Abstract:
:The reaction by which the antioxidant capacity of alpha-tocopherol is sustained by recycling of the alpha-tocopheroxyl radical in membranes or lipoproteins by aqueous ascorbate has been studied by laser flash photolysis in model micellar and membrane systems. In bilayers of dimyristoylphosphatidylcholine at 35 degrees C the measured second-order rate constant was 3 x 10(5) M s-1, or about five times slower than previously reported in a solvent system. The rate of reaction was decreased on addition of negatively charged lipid (dipalmitoylphosphatidic acid) and increased by addition of positively charged lipid (didodecyldimethylammonium bromide). These effects of bilayer charge were suppressed by increasing the ionic strength of the aqueous medium. Micellar charge also had an effect on the pH dependence of the reaction rate. Arrhenius data showed that the enthalpy of activation was effectively zero for the reaction in solution between ascorbate and radicals of water-soluble tocopherol analogues, but was positive in membrane and micellar systems. In all cases the entropy of activation was strongly negative. The kinetic deuterium isotope ratio varied between 3 and 8. The data strongly support a concerted electron and proton transfer mechanism for the reaction between alpha-tocopheroxyl radical and ascorbate.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Bisby RH,Parker AWdoi
10.1006/abbi.1995.1150subject
Has Abstractpub_date
1995-02-20 00:00:00pages
170-8issue
1eissn
0003-9861issn
1096-0384pii
S0003-9861(85)71150-2journal_volume
317pub_type
杂志文章abstract::Prostasomes are membranous vesicles (150-200 nm diameter) present in human semen. They are secreted by the prostate gland and contain large amounts of cholesterol, sphingomyelin, and Ca2+. In addition, some of their proteins are enzymes. Prostasomes enhance the motility of ejaculated sperm and are involved in a number...
journal_title:Archives of biochemistry and biophysics
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abstract::Much of what is known about the antioxidant mechanism of vitamin E has been learned from studies of lipid dispersions, solutions, or subcellular organelles. We have investigated the effect of vitamin E supplementation on intact live eucaryotic cells. L1210 murine leukemia cells were exposed to an oxidative stress indu...
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abstract::Rat transferrin (rTf), containing one complex glycan, and human transferrin (hTf), containing two complex glycans, were treated with peptide:N-glycosidase F (PNGase) under nondenaturing conditions. Apo rTf with a nonfucosylated standard biantennary glycan, but not its diferric counterpart, yielded satisfactory amounts...
journal_title:Archives of biochemistry and biophysics
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1996.0144
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journal_title:Archives of biochemistry and biophysics
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更新日期:2018-04-02 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/j.abb.2013.10.009
更新日期:2013-12-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(91)90048-n
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pub_type: 杂志文章
doi:10.1006/abbi.2001.2375
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pub_type: 杂志文章
doi:10.1016/0003-9861(83)90177-7
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pub_type: 杂志文章
doi:10.1016/j.abb.2006.06.002
更新日期:2006-08-01 00:00:00
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doi:10.1006/abbi.1994.1059
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00
abstract::The reduction of duroquinone (DQ), 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone (DB), and dichlorophenol indophenol (DCIP) by succinate and NADH was investigated in yeast mitochondria which have no spectrally detectable cytochrome b. Succinate reduces DB in the cytochrome b-deficient mitochondria at rates comparabl...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(88)90148-8
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abstract::Purified NAD-malic enzyme from Ascaris suum is rapidly inactivated by the arginine reagent, 2,3-butanedione, and this inactivation is facilitated by 30 mM borate. Determination of the inactivation rate as a function of butanedione concentration suggests a second-order process overall, which is first order in butanedio...
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pub_type: 杂志文章
doi:10.1016/0003-9861(87)90288-8
更新日期:1987-05-15 00:00:00
abstract::Adriamycin (AdM) and related anthracyclines are potent antineoplastic agents, the clinical utility of which is limited by severe cardiotoxicity. Aglycone derivatives of AdM have recently been reported to trigger the release of Ca2+ from isolated, preloaded rat heart mitochondria and to modify mitochondrial sulfhydryl ...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:1991-02-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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abstract::A search of the chicken expressed sequence tag (EST) database identified 2 cDNA clones that appeared to represent members of the SULT1B and SULT1C enzyme families. These cDNAs were fully sequenced and found to contain full-length inserts. Phylogenetic analysis of the derived amino acid sequences clearly placed them as...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/j.abb.2004.05.008
更新日期:2004-08-01 00:00:00
abstract::Functional activities of isolated atria from 4-week diabetic rats and their anoxic tolerance and recovery were compared to those of control rats. The present study showed a two-fold increase in maximum developed contraction force (CF), the rate of force development, and the rate of relaxation in isolated atria from di...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(90)90479-i
更新日期:1990-05-15 00:00:00