Infrared spectroscopic studies on interactions of water and carbohydrates with a biological membrane.

abstract：:alpha 1-Acid glycoprotein (alpha 1-AGP) is a glucocorticoid inducible gene product that is synthesized and secreted by certain rat hepatoma tissue culture (HTC) cell lines such as M1.54. Exposure to monensin, a Na+-K+ ionophore, causes a significant redistribution of alpha 1-AGP into two distinct fractions; immunoprec...

journal_title：Archives of biochemistry and biophysics

authors： Haffar OK,Edwards CP,Firestone GL

更新日期：1986-04-01 00:00:00

abstract：:Amino acid substitutions at many positions in the a subunit of F1F0 ATP synthase result in impaired proton translocation and altered catalytic activity. In this work, we demonstrate that amino acid substitutions in the a subunit affect the epsilon subunit. In mutant F1F0 ATP synthases, the epsilon subunit was studied ...

journal_title：Archives of biochemistry and biophysics

authors： Gardner JL,Cain BD

更新日期：1999-01-15 00:00:00

abstract：:The so-called 90,000-Da heat shock protein (hsp90) from chicken liver has been purified and physically characterized in the presence of high levels of the serine phosphatase inhibitor fluoride. The protein is an elongated dimer with a molecular weight of 160,000 and a frictional ratio of 1.6. On two-dimensional electr...

journal_title：Archives of biochemistry and biophysics

authors： Iannotti AM,Rabideau DA,Dougherty JJ

更新日期：1988-07-01 00:00:00

abstract：:A cDNA was cloned from Ruta graveolens cells encoding a novel O-methyltransferase (OMT) with high similarity to orcinol or chavicol/eugenol OMTs, but containing a serine-rich N-terminus and a 13 amino acid insertion between motifs IV and V. Expression in Escherichia coli revealed S-adenosyl-l-methionine-dependent OMT ...

journal_title：Archives of biochemistry and biophysics

authors： Burga L,Wellmann F,Lukacin R,Witte S,Schwab W,Schröder J,Matern U

更新日期：2005-08-01 00:00:00

abstract：:A full-length recombinant mutant of diphtheria toxin containing serine in place of a crucial active-site glutamate has been purified and characterized. The serine substitution caused a minor structural alteration in the toxin as measured by trypsinolysis. ADP-ribosyltransferase activity and cytotoxicity of the mutant ...

journal_title：Archives of biochemistry and biophysics

authors： O'Keefe DO

更新日期：1992-08-01 00:00:00

abstract：:An increasing number of studies use calcium-sensitive fluorescent dyes to address the relationship between elevated levels of intracellular calcium and free-radical-mediated damage in a variety of pathophysiological phenomena. The present study evaluates the effects of reactive oxygen species on the spectral propertie...

journal_title：Archives of biochemistry and biophysics

authors： Sarvazyan N,Swift L,Martinez-Zaguilan R

更新日期：1998-02-01 00:00:00

abstract：:Leukotoxin (ltx) and isoleukotoxin (iltx) methyl esters, are metabolites of methyl linoleic acid, an essential fatty acid. They have been associated with acute respiratory distress syndrome. The observed toxicity of ltx and iltx is, in fact, due to the metabolism of the epoxides to their corresponding diols by soluble...

journal_title：Archives of biochemistry and biophysics

authors： Greene JF,Williamson KC,Newman JW,Morisseau C,Hammock BD

更新日期：2000-04-15 00:00:00

abstract：:Rodobacter capsulatus cells, which were cultured anaerobically in high light intensity, had fewer foldings in the cytoplasmic membrane than those which were grown in lower light intensities. Spheroplast-derived membrane fractions obtained from cells cultured under high light intensity contained a high yield of large r...

journal_title：Archives of biochemistry and biophysics

authors： Carmeli C,Lifshitz Y,Friedberg I

更新日期：1991-08-01 00:00:00

abstract：:Lipoxygenases react with hydroperoxy fatty acids and catalyze dioxygenase or dehydrase (leukotriene A4 (LTA4) synthase) types of reactions. In the present investigation we studied the mechanism of reaction of the purified porcine leukocyte 12S-lipoxygenase with 15S-hydroperoxyeicosatetraenoic acid (15S-HPETE). Oxygen-...

journal_title：Archives of biochemistry and biophysics

authors： Brash AR,Yokoyama C,Oates JA,Yamamoto S

更新日期：1989-09-01 00:00:00

abstract：:The in vivo stability of ferredoxin-nitrate reductase from the cyanobacterium Anacystis nidulans under conditions of inhibited protein synthesis has been studied in nitrate-grown cells. A light-promoted rapid decay in cellular nitrate reductase activity took place in the absence of any added nitrogen source, but not i...

journal_title：Archives of biochemistry and biophysics

authors： Herrero A,Flores E,Guerrero MG

更新日期：1984-11-01 00:00:00

abstract：:Iron-sulfur [Fe-S] clusters are ubiquitous ancient prosthetic groups that are required to sustain fundamental life processes. Formation of intracellular [Fe-S] clusters does not occur spontaneously but requires a complex biosynthetic machinery. Different types of [Fe-S] cluster assembly systems have been discovered. A...

