当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Hydrogen peroxide and hypochlorous acid influx through the major S. Typhimurium porin OmpD is affected by substitution of key residues of the channel.

Hydrogen peroxide and hypochlorous acid influx through the major S. Typhimurium porin OmpD is affected by substitution of key residues of the channel.

Abstract：

:OmpD is the major Salmonella enterica serovar Typhimurium (S. Typhimurium) porin and mediates hydrogen peroxide (H2O2) influx. The results described herein extend this finding to hypochlorous acid (HOCl), another reactive oxygen species that is also part of the oxidative burst generated by the phagosome. S. Typhimurium cells lacking OmpD show decreased HOCl influx, and OmpD-reconstituted proteoliposomes show an increase in the uptake of the toxic compound. To understand this physiologically relevant process, we investigated the role of key OmpD residues in H2O2 and NaOCl transport. Using a theoretical approach, residue K16 was defined as a major contributor to the channel electrostatic properties, and E111 was shown to directly participate in the size-exclusion limit of the channel. Together, we provide theoretical, genetic, and biochemical evidence that OmpD mediates H2O2 and NaOCl uptake, and that key residues of the channel are implicated in this process.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Aguayo D,Pacheco N,Morales EH,Collao B,Luraschi R,Cabezas C,Calderón P,González-Nilo F,Gil F,Calderón IL,Saavedra CP

doi

10.1016/j.abb.2015.01.005

subject

Has Abstract

pub_date

2015-02-15 00:00:00

pages

38-45

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(15)00021-1

journal_volume

568

pub_type

杂志文章

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