Homo- and heterodimer formation with prothrombin and prothrombin fragment 1 in the presence of calcium ions.

abstract：:Low-molecular-weight (LMW) thiols are a class of highly reactive compounds massively involved in the maintenance of cellular redox homeostasis. They are implicated in plant responses to almost all stress factors, as well as in the regulation of cellular metabolism. The most studied LMW thiols are glutathione and its b...

journal_title：Archives of biochemistry and biophysics

authors： Pivato M,Fabrega-Prats M,Masi A

更新日期：2014-10-15 00:00:00

abstract：:Sixteen mutants of Escherichia coli defective in H+-ATPase (proton-translocating ATPase) were tested for their ability to recombine with hybrid plasmids carrying various portions of the beta subunit cistron. Twelve mutations were mapped within the carboxyl half of the cistron corresponding to amino acid residues 279 t...

journal_title：Archives of biochemistry and biophysics

authors： Kanazawa H,Noumi T,Oka N,Futai M

更新日期：1983-12-01 00:00:00

abstract：:The manganese-containing (MnSOD) and iron-containing (FeSOD) superoxide dismutases from Escherichia coli are extensively (greater than 95%) inactivated by treatment with phenylglyoxal. The relatively high concentrations of phenylglyoxal and high pH required for optimal inactivation suggest that inactivation may be due...

journal_title：Archives of biochemistry and biophysics

authors： Borders CL Jr,Horton PJ,Beyer WF Jr

更新日期：1989-01-01 00:00:00

abstract：:Phorbol esters induce rapid downregulation of PAF receptors on human neutrophils. The molecular mechanism responsible for this observation has not been elucidated. In the present study, treatment of neutrophils with PMA (10(-7) M) for 10 min caused a complete loss of PAF receptors from the cell surface as determined b...

journal_title：Archives of biochemistry and biophysics

authors： Zhou W,Olson MS

更新日期：1994-08-15 00:00:00

abstract：:Extracellular ATP is known to increase the membrane permeability of a variety of cells. Addition of ATP to human leukemic lymphocytes loaded with the Ca2+ indicator, fura-2, induced a rise in cytosolic Ca2+ concentration which was attenuated or absent in NaCl media compared with KCl, choline Cl, or NMG Cl media. In co...

journal_title：Archives of biochemistry and biophysics

authors： Wiley JS,Chen R,Wiley MJ,Jamieson GP

更新日期：1992-02-01 00:00:00

abstract：:We have examined the effects of calcium and magnesium on both the structural characteristics and the self-association reaction of hyalin, a major protein component of the sea urchin extraembryonic matrix, the hyaline layer. In the absence of calcium, the circular dichroic spectrum revealed a protein possessing a high ...

journal_title：Archives of biochemistry and biophysics

authors： Rimsay R,Robinson JJ

更新日期：2003-06-15 00:00:00

abstract：:Unlike monooxygenases, molybdenum-containing hydroxylases catalyze the hydroxylation of carbon centers using oxygen derived ultimately from water, rather than O(2), as the source of the oxygen atom incorporated into the product, and do not require an external source of reducing equivalents. The mechanism by which this...

journal_title：Archives of biochemistry and biophysics

authors： Hille R

更新日期：2005-01-01 00:00:00

abstract：:Betaine-homocysteine methyltransferase (BHMT) is a member of a family (Pfam 02574) of zinc- and thiol/selenol-dependent methyltransferases. All family members purified to date are monomers, except BHMT, which is an oligomer. We have studied how C-terminal truncation or mutagenic replacement of residues within or assoc...

journal_title：Archives of biochemistry and biophysics

authors： Szegedi SS,Garrow TA

更新日期：2004-06-01 00:00:00

abstract：:We hypothesized that cellular oxygen consumption is abnormal during sepsis as a result of increased oxidative stress and selective mitochondrial damage. In a rat model of sepsis (cecal ligation and puncture), we studied the respiratory characteristics of isolated hepatocytes and liver mitochondria 16 h after onset of ...

journal_title：Archives of biochemistry and biophysics

authors： Kantrow SP,Taylor DE,Carraway MS,Piantadosi CA

更新日期：1997-09-15 00:00:00

abstract：:The secondary structures of staphylococcal nuclease (SNase) have been assigned and semiquantitatively estimated from the deconvoluted Fourier transformed infrared (FTIR) spectrum. The changes in the secondary structures accompanying unfolding and refolding of SNase during reversible thermal denaturation up to 70 degre...

journal_title：Archives of biochemistry and biophysics

authors： Xie L,Jing GZ,Zhou JM

更新日期：1996-04-01 00:00:00

abstract：:Prostacyclin (PGI(2)) synthase is a heme-thiolate (P450) protein which reacts with low levels of peroxynitrite (PN) under tyrosine nitration and inactivation. Studying heme proteins as models, we have found the heme-thiolate protein NADH-NO reductase (P450(NOR)) to be highly efficient in decomposing PN under concomita...

