Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes.

abstract：:With the concept of precision medicine, combining multiple molecular-targeting therapies has brought new approaches to current cancer treatments. Malfunction of the tumor suppressor protein, p53 is a universal hallmark in human cancers. Under normal conditions, p53 is degraded through an ubiquitin-proteosome pathway r...

journal_title：Archives of biochemistry and biophysics

authors： Chang SJ,Liao EC,Yeo HY,Kuo WH,Chen HY,Tsai YT,Wei YS,Chen YJ,Wang YS,Li JM,Shih CC,Chan CH,Lai ZY,Chou HC,Chuang YJ,Chan HL

更新日期：2018-06-01 00:00:00

abstract：:We have cloned and overexpressed a variant of Arabidopsis thaliana beta-carbonic anhydrase (Q158A) that deletes the functional equivalent of the backbone amide NH of Thr-199 in human alpha-carbonic anhydrase II. The latter residue is hypothesized to be important in catalyzing the rate of CO(2)(-) HCO (3)(-) interconve...

journal_title：Archives of biochemistry and biophysics

authors： Rowlett RS,Tu C,Murray PS,Chamberlin JE

更新日期：2004-05-01 00:00:00

abstract：AIM:We searched for polyphenols capable of inhibiting the lipid accumulation in 3T3-L1 cells, and investigated the mechanisms of two effective chalcones cardamonin and flavokawain B on differentiation of preadipocytes. METHOD AND RESULTS:We treated 3T3-L1 cells with a panel of 46 polyphenols and measured intracellular...

journal_title：Archives of biochemistry and biophysics

authors： Zhang T,Yamamoto N,Yamashita Y,Ashida H

更新日期：2014-07-15 00:00:00

abstract：:Rabbit antibodies have been prepared against ERp61, ERp59, and ERp49, three protein components of rough endoplasmic reticulum (RER) purified from mineral oil-induced plasmacytoma 315 (MOPC-315) tissue. Analysis of subcellular fractions of MOPC-315 tissue by an immunoprecipitation procedure demonstrated that all three ...

journal_title：Archives of biochemistry and biophysics

authors： Lewis MJ,Mazzarella RA,Green M

更新日期：1986-03-01 00:00:00

abstract：:Previous work has shown that inhibitors of protein or mRNA synthesis block endonuclease activation in thymocytes undergoing programmed cell death. In the present study we used isolated nuclei to investigate the effects of cycloheximide and actinomycin D, inhibitors of protein and mRNA synthesis, respectively, on endog...

journal_title：Archives of biochemistry and biophysics

authors： McConkey DJ,Hartzell P,Orrenius S

更新日期：1990-04-01 00:00:00

abstract：:The dioxathiadiaza-heteropentalenes, HEP-I (4,4-dimethyl-1,7-dioxa-2,6-diaza- 7 alpha lambda 4-thia-3H,5H-benzo[cd]pentalene), HEP-II (1,7-dioxa-2, 6-diaza-4, 7 alpha lambda 4-dithia-3H, 5H-benzo[cd]pentalene), HEP-III (1,7-dioxa-2,6-diaza-4, 7 alpha lambda 4-dithia-3H, 5H-benzo[cd]pentalene-4-oxide), and HEP-IV (1,7-...

journal_title：Archives of biochemistry and biophysics

authors： Takahashi M,Nagano T,Hirobe M

更新日期：1989-01-01 00:00:00

abstract：:A cDNA that encodes the human plasminogen (HPg) amino acid sequence has been inserted adjacent to the polyhedrin promoter in the genome of the baculovirus, Autographa californica nuclear polyhedrosis virus, which was then used to infect cultured cells of the farm armyworm, Spodoptera frugiperda. Under the conditions o...

journal_title：Archives of biochemistry and biophysics

authors： Whitefleet-Smith J,Rosen E,McLinden J,Ploplis VA,Fraser MJ,Tomlinson JE,McLean JW,Castellino FJ

更新日期：1989-06-01 00:00:00

abstract：:Resonance Raman spectroscopy (RRS) is a non-invasive method that has been developed to assess carotenoid status in human tissues including human skin in vivo. Skin carotenoid status has been suggested as a promising biomarker for human studies. This manuscript describes research done relevant to the development of thi...

journal_title：Archives of biochemistry and biophysics

authors： Mayne ST,Cartmel B,Scarmo S,Jahns L,Ermakov IV,Gellermann W

更新日期：2013-11-15 00:00:00

abstract：:Hepatocytes from fed rats were incubated for 120 min in the presence of alpha-D-[1,2-13C]glucose pentaacetate (1.7 mM), both D-[1,2-13C]glucose (1.7 mM) and acetate (8.5 mM), alpha-D-glucose penta[2-13C]acetate (1.7 mM), or D-[1,2-13C]glucose (8.3 mM). The amounts of 13C-enriched L-lactate and D-glucose and those of a...

