Testosterone alleviates mitochondrial ROS accumulation and mitochondria-mediated apoptosis in the gastric mucosa of orchiectomized rats.

abstract：:In the presence of glucose and ample oxygen, insect form African trypanosomes release pyruvate more than 100-fold more slowly than do bloodstream forms. This rate decrease could not be accounted for simply by an increased mitochondrial pyruvate oxidation rate as inhibiting mitochondrial respiration increases pyruvate ...

journal_title：Archives of biochemistry and biophysics

authors： Barnard JP,Pedersen PL

更新日期：1994-08-15 00:00:00

abstract：:The soluble ATPase isolated from Streptococcus faecalis membranes containing tightly bound endogenous nucleotides do not exchange in the presence of ATP and Mg+2 added during the purification of the enzyme. In this paper the stoichiometry of endogenous nucleotides in the soluble ATPase obtained from (a) growing cells,...

journal_title：Archives of biochemistry and biophysics

authors： Zlotnick GW,Abrams A

更新日期：1984-05-01 00:00:00

abstract：:A radioimmunoassay was developed to assess the response of testicular HO-1 to agents known to increase the microsomal heme oxygenase activity. Treatment of rats with human chorionic gonadotropin (hCG) increased the microsomal heme oxygenase activity in rat testis. The following data suggest that the increase was speci...

journal_title：Archives of biochemistry and biophysics

authors： Kutty RK,Maines MD

更新日期：1989-01-01 00:00:00

abstract：:Comparative studies of 5-aminoimidazole ribonucleotide (AIR) carboxylases from Escherichia coli and Gallus gallus have identified this central step in de novo purine biosynthesis as a case for unusual divergence in primary metabolism. Recent discoveries establish the fungal AIR carboxylase, encoded by the ADE2 gene, a...

journal_title：Archives of biochemistry and biophysics

authors： Firestine SM,Misialek S,Toffaletti DL,Klem TJ,Perfect JR,Davisson VJ

更新日期：1998-03-01 00:00:00

abstract：:The Ah receptor from rat liver has been purified, using a specific oligonucleotide affinity column, in order to characterize the components of the receptor and to investigate features that modulate its DNA-binding activity. The purified DNA-binding form of rat Ah receptor contains three major components, with estimate...

journal_title：Archives of biochemistry and biophysics

authors： Ireland RC,Li SY,Dougherty JJ

更新日期：1995-06-01 00:00:00

abstract：:Previously human cytochrome P450 3A4 was efficiently and specifically photolabeled by the photoaffinity ligand lapachenole. One of the modification sites was identified as cysteine 98 in the B-C loop region of the protein [B. Wen, C.E. Doneanu, C.A. Gartner, A.G. Roberts, W.M. Atkins, S.D. Nelson, Biochemistry 44 (200...

journal_title：Archives of biochemistry and biophysics

authors： Wen B,Lampe JN,Roberts AG,Atkins WM,David Rodrigues A,Nelson SD

更新日期：2006-10-01 00:00:00

abstract：:Human tissue from uninvolved liver of cancer patients was fractionated using differential centrifugation and characterized for 11betaHSD enzyme activity against corticosterone, dehydrocorticosterone, 7alpha- and 7beta-hydroxy-dehydroepiandrosterone, and 7-oxo-dehydroepiandrosterone. An enzyme activity was observed in ...

journal_title：Archives of biochemistry and biophysics

authors： Robinzon B,Prough RA

更新日期：2009-06-15 00:00:00

abstract：:The purpose of this study was to investigate the effects of thyroid state on rates and sites of H(2)O(2) production in rat muscle mitochondria. With Complex I- and Complex II-linked substrates, hypothyroidism decreased and hyperthyroidism increased the rates of O(2) consumption during State 4 and State 3 respiration a...

journal_title：Archives of biochemistry and biophysics

authors： Venditti P,Puca A,Di Meo S

更新日期：2003-03-01 00:00:00

abstract：:The ferroxidase Fet3 and the permease Ftr1 constitute a well-conserved high-affinity iron uptake system in yeast. We have investigated the mechanism of transcriptional regulation of Fet3 in the methylotrophic yeast Pichia pastoris. Isolation and functional analysis of the Fet3 promoter indicate that a GATA sequence el...

journal_title：Archives of biochemistry and biophysics

authors： Miele R,Barra D,Bonaccorsi di Patti MC

更新日期：2007-09-01 00:00:00

abstract：:Calcium ion is an uncompetitive inhibitor of the inorganic pyrophosphatases of bovine heart and rat liver mitochondria with respect to substrate MgPPi at pH 8.5 and a non-competitive inhibitor of the former enzyme at pH 7.2. The concentration of Ca2+ required to decrease the maximal velocities for both enzymes at pH 8...

