Kinetic analysis of the binding of Ulex europeas agglutinin 1 (UEA 1) to H-antigenic fucolipid.

abstract：:The lysosomal cysteine proteinases, cathepsins B, H, and L, were all shown to bind to alpha 2-macroglobulin. The bound enzymes remained active against low-molecular-weight synthetic substrates and bound the active-site-directed inhibitor, benzyloxycarbonyl-125I-Tyr-Ala-diazomethane. Binding of the radiolabeled inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Mason RW

更新日期：1989-09-01 00:00:00

abstract：:Non-enveloped viruses require membrane-penetrating peptides for gaining entry inside the cytoplasm of host cells during the early stages of infection. Although several such peptides have been identified as essential components for non-enveloped virus entry, the molecular mechanism of membrane destabilization by these ...

journal_title：Archives of biochemistry and biophysics

authors： Nair P,Dey D,Borkotoky S,Shukla A,Banerjee M

更新日期：2019-12-15 00:00:00

abstract：:The allylamine class of antifungal compounds are specific inhibitors of squalene epoxidase (SE). However, depending on their chemical structure, allylamine derivatives can be highly selective for either fungal or mammalian SEs. All allylamines tested previously, irrespective of their selectivity, inhibit fungal SEs in...

journal_title：Archives of biochemistry and biophysics

authors： Favre B,Ryder NS

更新日期：1997-04-15 00:00:00

abstract：:In an effort to identify and characterize minor forms of human liver cytochrome P450, immunoblot analyses of microsome samples were developed with antibodies to various P450s that recognized multiple human P450s. Four P450s were recognized in immunoblot analyses of human liver microsome samples developed with an antib...

journal_title：Archives of biochemistry and biophysics

authors： Wrighton SA,Stevens JC,Becker GW,VandenBranden M

更新日期：1993-10-01 00:00:00

abstract：:Cytosolic proteins may play an important role in the intracellular transport of bile acids in enterocytes. The lithocholate binding properties of cytosolic protein from bovine small intestine were studied. Lithocholate binding was observed in the Y (45-50 kDa), Y' (30-35 kDa), and Z fractions (10-15 kDa) following gel...

journal_title：Archives of biochemistry and biophysics

authors： Takikawa H,Arai S,Yamanaka M

更新日期：1992-11-01 00:00:00

abstract：:This work reports the first functional characterization of leishmanial PEPCK. The recombinant Leishmania major enzyme (Lmj_PEPCK) exhibits equivalent kcat values for the phosphoenolpyruvate (PEP) and oxaloacetate (OAA) forming reactions. The apparent Km towards OAA is 10-fold lower than that for PEP, while the Km valu...

journal_title：Archives of biochemistry and biophysics

authors： Sosa MH,Giordana L,Nowicki C

更新日期：2015-10-01 00:00:00

abstract：:This study showcases the potential of unnatural amino acids to enable non-natural functions when incorporated in the protein scaffold of heme metalloproteins. For this purpose, a genetically-engineered myoglobin (Mb) mutant was created by incorporating redox-active 3-amino-l-tyrosine (NH2Tyr) into its active site, rep...

journal_title：Archives of biochemistry and biophysics

authors： Chand S,Ray S,Wanigasekara E,Yadav P,Crawford JA,Armstrong DW,Rajeshwar K,Pierce BS

更新日期：2018-02-01 00:00:00

abstract：:The enzyme phenylalanine hydroxylase catalyzes the hydroxylation of excess phenylalanine in the liver to tyrosine. The enzyme is regulated allosterically by phenylalanine and by phosphorylation of Ser16. Hydrogen/deuterium exchange monitored by mass spectrometry has been used to gain insight into any structural change...

journal_title：Archives of biochemistry and biophysics

authors： Li J,Fitzpatrick PF

更新日期：2013-07-15 00:00:00

abstract：:A green manganese desferrioxamine complex is rapidly formed at room temperature upon stirring freshly precipitated manganese dioxide in a solution of the ligand. Spectral studies and low-temperature ESR indicate that this compound, which has been previously described as a manganese(III) complex, is better characterize...

journal_title：Archives of biochemistry and biophysics

authors： Rush JD,Maskos Z,Koppenol WH

更新日期：1991-08-15 00:00:00

abstract：:Methyl pyruvate was found to exert a dual effect on insulin release from isolated rat pancreatic islets. A positive insulinotropic action prevailed at low concentrations of D-glucose, in the 2.8 to 8.3 mM range, and at concentrations of the ester not exceeding 10.0 mM. It displayed features typical of a process of nut...

journal_title：Archives of biochemistry and biophysics

authors： Malaisse WJ,Jijakli H,Ulusoy S,Cook L,Best L,Viñambres C,Villanueva-Peñacarrillo ML,Valverde I,Sener A

更新日期：1996-11-15 00:00:00

abstract：:We have successfully prepared E- and Z- isomers of 17-20 dehydro analogs of 2-methylene-19-nor-(20S)-1alpha,25-dihydroxyvitamin D3 (2MD). Both isomers bind to the recombinant rat vitamin D receptor (VDR) with high affinity. The Z-isomer (Vit-III 17-20Z) displays activity in vivo and in vitro that is similar to 2MD. Th...