journal_title：Archives of biochemistry and biophysics

authors： Malaisse WJ,Ladrière L,Kadiata MM,Verbruggen I,Willem R

更新日期：2000-09-01 00:00:00

abstract：:The heme enzyme lignin peroxidase contains a unique Cbeta-hydroxylated tryptophan residue (Trp171) on the surface of the enzyme. Mutagenetic substitution of Trp171 abolishes completely the veratryl alcohol oxidation activity of the enzyme. This led us to surmise that Trp171 may be involved in electron transfer from na...

journal_title：Archives of biochemistry and biophysics

authors： Blodig W,Smith AT,Winterhalter K,Piontek K

更新日期：1999-10-01 00:00:00

abstract：:Osmolytes are small organic molecules accumulated by organisms under stress conditions to protect macromolecular structure and function. In the present study, we have investigated the effect of several binary osmolyte mixtures on the protein folding/stability and function of RNase-A. For this, we have measured ΔGD(o) ...

journal_title：Archives of biochemistry and biophysics

authors： Warepam M,Singh LR

更新日期：2015-05-01 00:00:00

abstract：:The extrahepatic expression of UDP-glucuronosyltransferases (UGTs) is important in the detoxification of a number of endogenous and exogenous compounds, including 5-hydroxytryptamine and morphine. Studies were designed to investigate the extrahepatic expression of human UGTs using RT-PCR techniques and to determine th...

journal_title：Archives of biochemistry and biophysics

authors： King CD,Rios GR,Assouline JA,Tephly TR

更新日期：1999-05-01 00:00:00

abstract：:The molecular basis for the unique activities of rabbit cytochrome P450 2B5, compared with the highly related rabbit 2B4, was investigated using hybrid enzymes and site-directed mutagenesis. Alterations in androstenedione hydroxylase profiles observed with 2B4-2B5 hybrids expressed in COS cells showed that key amino a...

journal_title：Archives of biochemistry and biophysics

authors： Szklarz GD,He YQ,Kedzie KM,Halpert JR,Burnett VL

更新日期：1996-03-15 00:00:00

abstract：:A radiochemical assay was developed for measuring branched-chain alpha-ketoacid dehydrogenase activity of Triton X-100 extracts of freeze-clamped rat liver. The proportion of active (dephosphorylated) enzyme was determined by measuring enzyme activities before and after activation of the complex with a broad-specifici...

journal_title：Archives of biochemistry and biophysics

authors： Harris RA,Powell SM,Paxton R,Gillim SE,Nagae H

更新日期：1985-12-01 00:00:00

abstract：:Previously, we reported that emptying of intracellular Ca(2+) pools with endoplasmatic Ca(2+)-ATP-ase inhibitor thapsigargin leads to the Na(+) influx in human lymphocytes (M. Tepel et al., 1994, J. Biol. Chem. 269, 26239-26242). In the present study we examined the mechanism underlying the thapsigargin-induced Na(+) ...

journal_title：Archives of biochemistry and biophysics

authors： Nofer JR,Junker R,Walter M,Seedorf U,Assmann G,Zidek W,Tepel M

更新日期：2000-02-15 00:00:00

abstract：:Thromboxane A2 (TXA) is a potent vasoconstrictor and mediator of platelet aggregation. Thromboxane synthase (TXAS), which catalyzes the biosynthesis of TXA, is a member of the cytochrome P450 superfamily. We report here the complete genomic structure of the human TXAS gene. The gene contains 13 exons and is 193 kb lon...

journal_title：Archives of biochemistry and biophysics

authors： Tazawa R,Green ED,Ohashi K,Wu KK,Wang LH

更新日期：1996-10-15 00:00:00

abstract：:Sendai virus envelopes have been a useful tool in studying the mechanism of membrane-membrane fusion and have served as a vehicle for introducing foreign molecules (e.g., membrane proteins) into recipient cells. Reconstituted Sendai virus envelopes are routinely obtained following solubilization of virus particles wit...

journal_title：Archives of biochemistry and biophysics

authors： Ran S,Nussbaum O,Loyter A,Marikovsky Y,Rivnay B

更新日期：1988-03-01 00:00:00

abstract：:Hemorrhagic toxin f (HT-f) was isolated from Crotalus atrox (Western Diamondback Rattlesnake) venom by a five-step purification procedure. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing, and sodium dodecyl sulfate (SDS)-electrophoresis. HT-f has a ...

journal_title：Archives of biochemistry and biophysics

authors： Nikai T,Mori N,Kishida M,Sugihara H,Tu AT

更新日期：1984-06-01 00:00:00

abstract：:Site-directed mutagenesis was used to probe the role of glycine residue 336 in the regulatory properties of Escherichia coli ADP-glucose pyrophosphorylase. This residue was previously found to be changed from glycine to aspartate in the gene of an Escherichia coli mutant strain. The mutant enzyme had altered kinetic p...

journal_title：Archives of biochemistry and biophysics

authors： Meyer CR,Bork JA,Nadler S,Yirsa J,Preiss J

更新日期：1998-05-01 00:00:00

abstract：:Mammalian mitochondria are sensitive targets of the cytotoxic effects of superoxide (O.2-) and nitric oxide (.NO). In turn, when superoxide and nitric oxide are simultaneously produced, they rapidly react with each other yielding the highly oxidizing peroxynitrite anion (ONOO-) which may be also toxic to mammalian mit...