journal_title：Archives of biochemistry and biophysics

authors： Baykov AA,Volk SE,Unguryte A

更新日期：1989-09-01 00:00:00

abstract：:An increased permeability of the intestinal barrier is proposed as a major event in the pathophysiology of inflammatory bowel diseases (IBD). Tumor necrosis alpha (TNFα) plays a central role in IBD pathogenesis, in part promoting tight function (TJ) barrier dysfunction. Food extracts enriched in (-)-epicatechin (EC) p...

journal_title：Archives of biochemistry and biophysics

authors： Contreras TC,Ricciardi E,Cremonini E,Oteiza PI

更新日期：2015-05-01 00:00:00

abstract：:Environmental pollution is a major cause of global mortality and burden of disease. All chemical pollution forms together may be responsible for up to 12 million annual excess deaths as estimated by the Lancet Commission on pollution and health as well as the World Health Organization. Ambient air pollution by particu...

journal_title：Archives of biochemistry and biophysics

authors： Daiber A,Kuntic M,Hahad O,Delogu LG,Rohrbach S,Di Lisa F,Schulz R,Münzel T

更新日期：2020-12-15 00:00:00

abstract：:It is widely accepted that the function of human renalase is to oxidize catecholamines in blood. However, this belief is based on experiments that did not account for slow, facile catecholamine autoxidation reactions. Recent evidence has shown that renalase has substrates with which it reacts rapidly. The reaction cat...

journal_title：Archives of biochemistry and biophysics

authors： Beaupre BA,Hoag MR,Moran GR

更新日期：2015-08-01 00:00:00

abstract：:Myeloperoxidase (MPO) is a heme-containing peroxidase expressed mainly in neutrophils and to a lesser degree in monocytes. In the presence of hydrogen peroxide and halides, MPO catalyzes the formation of reactive oxygen intermediates, including hypochlorous acid (HOCl). The MPO/HOCl system plays an important role in m...

journal_title：Archives of biochemistry and biophysics

authors： Aratani Y

更新日期：2018-02-15 00:00:00

abstract：:A comprehensive investigation of peptides derived from the 22-29 region of human islet amyloid polypeptide (hIAPP) that contain phenylalanine analogs at position 23 with a variety of electron donating and withdrawing groups, along with heteroaromatic surrogates, has been employed to interrogate how π-electron distribu...

journal_title：Archives of biochemistry and biophysics

authors： Profit AA,Vedad J,Saleh M,Desamero RZ

更新日期：2015-02-01 00:00:00

abstract：:MAPK-activated protein kinase-2 (MAPKAPK2) regulates the synthesis of tumor necrosis factor and other cytokines and is a potential drug target for inflammatory diseases. Five protein constructs were produced in 4-10mg quantities per liter of culture media using baculovirus-infected insect cells and characterized for k...

journal_title：Archives of biochemistry and biophysics

authors： Kervinen J,Ma H,Bayoumy S,Schubert C,Milligan C,Lewandowski F,Moriarty K,Desjarlais RL,Ramachandren K,Wang H,Harris CA,Grasberger B,Todd M,Springer BA,Deckman I

更新日期：2006-05-15 00:00:00

abstract：:The hydrolysis of 32 X-phenyl-N-methanesulfonyl glycinates by papain was investigated. It was found that the variation in the Michaelis constants could be rationalized by the following correlation equation: log 1/Km = 0.61 pi '3 + 0.46 MR4 + 0.55 sigma + 2.00 with a correlation coefficient of 0.945. In this expression...

journal_title：Archives of biochemistry and biophysics

authors： Carotti A,Smith RN,Wong S,Hansch C,Blaney JM,Langridge R

更新日期：1984-02-15 00:00:00

abstract：:Mutations in MYBPC3, the gene encoding cardiac myosin binding protein C (cMyBP-C), are a major cause of hypertrophic cardiomyopathy (HCM). While most mutations encode premature stop codons, missense mutations causing single amino acid substitutions are also common. Here we investigated effects of a single proline for ...

journal_title：Archives of biochemistry and biophysics

authors： van Dijk SJ,Bezold Kooiker K,Mazzalupo S,Yang Y,Kostyukova AS,Mustacich DJ,Hoye ER,Stern JA,Kittleson MD,Harris SP

更新日期：2016-07-01 00:00:00

abstract：:The expression of plasma membrane Ca2+ pump (PMCA) is regulated by various hormones or agonists via multiple second messenger pathways. Two different 5' segments of the PMCA1 gene (isoform 1) were cloned from a mouse genomic library. While one segment contained the 3' end of intron 1 and exon 2, the other segment was ...

journal_title：Archives of biochemistry and biophysics

authors： Du Y,Carlock L,Kuo TH

更新日期：1995-01-10 00:00:00

abstract：:Complete primary structures of five allergenically active oligosaccharitols (HPG-beta 2-N5a, -N6, -N7a, -N7b, and -N9) derived from a sea squirt H-antigen were studied. Structural characterization was carried out by a new method in which products of limited periodate oxidation, followed by derivatization with p-aminob...

