Cloning and sequence of a cDNA encoding Akazara scallop troponin C.

abstract：:In this study, the binding points of MethB and two structurally-related cationic phenoxazine dyes [meldola blue (MB) and nile blue (NB)] to human butyrylcholinesterase (BChE) were investigated by molecular docking and site directed mutagenesis. The comparative inhibitory effects of MethB, MB and NB on recombinant wild...

journal_title：Archives of biochemistry and biophysics

authors： Sezgin Z,Biberoglu K,Chupakhin V,Makhaeva GF,Tacal O

更新日期：2013-04-01 00:00:00

abstract：:The conformation of a constrained peptide mimicking the putative first intracellular domain (iLP1) of thromboxane A(2) receptor (TP) was determined by (1)H 2D NMR spectroscopy. Through completed assignments of TOCSY, DQF-COSY, and NOESY spectra, a NMR structure of the peptide showed a beta-turn in residues 56-59 and a...

journal_title：Archives of biochemistry and biophysics

authors： Geng L,Wu J,So SP,Huang G,Ruan KH

更新日期：2004-03-15 00:00:00

abstract：:Pseudonaja textilis, an Australian Elapid, is known to produce a highly toxic venom. Both protein profiling and N-terminal sequence analysis showed the presence of four new phospholipases A(2) in this venom. Besides being non-lethal, the phospholipase A(2) proteins were found to be moderately active enzymes and they s...

journal_title：Archives of biochemistry and biophysics

authors： Armugam A,Gong N,Li X,Siew PY,Chai SC,Nair R,Jeyaseelan K

更新日期：2004-01-01 00:00:00

abstract：:Vascular smooth muscle cell (VSMC) proliferation is a key event in the progression of atherosclerosis. Induction of both c-fos (through the transcription factor Elk-1) and c-jun, both immediate early genes, is important for the stimulation of VSMC proliferation and migration. It was earlier found that p38 mitogen-acti...

journal_title：Archives of biochemistry and biophysics

authors： Zhao M,Liu Y,Bao M,Kato Y,Han J,Eaton JW

更新日期：2002-04-15 00:00:00

abstract：:Catalytic activity of ISWI chromatin remodelers, which regulate nucleosome positioning on the DNA, depends on interactions of the putative acidic patch in ISWI helicase domain with the N-termini of nucleosomal H4--such, that removal of H4 termini abolishes ISWI remodeling. Acetylation of H4 termini is also known to di...

journal_title：Archives of biochemistry and biophysics

authors： Krajewski WA

更新日期：2016-02-01 00:00:00

abstract：:Recognition of defined carbohydrate structures by boar sperm was studied on the basis of oligosaccharide structures of porcine zona pellucida glycoproteins so far elucidated. Boar sperm abundantly adhered to fetuin-Sepharose beads, moderately to asialofetuin-Sepharose beads, but not at all to galactosidase (beta1-4-li...

journal_title：Archives of biochemistry and biophysics

authors： Mori E,Yoshitani N,Mori T,Takasaki S

更新日期：2000-02-01 00:00:00

abstract：:Uncoupling protein 2 (UCP2) was reported to be involved in insulin-glucose homeostasis, based on well established event that inhibition of UCP2 stimulates insulin secretion in pancreatic beta-cells. However, the role of UCP2 on insulin-stimulated glucose uptake in adipose tissue, which is an indispensable process in i...

journal_title：Archives of biochemistry and biophysics

authors： Zhou H,Zhao J,Zhang X

更新日期：2009-06-01 00:00:00

abstract：:Since experiments with freshly isolated rat hepatocytes have shown that cellular vitamin E is consumed in response to insult by compounds that induce an oxidative stress only after cellular glutathione (GSH) concentrations have been substantially depleted, experiments were performed to determine whether this sequence ...

journal_title：Archives of biochemistry and biophysics

authors： Warren DL,Reed DJ

更新日期：1991-02-15 00:00:00

abstract：:The macrophage mannose receptor is highly susceptible to modulation by a variety of inflammatory and anti-inflammatory agents. Previous studies have demonstrated that mannose receptor activity is dramatically enhanced in rat bone marrow macrophages following treatment with dexamethasone. In the present study we have i...

journal_title：Archives of biochemistry and biophysics

authors： Cowan HB,Vick S,Conary JT,Shepherd VL

更新日期：1992-07-01 00:00:00

abstract：:Laser Raman spectroscopy has been applied to native and dithiothreitol-treated bovine cortical gamma II crystallin to examine the state of the thiol groups and the presence of a putative disulfide bridge. The data reveal significant differences in two key spectral regions. In the thiol stretching region (2500-2600 cm-...

