NADPH-dependent microsomal metabolism of 14,15-epoxyeicosatrienoic acid to diepoxides and epoxyalcohols.

abstract：:Prostaglandin E (PGE) stimulates resorption in bone. Since osteoblast-like osteosarcoma cells secrete PGE2, the possibility that osteoclasts were the major target for PGE was considered. To study this question, it was first established that in isolated bone cells enriched for either osteoclastic (OC) or osteoblastic (...

journal_title：Archives of biochemistry and biophysics

authors： Wong GL,Kocour BA

更新日期：1983-07-01 00:00:00

abstract：:Activation of the NLRP3 inflammasome plays an important role in high glucose- induced endothelial dysfunction in patients with type 2 diabetes mellitus (T2DM). Dulaglutide, a newly developed glucagon-like peptide-1 receptor (GLP-1R) agonist, has been approved for the management of T2DM. In the current study, we aimed ...

journal_title：Archives of biochemistry and biophysics

authors： Luo X,Hu Y,He S,Ye Q,Lv Z,Liu J,Chen X

更新日期：2019-08-15 00:00:00

abstract：:Tritium-labeled 24(R)-hydroxyvitamin D3 and 24(S)-hydroxyvitamin D3 were chemically synthesized and the 1 alpha-hydroxylation of these compounds by chick kidney homogenates was studied. A marked stereospecific preference with regard to the orientation of the hydroxyl functionality on carbon-24 was noted: while the 24(...

journal_title：Archives of biochemistry and biophysics

authors： Ishizuka S,Bannai K,Norman AW

更新日期：1983-09-01 00:00:00

abstract：:Studies were undertaken on the heat denaturation and proteolytic degradation by alpha-chymotrypsin of the normal red cell carbonic anhydrase isozyme, CA II, and two electrophoretic variants of carbonic anhydrase I, CA Ia and CA Ib, of the pigtail macaque. The heat degradation results showed a difference of about 40-fo...

journal_title：Archives of biochemistry and biophysics

authors： Osborne WR,Tashian RE

更新日期：1984-04-01 00:00:00

abstract：:Because angiogenesis underlies the pathogenesis of numerous conditions (cancer, psoriasis, macular degeneration), there is a pressing need for continued investigations into angiogenic signaling and potential drug targets. Antiangiogenic agents can be classified as either direct or indirect. Direct antiangiogenics act ...

journal_title：Archives of biochemistry and biophysics

authors： Berrios RL,Arbiser JL

更新日期：2011-04-15 00:00:00

abstract：:Alanine:glyoxylate aminotransferase has been reported to be present as the apo enzyme in the peroxisomes and as the holo enzyme in the mitochondria in chick (white leghorn) embryonic liver. However, surprisingly, birds were found to be classified into two groups on the basis of intraperoxisomal forms of liver alanine:...

journal_title：Archives of biochemistry and biophysics

authors： Sakuraba H,Fujiwara S,Noguchi T

更新日期：1991-05-01 00:00:00

abstract：:One- and two-dimensional 1H NMR experiments were used to determine the chemical shifts of resonances arising from 29 residues of RNase A in H2O at 17 pH values ranging from 1.2 to 7.9 Nearly all resonances displaying pH-induced changes in chemical shift greater than 0.1 ppm were monitored. Individual plots of the chem...

journal_title：Archives of biochemistry and biophysics

authors： Baker WR,Kintanar A

更新日期：1996-03-01 00:00:00

abstract：:For a set of 10 para-substituted toluene derivatives, three enzymatic constants were determined describing their interaction with purified rabbit liver microsomal P-450LM2. The three constants were the catalytic rate constant (Kcat) for hydroxylation, the apparent dissociation constant (Kd) for the enzyme-substrate co...

journal_title：Archives of biochemistry and biophysics

authors： White RE,McCarthy MB

更新日期：1986-04-01 00:00:00

abstract：:Vanadium salts such as vanadyl sulfate (VS), potent inhibitors of protein tyrosine phosphatases, have been shown to mimic, augment, and prolong insulin's action. However, the molecular mechanism of responses to these salts is not clear. In the present studies, we examined if VS-induced effects on insulin action are as...

journal_title：Archives of biochemistry and biophysics

authors： Théberge JF,Mehdi MZ,Pandey SK,Srivastava AK

更新日期：2003-12-01 00:00:00

abstract：:Physicochemical and saccharide-binding studies have been performed on Trichosanthes cucumerina seed lectin (TCSL). The agglutination activity of TCSL is highest in the pH range 8.0-11.0, whereas below pH 7.0 it decreases quite rapidly, which is consistent with the involvement of imidazole side chains of His residues, ...

journal_title：Archives of biochemistry and biophysics

authors： Kenoth R,Komath SS,Swamy MJ

更新日期：2003-05-01 00:00:00

abstract：:Chlorite dismutase-like proteins are structurally closely related to functional chlorite dismutases which are heme b-dependent oxidoreductases capable of reducing chlorite to chloride with simultaneous production of dioxygen. Chlorite dismutase-like proteins are incapable of performing this reaction and their biologic...

