One-carbon metabolism in lectin-activated human lymphocytes.


:Serine is an essential amino acid for the lectin-mediated transformation of human peripheral blood lymphocytes due to the inability of this cell to synthesize sufficient quantities via either the phosphorylated pathway or by reversal of the serine hydroxymethyltransferase reaction to meet the metabolic demands. The level of intracellular serine is tightly regulated, and the culture medium concentration for optimum cellular transformation falls within a relatively narrow range. The three-carbon atom of serine is the major source of one-carbon units required for purine and pyrimidine nucleotide biosynthesis, but the key effect of both serine deprivation and of high medium serine levels would appear to be on protein synthesis. Although an alternative source of one-carbon units, as provided by high levels of formate in the culture medium, can partially reverse the effects of serine deprivation, the only other demonstrable source of one-carbon units, tryptophan, requires serine for its incorporation and subsequent metabolism. Methionine is also essential for lymphocyte transformation and is involved in the synthesis of a small amount of phosphatidylcholine, although most of this phospholipid is provided by choline and lysophosphatidylcholine from the serum-supplemented culture medium.


Arch Biochem Biophys


Rowe PB,Sauer D,Fahey D,Craig G,McCairns E




Has Abstract


1985-01-01 00:00:00














  • Inhibition of protein tyrosine phosphatases PTP1B and CD45 by sulfotyrosyl peptides.

    abstract::Sulfotyrosyl peptides corresponding to the known high-affinity substrate phosphotyrosyl peptide sequences in casein and the autophosphorylation sites of insulin receptor and EGF receptor were investigated as inhibitors of protein tyrosine phosphatases PTP1B and CD45. These peptides inhibit both PTP1B and CD45 in the m...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Desmarais S,Jia Z,Ramachandran C

    更新日期:1998-06-15 00:00:00

  • Role of superoxide and trace transition metals in the production of alpha-hydroxyethyl radical from ethanol by microsomes from alcohol dehydrogenase-deficient deermice.

    abstract::The formation of alpha-hydroxyethyl radical from ethanol by deermouse microsomes supplemented with NADPH has been demonstrated with the EPR technique of spin trapping with alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (POBN) as the spin trap, in the presence of deferoxamine mesylate. Superoxide dismutase prevented the fo...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Knecht KT,Thurman RG,Mason RP

    更新日期:1993-06-01 00:00:00

  • Cytotoxicity of the redox cycling compound diquat in isolated hepatocytes: involvement of hydrogen peroxide and transition metals.

    abstract::Diquat is a hepatotoxin whose toxicity in vivo and in vitro is mediated by redox cycling and greatly enhanced by pretreatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), an inhibitor of glutathione reductase. The mechanism by which redox cycling mediates diquat cytotoxicity is unclear, however. Here, we have att...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sandy MS,Moldeus P,Ross D,Smith MT

    更新日期:1987-11-15 00:00:00

  • Deantigenation of human erythrocytes by bacterial glycosidases--evidence for the noninvolvement of medium-sized glycosphingolipids in the Dolichos biflorus lectin hemagglutination.

    abstract::Fresh human A1 erythrocytes, washed and pretreated in phosphate buffer with or without papain, were incubated at 37 degrees C with blood group-degrading enzymes from the human fecal Ruminococcus torques strain IX-70. The effects were assayed as changes in hemagglutination patterns, and blood group activities of alkali...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Falk P,Hoskins LC,Lindstedt R,Svanborg C,Larson G

    更新日期:1991-11-01 00:00:00

  • Macrophage recognition of externalized phosphatidylserine and phagocytosis of apoptotic Jurkat cells--existence of a threshold.

    abstract::Phosphatidylserine (PS) is predominantly confined to the inner leaflet of plasma membrane in cells, but it is externalized on the cell surface during apoptosis. This externalized PS is required for effective phagocytosis of apoptotic cells by macrophages. Because PS trans-bilayer asymmetry is not absolute in different...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Borisenko GG,Matsura T,Liu SX,Tyurin VA,Jianfei J,Serinkan FB,Kagan VE

    更新日期:2003-05-01 00:00:00

  • Redox properties of an engineered purple Cu(A) azurin.