journal_title：Archives of biochemistry and biophysics

authors： Zou MH,Daiber A,Peterson JA,Shoun H,Ullrich V

更新日期：2000-04-01 00:00:00

abstract：:Apigenin is a representative dietary flavone (2-phenyl-4H-1-benzopyran-4-one) inhibiting cancer cell growth both in cell culture systems and in vivo. The prooxidant potential of apigenin was confirmed by the observations using flowcytometric and immunoblotting techniques that the intracellular accumulations of reactiv...

journal_title：Archives of biochemistry and biophysics

authors： Miyoshi N,Naniwa K,Yamada T,Osawa T,Nakamura Y

更新日期：2007-10-15 00:00:00

abstract：:The state of the various, spectroscopically distinguishable copper sites (type 1, type 2, and type 3 copper) of human ceruloplasmin was investigated by electron spin resonance (ESR) spectroscopy. The ESR measurements were performed at 100 K and at X-band during the reaction of the protein with either ascorbate or with...

journal_title：Archives of biochemistry and biophysics

authors： Musci G,Bonaccorsi di Patti MC,Calabrese L

更新日期：1993-10-01 00:00:00

abstract：:Horseradish peroxidase A1 thermal stability was studied by steady-state fluorescence, circular dichroism and differential scanning calorimetry at pH values of 4, 7 and 10. Changes in the intrinsic protein probes, tryptophan fluorescence, secondary structure, and heme group environment are not coincident. The T(m) valu...

journal_title：Archives of biochemistry and biophysics

authors： Carvalho AS,Melo EP,Ferreira BS,Neves-Petersen MT,Petersen SB,Aires-Barros MR

更新日期：2003-07-15 00:00:00

abstract：:Aldehyde oxidase (AO) and xanthine oxidase (XO) are cytosolic enzymes that have been involved in some pathological conditions and play an important role in the biotransformation of drugs and xenobiotics. The increasing interest in these enzymes demands for a simple and rapid procedure for their purification. This pape...

journal_title：Archives of biochemistry and biophysics

authors： Maia L,Mira L

更新日期：2002-04-01 00:00:00

abstract：:Colligin is a collagen-binding glycoprotein localized to the endoplasmic reticulum (ER) and has been proposed to play a role in collagen biosynthesis. Its membership in the serpin family prompted us to examine its effect on procollagen degradation. We first showed that procollagen degradation can take place in the ER ...

journal_title：Archives of biochemistry and biophysics

authors： Jain N,Brickenden A,Ball EH,Sanwal BD

更新日期：1994-10-01 00:00:00

abstract：:Many of the compounds considered for use in pharmaceutical formulations demonstrate incompatibilities with other components at high enough concentrations, including pairs of polymers, polymers and salts, or even proteins in combination with polymers, salts, or other proteins. Freeze concentration can force solutions i...

journal_title：Archives of biochemistry and biophysics

authors： Heller MC,Carpenter JF,Randolph TW

更新日期：1999-03-15 00:00:00

abstract：:Thyroid peroxidase (TPO) was purified from human thyroid tissue, obtained at surgery from patients with Graves' disease, by a procedure similar to one that we had previously used for the purification of porcine TPO. The membrane-bound enzyme was solubilized by treatment of the thyroid particulate fraction with trypsin...

journal_title：Archives of biochemistry and biophysics

authors： Taurog A,Dorris ML,Yokoyama N,Slaughter C

更新日期：1990-05-01 00:00:00

abstract：:Human hemoglobin (Hb) Coimbra (βAsp99Glu) is one of the seven βAsp99 Hb variants described to date. All βAsp99 substitutions result in increased affinity for O2 and decreased heme-heme cooperativity and their carriers are clinically characterized by erythrocytocis, caused by tissue hypoxia. Since βAsp99 plays an impor...

journal_title：Archives of biochemistry and biophysics

authors： Jorge SE,Bringas M,Petruk AA,Arrar M,Marti MA,Skaf MS,Costa FF,Capece L,Sonati MF,Estrin D

更新日期：2018-01-01 00:00:00

abstract：:The amino acid sequences of two very different high-potential iron-sulfur protein (HiPIP) isozymes have been determined from the moderately halophilic purple phototrophic bacterium, Rhodospirillum salinarum. Iso-1 HiPIP, which is monomeric and contains 57 amino acid residues, is most similar to the Thiobacillus ferroo...

journal_title：Archives of biochemistry and biophysics

authors： Ambler RP,Daniel M,Meyer TE,Cusanovich MA

更新日期：1999-09-01 00:00:00

abstract：:The flavoenzyme dihydroorotate dehydrogenase catalyzes the stereoselective oxidation of (S)-dihydroorotate to orotate in the fourth of the six conserved enzymatic reactions involved in the de novo pyrimidine biosynthetic pathway. Inhibition of pyrimidine metabolism by selectively targeting DHODHs has been exploited in...