journal_title：Archives of biochemistry and biophysics

authors： Fontecave M,Ollagnier-de-Choudens S

更新日期：2008-06-15 00:00:00

abstract：:SH2 domain-containing inositol 5-phosphatase 2 (SHIP2) binds with the Y1356-phosphorylated hepatocyte growth factor (HGF) receptor, c-MET, through its SH2 domain, which is essential for the role of SHIP2 in HGF-induced cell scattering and cell spreading. Previously, the experimental structure of the SH2 domain from SH...

journal_title：Archives of biochemistry and biophysics

authors： Wang Z,Nie Y,Zhang K,Xu H,Ramelot TA,Kennedy MA,Liu M,Zhu J,Yang Y

更新日期：2018-10-15 00:00:00

abstract：:The mouse myoblast cell line C2C12 constitutively expressed 160-kDa transmembrane NCAM isoform and 135-kDa GPI-anchored isoform before differentiation. During differentiation into multinucleated myotubes, the cells newly expressed 150-kDa GPI-anchored isoform and the level of 135-kDa GPI-anchored isoform increased. St...

journal_title：Archives of biochemistry and biophysics

authors： Mukasa R,Umeda M,Endo T,Kobata A,Inoue K

更新日期：1995-04-01 00:00:00

abstract：:The tumor-promoting phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA) inhibited thrombin-stimulated arachidonic acid (AA) release in rabbit and human platelets. PMA was effective over the same concentration range that activates protein kinase C in intact rabbit platelets: IC50 vs thrombin = 0.5 nM, greater th...

journal_title：Archives of biochemistry and biophysics

authors： Murayama T,Kajiyama Y,Takahashi A,Nomura Y

更新日期：1990-01-01 00:00:00

abstract：:Nitric oxide (.NO) and peroxynitrite (ONOO-) inhibit enzymes that depend on metal cofactors or oxidizable amino acids for activity. Since xanthine oxidase (XO) is a 2(2Fe2S) enzyme having essential sulfhydryl groups linked with Mo-pterin cofactor function, the influence of .NO and ONOO- on purified bovine XO was deter...

journal_title：Archives of biochemistry and biophysics

authors： Houston M,Chumley P,Radi R,Rubbo H,Freeman BA

更新日期：1998-07-01 00:00:00

abstract：:L-ribulokinase is unusual among kinases since it phosphorylates all four 2-ketopentoses with almost the same k(cat) values. The K(m)'s differ, however, being 0.14 mM for L- and 0.39 mM for d-ribulose and 3.4 mM for l- and 16 mM for d-xylulose. In addition, L-arabitol is phosphorylated at C-5 (K(m) 4 mM) and ribitol (a...

journal_title：Archives of biochemistry and biophysics

authors： Lee LV,Gerratana B,Cleland WW

更新日期：2001-12-15 00:00:00

abstract：:We have characterized tamulustoxin, a novel 35-amino-acid peptide found in the venom of the Indian red scorpion (Mesobuthus tamulus). Tamulustoxin was identified through a [125I]toxin I screen, designed to identify toxins that block voltage-activated potassium channels. Tamulustoxin has also been cloned by RT-PCR, usi...

journal_title：Archives of biochemistry and biophysics

authors： Strong PN,Clark GS,Armugam A,De-Allie FA,Joseph JS,Yemul V,Deshpande JM,Kamat R,Gadre SV,Gopalakrishnakone P,Kini RM,Owen DG,Jeyaseelan K

更新日期：2001-01-01 00:00:00

abstract：:Diethyl dithiocarbamate (DDC) was used to inhibit the copper- and zinc- containing superoxide dismutase (Cu,ZnSOD) of Escherichia coli in order to expose its physiological function. DDC inhibited the aerobic growth of a sodA sodB mutant much more than it did the growth of a SOD-replete parental strain and this inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Benov L,Fridovich I

更新日期：1996-03-15 00:00:00

abstract：:The nature of the purple complex formed upon the addition of octanoyl-CoA to the medium chain acyl-CoA dehydrogenase from pig kidney has been addressed by chemical quenching studies. Previous work, using quenching in 0.1 M KOH, suggested that the dehydrogenation product, trans-2-octenoyl-CoA, was not a participant in ...

journal_title：Archives of biochemistry and biophysics

authors： Lau SM,Thorpe C

更新日期：1988-04-01 00:00:00

abstract：:Vascular dysfunction and injurious stimuli such as oxidative stress is closely related to the risk of cardiovascular diseases (CVD). Dietary polyphenols is reported to exert the beneficial effects on reducing the risk of CVD. Black soybean is rich in polyphenols, including isoflavones, anthocyanidins and flavan-3-ols,...

journal_title：Archives of biochemistry and biophysics

authors： Yamashita Y,Wang L,Nakamura A,Nanba F,Saito S,Toda T,Nakagawa J,Ashida H