journal_title：Archives of biochemistry and biophysics

authors： Pande J,McDermott MJ,Callender RH,Spector A

更新日期：1989-02-15 00:00:00

abstract：:Cellobiose dehydrogenase (CDH), an extracellular hemoflavoenzyme produced by the cellulose-degrading cultures of Phanerochaete chrysosporium, oxidizes cellobiose to cellobionolactone. CDH has been purified to homogeneity by a five-step purification procedure. The homogeneous CDH is monomeric and has a relative molecul...

journal_title：Archives of biochemistry and biophysics

authors： Bao W,Usha SN,Renganathan V

更新日期：1993-02-01 00:00:00

abstract：:A uridinediphosphateglucose:thiohydroximate glucosyltransferase (EC 2.4.1.-) has been purified 3700-fold from Brassica napus L. seedlings. The enzyme catalyzes the formation of desulfoglucosinolates by transfer of glucose from UDP-glucose to thiohydroximates and is believed to be the second to last step involved in gl...

journal_title：Archives of biochemistry and biophysics

authors： Reed DW,Davin L,Jain JC,Deluca V,Nelson L,Underhill EW

更新日期：1993-09-01 00:00:00

abstract：BACKGROUND:This study was designed to identify novel links between lipid species and disease progression in non-alcoholic fatty liver disease (NAFLD). METHODS:We analyzed lipid species in the liver and plasma of db/db mice fed a choline-deficient l-amino acid-defined, high-fat diet (CDAHFD) using liquid chromatography...

journal_title：Archives of biochemistry and biophysics

authors： Horas H Nababan S,Nishiumi S,Kawano Y,Kobayashi T,Yoshida M,Azuma T

更新日期：2017-06-01 00:00:00

abstract：:Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase [ATP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.49] is completely inactivated by the 2',3'-dialdehyde derivative of ATP (oATP) in the presence of Mn2+. The dependence of the pseudo-first-order rate constant on reagent concentration indicates t...

journal_title：Archives of biochemistry and biophysics

authors： Saavedra C,Araneda S,Cardemil E

更新日期：1988-11-15 00:00:00

abstract：:Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (SceADH) binds NAD+ and NADH less tightly and turns over substrates more rapidly than does horse (Equus caballus) liver alcohol dehydrogenase E isoenzyme (EcaADH), and neither enzyme uses NADP efficiently. Amino acid residues in the proposed adenylate binding po...

journal_title：Archives of biochemistry and biophysics

authors： Fan F,Plapp BV

更新日期：1999-07-15 00:00:00

abstract：:The keto form of oxaloacetate (OAA), a product of phosphoenolpyruvate carboxylase (PEPC) activity, can undergo various nonenzymatic conversions which make conventional methods of assaying the enzyme difficult, because the products may either act as inhibitors or go undetected. In studies with PEPC isolated from leaves...

journal_title：Archives of biochemistry and biophysics

authors： Walker GH,Ku MS,Edwards GE

更新日期：1986-08-01 00:00:00

abstract：:Nitroxides stable radicals are unreactive toward most diamagnetic molecules, but readily undergo one-electron redox reactions with paramagnetic species such as free radicals and transition metals, thus serving as cell-permeable antioxidants. The cytotoxicity of juglone (5-hydroxy-1,4-naphthoquinone), like that of othe...

journal_title：Archives of biochemistry and biophysics

authors： Zhang R,Hirsch O,Mohsen M,Samuni A

更新日期：1994-08-01 00:00:00

abstract：:The nucleotide sequence of tyrosine tRNAQ* psi A from bovine liver was determined to be pC-C-U-U-C-m2G-A-U-A-m2G-C-U-C-A-G-D-D-G-G-acp3U-A-G-A -G-C-m22G-m22G -A-G-G-A-C-U-Q*-psi-A-m1G-A-psi m-C-C-U-U-A-G-m7G-D-m5C-G-C-U-G-G-T-psi-C-G-m1A -U-U-C-C-G-G-C-U-C-G-A-A-G-G-A-C-C-AOH. This tyrosine tRNA is 76 nucleotides in l...

journal_title：Archives of biochemistry and biophysics

authors： Johnson GD,Pirtle IL,Pirtle RM

更新日期：1985-01-01 00:00:00

abstract：:An anionic peroxidase (EC 1.11.1.7), thought to be involved in suberization, was purified 110-fold from wound-healing slices of Solanum tuberosum by a combination of ammonium sulfate fractionation, Sephadex G-100 gel filtration, isoelectric focusing, and phenyl-Sepharose CL-4B chromatography in 24% yield. The purified...

journal_title：Archives of biochemistry and biophysics

authors： Espelie KE,Kolattukudy PE

更新日期：1985-08-01 00:00:00

abstract：:A number of naturally occurring small organic molecules, primarily involved in maintaining osmotic pressure in the cell, display chaperone-like activity, stabilizing the native conformation of proteins, and protecting them from various kinds of stress. Most of them are sugars, polyols, amino acids or methylamines. Sim...