journal_title：Archives of biochemistry and biophysics

authors： Hofbauer S,Hagmüller A,Schaffner I,Mlynek G,Krutzler M,Stadlmayr G,Pirker KF,Obinger C,Daims H,Djinović-Carugo K,Furtmüller PG

更新日期：2015-05-15 00:00:00

abstract：:In the presence of glucose and ample oxygen, insect form African trypanosomes release pyruvate more than 100-fold more slowly than do bloodstream forms. This rate decrease could not be accounted for simply by an increased mitochondrial pyruvate oxidation rate as inhibiting mitochondrial respiration increases pyruvate ...

journal_title：Archives of biochemistry and biophysics

authors： Barnard JP,Pedersen PL

更新日期：1994-08-15 00:00:00

abstract：:Casein kinase 1 phosphorylated rabbit skeletal muscle glycogen synthase at both seryl and threonyl residues. With glycogen synthase phosphorylated up to 7.5 mol phosphate/mol subunit, about 26% of the phosphate was present in the N-terminal cyanogen bromide fragment (CB1) and 74% in the C-terminal fragment (CB2). Both...

journal_title：Archives of biochemistry and biophysics

authors： Guasch MD,Plana M,Vila J,Itarte E

更新日期：1986-11-01 00:00:00

abstract：:There is a current interest in dietary compounds (such as trans-resveratrol) that can inhibit or reverse oxidative stress, the common pathway for a variety of brain disorders, including Alzheimer's disease and stroke. The objective of the present study was to investigate the effects of resveratrol, under conditions of...

journal_title：Archives of biochemistry and biophysics

authors： de Almeida LM,Leite MC,Thomazi AP,Battu C,Nardin P,Tortorelli LS,Zanotto C,Posser T,Wofchuk ST,Leal RB,Gonçalves CA,Gottfried C

更新日期：2008-12-01 00:00:00

abstract：:Affinity columns prepared by immobilizing monoclonal antibodies that specifically recognize the Lea or the Leb blood group antigens can be used for analytical or preparative isolation of oligosaccharides with the corresponding reactivities. The number of immobilized functional antibody combining sites on a column and ...

journal_title：Archives of biochemistry and biophysics

authors： Dakour J,Lundblad A,Zopf D

更新日期：1988-07-01 00:00:00

abstract：:Autophagy, a ubiquitous degradation pathway, is important for the survival and homeostasis of cells. Previous studies have demonstrated the role of autophagy in host defense against bacterial infection, but the importance of autophagy in the intestinal epithelium for the regulation of bacterial infection has not been ...

journal_title：Archives of biochemistry and biophysics

authors： Inoue J,Nishiumi S,Fujishima Y,Masuda A,Shiomi H,Yamamoto K,Nishida M,Azuma T,Yoshida M

更新日期：2012-05-01 00:00:00

abstract：:A cyanide-insensitive superoxide dismutase was purified to electrophoretic homogeneity from leaves of the eucaryote, Ginkgo biloba L. A molecular mass of 47,000 was determined for the enzyme, which consisted of two subunits of equal size. The enzyme preparation contained two isoenzymes with isoelectric points of 5.25 ...

journal_title：Archives of biochemistry and biophysics

authors： Duke MV,Salin ML

更新日期：1985-11-15 00:00:00

abstract：:Substrate specificity of human mitochondrial low Km aldehyde dehydrogenase (EC 1.2.1.3) E2 isozyme has been investigated employing p-nitrophenyl esters of acyl groups of two to six carbon atoms and comparing with that of aldehydes of one to eight carbon atoms. The esterase reaction was studied under three conditions: ...

journal_title：Archives of biochemistry and biophysics

authors： Mukerjee N,Pietruszko R

更新日期：1992-11-15 00:00:00

abstract：:Cancer osaka thyroid (COT), a human MAP 3 K, is essential for lipopolysaccharide activation of the Erk MAPK cascade in macrophages. COT 30--467 is insoluble, whereas low levels of COT 30--397 can be expressed, but this protein is unstable. However, both COT 30--467 and COT 30--397 are expressed in a soluble and stable...

journal_title：Archives of biochemistry and biophysics

authors： Jia Y,Quinn CM,Bump NJ,Clark KM,Clabbers A,Hardman J,Gagnon A,Kamens J,Tomlinson MJ,Wishart N,Allen H

更新日期：2005-09-01 00:00:00

abstract：:Properly regulated cyclin proteolysis is critical for normal cell cycle progression. A nine-amino acid peptide motif called the destruction box (D box) is present at the N terminus of the yeast mitotic cyclins. This short sequence is required for cyclin ubiquitination and subsequent proteolysis. The anaphase-promoting...

