当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Octameric alcohol oxidase dissociates into stable, soluble monomers upon incubation with dimethylsulfoxide.

Octameric alcohol oxidase dissociates into stable, soluble monomers upon incubation with dimethylsulfoxide.

Abstract：

:Alcohol oxidase (AO) is a peroxisomal, homo-octameric flavoenzyme, which catalyzes methanol oxidation in methylotrophic yeast. Here, we report on the generation of soluble, FAD-lacking AO monomers. Using steady-state fluorescence, fluorescence correlation spectroscopy, circular dichroism and static light scattering approaches, we demonstrate that FAD-lacking AO monomers are formed upon incubation of purified, native octameric AO in a solution containing 50% dimethylsulfoxide (DMSO). Upon removal of DMSO the protein remained monomeric and soluble and did not contain FAD. Binding experiments revealed that the AO monomers bind to purified pyruvate carboxylase, a protein that plays a role in the formation of enzymatically active AO octamers in vivo.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Visser NV,Wang D,Stanley WA,Groves MR,Wilmanns M,Veenhuis M,van der Klei IJ

doi

10.1016/j.abb.2007.01.005

subject

Has Abstract

pub_date

2007-03-15 00:00:00

pages

208-13

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(07)00012-4

journal_volume

459

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • The role of cytochrome c and mitochondrial catalase in hydroperoxide-induced heart mitochondrial lipid peroxidation.

    abstract：:The role of cytochrome c and catalase in hydroperoxide-induced lipid peroxidation of rat heart mitochondria was investigated. Mitoplasts were prepared from hearts of aminotriazole-treated rats which displayed both an 80-90% reduction in matrix catalase activity and rate of H2O2 consumption. Catalase-depleted mitochond...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1993.1055

    authors： Radi R,Bush KM,Freeman BA

    更新日期：1993-01-01 00:00:00

  • The regulatory function of N-terminal AAA+ ATPase domain of eukaryote-like archaeal Orc1/Cdc6 protein during DNA replication initiation.

    abstract：:Archaeal replication machinery represents a core version of this in eukaryotes. The crenarchaeon Sulfolobus solfataricus has the potential to be a powerful model system to understand the central mechanism of eukaryotic DNA replication because it contains three active origins of replication and three eukaryote-like Orc...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.01.007

    authors： He ZG,Feng Y,Wang J,Jiang PX

    更新日期：2008-03-15 00:00:00

  • Induction and superinduction of 2,3,7,8-tetrachlorodibenzo-rho-dioxin-inducible poly(ADP-ribose) polymerase: role of the aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator transcription activation domains and a labile transcription r

    abstract：:The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces a novel poly(ADP-ribose) polymerase (TiPARP). In this study, the signaling pathway of the induction was analyzed. Induction of TiPARP by TCDD occurs in both hepa1c1c7 cells and C57 mouse liver. Induction is concentration and time dependen...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/s0003-9861(02)00339-9

    authors： Ma Q

    更新日期：2002-08-15 00:00:00

  • Comparative effects of cationic triarylmethane, phenoxazine and phenothiazine dyes on horse serum butyrylcholinesterase.

    abstract：:The kinetic effects of a selection of triarylmethane, phenoxazine and phenothiazine dyes (pararosaniline (PR), malachite green (MG), methyl green (MeG); meldola blue (MB), nile blue (NB), nile red (NR); methylene blue (MethB)) and of ethopropazine on horse serum butyrylcholinesterase were studied spectrophotometricall...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.07.006

    authors： Yücel YY,Tacal O,Ozer I

    更新日期：2008-10-15 00:00:00

  • Cloning, tissue distribution, and functional studies of a new cytochrome P450 3A subfamily member, CYP3A45, from rainbow trout (Oncorhynchus mykiss) intestinal ceca.

    abstract：:In trout and mammals, the major extrahepatic expression site for CYP3A forms is in the intestine. A cDNA encoding a new CYP3A subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (RT-PCR), followed by rapid amplification of cDNA ends (RACE)-PCR. In a set o...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/s0003-9861(03)00029-8

    authors： Lee SJ,Buhler DR

    更新日期：2003-04-01 00:00:00

  • Organic hydroperoxide-dependent oxidation of ethanol by microsomes: lack of a role for free hydroxyl radicals.

    abstract：:Organic hydroperoxides can replace NADPH in supporting the oxidation of ethanol by liver microsomes. Experiments were carried out to evaluate the role of hydroxyl radicals in the organic hydroperoxide-catalyzed reaction. Maximum rates of ethanol oxidation occurred in the presence of either 0.5 mM cumene hydroperoxide ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90377-6

    authors： Cederbaum AI

    更新日期：1983-11-01 00:00:00

  • Initiation of rapid, P53-dependent growth arrest in cultured human skin fibroblasts by reactive chlorine species.