journal_title：Archives of biochemistry and biophysics

authors： Zancan P,Sola-Penna M

更新日期：2005-12-01 00:00:00

abstract：:The cellulose synthase protein (AcsAB) is encoded by a single gene in Gluconacetobacter hansenii ATCC 23769. We have examined the processing pattern of this enzyme and the localization of the cleavage products by heterologously expressing the truncated portions of the AcsAB protein and using specific antibodies genera...

journal_title：Archives of biochemistry and biophysics

authors： Iyer PR,Liu YA,Deng Y,McManus JB,Kao TH,Tien M

更新日期：2013-01-15 00:00:00

abstract：:Previously, we found that mevalonate-derived products together with an oxysterol regulated reductase synthesis at a posttranscriptional level. To determine which products were responsible for this regulation, either the squalene synthase inhibitor zaragozic acid A or the squalene cyclase inhibitor 4,4,10-beta-trimethy...

journal_title：Archives of biochemistry and biophysics

authors： Peffley DM,Gayen AK

更新日期：1997-01-15 00:00:00

abstract：:Diquat is a hepatotoxin whose toxicity in vivo and in vitro is mediated by redox cycling and greatly enhanced by pretreatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), an inhibitor of glutathione reductase. The mechanism by which redox cycling mediates diquat cytotoxicity is unclear, however. Here, we have att...

journal_title：Archives of biochemistry and biophysics

authors： Sandy MS,Moldeus P,Ross D,Smith MT

更新日期：1987-11-15 00:00:00

abstract：:High-performance size exclusion chromatography revealed that alkaline-extracted peach fruit pectin dissolved in 0.05 M NaNO3 comprised a hierarchy of at least four aggregated, macromolecular-sized species. Each of the three largest species was found to be comparable in length to the three smallest subunits of an inter...

journal_title：Archives of biochemistry and biophysics

authors： Fishman ML,Cooke P,Levaj B,Gillespie DT,Sondey SM,Scorza R

更新日期：1992-04-01 00:00:00

abstract：:The lysosomal cysteine proteinases, cathepsins B, H, and L, were all shown to bind to alpha 2-macroglobulin. The bound enzymes remained active against low-molecular-weight synthetic substrates and bound the active-site-directed inhibitor, benzyloxycarbonyl-125I-Tyr-Ala-diazomethane. Binding of the radiolabeled inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Mason RW

更新日期：1989-09-01 00:00:00

abstract：:Glutathione S-transferases (GSTs) of Plasmodium parasites are potential targets for antimalarial drug and vaccine development. We investigated the equilibrium unfolding, functional activity regulation and stability characteristics of the unique GST of Plasmodium vivax (PvGST). Despite high sequence, structural, functi...

journal_title：Archives of biochemistry and biophysics

authors： Tripathi T,Na BK,Sohn WM,Becker K,Bhakuni V

更新日期：2009-07-15 00:00:00

abstract：:We have previously identified and characterized GSHPx-GI, which is a cellular selenium-dependent glutathione peroxidase (GSHPx) distinct from the classic GSHPx-1 and phospholipid hydroperoxide glutathione peroxidase (PHGPX). We have determined the level of GSHPx-GI mRNA expression in the rat gastrointestinal tract fro...

journal_title：Archives of biochemistry and biophysics

authors： Chu FF,Esworthy RS

更新日期：1995-11-10 00:00:00

abstract：:The Metabotropic glutamate receptor 2 (mGluR2) is involved in several neurological and psychiatric disorders and is an attractive drug target. It is believed to form a strict dimer and the dimeric assembly is necessary for glutamate induced activation. Although many studies have focused on glutamate induced conformati...

journal_title：Archives of biochemistry and biophysics

authors： Singh DR,Pandey K,Mishra AK,Pandey P,Vivcharuk V

更新日期：2021-01-15 00:00:00

abstract：:The formation of alpha-hydroxyethyl radical from ethanol by deermouse microsomes supplemented with NADPH has been demonstrated with the EPR technique of spin trapping with alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (POBN) as the spin trap, in the presence of deferoxamine mesylate. Superoxide dismutase prevented the fo...

journal_title：Archives of biochemistry and biophysics

authors： Knecht KT,Thurman RG,Mason RP

更新日期：1993-06-01 00:00:00

abstract：:The adrenal gland synthesizes steroid hormones from the adrenal cortex and catecholamines from the adrenal medulla. Both cortisol and adrenal androgens can have powerful effects on bone. The overproduction of cortisol in Cushing's disease leads to a dramatic reduction in bone density and an increase risk of fracture. ...

journal_title：Archives of biochemistry and biophysics

authors： Hardy R,Cooper MS

更新日期：2010-11-01 00:00:00