Abstract：
:Previous genetic studies have indicated that the type L alpha-glucan phosphorylase (Pho1) has an essential role during the initiation process of starch biosynthesis during rice seed development. To gain insight into its role in starch metabolism, we characterized the enzymatic properties of the Pho1 recombinant form. Pho1 has significantly higher catalytic efficiency toward both linear and branched alpha-glucans in the synthesis direction than in the degradation direction with equilibrium constants for the various substrates ranging from 13 to 45. Pho1 activity is strongly inhibited by its own reaction product (Pi) in the synthesis reaction (K(i)=0.69 mM) when amylopectin is the primer substrate, but this inhibition is less pronounced (K(i)=14.2 mM) when short alpha-glucan chains are used as primers. Interestingly, even in the presence of Pi alone, Pho1 not only degrades maltohexaose but also extends them to synthesize longer MOSs. Production of a broad spectrum of MOSs (G4-G19) was stimulated by both Pi and Glc1P in an additive fashion. Thus, even under physiological conditions of high Pi/Glc1P, Pho1 extends the chain length of short MOSs which can then be used as subsequent primer by starch synthase activities. As ADP-glucose strongly inhibits Pho1 activity, Pho1 likely operates only during the initial stage and not during maturation phase of starch synthesis.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Hwang SK,Nishi A,Satoh H,Okita TWdoi
10.1016/j.abb.2009.12.023subject
Has Abstractpub_date
2010-03-01 00:00:00pages
82-92issue
1eissn
0003-9861issn
1096-0384pii
S0003-9861(09)00443-3journal_volume
495pub_type
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pub_type： 杂志文章
doi：10.1006/abbi.1995.1359
更新日期：1995-06-20 00:00:00
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pub_type： 杂志文章
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更新日期：2006-04-15 00:00:00
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更新日期：2015-05-15 00:00:00
abstract：:The study aimed to evaluate the impact of adenosine receptors (ARs) on human colon tumor cells (HCT 116, HT-29) growth and sensitivity to 5-Fluorouracil (5-FU) an anticancer chemotherapeutic drug. The exposure of cancer cells to a selective A(3)-AR agonist (IB-MECA) resulted in an increase in HT-29 cells number, where...
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pub_type： 杂志文章
doi：10.1016/j.abb.2013.02.007
更新日期：2013-05-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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更新日期：1983-03-01 00:00:00
abstract：:Type III galactosemia is an inherited disease caused by mutations which affect the activity of UDP-galactose 4'-epimerase (GALE). We evaluated the impact of four disease-associated variants (p.N34S, p.G90E, p.V94M and p.K161N) on the conformational stability and dynamics of GALE. Thermal denaturation studies showed th...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/j.abb.2014.07.030
更新日期：2014-11-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1016/j.abb.2010.12.016
更新日期：2011-04-15 00:00:00