    abstract：:Oxidants produced by neutrophils have been implicated in causing cancers associated with chronic inflammation. Hypochlorous acid is the most potent oxidant produced by these cells in appreciable amounts. It reacts with amines to form chloramines, which are weaker oxidants but are mutagenic. Recently, we showed that su...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.1706

    authors： Vile GF,Rothwell LA,Kettle AJ

    更新日期：2000-05-01 00:00:00

  • Myotoxin II from Bothrops asper (Terciopelo) venom is a lysine-49 phospholipase A2.

    abstract：:A basic, dimeric myotoxic protein, myotoxin II, purified from Bothrops asper venom has a similar molecular weight and is immunologically cross-reactive with antibodies raised to previously isolated B. asper phospholipases A2, except that it shows only 0.1% of the phospholipase activity against L-alpha-phosphatidylchol...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90307-5

    authors： Francis B,Gutierrez JM,Lomonte B,Kaiser II

    更新日期：1991-02-01 00:00:00

  • Expression of a plant sesquiterpene cyclase gene in Escherichia coli.

    abstract：:5-Epi-aristolochene synthase is a sesquiterpene cyclase activity found in pathogen-challenged tobacco cells, but not in nonchallenged tissues, and appears to be encoded by a complex gene family. As a prerequisite to assessing the functional significance of these multiple genes, bacterial expression systems were examin...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1533

    authors： Back K,Yin S,Chappell J

    更新日期：1994-12-01 00:00:00

  • Kinetic characterization of the Escherichia coli oligopeptidase A (OpdA) and the role of the Tyr(607) residue.

    abstract：:Oligopeptidase A (OpdA) belongs to the M3A subfamily of bacterial peptidases with catalytic and structural properties similar to mammalian thimet-oligopeptidase (TOP) and neurolysin (NEL). The three enzymes have four conserved Tyr residues on a flexible loop in close proximity to the catalytic site. In OpdA, the flexi...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2010.05.025

    authors： Lorenzon RZ,Cunha CE,Marcondes MF,Machado MF,Juliano MA,Oliveira V,Travassos LR,Paschoalin T,Carmona AK

    更新日期：2010-08-15 00:00:00

  • Kinetic analysis of the binding of Ulex europeas agglutinin 1 (UEA 1) to H-antigenic fucolipid.

    abstract：:The kinetics and thermodynamics of Ulex europeas agglutinin 1-H-antigenic determinant was studied by monitoring the binding of the lectin to H-antigenic fucolipid incorporated in liposomes tethered to the sensor surface by surface plasmon resonance. The second-order rate constants for the interactions are several orde...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1594

    authors： Thomas CJ,Surolia A

    更新日期：2000-02-01 00:00:00

  • Differential effects of natural and synthetic vitamin E on gene transcription in murine T lymphocytes.

    abstract：:Mice were supplemented with low and high doses of natural and synthetic vitamin E, T cells from the spleen isolated and stimulated with plate-bound anti-CD3 and soluble anti-CD28, and gene expression changes assessed by gene array experiments. The data obtained indicate significant qualitative and quantitative differe...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2009.12.015

    authors： Han SN,Pang E,Zingg JM,Meydani SN,Meydani M,Azzi A

    更新日期：2010-03-01 00:00:00

  • Failure of Ah receptor to mediate induction of cytochromes P450 in the CYP1 family in the human hepatoma line SK-Hep-1.

    abstract：:The Ah receptor mediates the induction of cytochrome P450 1A1 (CYP1A1) and toxicities of 2,3,7,8tetrachlorodibanzo-p-dioxin (TCDD). It has been detected in tissues of many species and in murine and human hepatoma lines. We show that the human hepatoma line SK-Hep-1 has cytosolic Ah receptor detectable by specific bind...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.2059

    authors： Roberts EA,Harper PA,Wong JM,Wang Y,Yang S

    更新日期：2000-12-01 00:00:00

  • Regulation of the nitrate reductase level in anacystis nidulans: activity decay under nitrogen stress.

    abstract：:The in vivo stability of ferredoxin-nitrate reductase from the cyanobacterium Anacystis nidulans under conditions of inhibited protein synthesis has been studied in nitrate-grown cells. A light-promoted rapid decay in cellular nitrate reductase activity took place in the absence of any added nitrogen source, but not i...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90292-3

    authors： Herrero A,Flores E,Guerrero MG

    更新日期：1984-11-01 00:00:00

  • Kinetic investigation of the substrate specificity of the cyanogenic beta-D-glucosidase (linamarase) of white clover.

