A GATA-type transcription factor regulates expression of the high-affinity iron uptake system in the methylotrophic yeast Pichia pastoris.

abstract：:Inactivations of chicken liver pyruvate carboxylase with N-(7-dimethylamino-4-methyl-3-coumarinyl)maleimide (DACM) and o-phthalaldehyde (o-PA) have identified cysteine and lysine residues that are essential for catalytic activity. Protection experiments suggest that the modified residues are located in or near the fir...

journal_title：Archives of biochemistry and biophysics

authors： Werneburg BG,Ash DE

更新日期：1993-06-01 00:00:00

abstract：:Production of hydroxyl radicals (HO.) by substrate-supplemented beef heart submitochondrial particles was studied by electron paramagnetic resonance in conjunction with the spin trap 5,5'-dimethyl-1-pirroline-N-oxide (DMPO). Supplementation of submitochondrial particles with NADH or succinate in the presence of antimy...

journal_title：Archives of biochemistry and biophysics

authors： Giulivi C,Boveris A,Cadenas E

更新日期：1995-02-01 00:00:00

abstract：:Calcium ion is an uncompetitive inhibitor of the inorganic pyrophosphatases of bovine heart and rat liver mitochondria with respect to substrate MgPPi at pH 8.5 and a non-competitive inhibitor of the former enzyme at pH 7.2. The concentration of Ca2+ required to decrease the maximal velocities for both enzymes at pH 8...

journal_title：Archives of biochemistry and biophysics

authors： Baykov AA,Volk SE,Unguryte A

更新日期：1989-09-01 00:00:00

abstract：:Liver cells (HepG2 and primary hepatocytes) overexpressing CYP2E1 and exposed to arachidonic acid (AA) were previously shown to lose viability together with enhanced lipid peroxidation. These events were blocked in cells pre-incubated with antioxidants (alpha-tocopherol, glutathione ethyl ester), or in HepG2 cells not...

journal_title：Archives of biochemistry and biophysics

authors： Caro AA,Cederbaum AI

更新日期：2007-01-15 00:00:00

abstract：:Fructose 2,6-bisphosphate inhibited all three fructose-1,6-bisphosphatases from the liver, intestine, and muscle of the mouse. The sensitivity of the liver enzyme to the inhibitor was significantly diminished when Mg2+ was replaced by Mn2+ as the activating cation. Inhibition of the liver enzyme by fructose 2,6-bispho...

journal_title：Archives of biochemistry and biophysics

authors： Mizunuma H,Tashima Y

更新日期：1983-10-01 00:00:00

abstract：:The phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene. These carotenes are intermediates for the synthesis of spheroidene and spirilloxanthin, respectively. Two different mutation libraries for the crtI gene from R...

journal_title：Archives of biochemistry and biophysics

authors： Stickforth P,Sandmann G

更新日期：2011-01-01 00:00:00

abstract：:Vacuolar H(+)-translocating inorganic pyrophosphatase (V-PPase; EC 3.6.1.1) is a homodimeric proton-translocase; it contains a single type of polypeptide of approximately 81kDa. A line of evidence demonstrated that the carboxyl terminus of V-PPase is relatively conserved in various plant V-PPases and presumably locate...

journal_title：Archives of biochemistry and biophysics

authors： Lin HH,Pan YJ,Hsu SH,Van RC,Hsiao YY,Chen JH,Pan RL

更新日期：2005-10-15 00:00:00

abstract：:Elevation of cytosolic level of Ca(2+) was measured by spatial screening of freshly isolated dorsal root ganglion neurons loaded with Fura-2AM after subjecting them to a moderate hypoxic solution (pO(2)=10-40 mmHg). Short exposure of neurons to hypoxia resulted in a reversible elevation of intracellular Ca(2+) to abou...

journal_title：Archives of biochemistry and biophysics

authors： Lukyanetz EA,Stanika RI,Koval LM,Kostyuk PG

更新日期：2003-02-15 00:00:00

abstract：:Fumarase catalyzes the reversible, stereospecific hydration of fumarate to form L-malate. We have determined the viscosity dependence of V/K and V in both the forward and the reverse directions at pH 6.9 in the absence and presence of several viscosogenic reagents. V/K for fumarate hydration decreases with increasing ...

journal_title：Archives of biochemistry and biophysics

authors： Sweet WL,Blanchard JS

更新日期：1990-02-15 00:00:00

abstract：:Evidence for the formation of a positional isomer of leukotriene (LT) C3 (8,9-LTC3) from dihomo-gamma-linolenic acid has been published (Hammarström, S. J. Biol. Chem. 256, 7712-7714, 1981). This report describes the conversion of dihomo-gamma-linolenic acid to a postulated intermediate in former reaction, 8,9-LTA3, b...