    abstract::Purple Cu(A) centers are a class of binuclear, mixed-valence copper complexes found in cytochrome c oxidase and nitrous oxide reductase. An engineered Cu(A) protein was formed by replacing a portion of the amino acid sequence that contains three of the ligands to the native type I copper center of Pseudomonas aerugino...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sun D,Wang X,Davidson VL

    更新日期:2002-08-01 00:00:00

  • Heparin causes the accumulation of heparan sulfate in cultures of arterial smooth muscle cells.

    abstract::Heparin infusion in an experimental animal model of arterial injury causes a significant increase in the proteoglycan (PG) component of the extracellular matrix in the injured arteries (Snow, A. D., Bolender, R. P., Wight, T. N., and Clowes, A. W. (1990) Am. J. Pathol. 137, 313-330). The mechanisms responsible for thi...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Potter-Perigo S,Wight TN

    更新日期:1996-12-01 00:00:00

  • Reactive oxygen species produced by liver mitochondria of rats in sepsis.

    abstract::Reactive oxygen species (ROS) can be generated in experimental shock states through several different mechanisms. We measured ROS production in metabolically active liver mitochondria from rats rendered septic by cecal ligation and puncture. By polarography, the State 4 and State 3 respiration rates of liver mitochond...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Taylor DE,Ghio AJ,Piantadosi CA

    更新日期:1995-01-10 00:00:00

  • Determination of dideoxyosone precursors of AGEs in human lens proteins.

    abstract::Dideoxyosones (DDOs) are intermediates in the synthesis of advanced glycation endproducts (AGEs), such as pentosidine and glucosepane. Although the formation of pentosidine and glucosepane in the human lens has been firmly established, the formation of DDOs has not been demonstrated. The aim of this study was to devel...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Linetsky M,Kaid Johar SR,Meltretter J,Padmanabha S,Parmar T,Vasavada AR,Pischetsrieder M,Nagaraj RH

    更新日期:2011-10-01 00:00:00

  • Effects of air pollution particles (ultrafine and fine particulate matter) on mitochondrial function and oxidative stress - Implications for cardiovascular and neurodegenerative diseases.

    abstract::Environmental pollution is a major cause of global mortality and burden of disease. All chemical pollution forms together may be responsible for up to 12 million annual excess deaths as estimated by the Lancet Commission on pollution and health as well as the World Health Organization. Ambient air pollution by particu...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Daiber A,Kuntic M,Hahad O,Delogu LG,Rohrbach S,Di Lisa F,Schulz R,Münzel T

    更新日期:2020-12-15 00:00:00

  • 1,4-Benzoquinone reductase from basidiomycete Phanerochaete chrysosporium: spectral and kinetic analysis.

    abstract::The reaction mechanism of a 1,4-benzoquinone reductase from the wood-rotting basidiomycete Phanerochaete chrysosporium was investigated. The native, oxidized, FMN-containing enzyme was reduced quantitatively by NADH and the resulting reduced enzyme was reoxidized in the presence of one equivalent of 2,6-di-methoxy-1,4...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Brock BJ,Gold MH

    更新日期:1996-07-01 00:00:00

  • Expression and processing of a bacterial endoglucanase in transgenic mice.

    abstract::The C6.5 endoglucanase from Bacillus subtilis catalyzes the hydrolyses of beta-glucans. This enzyme, which is also produced by many ruminant microbes, is not part of the normal digestive repertoire of monogastric animals. We have generated transgenic mice which express the C6.5 endoglucanase gene specifically in the p...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zhang JX,Meidinger R,Forsberg CW,Krell PJ,Phillips JP

    更新日期:1999-07-15 00:00:00

  • MicroRNA-342 targets Cofilin 1 to suppress the growth, migration and invasion of human breast cancer cells.

    abstract::MicroRNA-342-3p (miR-342) has been shown to act as a tumor-suppressor in different cancer types. However, the role and therapeutic implications of miR-342 via modulation of Cofilin 1 (CFL1) has not been studied in any type of cancer. Given the importance of Cofilin signalling in breast, this study was undertaken to ex...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liu C,Xing H,Luo X,Wang Y

    更新日期:2020-07-15 00:00:00

  • Purification and characterization of an inducible aromatic amino acid-sensitive form of chorismate mutase from Solanum tuberosum L. tubers.