journal_title：Archives of biochemistry and biophysics

authors： Vanhooke JL,Tadi BP,Benning MM,Plum LA,DeLuca HF

更新日期：2007-04-15 00:00:00

abstract：:The glutamine synthetase (GS) gene is expressed at high levels in several cell types, including astrocytes, pericentral hepatocytes, and adipocytes. During hormone-mediated adipocyte differentiation of 3T3-L1 cells, GS gene expression increases several hundred fold. We previously reported that elements in the distal 5...

journal_title：Archives of biochemistry and biophysics

authors： Hadden TJ,Ryou C,Zhu L,Miller RE

更新日期：2002-01-15 00:00:00

abstract：:No major structural alteration of alkaline phosphatase can be observed in the early stages of enzyme oxidative inactivation by the ascorbate model system. Fluorescence changes of protein-bound 8-anilino-1-naphthalenesulfonic acid suggest, however, that localized modifications take place. Oxidized alkaline phosphatase ...

journal_title：Archives of biochemistry and biophysics

authors： Mordente A,Martorana GE,Miggiano GA,Meucci E,Santini SA,Castelli A

更新日期：1988-08-01 00:00:00

abstract：:The changes in retinoid metabolism have been documented in liver cirrhosis. However, the dynamic alterations in levels of this vitamin between circulation and liver during development of the liver cirrhosis are not well understood. The aim of this study was to measure retinoids in the liver and circulation in parallel...

journal_title：Archives of biochemistry and biophysics

authors： Natarajan SK,Thomas S,Ramachandran A,Pulimood AB,Balasubramanian KA

更新日期：2005-11-15 00:00:00

abstract：:A novel bioluminescence-based solid-phase assay is described for the enzyme GDPFuc:Gal(beta)1-3GlcNAc (Fuc to GlcNAc) alpha1,4-fucosyltransferase (alpha1,4FT), which generates the Lewis a blood group antigen (Le(a)) (Gal(beta)1-3[Fuc(alpha)1-4]GlcNAc-R). Lacto-N-tetraose (LNT,Ga ta)1-3GlcNAc(beta)1-3Gal(beta)1-4Glc) w...

journal_title：Archives of biochemistry and biophysics

authors： DeBose-Boyd RA,Nyame AK,Smith DF,Cummings RD

更新日期：1996-11-01 00:00:00

abstract：:The presence of a trifunctional beta-oxidation complex in pig heart and its relationship to the known long-chain enoyl-CoA hydratase (EC 4.2.1.74) from pig heart mitochondria were investigated. For this study, the complex was partially purified by chromatography on DEAE-cellulose in the absence of detergents and was p...

journal_title：Archives of biochemistry and biophysics

authors： Luo MJ,He XY,Sprecher H,Schulz H

更新日期：1993-07-01 00:00:00

abstract：:A novel series of nonpeptidic compounds structurally related to the known anticholesteremic thyropropic acid were found to inhibit Escherichia coli peptide deformylase (PDF), with IC50 values in the low-micromolar range. Kinetic analysis of [4-(4-hydroxyphenoxy)-3,5-diiodophenyl]acetic acid reveals competitive inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Jayasekera MM,Kendall A,Shammas R,Dermyer M,Tomala M,Shapiro MA,Holler TP

更新日期：2000-09-15 00:00:00

abstract：:The nucleotide sequence of tyrosine tRNAQ* psi A from bovine liver was determined to be pC-C-U-U-C-m2G-A-U-A-m2G-C-U-C-A-G-D-D-G-G-acp3U-A-G-A -G-C-m22G-m22G -A-G-G-A-C-U-Q*-psi-A-m1G-A-psi m-C-C-U-U-A-G-m7G-D-m5C-G-C-U-G-G-T-psi-C-G-m1A -U-U-C-C-G-G-C-U-C-G-A-A-G-G-A-C-C-AOH. This tyrosine tRNA is 76 nucleotides in l...

journal_title：Archives of biochemistry and biophysics

authors： Johnson GD,Pirtle IL,Pirtle RM

更新日期：1985-01-01 00:00:00

abstract：:Polycation binding to the negatively charged surface of chloroplast thylakoid membranes is known to cause an inhibition of photosystem I activity. It also interferes with the cation-dependent rearrangement of chlorophyll proteins in the thylakoid membrane. It was shown that added anions prevented or reversed the inhib...

journal_title：Archives of biochemistry and biophysics

authors： Richter ML,Homann PH

更新日期：1983-04-01 00:00:00

abstract：:Lipoxygenase-induced lipid oxidation contributes to plasma lipoprotein oxidation and may be an underlying pathogenic mechanism of atherogenesis. Since inactivation of the vasorelaxant actions of nitric oxide (.NO) plays a critical role in the impaired function of atherosclerotic vessels and because .NO reacts rapidly ...