journal_title：Archives of biochemistry and biophysics

authors： Reddy CC,Bertler C,Hammarström S

更新日期：1990-06-01 00:00:00

abstract：:The acute effect of ethanol on hepatic protein synthesis is a rather controversial issue. In view of the conflicting reports on this subject, the effect of ethanol on protein labeling from L-[3H]valine in isolated liver cells was studied under a variety of experimental conditions. When tracer doses of the isotope were...

journal_title：Archives of biochemistry and biophysics

authors： Girbes T,Susin A,Ayuso MS,Parrilla R

更新日期：1983-10-01 00:00:00

abstract：:Superoxide dismutase from the anaerobe Bacteroides fragilis has been purified to apparent homogeneity. The protein, Mr 42,000, is a dimer of equally sized subunits joined by noncovalent interactions. Metal analysis of the native enzyme revealed 1.8-1.9 g-atoms Fe, 0.2 g-atoms Zn, and less than 0.05 g-atoms Mn per mole...

journal_title：Archives of biochemistry and biophysics

authors： Gregory EM,Dapper CH

更新日期：1983-01-01 00:00:00

abstract：:2-Chlorodeoxyadenosine (CldAdo, Cladribine), a nucleoside analog used in the treatment of hairy cell leukemia, is phosphorylated and incorporated into DNA, but is not an absolute chain terminator. We hypothesized that the presence of a chlorine molecule projecting into the DNA minor groove would affect DNA:protein-bin...

journal_title：Archives of biochemistry and biophysics

authors： Hartman WR,Walters DE,Hentosh P

更新日期：2007-03-15 00:00:00

abstract：:The effect of fatty acid binding proteins (FABPs) on two key steps of microsomal phosphatidic acid formation was examined. Rat liver microsomes were purified by size-exclusion chromatography to remove endogenous cytosolic fatty acid and fatty acyl-CoA binding proteins while recombinant FABPs were used to avoid cross-c...

journal_title：Archives of biochemistry and biophysics

authors： Jolly CA,Hubbell T,Behnke WD,Schroeder F

更新日期：1997-05-01 00:00:00

abstract：:Apigenin is a representative dietary flavone (2-phenyl-4H-1-benzopyran-4-one) inhibiting cancer cell growth both in cell culture systems and in vivo. The prooxidant potential of apigenin was confirmed by the observations using flowcytometric and immunoblotting techniques that the intracellular accumulations of reactiv...

journal_title：Archives of biochemistry and biophysics

authors： Miyoshi N,Naniwa K,Yamada T,Osawa T,Nakamura Y

更新日期：2007-10-15 00:00:00

abstract：:Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase [ATP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.49] is completely inactivated by the 2',3'-dialdehyde derivative of ATP (oATP) in the presence of Mn2+. The dependence of the pseudo-first-order rate constant on reagent concentration indicates t...

journal_title：Archives of biochemistry and biophysics

authors： Saavedra C,Araneda S,Cardemil E

更新日期：1988-11-15 00:00:00

abstract：:In cells expressing only the Glut1 isoform of glucose transporters, we have shown that glucose transport is markedly stimulated in response to hypoxia or inhibition of oxidative phosphorylation, conditions that would be expected to cause a stimulation of AMP-activated protein kinase (AMPK) activity. In the present stu...

journal_title：Archives of biochemistry and biophysics

authors： Abbud W,Habinowski S,Zhang JZ,Kendrew J,Elkairi FS,Kemp BE,Witters LA,Ismail-Beigi F

更新日期：2000-08-15 00:00:00

abstract：:The zinc-binding motif (HELLGH) of dipeptidyl peptidase III (DPP III) is different from the common zinc-binding motif (HExxH) of metallopeptidases. To clarify the importance of the zinc-binding motif part of DPP III for enzymatic activity, we measured the recovery of the enzyme activity of apo-Leu(453)-deleted dipepti...

journal_title：Archives of biochemistry and biophysics

authors： Hirose J,Kamigakiuchi H,Iwamoto H,Fujii H,Nakai M,Takenaka M,Kataoka R,Sugahara M,Yamamoto S,Fukasawa KM

更新日期：2004-11-01 00:00:00

abstract：:The use of affinity chromatography on wheat germ agglutinin columns to partially purify detergent extracts of muscarinic acetylcholine receptor from porcine atria is described. The procedure results in a 20-fold purification of the protein. The partially purified protein binds [3H]L-QNB (the L isomer of quinuclidinyl ...

journal_title：Archives of biochemistry and biophysics

authors： Herron GS,Schimerlik MI

更新日期：1984-05-01 00:00:00

abstract：:The alcohol-inducible P450 2E subfamily in the rabbit has two known members that differ in only 16 amino acid residues scattered throughout the polypeptide chain. P450 2E1 has been thoroughly characterized, and is known to have diverse inducers and substrates. Little is known, however, about the properties of P450 2E2...