    abstract::An amino acid-sensitive form of chorismate mutase (CM) has been purified over 1000-fold from disks excised from tubers of Solanum tuberosum L. cv White Rose. Purification was accomplished by chromatography on Matrix Blue A followed by affinity chromatography with tryptophan as ligand. CM assays performed in the absenc...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kuroki GW,Conn EE

    更新日期:1988-02-01 00:00:00

  • The mouse plasma membrane Ca2+ pump isoform 1 promoter: cloning and characterization.

    abstract::The expression of plasma membrane Ca2+ pump (PMCA) is regulated by various hormones or agonists via multiple second messenger pathways. Two different 5' segments of the PMCA1 gene (isoform 1) were cloned from a mouse genomic library. While one segment contained the 3' end of intron 1 and exon 2, the other segment was ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Du Y,Carlock L,Kuo TH

    更新日期:1995-01-10 00:00:00

  • Rat liver glucose-6-phosphatase system: light scattering and chemical characterization.

    abstract::Glucose-6-phosphatase is a multicomponent system located in the endoplasmic reticulum, involving both a catalytic subunit (G6PC) and several substrate and product carriers. The glucose-6-phosphate carrier is called G6PT1. Using light scattering, we determined K(D) values for phosphate and glucose transport in rat live...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Clottes E,Middleditch C,Burchell A

    更新日期:2002-12-01 00:00:00

  • Regulation of subcellular localization of the aryl hydrocarbon receptor (AhR).

    abstract::The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates the toxicity of dioxin and other xenobiotics. In the absence of exogenous ligand, AhR is cytosolic. We investigated how AhR is retained in the cytosol and how dioxin induces AhR to move to the nucleus. Disruption of nuclear ex...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Richter CA,Tillitt DE,Hannink M

    更新日期:2001-05-15 00:00:00

  • 1,25-Dihydroxyvitamin D₃ suppresses inflammation-induced expression of plasminogen activator inhibitor-1 by blocking nuclear factor-κB activation.

    abstract::Plasminogen activator inhibitor (PAI)-1 is a major fibrinolytic inhibitor. High PAI-1 is associated with increased renal and cardiovascular disease risk. Previous studies demonstrated PAI-1 down-regulation by 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃), but the molecular mechanism remains unknown. Here we show that exposur...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chen Y,Kong J,Sun T,Li G,Szeto FL,Liu W,Deb DK,Wang Y,Zhao Q,Thadhani R,Li YC

    更新日期:2011-03-15 00:00:00

  • Homocysteine-dependent demethylation of trimethylselenonium ion and selenobetaine with methionine formation.

    abstract::In the presence of rat liver cytosol and homocysteine, trimethylselenonium ion (TMSe+) underwent time-dependent demethylation to dimethylselenide with the concurrent formation of methionine. Convenient methods were developed for assay of this activity using either radioactive methods based on the gamma emitting isotop...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Goeger DE,Ganther HE

    更新日期:1993-04-01 00:00:00

  • Site-specific mechanisms of initiation by chelated iron and inhibition by alpha-tocopherol of lipid peroxide-dependent lipid peroxidation in charged micelles.

    abstract::To obtain information on the role of iron-catalyzed lipid peroxidation in the presence of the small amount of lipid peroxide in deterioration of biological membranes, we examined factors affecting peroxidation of fatty acids in charged micelles. Peroxidation of linoleic acid (LA) was catalyzed by Fe2+ via reductive cl...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Fujii T,Hiramoto Y,Terao J,Fukuzawa K

    更新日期:1991-01-01 00:00:00

  • Selectivity in ISG15 and ubiquitin recognition by the SARS coronavirus papain-like protease.

    abstract::The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV PLpro) carries out N-terminal processing of the viral replicase polyprotein, and also exhibits Lys48-linked polyubiquitin chain debranching and ISG15 precursor processing activities in vitro. Here, we used SDS-PAGE and fluorescence-based ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lindner HA,Lytvyn V,Qi H,Lachance P,Ziomek E,Ménard R

    更新日期:2007-10-01 00:00:00

  • Characterization in relation to development of an ecdysteroid agonist-responsive cytochrome P450, CYP18A1, in Lepidoptera.

    abstract::Cytochrome P450 enzymes are involved in a number of steps in ecdysteroid (moulting hormone) homeostasis in insects. We report the cloning and characterization of an ecdysteroid agonist-responsive cytochrome P450, CYP18A1, from the cotton leafworm, Spodoptera littoralis. Northern blot analysis showed that the mRNA tran...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Davies L,Williams DR,Turner PC,Rees HH