journal_title：Archives of biochemistry and biophysics

authors： Ohta M,Matsuura F,Kobayashi Y,Shigeta S,Ono K,Oka S

更新日期：1991-11-01 00:00:00

abstract：:The present paper focussed on the mechanistic aspect of the reaction of singlet oxygen (1O2), in its lowest excited state (1 Delta g), toward DNA constituents. It is well known that 1O2 is able to react with the guanine moiety of DNA to produce almost specifically 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo). Howev...

journal_title：Archives of biochemistry and biophysics

authors： Ravanat JL,Martinez GR,Medeiros MH,Di Mascio P,Cadet J

更新日期：2004-03-01 00:00:00

abstract：:Rat transferrin (rTf), containing one complex glycan, and human transferrin (hTf), containing two complex glycans, were treated with peptide:N-glycosidase F (PNGase) under nondenaturing conditions. Apo rTf with a nonfucosylated standard biantennary glycan, but not its diferric counterpart, yielded satisfactory amounts...

journal_title：Archives of biochemistry and biophysics

authors： Regoeczi E,Bolyos M,Chindemi PA

更新日期：1989-02-01 00:00:00

abstract：:Glucocorticoids will enhance the growth of cultured human skin fibroblasts in serum-containing medium. In serum-free cultures hydrocortisone (5 X 10(-6) M) will enhance insulin stimulation of sugar transport and DNA synthesis (as measured by thymidine incorporation into trichloroacetic acid-precipitable material). The...

journal_title：Archives of biochemistry and biophysics

authors： Germinario RJ,McQuillan A,Oliveira M,Manuel S

更新日期：1983-10-15 00:00:00

abstract：:1-O-Alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC) induced phosphorylation of two proteins having molecular masses of approximately 20- and 40-kDa in washed rabbit platelets in a concentration- and time-dependent manner. Sequential stimulation with AGEPC did not induce additional protein phosphorylation, supportin...

journal_title：Archives of biochemistry and biophysics

authors： Sugatani J,Hanahan DJ

更新日期：1986-05-01 00:00:00

abstract：:Vanadate V(V) markedly stimulated the oxidation of NADPH by GSSG reductase and this oxidation was accompanied by the consumption of O2 and the accumulation of H2O2. Superoxide dismutases completely eliminated this effect of V(V), whereas catalase was without effect, as was exogenous H2O2 added to 0.1 mM. These effects...

journal_title：Archives of biochemistry and biophysics

authors： Liochev SI,Fridovich I

更新日期：1992-05-01 00:00:00

abstract：:FtsZ (Filamentous temperature sensitivity Z) cell division protein from Escherichia coli binds the fluorescence probe DAPI. Bundling of FtsZ was facilitated in the presence of DAPI, and the polymers in solution remained polymerized longer time than the protofilaments formed in the absence of DAPI. DAPI decreased both ...

journal_title：Archives of biochemistry and biophysics

authors： Nova E,Montecinos F,Brunet JE,Lagos R,Monasterio O

更新日期：2007-09-15 00:00:00

abstract：:Protoporphyrinogen oxidase (EC 1.3.3.4) is the penultimate enzyme in the heme biosynthetic pathway, catalyzing the six-electron oxidation of protoporphyrinogen to protoporphyrin. A dominantly inherited genetic deficiency in this enzyme results in the disease variegate porphyria. We now report the cloning, sequence, an...

journal_title：Archives of biochemistry and biophysics

authors： Dailey TA,Dailey HA,Meissner P,Prasad AR

更新日期：1995-12-20 00:00:00

abstract：:Current issues in research on health effects by polyphenols are addressed. As to the cardiovascular system, flow-mediated dilation (FMD), a functional biomarker, can be used as surrogate marker for cardiovascular risk. Acute short-term effects peaking at 2h after ingestion of polyphenol-rich food items are distinguish...

journal_title：Archives of biochemistry and biophysics

authors： Sies H

更新日期：2010-09-01 00:00:00

abstract：:A soluble monoterpene primary alcohol:NADP+ oxidoreductase has been purified to apparent homogeneity from leaves of the catmint, Nepeta racemosa. The purified enzyme consisted of two polypeptides, with molecular masses of 42,000 and 40,000 Da, and contained zinc ions. A number of monoterpene alcohols (geraniol, nerol,...

journal_title：Archives of biochemistry and biophysics

authors： Hallahan DL,West JM,Wallsgrove RM,Smiley DW,Dawson GW,Pickett JA,Hamilton JG

更新日期：1995-04-01 00:00:00

abstract：:Recent studies have shown that the light-dark mediated regulation of the leaf photosynthetic enzyme pyruvate, Pi dikinase results from interconversion between an active nonphosphorylated form of the enzyme and an inactive form phosphorylated on a threonine residue. These phosphorylation and dephosphorylation reactions...

journal_title：Archives of biochemistry and biophysics

authors： Burnell JN,Hatch MD

更新日期：1986-03-01 00:00:00