更新日期：2020-07-30 00:00:00

abstract：:Purple Cu(A) centers are a class of binuclear, mixed-valence copper complexes found in cytochrome c oxidase and nitrous oxide reductase. An engineered Cu(A) protein was formed by replacing a portion of the amino acid sequence that contains three of the ligands to the native type I copper center of Pseudomonas aerugino...

journal_title：Archives of biochemistry and biophysics

authors： Sun D,Wang X,Davidson VL

更新日期：2002-08-01 00:00:00

abstract：:Mutagenic substitution of the invariant D42 and D169 residues in phosphoribulokinase (PRK) with amino acids that contain neutral side chains (e.g., alanine or asparagine) results in large decreases in catalytic efficiency (10(5)- and 10(4)-fold for replacement of D42 and D169, respectively). To further evaluate the im...

journal_title：Archives of biochemistry and biophysics

authors： Runquist JA,Miziorko HM

更新日期：2002-09-15 00:00:00

abstract：:Alanine:glyoxylate aminotransferase has been reported to be present as the apo enzyme in the peroxisomes and as the holo enzyme in the mitochondria in chick (white leghorn) embryonic liver. However, surprisingly, birds were found to be classified into two groups on the basis of intraperoxisomal forms of liver alanine:...

journal_title：Archives of biochemistry and biophysics

authors： Sakuraba H,Fujiwara S,Noguchi T

更新日期：1991-05-01 00:00:00

abstract：:Rat Leydig cells in primary culture were used as a model system to investigate the effects of human chorionic gonadotropin (hCG) and dibutyryl cyclic AMP (Bt2cAMP) on the synthesis of cholesterol side chain cleavage cytochrome P-450 (cytochrome P-450scc) and the iron-sulfur protein, adrenodoxin. Leydig cells isolated ...

journal_title：Archives of biochemistry and biophysics

authors： Anderson CM,Mendelson CR

更新日期：1985-05-01 00:00:00

abstract：:Histidine acid phosphatases (HAPs) utilize a nucleophilic histidine residue to catalyze the transfer of a phosphoryl group from phosphomonoesters to water. HAPs function as protein phosphatases and pain suppressors in mammals, are essential for Giardia lamblia excystation, and contribute to virulence of the category A...

journal_title：Archives of biochemistry and biophysics

authors： Dhatwalia R,Singh H,Reilly TJ,Tanner JJ

更新日期：2015-11-01 00:00:00

abstract：:When applied in liquid paraffin to the upper surface of expanding spinach leaves, [1-14C]palmitic acid was efficiently and exclusively incorporated into the sn-1 position of cellular glycerolipids, principally phosphatidylcholine and triacylglycerol. A slow transfer of fatty acids from phosphatidylcholine to chloropla...

journal_title：Archives of biochemistry and biophysics

authors： Roughan PG,Thompson GA Jr,Cho SH

更新日期：1987-12-01 00:00:00

abstract：:Glycophorin from human red blood cells was exposed to ozone in aqueous solution. Amino acid analysis of glycophorin exposed to a 10-fold molar excess of ozone showed that the only residue affected was methionine. Both methionine residues of the protein were oxidized to methionine sulfoxide. Exposure of the oxidized pr...

journal_title：Archives of biochemistry and biophysics

authors： Banerjee SK,Mudd JB

更新日期：1992-05-15 00:00:00

abstract：:Prostacyclin (PGI(2)) synthase is a heme-thiolate (P450) protein which reacts with low levels of peroxynitrite (PN) under tyrosine nitration and inactivation. Studying heme proteins as models, we have found the heme-thiolate protein NADH-NO reductase (P450(NOR)) to be highly efficient in decomposing PN under concomita...

journal_title：Archives of biochemistry and biophysics

authors： Zou MH,Daiber A,Peterson JA,Shoun H,Ullrich V

更新日期：2000-04-01 00:00:00

abstract：:The acute effect of ethanol on hepatic protein synthesis is a rather controversial issue. In view of the conflicting reports on this subject, the effect of ethanol on protein labeling from L-[3H]valine in isolated liver cells was studied under a variety of experimental conditions. When tracer doses of the isotope were...

journal_title：Archives of biochemistry and biophysics

authors： Girbes T,Susin A,Ayuso MS,Parrilla R

更新日期：1983-10-01 00:00:00

abstract：:Under homeostatic conditions, Nrf2 activity is constitutively repressed. This process is dependent on Keap1, to which Nrf2 binds through the Neh2 domain. Since the N-terminal subdomain of Neh2 (Neh2-NT) contains evolutionarily conserved motifs, we examined the roles they play in the degradation of Nrf2. In Neh2-NT, we...

journal_title：Archives of biochemistry and biophysics

authors： Katoh Y,Iida K,Kang MI,Kobayashi A,Mizukami M,Tong KI,McMahon M,Hayes JD,Itoh K,Yamamoto M

更新日期：2005-01-15 00:00:00