    更新日期:2006-09-01 00:00:00

  • Characterization of purified avian 90,000-Da heat shock protein.

    abstract::The so-called 90,000-Da heat shock protein (hsp90) from chicken liver has been purified and physically characterized in the presence of high levels of the serine phosphatase inhibitor fluoride. The protein is an elongated dimer with a molecular weight of 160,000 and a frictional ratio of 1.6. On two-dimensional electr...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Iannotti AM,Rabideau DA,Dougherty JJ

    更新日期:1988-07-01 00:00:00

  • Gossypol inhibition of adenylate cyclase.

    abstract::Gossypol, a polyphenolic binaphthalene -dialdehyde reputed to exert contraceptive action in males, reversibly inhibits adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC] in a concentration-dependent manner. In membranes prepared from a variety of organs, the half-maximal inhibitory concentration (IC50...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Olgiati KL,Toscano DG,Atkins WM,Toscano WA Jr

    更新日期:1984-06-01 00:00:00

  • The application of proteomics for studying the neurorescue activity of the polyphenol (-)-epigallocatechin-3-gallate.

    abstract::Accumulating evidence suggests that oxidative stress resulting in reactive oxygen species generation plays a pivotal role in neurodegenerative diseases, supporting the realization of the use of radical scavengers, metal chelator agents, such as the natural polyphenols for therapy. In this study, we have focused on spe...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Weinreb O,Amit T,Youdim MB

    更新日期:2008-08-15 00:00:00

  • Effect of quercetin on cell protection via erythropoietin and cell injury of HepG2 cells.

    abstract::Quercetin is a flavonoid that has roles in both cytoprotection and cytotoxicity. The relation of queretin's cytoprotective and cytotoxic effects are unknown. Quercetin has been shown to induce expression of hypoxia-inducible factor, a protein that is known to regulate transcription of the erythropoietin (EPO) gene, an...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Nishimura K,Matsumoto R,Yonezawa Y,Nakagawa H

    更新日期:2017-12-15 00:00:00

  • Reconstitution of the lipid-depleted pyruvate oxidase system of Escherichia coli: the palmitic acid effect.

    abstract::Previous work has shown that the coupling of the soluble Escherichia coli pyruvate oxidase to a lipid-depleted membrane terminal electron transport system requires the addition of ubiquinone and a neutral lipid fraction (C. Cunningham and L. P. Hager (1975) J. Biol. Chem. 250, 7139-7146). The active factor present in ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kiuchi K,Hager LP

    更新日期:1984-09-01 00:00:00

  • Induction of cytochrome P-450 1A1 by omeprazole in human HepG2 cells is protein tyrosine kinase-dependent and is not inhibited by alpha-naphthoflavone.

    abstract::Benzimidazole compounds, such as omeprazole and thiabendazole, are a different type of CYP1A1 inducer from Ah receptor-ligands, such as TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and 3-methylcholanthrene. In HepG2 cells, the commonly used tyrosine kinase inhibitors, herbimycin-A and a series of tyrphostins, inhibited ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kikuchi H,Hossain A,Yoshida H,Kobayashi S

    更新日期:1998-10-15 00:00:00

  • Inhibition of peroxidase-catalyzed reactions by deferoxamine.

    abstract::Phagocytes generate superoxide (O2-.) and hydrogen peroxide (H2O2) and their interaction in an iron-catalyzed reaction to form hydroxyl radicals (OH.) (Haber-Weiss reaction) has been proposed. Deferoxamine chelates iron in a catalytically inactive form, and thus inhibition by deferoxamine has been employed as evidence...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Klebanoff SJ,Waltersdorph AM

    更新日期:1988-08-01 00:00:00

  • RPE65, the major retinal pigment epithelium microsomal membrane protein, associates with phospholipid liposomes.

    abstract::The retinal pigment epithelium (RPE)-specific protein RPE65 is the major protein of the RPE microsomal membrane fraction. Though RPE65 lacks transmembrane domains or signal peptide, detergents are required for its maximally effective solubilization in isotonic buffers. However, in 0.75-1.0 M KCl, RPE65 is as soluble w...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Tsilou E,Hamel CP,Yu S,Redmond TM

    更新日期:1997-10-01 00:00:00