journal_title：Archives of biochemistry and biophysics

authors： Radi R,Rodriguez M,Castro L,Telleri R

更新日期：1994-01-01 00:00:00

abstract：:Flow cytometry was used to test the role of the propeptide of procathepsin D in the activation of human peripheral lymphocytes and neutrophils. A selective inhibition of the activation was achieved by antibodies targeted against the propeptide. Synthetic peptide corresponding to the cathepsin D propeptide had a very s...

journal_title：Archives of biochemistry and biophysics

authors： Vaclav V,Jana V,Martin F

更新日期：1995-09-10 00:00:00

abstract：:5-Epi-aristolochene synthase is a sesquiterpene cyclase activity found in pathogen-challenged tobacco cells, but not in nonchallenged tissues, and appears to be encoded by a complex gene family. As a prerequisite to assessing the functional significance of these multiple genes, bacterial expression systems were examin...

journal_title：Archives of biochemistry and biophysics

authors： Back K,Yin S,Chappell J

更新日期：1994-12-01 00:00:00

abstract：:The purpose of this review is to summarise the current state of knowledge of (i) the kinetics and mechanisms of radical reactions with carotenoids, (ii) the properties of carotenoid radicals, and (iii) the antioxidant/pro-oxidant properties of carotenoids. ...

journal_title：Archives of biochemistry and biophysics

authors： El-Agamey A,Lowe GM,McGarvey DJ,Mortensen A,Phillip DM,Truscott TG,Young AJ

更新日期：2004-10-01 00:00:00

abstract：:The presence of a trifunctional beta-oxidation complex in pig heart and its relationship to the known long-chain enoyl-CoA hydratase (EC 4.2.1.74) from pig heart mitochondria were investigated. For this study, the complex was partially purified by chromatography on DEAE-cellulose in the absence of detergents and was p...

journal_title：Archives of biochemistry and biophysics

authors： Luo MJ,He XY,Sprecher H,Schulz H

更新日期：1993-07-01 00:00:00

abstract：:The decomposition of S-nitrosoglutathione (GSNO) in the presence of Cu2+ and glutathione (GSH) was studied by stopped-flow/rapid-scan spectroscopy. Reduction of Cu2+ by GSH and subsequent formation of a GS-*Cu+ complex occurred within 200 ms, with the amount of complex formed depending on the GSH-to-Cu2+ ratio. The ra...

journal_title：Archives of biochemistry and biophysics

authors： Gorren AC,Schrammel Astrid,Schmidt K,Mayer B

更新日期：1996-06-15 00:00:00

abstract：:Cofactor and tryptophan accessibility of the 65-kDa form of rat brain glutamate decarboxylase (GAD) was investigated by fluorescence quenching measurements using acrylamide, I-, and Cs+ as the quenchers. Trp residues were partially exposed to solvent. I- was less able and Cs+ was more able to quench the fluorescence o...

journal_title：Archives of biochemistry and biophysics

authors： Rust E,Martin DL,Chen CH

更新日期：2001-08-15 00:00:00

abstract：:The paper reports a study on the reactions of grass pea (Lathyrus sativus) amine oxidase (GPAO) with several aliphatic diamines. The influence of the chain length and of unsaturations in the molecules was examined. Kinetic measurements confirmed that trans-, i.e., (E)-2-butene-1,4-diamine (TDABE) and cis-, i.e., (Z)-2...

journal_title：Archives of biochemistry and biophysics

authors： Sebela M,Frébort I,Lemr K,Brauner F,Pec P

更新日期：2000-12-01 00:00:00

abstract：:The chemical structures of the carotenoid molecules are very simple and one might think that their electronic features are easily predicted. However, there is still has so much unknown information excepting the correlation between the electronic energy state and the length of effective conjugation chain of carotenoids...

journal_title：Archives of biochemistry and biophysics

authors： Uragami C,Saito K,Yoshizawa M,Molnár P,Hashimoto H

更新日期：2018-07-15 00:00:00

abstract：:Amylomaltase (AM) catalyzes inter- and intra-molecular transglycosylation reactions of glucan to yield linear and cyclic oligosaccharide products. The functional roles of the conserved histidine at position 461 in the active site of AM from Corynebacterium glutamicum (CgAM) was investigated. H461 A/S/D/R/W were constr...

journal_title：Archives of biochemistry and biophysics

authors： Tumhom S,Krusong K,Kidokoro SI,Katoh E,Pongsawasdi P

更新日期：2018-08-15 00:00:00

abstract：:Nitric oxide (.NO) and peroxynitrite (ONOO-) inhibit enzymes that depend on metal cofactors or oxidizable amino acids for activity. Since xanthine oxidase (XO) is a 2(2Fe2S) enzyme having essential sulfhydryl groups linked with Mo-pterin cofactor function, the influence of .NO and ONOO- on purified bovine XO was deter...

journal_title：Archives of biochemistry and biophysics

authors： Houston M,Chumley P,Radi R,Rubbo H,Freeman BA

更新日期：1998-07-01 00:00:00