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Kinetic analysis of the binding of Ulex europeas agglutinin 1 (UEA 1) to H-antigenic fucolipid.

Abstract：

:The kinetics and thermodynamics of Ulex europeas agglutinin 1-H-antigenic determinant was studied by monitoring the binding of the lectin to H-antigenic fucolipid incorporated in liposomes tethered to the sensor surface by surface plasmon resonance. The second-order rate constants for the interactions are several order of magnitude slower than the diffusion controlled reactions. Analyses of activation parameters reveals that these slow second-order rate constants are a consequence of an unfavorable activation enthalpy term.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Thomas CJ,Surolia A

doi

10.1006/abbi.1999.1594

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

8-12

issue

1

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(99)91594-1

journal_volume

374

pub_type

杂志文章

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