journal_title：Archives of biochemistry and biophysics

authors： Ding XX,Pernecky SJ,Coon MJ

更新日期：1991-12-01 00:00:00

abstract：:Based on previous findings of increased nitric oxide synthase (NOS) expression in human gliomas (4), we hypothesized that peroxynitrite, a highly reactive metabolite of nitric oxide (NO) and superoxide (O(*-)(2)), might be increased in these tumors in vivo. Here we demonstrate that nitrotyrosine (a footprint of peroxy...

journal_title：Archives of biochemistry and biophysics

authors： Cobbs CS,Samanta M,Harkins LE,Gillespie GY,Merrick BA,MacMillan-Crow LA

更新日期：2001-10-15 00:00:00

abstract：:Benzimidazole compounds, such as omeprazole and thiabendazole, are a different type of CYP1A1 inducer from Ah receptor-ligands, such as TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and 3-methylcholanthrene. In HepG2 cells, the commonly used tyrosine kinase inhibitors, herbimycin-A and a series of tyrphostins, inhibited ...

journal_title：Archives of biochemistry and biophysics

authors： Kikuchi H,Hossain A,Yoshida H,Kobayashi S

更新日期：1998-10-15 00:00:00

abstract：:Glaucoma is the leading cause of irreversible blindness in the world and trabeculectomy remains still the most commonly performed filtration surgery. Failure of trabeculectomy is due to the formation of scarring, which is associated with the increased fibroblast proliferation, activation, and collagen deposition at th...

journal_title：Archives of biochemistry and biophysics

authors： Wang L,Guo D,Wang Z,Yin X,Wei H,Hu W,Chen R,Chen C

更新日期：2020-04-15 00:00:00

abstract：:The reactive cellular products generated during the peroxidation of membrane lipids have been implicated as causative agents in a variety of degenerative diseases and aging. In particular, 4-hydroxynon-2-enal (4HNE) is among the most of the produced during lipid peroxidation. In humans and rodent species, the alpha 4 ...

journal_title：Archives of biochemistry and biophysics

authors： Gardner JL,Gallagher EP

更新日期：2001-06-01 00:00:00

abstract：:For a set of 10 para-substituted toluene derivatives, three enzymatic constants were determined describing their interaction with purified rabbit liver microsomal P-450LM2. The three constants were the catalytic rate constant (Kcat) for hydroxylation, the apparent dissociation constant (Kd) for the enzyme-substrate co...

journal_title：Archives of biochemistry and biophysics

authors： White RE,McCarthy MB

更新日期：1986-04-01 00:00:00

abstract：:An inhibitor protein of the catalytic subunit of the cyclic 3',5'-AMP-dependent protein kinase from the nematode Ascaris suum was isolated and characterized. The molecular weight of the inhibitor was estimated as 28,000 by electrophoresis under denaturing conditions and as 30,000 by gel permeation chromatography on Su...

journal_title：Archives of biochemistry and biophysics

authors： Hoffmann R,Jung S,Hofer HW

更新日期：1992-09-01 00:00:00

abstract：:Nonspecific lipid transfer protein (sterol carrier protein2) has previously been proposed to function as (i) a catalyst for intracellular movement of newly synthesized phospholipid, (ii) a cofactor in the biosynthesis and metabolism of cholesterol, and (iii) a cofactor in the feedback inhibition of cholesterol synthes...

journal_title：Archives of biochemistry and biophysics

authors： Crain RC,Clark RW

更新日期：1985-08-15 00:00:00

abstract：:The effect of high temperature stress on the expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase was examined in wheat (Triticum aestivum L.) leaves, which normally possess 46- and 42-kDa activase forms. Heat stress at 38 degrees C significantly reduced total activase mRNA levels compared ...

journal_title：Archives of biochemistry and biophysics

authors： Law RD,Crafts-Brandner SJ

更新日期：2001-02-15 00:00:00

abstract：:p53, a tumor suppressor and transcription factor, is a critical modulator in the cellular response to stress. Exposure of glutathione-deficient GCS-2 cells to arsenite significantly phosphorylated and stabilized p53. In addition, p53 transcriptionally repressed Hsp90beta gene expression. Mutation analysis revealed a p...

journal_title：Archives of biochemistry and biophysics

authors： Habib GM

更新日期：2009-01-01 00:00:00

abstract：:A protein possessing vascular smooth muscle cell (SMC) growth-promoting activity (VSGP) was purified from bovine ovarian follicular fluid. The purified protein showed a broad band on SDS-PAGE with an apparent molecular mass of 90-100 kDa. The purified protein was characterized by amino acid sequence analysis of its N-...

journal_title：Archives of biochemistry and biophysics

authors： Miyamoto K,Morishita Y,Yamazaki M,Minamino N,Kangawa K,Matsuo H,Mizutani T,Yamada K,Minegishi T

更新日期：2001-06-01 00:00:00