Abstract:
:A cyanide-insensitive superoxide dismutase was purified to electrophoretic homogeneity from leaves of the eucaryote, Ginkgo biloba L. A molecular mass of 47,000 was determined for the enzyme, which consisted of two subunits of equal size. The enzyme preparation contained two isoenzymes with isoelectric points of 5.25 and 5.15. Metal analysis after dialysis against EDTA revealed the presence of 1.4 gram atoms of iron per molecule. Approximately 2 gram atoms each of copper and zinc per enzyme molecule were also detected, although removal of copper by other chelators had no effect on enzymatic activity. The purified Ginkgo enzyme exhibited a sensitivity to hydrogen peroxide and insensitivity to cyanide, which is typical of iron-containing superoxide dismutases. Ginkgo iron superoxide dismutase was localized in the stroma of chloroplasts, but was absent from mitochondria. The enzyme from Ginkgo was most similar to iron superoxide dismutases of Nuphar, Brassica, and Escherichia coli when compared on the basis of S delta Q analysis of amino acid composition. Peptide fragments formed by proteolytic digestion of these four enzymes were compared qualitatively; similar-sized fragments which denote possible areas of homology were observed.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Duke MV,Salin MLdoi
10.1016/0003-9861(85)90800-8subject
Has Abstractpub_date
1985-11-15 00:00:00pages
305-14issue
1eissn
0003-9861issn
1096-0384pii
0003-9861(85)90800-8journal_volume
243pub_type
杂志文章abstract::Binding sites having the properties of high-affinity receptors for activated alpha 2-macroglobulin (alpha 2M) have been purified over 100-fold from membranes of spontaneously transformed NIH-3T3 cells (J. A. Hanover, S.-y. Cheng, M. C. Willingham, and I. H. Pastan [1983] J. Biol. Chem. 258, 370-377). To identify the m...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(83)90486-1
更新日期:1983-12-01 00:00:00
abstract::The cytosol fraction of an extract of Xenopus laevis ovaries contains a protein inhibitor that can specifically block the activation of calmodulin-sensitive cyclic nucleotide phosphodiesterase (PDE I) found in that tissue. This inhibitor was purified by DEAE-cellulose chromatography, gel filtration on Sephacryl S-200,...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(85)90377-7
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(90)90278-7
更新日期:1990-05-01 00:00:00
abstract::Four different monoclonal antibodies (MAbs) to human epidermal growth factor (hEGF) were obtained from mouse hybridoma cell lines. They were highly specific for hEGF and did not cross-react appreciably with mouse EGF. The immunoglobulin class of these MAbs was IgG1 or IgG2a. The apparent Kd values for hEGF of these MA...
journal_title:Archives of biochemistry and biophysics
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更新日期:1988-06-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:1992-01-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
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更新日期:1993-03-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
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doi:10.1006/abbi.1993.1062
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(90)90426-y
更新日期:1990-08-15 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1998.0704
更新日期:1998-07-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
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doi:10.1016/0003-9861(90)90753-l
更新日期:1990-02-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
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doi:10.1016/0003-9861(87)90059-2
更新日期:1987-02-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/j.abb.2007.06.013
更新日期:2007-09-15 00:00:00
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journal_title:Archives of biochemistry and biophysics
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更新日期:2016-01-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
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doi:10.1006/abbi.1995.1111
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更新日期:1994-11-15 00:00:00
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journal_title:Archives of biochemistry and biophysics
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doi:10.1006/abbi.1997.0264
更新日期:1997-09-15 00:00:00
abstract::In the absence of NADPH, the addition of an arachidonic acid hydroperoxide, 15-hydroperoxyeicosa-5,8,11,13-tetraenoic acid, to liver microsomes, prepared from phenobarbital-treated rats, resulted in the formation of two major metabolites and several minor products, some of which have been purified by reverse-phase hig...
journal_title:Archives of biochemistry and biophysics
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更新日期:1987-01-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1999.1594
更新日期:2000-02-01 00:00:00
abstract::Two isozymes of catalase (EC 1.11.1.6), one with typically low peroxidatic activity (CAT-1) and the other with enhanced-peroxidatic activity (EP-CAT or CAT-3) have been purified to electrophoretic homogeneity from tobacco (Nicotiana sylvestris) seedlings and antibodies prepared against each. The isozyme proteins showe...
journal_title:Archives of biochemistry and biophysics
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更新日期:1990-12-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
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更新日期:2002-09-15 00:00:00
abstract::Isoniazid (INH) is one of the oldest drugs for the treatment of tuberculosis (TB) and is of continual clinical and research interest. The aim of the current study is to investigate the ability of INH to induce monocyte differentiation and the underlying signaling pathway involved in this phenomenon using HL-60 cells. ...
journal_title:Archives of biochemistry and biophysics
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更新日期:2019-03-30 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章,评审
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更新日期:2018-02-15 00:00:00
abstract::Troponin T (TnT) binds to tropomyosin (Tm) to anchor the troponin complex in the thin filament, and it thus serves as a vital link in the Ca(2+) regulation of striated muscle contraction. Pioneer work three decades ago determined that the T1 and T2 chymotryptic fragments of TnT each contains a Tm-binding site. A more ...
journal_title:Archives of biochemistry and biophysics
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更新日期:2010-08-15 00:00:00
abstract::Endometrial carcinoma is a type of gynecological cancer that originates in the endometrial epithelial tissue. Due to its high proliferation and ability to invade muscle tissue, it is one of the most common malignant tumors in the female reproductive system. Fatostatin is a small molecule non-sterol diarylthiazole deri...
journal_title:Archives of biochemistry and biophysics
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更新日期:2020-05-15 00:00:00
abstract::The flavoprotein monoamine oxidase B (MAO B) from bovine liver, as isolated, has an unusual additional absorption band at 412 nm, which is similar to the absorption of its anionic flavin semiquinone form, (Fl.-), and other typical (Fl.-) flavoproteins. Denaturation of the enzyme results in the elimination of this anom...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1993.1025
更新日期:1993-01-01 00:00:00