journal_title：Archives of biochemistry and biophysics

authors： Reis RAG,Calil FA,Feliciano PR,Pinheiro MP,Nonato MC

更新日期：2017-10-15 00:00:00

abstract：:The role of sarcoplasmic reticulum (SR) in malignant hyperthermia (MH) was studied using the heavy microsomal fraction prepared from semitendinosus muscles of both normal and genetically MH-susceptible pigs. In the presence of ATP, SR was loaded with 70 nmol Ca2+/mg SR protein. Under these conditions, MH-SR demonstrat...

journal_title：Archives of biochemistry and biophysics

authors： Ohnishi ST,Waring AJ,Fang SR,Horiuchi K,Flick JL,Sadanaga KK,Ohnishi T

更新日期：1986-06-01 00:00:00

abstract：:Under homeostatic conditions, Nrf2 activity is constitutively repressed. This process is dependent on Keap1, to which Nrf2 binds through the Neh2 domain. Since the N-terminal subdomain of Neh2 (Neh2-NT) contains evolutionarily conserved motifs, we examined the roles they play in the degradation of Nrf2. In Neh2-NT, we...

journal_title：Archives of biochemistry and biophysics

authors： Katoh Y,Iida K,Kang MI,Kobayashi A,Mizukami M,Tong KI,McMahon M,Hayes JD,Itoh K,Yamamoto M

更新日期：2005-01-15 00:00:00

abstract：:An anti-avidin mAb, viz., H12G4, is shown to release bound biotin in a dose-dependent manner from holoavidin and holostreptavidin and inhibit the binding of ligand to the two apoproteins. The release of biotin by this mAb is accompanied by quenching of ligand-induced enhanced fluorescence of the FITC-avidin conjugate....

journal_title：Archives of biochemistry and biophysics

authors： Subramanian N,Subramanian S,Karande AA,Adiga PR

更新日期：1997-08-15 00:00:00

abstract：:Under certain circumstances, added antioxidants can protect tissues against reoxygenation injury after ischemia. Yet reperfusing blood carries many antioxidants with it. The implications of this "antioxidant paradox" are discussed. ...

journal_title：Archives of biochemistry and biophysics

authors： Gutteridge JM,Halliwell B

更新日期：1990-12-01 00:00:00

abstract：:Methylglyoxal (MGO), a reactive dicarbonyl metabolite is a potent arginine directed glycating agent which has implications for diabetes-related complications. Dicarbonyl metabolites are produced endogenously and in a state of misbalance, they contribute to cell and tissue dysfunction through protein and DNA modificati...

journal_title：Archives of biochemistry and biophysics

authors： Abidi M,Khan MS,Ahmad S,Kausar T,Nayeem SM,Islam S,Ali A,Alam K,Moinuddin

更新日期：2018-05-01 00:00:00

abstract：:Tetrahydro-dUMP, an analog of the putative transition state in aminohydrolysis of deoxycytidine monophosphate (dCMP) inhibits the allosteric enzyme deoxycytidylate aminohydrolase with high affinity. The inhibition is reversible, and its kinetics is consistent with the analog binding at the substrate site only to one a...

journal_title：Archives of biochemistry and biophysics

authors： Nucci R,Febbraio F,Piccialli G,de Napoli L,Vaccaro C,Rossi M,Whitehead EP

更新日期：1994-04-01 00:00:00

abstract：:Functional concerns regarding hemoglobin-based red cell substitutes have generally centered on two parameters: (a) oxygen binding and delivery properties and (b) stabilization of the hemoglobin tetramer to prevent dimerization. Strategic chemical cross-linking and site-directed mutagenesis have produced proteins that ...

journal_title：Archives of biochemistry and biophysics

authors： Cashon RE,Alayash AI

更新日期：1995-01-10 00:00:00

abstract：:Phenylalanine ammonia-lyase (PAL) catalyzes the beta-elimination of ammonia from L-phenylalanine to trans-cinnamic acid. A study of inhibition of PAL by phenol, ortho-cresol, and meta-cresol gave mixed inhibition; para-cresol is not an inhibitor. The calculated values of K(i) and alphaK(i) are phenol, K(i)=2.1+/-0.5 m...

journal_title：Archives of biochemistry and biophysics

authors： Alunni S,Cipiciani A,Fioroni G,Ottavi L

更新日期：2003-04-15 00:00:00

abstract：:Bovine kidney mitochondrial extracts contain an inactive protamine kinase and an inactive casein kinase. The protamine kinase was activated by chromatography on poly(L-lysine)-agarose. Two forms of this soluble mitochondrial protamine kinase were separated by chromatography on protamine-agarose. Both forms were purifi...

journal_title：Archives of biochemistry and biophysics

authors： Damuni Z,Reed LJ

更新日期：1988-05-01 00:00:00