One-carbon metabolism in lectin-activated human lymphocytes.

Abstract:

:Serine is an essential amino acid for the lectin-mediated transformation of human peripheral blood lymphocytes due to the inability of this cell to synthesize sufficient quantities via either the phosphorylated pathway or by reversal of the serine hydroxymethyltransferase reaction to meet the metabolic demands. The level of intracellular serine is tightly regulated, and the culture medium concentration for optimum cellular transformation falls within a relatively narrow range. The three-carbon atom of serine is the major source of one-carbon units required for purine and pyrimidine nucleotide biosynthesis, but the key effect of both serine deprivation and of high medium serine levels would appear to be on protein synthesis. Although an alternative source of one-carbon units, as provided by high levels of formate in the culture medium, can partially reverse the effects of serine deprivation, the only other demonstrable source of one-carbon units, tryptophan, requires serine for its incorporation and subsequent metabolism. Methionine is also essential for lymphocyte transformation and is involved in the synthesis of a small amount of phosphatidylcholine, although most of this phospholipid is provided by choline and lysophosphatidylcholine from the serum-supplemented culture medium.

journal_name

Arch Biochem Biophys

authors

Rowe PB,Sauer D,Fahey D,Craig G,McCairns E

doi

10.1016/0003-9861(85)90627-7

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

277-88

issue

1

eissn

0003-9861

issn

1096-0384

pii

0003-9861(85)90627-7

journal_volume

236

pub_type

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