journal_title：Archives of biochemistry and biophysics

authors： Rubbo H,Parthasarathy S,Barnes S,Kirk M,Kalyanaraman B,Freeman BA

更新日期：1995-12-01 00:00:00

abstract：:Nanomechanical properties of living cells, as measured with atomic force microscopy (AFM), are increasingly recognized as criteria that differentiate normal and pathologically altered cells. Locally measured cell elastic properties, described by the parameter known as Young's modulus, are currently proposed as a new d...

journal_title：Archives of biochemistry and biophysics

authors： Pogoda K,Pięta E,Roman M,Piergies N,Liberda D,Wróbel TP,Janmey PA,Paluszkiewicz C,Kwiatek WM

更新日期：2021-01-15 00:00:00

abstract：:Ischemia/reperfusion injury (IRI) initiates from oxidative stress caused by lack of blood supply and subsequent reperfusion. It is often associated with sterile inflammation, cell death and microvascular dysfunction, which ultimately results in myocardial, cerebral and hepatic IRIs. Reportedly, deregulation of Nrf2 pa...

journal_title：Archives of biochemistry and biophysics

authors： Padmavathi G,Ramkumar KM

更新日期：2021-02-15 00:00:00

abstract：:The conditions for accurately determining distance constraints from TrNOESY data on a small ligand (3'CMP) bound to a small protein (RNase A, <14 kDa) are described. For small proteins, normal TrNOESY conditions of 10:1 ligand:protein or greater can lead to inaccurate structures for the ligand-bound conformation due t...

journal_title：Archives of biochemistry and biophysics

authors： Lee YC,Jackson PL,Jablonsky MJ,Muccio DD

更新日期：2007-07-01 00:00:00

abstract：:Exposure of MCF-7 breast cancer cells to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes an elevated cytochrome P450 content and a marked increase in the microsomal hydroxylation of 17 beta-estradiol (E2) at the C-2, C-4, C-15 alpha, and C-6 alpha positions. In this study we investigated the involvement of cytochrom...

journal_title：Archives of biochemistry and biophysics

authors： Spink DC,Eugster HP,Lincoln DW 2nd,Schuetz JD,Schuetz EG,Johnson JA,Kaminsky LS,Gierthy JF

更新日期：1992-03-01 00:00:00

abstract：:The effects of racemic (+/-) and (+)- and (-)-stereoisomers of isoflurane on depolarization-induced (45)Ca(2+) fluxes mediated by voltage-dependent Ca(2+) channels were investigated in transverse tubule membrane vesicles from rabbit skeletal muscle. In the concentration range 0.5 to 2 mM, (+/-)-isoflurane inhibited (4...

journal_title：Archives of biochemistry and biophysics

authors： Oz M,Tchugunova Y,Dinc M,Dunn SM

更新日期：2002-02-15 00:00:00

abstract：:Primary chondrocyte cell cultures and explants of bovine articular cartilage were subjected to cyclic hydrostatic pressure in a novel computer-controlled pressure chamber designed for this purpose. The cultures were labeled with 5 microCi/ml 35SO4 and simultaneously pressurized with 5 MPa load for 1.5 or 20 h with pre...

journal_title：Archives of biochemistry and biophysics

authors： Parkkinen JJ,Ikonen J,Lammi MJ,Laakkonen J,Tammi M,Helminen HJ

更新日期：1993-01-01 00:00:00

abstract：:Two histone acetyltransferases, A and B, have been extracted and partially purified from yeast cells. The purification scheme included ammonium sulfate precipitation, and chromatography on DEAE-Sepharose and Sephadex G-200. The basic properties of both enzymes closely correspond to those of acetyltransferase A and B f...

journal_title：Archives of biochemistry and biophysics

authors： Lopez-Rodas G,Perez-Ortin JE,Tordera V,Salvador ML,Franco L

更新日期：1985-05-15 00:00:00

abstract：:Prostacyclin (PGI(2)) synthase is a heme-thiolate (P450) protein which reacts with low levels of peroxynitrite (PN) under tyrosine nitration and inactivation. Studying heme proteins as models, we have found the heme-thiolate protein NADH-NO reductase (P450(NOR)) to be highly efficient in decomposing PN under concomita...

journal_title：Archives of biochemistry and biophysics

authors： Zou MH,Daiber A,Peterson JA,Shoun H,Ullrich V

更新日期：2000-04-01 00:00:00

abstract：:A new procedure for the purification of phospholipase C from Clostridium perfringens has been devised that results in essentially pure enzyme. The procedure consists of ammonium sulfate fractionation, ion-exchange chromatography on QAE-Sephadex, and affinity chromatography on phosphatidylcholine linked to Sepharose. T...

journal_title：Archives of biochemistry and biophysics

authors： Krug EL,Kent C

更新日期：1984-06-01 00:00:00

abstract：:The effects of nonlamellar-prone lipids, diacylglycerol and phosphatidylethanolamine (PE), on the kinetic association of SecA with model membranes were examined by measuring changes in the intrinsic emission fluorescence with a stopped-flow apparatus. Upon interaction with standard liposomes composed of 50 mol% dioleo...

journal_title：Archives of biochemistry and biophysics

authors： Ahn T,Kim JS,Lee BC,Yun CH

更新日期：2001-11-01 00:00:00