    abstract：:Partially purified linamarase from Trifolium repens (genotype Lili acac) plants was kinetically characterized. Kinetic evidence was found to support the assumption that this cyanogenic beta-D-glucosidase has a broad substrate spectrum. p-Nitrophenyl-beta-D-xylopyranoside and p-nitrophenyl-alpha-L-arabinopyranoside sub...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90245-2

    authors： Pócsi I,Kiss L,Hughes MA,Nánási P

    更新日期：1989-08-01 00:00:00

  • The reduction of putidaredoxin reductase by reduced pyridine nucleotides.

    abstract：:Putidaredoxin reductase (PdR), an FAD-containing protein, mediates the transfer of electrons from NADH to putidaredoxin in the cytochrome P-450cam-dependent oxidation of camphor. Using stopped-flow spectrophotometry, reduction of putidaredoxin reductase by NADH (70 microM) at 4 degrees C appeared to be a pseudo-first-...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90233-0

    authors： Roome PW,Peterson JA

    更新日期：1988-10-01 00:00:00

  • Stark absorption spectroscopy on the carotenoids bound to B800-820 and B800-850 type LH2 complexes from a purple photosynthetic bacterium, Phaeospirillum molischianum strain DSM120.

    abstract：:Stark absorption spectroscopy was applied to clarify the structural differences between carotenoids bound to the B800-820 and B800-850 LH2 complexes from a purple photosynthetic bacterium Phaeospirillum (Phs.) molischianum DSM120. The former complex is produced when the bacteria are grown under stressed conditions of ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2014.12.015

    authors： Horibe T,Qian P,Hunter CN,Hashimoto H

    更新日期：2015-04-15 00:00:00

  • Modulation of acetyl-CoA carboxylase by inhibitors of IMP dehydrogenase: implications for insulin regulation.

    abstract：:The activity of acetyl-CoA carboxylase (ACC), the rate-limiting enzyme of fatty acid biosynthesis, can be regulated by both adenine and guanine nucleotides in vitro. We have employed two inhibitors of IMP dehydrogenase, ribavarin and tiazofurin, to investigate a possible role for intracellular nucleotides in ACC regul...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90016-6

    authors： Witters LA,Mendel DB,Colliton JW

    更新日期：1987-01-01 00:00:00

  • The E117K mutation in β-tropomyosin disturbs concerted conformational changes of actomyosin in muscle fibers.

    abstract：:The effect of the skeletal myopathy-causing E117K mutation in human β-tropomyosin on actomyosin structure during the ATPase cycle was studied using fluorescent probes bound to actin subdomain 1 and the myosin head. Multistep changes in flexural rigidity of actin filament and in spatial arrangement of actin subdomain 1...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2014.03.007

    authors： Karpicheva OE,Redwood CS,Borovikov YS

    更新日期：2014-05-01 00:00:00

  • Papillomavirus binding factor (PBF)-mediated inhibition of cell growth is regulated by 14-3-3beta.

    abstract：:The cellular factor, papillomavirus (PV)-binding factor (PBF)/Huntington's disease binding protein 2 (HDBP2), was identified by its ability to bind regulatory sequences of certain papillomavirus types as well as the Huntington's disease gene. PBF is thought to be a novel nuclear-shuttling transcription factor with unk...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.04.017

    authors： Sichtig N,Silling S,Steger G

    更新日期：2007-08-01 00:00:00

  • Tobacco proliferating cell nuclear antigen binds directly and stimulates both activity and processivity of ddNTP-sensitive mungbean DNA polymerase.

    abstract：:PCNA is well known as a component of DNA replication system and plays important roles in multiple cellular pathways in addition to replication and repair. In this work we have demonstrated the physical and functional interaction between tobacco PCNA and mungbean ddNTP-sensitive DNA polymerase which shares many physico...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.09.009

    authors： Roy S,Roy Choudhury S,Mukherjee SK,Sengupta DN

    更新日期：2007-12-01 00:00:00

  • Heterologous expression and characterization of an active lignin peroxidase from Phanerochaete chrysosporium using recombinant baculovirus.

    abstract：:The cDNA clone lambda ML-1 encoding one of the extracellular lignin peroxidases from the white rot fungus, Phanerochaete chrysosporium, was heterologously expressed in an active form using a recombinant baculovirus system. The glycosylated extracellular form of the recombinant protein contained the ferriprotoporphyrin...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90148-c

    authors： Johnson TM,Li JK

    更新日期：1991-12-01 00:00:00

  • Production of monoclonal antibodies with specificity for different epitopes on the human epidermal growth factor molecule.

