Intramolecular cross-linking in the native JHBP molecule.

abstract：:Serratia marcescens metalloprotease inhibitor (SmaPI) is a proteinase inhibitor toward Serratia marcescens metalloprotease (SMP). In sequential deletion analysis of the N-terminal region of the SmaPI, SmaPIs starting at Ser-2 and Leu-3 residues, respectively, had nearly a full inhibitory activity toward SMP. However, ...

journal_title：Archives of biochemistry and biophysics

authors： Bae KH,Kim IC,Kim KS,Shin YC,Byun SM

更新日期：1998-04-01 00:00:00

abstract：:The binding site for ATP to tubulin was established by use of the photoaffinity label [gamma-32P]N3ATP. Photolysis of the analog in the presence of tubulin resulted in covalent modification of the protein as revealed by autoradiography of electropherograms. Scanning the autoradiograms showed that the ATP analog was bo...

journal_title：Archives of biochemistry and biophysics

authors： Zabrecky JR,Cole RD

更新日期：1983-09-01 00:00:00

abstract：:Fibrocartilage is found in tendon at sites where the tissue is subjected to transverse compressive loading in vivo. A significant characteristic of the tissue transition from tendon to fibrocartilage in bovine deep flexor tendon is increased gene expression, synthesis, and accumulation of both a large proteoglycan, ag...

journal_title：Archives of biochemistry and biophysics

authors： Robbins JR,Evanko SP,Vogel KG

更新日期：1997-06-15 00:00:00

abstract：:MicroRNA-342-3p (miR-342) has been shown to act as a tumor-suppressor in different cancer types. However, the role and therapeutic implications of miR-342 via modulation of Cofilin 1 (CFL1) has not been studied in any type of cancer. Given the importance of Cofilin signalling in breast, this study was undertaken to ex...

journal_title：Archives of biochemistry and biophysics

authors： Liu C,Xing H,Luo X,Wang Y

更新日期：2020-07-15 00:00:00

abstract：:The protein Mop from Haemophilus influenzae is a member of the molbindin family of proteins. Using isothermal titration calorimetry (ITC), Mop was observed to bind molybdate at two distinct sites with a stoichiometry of 8 mol molybdate per Mop hexamer. Six moles of molybdate bound endothermically at high affinity site...

journal_title：Archives of biochemistry and biophysics

authors： Masters SL,Howlett GJ,Pau RN

更新日期：2005-07-01 00:00:00

abstract：:Reactive oxygen species (ROS) can be generated in experimental shock states through several different mechanisms. We measured ROS production in metabolically active liver mitochondria from rats rendered septic by cecal ligation and puncture. By polarography, the State 4 and State 3 respiration rates of liver mitochond...

journal_title：Archives of biochemistry and biophysics

authors： Taylor DE,Ghio AJ,Piantadosi CA

更新日期：1995-01-10 00:00:00

abstract：:Ochratoxin A, a nephrotoxin produced by Aspergillus ochraceus, decreases the activity of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) (PEPCK) in the cytosol of rat kidneys, as well as inhibits renal gluconeogenesis (Meisner, H., and Meisner, P. (1981) Arch. Biochem. Biophys. 208, 146-153). Ochratoxin A greatl...

journal_title：Archives of biochemistry and biophysics

authors： Meisner H,Cimbala MA,Hanson RW

更新日期：1983-05-01 00:00:00

abstract：:A58 is a murine monoclonal antibody raised against the internal dimeric subunit A alpha 1 of the hemocyanin of the scorpion Androctonus australis. When A58 is tested against a range of related hemocyanins, it exhibits cross-reactivity with a limited number of scorpion species. All of them belong to a single Scorpion F...

journal_title：Archives of biochemistry and biophysics

authors： Billiald P,Motta G,Vaux DJ

更新日期：1995-03-10 00:00:00

abstract：:Role of c-Src in muscle differentiation has been controversial. Here, we investigated if c-Src positively or negatively regulates muscle differentiation, using H9c2 and C2C12 cell lines. Inhibition of c-Src by treatment with PP1 and SU6656, pharmacologic inhibitors of Src family kinases, or by expression of a dominant...