journal_title：Archives of biochemistry and biophysics

authors： Meyn MA 3rd,Melloy PG,Li J,Holloway SL

更新日期：2002-11-15 00:00:00

abstract：:In order to characterize cellular retinoic acid-binding protein (CRABP) from chick embryos, we resolved the [3H]RA-binding activity of embryo extracts by Mono Q anion-exchange chromatography. Fractions containing [3H]RA-binding proteins eluted in two peaks at 7 and 10 min with the latter showing 10-fold higher activit...

journal_title：Archives of biochemistry and biophysics

authors： Venepally P,Reddy LG,Sani BP

更新日期：1996-12-15 00:00:00

abstract：:The epithelial cells of the gastrointestinal tract have the capacity to engage in biotransformation of ingested chemicals. A principal component of phase I metabolism of xenobiotics is the family of hemeproteins referred to as cytochrome(s) P450. The presence of cytochrome P450 isoforms was examined by Western blot an...

journal_title：Archives of biochemistry and biophysics

authors： Rosenberg DW

更新日期：1991-01-01 00:00:00

abstract：:Phenylalanine ammonia-lyase (PAL) catalyzes the beta-elimination of ammonia from L-phenylalanine to trans-cinnamic acid. A study of inhibition of PAL by phenol, ortho-cresol, and meta-cresol gave mixed inhibition; para-cresol is not an inhibitor. The calculated values of K(i) and alphaK(i) are phenol, K(i)=2.1+/-0.5 m...

journal_title：Archives of biochemistry and biophysics

authors： Alunni S,Cipiciani A,Fioroni G,Ottavi L

更新日期：2003-04-15 00:00:00

abstract：:The solution structure of native and systematically modified ovine submaxillary mucin (OSM) has been probed by proton NMR spectroscopic methods. Most of the resonances in the spectra have been tentatively assigned to the peptide and O-linked disaccharide, alpha-N-acetylneuraminic acid 2----6 alpha-N-acetylgalactosamin...

journal_title：Archives of biochemistry and biophysics

authors： Gerken TA

更新日期：1986-06-01 00:00:00

abstract：:Superoxide generation, assessed as the rate of acetylated cytochrome c reduction inhibited by superoxide dismutase, by purified NADPH cytochrome P-450 reductase or intact rat liver microsomes was found to account for only a small fraction of their respective NADPH oxidase activities. DTPA-Fe3+ and EDTA-FE3+ greatly st...

journal_title：Archives of biochemistry and biophysics

authors： Morehouse LA,Thomas CE,Aust SD

更新日期：1984-07-01 00:00:00

abstract：:The rotating frame nuclear magnetic resonance relaxation rate R(1rho) in the blood and cell lysate was studied at 4.7T to provide reference values for in vivo modeling and to address the mechanisms contributing to net relaxation. A strong dependence on oxygenation, hematocrit, and spin lock field strength B(1) (0.2-1....

journal_title：Archives of biochemistry and biophysics

authors： Johanna Silvennoinen M,Kettunen MI,Clingman CS,Kauppinen RA

更新日期：2002-09-01 00:00:00

abstract：:Conversion of geranyl pyrophosphate to cyclic monoterpenes is considered to involve the preliminary isomerization of this acyclic precursor to enzyme-bound linalyl pyrophosphate and the cyclization of this tertiary intermediate. 2-Fluorogeranyl pyrophosphate and 2-fluorolinalyl pyrophosphate are effective competitive ...

journal_title：Archives of biochemistry and biophysics

authors： Croteau R

更新日期：1986-12-01 00:00:00

abstract：:The reaction of phospholipid hydroperoxide glutathione peroxidase (PHGPx) and Ebselen with phospholipid and cholesterylester hydroperoxides associated with HDLox and LDLox was investigated using specific HPLC assays for the hydroperoxides of phosphatidylcholine (PCOOH) and cholesteryllinolate (Ch18:2-OOH) and for chol...

journal_title：Archives of biochemistry and biophysics

authors： Sattler W,Maiorino M,Stocker R

更新日期：1994-03-01 00:00:00

abstract：:Inactivation of the von Hippel-Lindau (VHL) tumor suppressor is associated with renal carcinoma, hemangioblastoma and pheochromocytoma. The VHL protein is a component of a ubiquitin ligase complex that ubiquitinates and degrades hypoxia inducible factor-α (HIF-α). Degradation of HIF-α by VHL is proposed to suppress tu...

journal_title：Archives of biochemistry and biophysics

authors： Lai Y,Song M,Hakala K,Weintraub ST,Shiio Y

更新日期：2012-02-15 00:00:00

abstract：:We have developed derivatives of mouse embryonic fibroblasts (10T1/2) and Chinese hamster ovary (AS52) cells that stably express high levels of murine prostaglandin synthase-1 or -2 (PGHS-1 or -2). The cDNAs were transferred using retroviral vectors and the resulting G418-resistant clones were analyzed for prostagland...

journal_title：Archives of biochemistry and biophysics

authors： Chulada PC,Loftin CD,Winn VD,Young DA,Tiano HF,Eling TE,Langenbach R

更新日期：1996-06-15 00:00:00