Spheroplast-derived membrane vesicles from Rhodobacter capsulatus cells catalyzing nucleotide transport.

Abstract:

:Rodobacter capsulatus cells, which were cultured anaerobically in high light intensity, had fewer foldings in the cytoplasmic membrane than those which were grown in lower light intensities. Spheroplast-derived membrane fractions obtained from cells cultured under high light intensity contained a high yield of large right-side-out membrane vesicles. The right-side-out vesicles catalyzed reversible light-induced proton efflux as did intact cells. Nucleotide transport activity was also catalyzed by these membrane vesicles. This activity was indirectly monitored by measurement of photophosphorylation or hydrolysis of externally added diphospho- and triphosphonucleosides. These enzymatic activities occur inside the cytoplasmic membrane of spheroplasts and membrane vesicles and therefore require the transport of the externally added reagents. The indirect measurements of transport were complemented by the demonstration of direct uptake of radiolabeled nucleotides into the membrane vesicles. These data support the suggestion that a nucleotide transporter located in the cytoplasmic membrane of R. capsulatus bacteria mediates these activities.

journal_name

Arch Biochem Biophys

authors

Carmeli C,Lifshitz Y,Friedberg I

doi

10.1016/0003-9861(91)90229-c

subject

Has Abstract

pub_date

1991-08-01 00:00:00

pages

516-24

issue

2

eissn

0003-9861

issn

1096-0384

pii

0003-9861(91)90229-C

journal_volume

288

pub_type

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