journal_title：Archives of biochemistry and biophysics

authors： Lim MJ,Seo YH,Choi KJ,Cho CH,Kim BS,Kim YH,Lee J,Lee H,Jung CY,Ha J,Kang I,Kim SS

更新日期：2007-09-01 00:00:00

abstract：:The interactions of Cu, Zn superoxide dismutase (SOD) with nitroxyl (NO-) and nitric oxide (NO), both of which are thought to be biologically significant, have been studied but remain undefined. Having previously noted that NO- can reduce Cu (II), Zn SOD aerobically, we now report that it also can do so anaerobically ...

journal_title：Archives of biochemistry and biophysics

authors： Liochev SI,Fridovich I

更新日期：2002-06-15 00:00:00

abstract：:The intrinsic fluorescence of potato tuber pyrophosphate:fructose-6-phosphate 1-phosphotransferase (PFP) was used as an indicator of conformational changes due to ligand binding. Binding of the substrates and the allosteric activator fructose-2,6-bisphosphate was quantitatively compared to their respective kinetic eff...

journal_title：Archives of biochemistry and biophysics

authors： Podestá FE,Plaxton WC

更新日期：2003-06-01 00:00:00

abstract：:Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily and contribute to cell-cell adhesion and signal modulation in various tissues. In humans, several CEACAMs are targeted by pathogenic bacteria. One peculiar member of this family, CEACAM3, is exclusively expresse...

journal_title：Archives of biochemistry and biophysics

authors： Buntru A,Roth A,Nyffenegger-Jann NJ,Hauck CR

更新日期：2012-08-01 00:00:00

abstract：:Isolated mitochondria loaded with Ca(2+) and then exposed to Fe(2+) show a transient release of Ca(2+). The magnitude of this response depends on the Ca(2+) loading and the kinetics of the response depends on the concentration of added Fe(2+). We investigated the Fe(2+)-induced Ca(2+) release mechanism by measuring mi...

journal_title：Archives of biochemistry and biophysics

authors： Gogvadze V,Walter PB,Ames BN

更新日期：2002-02-15 00:00:00

abstract：:Biotin synthase catalyzes the insertion of a sulfur atom between two carbon atoms of dethiobiotin to form biotin in the last step of the biotin biosynthesis pathway. In Escherichia coli, biotin synthase is coded for by bioB gene. We report here cloning, sequencing, and initial functional characterization of the yeast ...

journal_title：Archives of biochemistry and biophysics

authors： Zhang S,Sanyal I,Bulboaca GH,Rich A,Flint DH

更新日期：1994-02-15 00:00:00

abstract：:Based on previous findings of increased nitric oxide synthase (NOS) expression in human gliomas (4), we hypothesized that peroxynitrite, a highly reactive metabolite of nitric oxide (NO) and superoxide (O(*-)(2)), might be increased in these tumors in vivo. Here we demonstrate that nitrotyrosine (a footprint of peroxy...

journal_title：Archives of biochemistry and biophysics

authors： Cobbs CS,Samanta M,Harkins LE,Gillespie GY,Merrick BA,MacMillan-Crow LA

更新日期：2001-10-15 00:00:00

abstract：:The kinetics of the irreversible unfolding of glutathione reductase (NAD[P]H:GSSG oxidoreductase, EC 1.6.4.2.) from cyanobacterium Spirulina maxima was studied at pH 7.0 and room temperature. Denaturation was induced by guanidinium chloride and the changes in enzyme activity, aggregation state, and tertiary structure ...

journal_title：Archives of biochemistry and biophysics

authors： Rendón JL,Mendoza-Hernández G

更新日期：2001-03-15 00:00:00

abstract：:Tripeptidyl-peptidase II (TPP II) is a cytosolic peptidase that has been implicated in fat formation and cancer, apparently independent of the enzymatic activity. In search for alternative functional regions, conserved motifs were identified and eleven signatures were constructed. Seven of the signatures covered previ...