    abstract：:Four different monoclonal antibodies (MAbs) to human epidermal growth factor (hEGF) were obtained from mouse hybridoma cell lines. They were highly specific for hEGF and did not cross-react appreciably with mouse EGF. The immunoglobulin class of these MAbs was IgG1 or IgG2a. The apparent Kd values for hEGF of these MA...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90656-x

    authors： Yoshitake Y,Nishikawa K

    更新日期：1988-06-01 00:00:00

  • On the mechanism of action of free purine bases on DNA synthesis in serum-starved L-cells treated with platelet extract.

    abstract：:It has previously been found that there is a synergistic effect of free purine bases and low concentrations of dialyzed platelet extract on net synthesis of DNA in serum-starved fibroblast-like mouse L-cells. Experiments with a mutant line of L-cells that was deficient in hypoxanthine phosphoribosyl transferase (EC 2....

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90699-x

    authors： Flodgaard H,Klenow H

    更新日期：1985-06-01 00:00:00

  • Where is the vitamin D receptor?

    abstract：:The vitamin D receptor (VDR) is a member of the nuclear receptor superfamily and plays a central role in the biological actions of vitamin D. VDR regulates the expression of numerous genes involved in calcium/phosphate homeostasis, cellular proliferation and differentiation, and immune response, largely in a ligand-de...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2012.04.001

    authors： Wang Y,Zhu J,DeLuca HF

    更新日期：2012-07-01 00:00:00

  • Mutations at histidine 211 of the yeast F1-ATPase beta-subunit affect the stability and assembly of the ATPase and the structure of the active site.

    abstract：:The role of H211 of the yeast F1-ATPase beta-subunit was investigated by site-specific mutagenesis and characterization of the resulting enzymes. Five amino acids (N, D, I, K, and A) were substituted for H211 of the ATP2 gene. The mutated genes were expressed in an atp2::LEU2 host, and only yeast expressing H211N resp...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0056

    authors： Schnizer RA,Schuster SM

    更新日期：1996-02-01 00:00:00

  • Androgen receptor-mediated regulation of the anti-atherogenic enzyme CYP27A1 involves the JNK/c-jun pathway.

    abstract：:CYP27A1, an enzyme with several important roles in cholesterol homeostasis and vitamin D₃ metabolism, has been ascribed anti-atherogenic properties. This study addresses an important problem regarding how this enzyme, involved in cholesterol metabolism in the liver and peripheral tissues, is regulated. Our results ide...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2010.11.023

    authors： Norlin M,Pettersson H,Tang W,Wikvall K

    更新日期：2011-02-15 00:00:00

  • Expression and processing of a bacterial endoglucanase in transgenic mice.

    abstract：:The C6.5 endoglucanase from Bacillus subtilis catalyzes the hydrolyses of beta-glucans. This enzyme, which is also produced by many ruminant microbes, is not part of the normal digestive repertoire of monogastric animals. We have generated transgenic mice which express the C6.5 endoglucanase gene specifically in the p...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1243

    authors： Zhang JX,Meidinger R,Forsberg CW,Krell PJ,Phillips JP

    更新日期：1999-07-15 00:00:00

  • Alpha-oxidation of 3-methyl-substituted fatty acids in rat liver.

    abstract：:3-Methyl-substituted fatty acids are first oxidatively decarboxylated (alpha-oxidation) before they are degraded further via beta-oxidation. We synthesized [1-14C]phytanic and 3-[1-14C]methylmargaric acids in order to study their alpha-oxidation in isolated rat hepatocytes, rat liver homogenates and subcellular fracti...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90565-e

    authors： Huang S,Van Veldhoven PP,Vanhoutte F,Parmentier G,Eyssen HJ,Mannaerts GP

    更新日期：1992-07-01 00:00:00

  • Gene structure and expression of the targeting subunit, RGL, of the muscle-specific glycogen-associated type 1 protein phosphatase, PP1G.

    abstract：:The type I phosphatase associated with glycogen, PP1G, plays an important role in glycogen metabolism. PP1G is targeted to glycogen by the R(GL) subunit, which regulates the function of the enzyme. We report the cloning and characterization of the gene as well as the pattern of expression of the R(GL) subunit from mou...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2001.2283

    authors： Lannér C,Suzuki Y,Bi C,Zhang H,Cooper LD,Bowker-Kinley MM,DePaoli-Roach AA

    更新日期：2001-04-01 00:00:00