbate or with...

journal_title：Archives of biochemistry and biophysics

authors： Musci G,Bonaccorsi di Patti MC,Calabrese L

更新日期：1993-10-01 00:00:00

abstract：:Lipoxygenase-induced lipid oxidation contributes to plasma lipoprotein oxidation and may be an underlying pathogenic mechanism of atherogenesis. Since inactivation of the vasorelaxant actions of nitric oxide (.NO) plays a critical role in the impaired function of atherosclerotic vessels and because .NO reacts rapidly ...

journal_title：Archives of biochemistry and biophysics

authors： Rubbo H,Parthasarathy S,Barnes S,Kirk M,Kalyanaraman B,Freeman BA

更新日期：1995-12-01 00:00:00

abstract：:Endometrial carcinoma is a type of gynecological cancer that originates in the endometrial epithelial tissue. Due to its high proliferation and ability to invade muscle tissue, it is one of the most common malignant tumors in the female reproductive system. Fatostatin is a small molecule non-sterol diarylthiazole deri...

journal_title：Archives of biochemistry and biophysics

authors： Yao L,Chen S,Li W

更新日期：2020-05-15 00:00:00

abstract：:Nasal cartilage is widely used in reconstructive surgery for the replacement of soft tissue defects and nasal reconstruction procedures. The ability to shape harvested tissue and the performance in the transplant site are related to the mechanical properties of nasal cartilage. Several studies have documented changes ...

journal_title：Archives of biochemistry and biophysics

authors： Rotter N,Tobias G,Lebl M,Roy AK,Hansen MC,Vacanti CA,Bonassar LJ

更新日期：2002-07-01 00:00:00

abstract：:Horseradish peroxidase A1 thermal stability was studied by steady-state fluorescence, circular dichroism and differential scanning calorimetry at pH values of 4, 7 and 10. Changes in the intrinsic protein probes, tryptophan fluorescence, secondary structure, and heme group environment are not coincident. The T(m) valu...

journal_title：Archives of biochemistry and biophysics

authors： Carvalho AS,Melo EP,Ferreira BS,Neves-Petersen MT,Petersen SB,Aires-Barros MR

更新日期：2003-07-15 00:00:00

abstract：:A recent note from our laboratory reported that L-band (1.2 GHz) electron paramagnetic resonance spectroscopy can be utilized in detecting the formation of Cr(V) intermediates from chromate-treated whole mice. Since Cr(V) is thought to be one of the key species in the mechanism of chromate's toxicity, we carried out a...

journal_title：Archives of biochemistry and biophysics

authors： Liu KJ,Shi X,Jiang JJ,Goda F,Dalal N,Swartz HM

更新日期：1995-10-20 00:00:00

abstract：:The vitamin D receptor (VDR) is a member of the nuclear receptor superfamily and plays a central role in the biological actions of vitamin D. VDR regulates the expression of numerous genes involved in calcium/phosphate homeostasis, cellular proliferation and differentiation, and immune response, largely in a ligand-de...

journal_title：Archives of biochemistry and biophysics

authors： Wang Y,Zhu J,DeLuca HF

更新日期：2012-07-01 00:00:00

abstract：:A10 smooth muscle cells, derived from embryonic rat thoracic aorta, responded to the atrial natriuretic factor (ANF) with increased levels of cyclic GMP. These cells possess high-affinity (apparent Kd = 50 pM) plasma membrane receptors for ANF. Internalization of ANF at 37 degrees C was indicated by the following: app...

journal_title：Archives of biochemistry and biophysics

authors： Napier MA,Arcuri KE,Vandlen RL

更新日期：1986-08-01 00:00:00

abstract：:The protein Mop from Haemophilus influenzae is a member of the molbindin family of proteins. Using isothermal titration calorimetry (ITC), Mop was observed to bind molybdate at two distinct sites with a stoichiometry of 8 mol molybdate per Mop hexamer. Six moles of molybdate bound endothermically at high affinity site...

journal_title：Archives of biochemistry and biophysics

authors： Masters SL,Howlett GJ,Pau RN

更新日期：2005-07-01 00:00:00

abstract：:In the present study, the activity of Topoisomerase IIβ (TopoIIβ) is evaluated during peroxide induced double stranded DNA breaks (DSBs) repair in primary neurons. The results showed that the TopoIIβ levels were enhanced during recovery from peroxide mediated damage (PED) along with Ku70, PARP-1, pol beta, and WRN hel...

journal_title：Archives of biochemistry and biophysics

authors： Mandraju R,Chekuri A,Bhaskar C,Duning K,Kremerskothen J,Kondapi AK

更新日期：2011-12-15 00:00:00

abstract：:Magnesium is involved in a wide range of biochemical reactions that are crucial to cell proliferation, differentiation, angiogenesis, and apoptosis. Changes in magnesium availability have been shown to influence biological responses of immuno-inflammatory cells. Equally plausible seems to be an involvement of magnesiu...

journal_title：Archives of biochemistry and biophysics

authors： Wolf FI,Maier JA,Nasulewicz A,Feillet-Coudray C,Simonacci M,Mazur A,Cittadini A

更新日期：2007-02-01 00:00:00

abstract：:The cytosol fraction of an extract of Xenopus laevis ovaries contains a protein inhibitor that can specifically block the activation of calmodulin-sensitive cyclic nucleotide phosphodiesterase (PDE I) found in that tissue. This inhibitor was purified by DEAE-cellulose chromatography, gel filtration on Sephacryl S-200,...

journal_title：Archives of biochemistry and biophysics

authors： Jedlicki E,Orellana O,Allende CC,Allende JE

更新日期：1985-08-15 00:00:00

abstract：:Flavin-dependent monooxygenases catalyze a wide variety of redox reactions in important biological processes and are responsible for the synthesis of highly complex natural products. Although much has been learned about FMO chemistry in the last ~80 years of research, several aspects of the reactions catalyzed by thes...

journal_title：Archives of biochemistry and biophysics

authors： Reis RAG,Li H,Johnson M,Sobrado P

更新日期：2021-01-16 00:00:00

abstract：:Cytochrome P450 reductase was purified to homogeneity from cell suspension cultures of the opium poppy Papaver somniferum, the enzyme was characterized (K(m) cytochrome c, 8.3 microM; K(m) NADPH, 4.2 microM; pH optimum, 8.0; M(r), 80 kDa), and the amino acid sequence of internal peptides was determined. Partial cDNA c...

journal_title：Archives of biochemistry and biophysics

authors： Rosco A,Pauli HH,Priesner W,Kutchan TM

更新日期：1997-12-15 00:00:00