journal_title：Archives of biochemistry and biophysics

authors： Eriksson S,Gutiérrez OA,Bjerling P,Tomkinson B

更新日期：2009-04-01 00:00:00

abstract：:The role of malnutrition in the development of cachexia in rats bearing the Yoshida ascites hepatoma AH-130 was investigated by comparing the changes in tissue protein turnover in these animals with those observed in pair-fed controls. The tumor elicited in rats an early and conspicuous loss of body weight and tissue ...

journal_title：Archives of biochemistry and biophysics

authors： Tessitore L,Costelli P,Bonetti G,Baccino FM

更新日期：1993-10-01 00:00:00

abstract：:The reduction of duroquinone (DQ), 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone (DB), and dichlorophenol indophenol (DCIP) by succinate and NADH was investigated in yeast mitochondria which have no spectrally detectable cytochrome b. Succinate reduces DB in the cytochrome b-deficient mitochondria at rates comparabl...

journal_title：Archives of biochemistry and biophysics

authors： Zhu QS,Sprague SG,Beattie DS

更新日期：1988-09-01 00:00:00

abstract：:A novel series of nonpeptidic compounds structurally related to the known anticholesteremic thyropropic acid were found to inhibit Escherichia coli peptide deformylase (PDF), with IC50 values in the low-micromolar range. Kinetic analysis of [4-(4-hydroxyphenoxy)-3,5-diiodophenyl]acetic acid reveals competitive inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Jayasekera MM,Kendall A,Shammas R,Dermyer M,Tomala M,Shapiro MA,Holler TP

更新日期：2000-09-15 00:00:00

abstract：:The topographical localization of the N-terminus of cytochrome b in the inner mitochondrial membrane was determined by mild proteolysis of the yeast mitochondrial cytochrome bc1 complex and identification of the proteolytic fragments derived from subunits of the complex with an established orientation in the inner mem...

journal_title：Archives of biochemistry and biophysics

authors： Beattie DS,Jenkins HC,Howton MM

更新日期：1994-07-01 00:00:00

abstract：:A radioimmunoassay was developed to assess the response of testicular HO-1 to agents known to increase the microsomal heme oxygenase activity. Treatment of rats with human chorionic gonadotropin (hCG) increased the microsomal heme oxygenase activity in rat testis. The following data suggest that the increase was speci...

journal_title：Archives of biochemistry and biophysics

authors： Kutty RK,Maines MD

更新日期：1989-01-01 00:00:00

abstract：:The nuclear Ah receptor from mouse hepatoma (Hepa-1c1c9) cells is a 176-kDa multimeric protein which is stable under conditions of up to 1 M KCl. Under denaturing conditions, the Hepa-1 nuclear receptor can be dissociated into a ligand-binding subunit of Mr approximately 91,000. The identity of subunits that compose t...

journal_title：Archives of biochemistry and biophysics

authors： Prokipcak RD,Denison MS,Okey AB

更新日期：1990-12-01 00:00:00

abstract：:A peptide containing 43 amino acid residues, rich in glutamic acid and lysine, was originally isolated from calf thymus and designated thymosin beta 4 [T.L.K. Low, S. -K. Hu, and A. L. Goldstein (1981) Proc. Nat. Acad. Sci. USA 78, 1162-1166]. However, thymosin beta 4 was also shown to be present in other tissues of r...

journal_title：Archives of biochemistry and biophysics

authors： Erickson-Viitanen S,Ruggieri S,Natalini P,Horecker BL

更新日期：1983-03-01 00:00:00

abstract：:Myeloperoxidase (MPO) is a heme-containing peroxidase expressed mainly in neutrophils and to a lesser degree in monocytes. In the presence of hydrogen peroxide and halides, MPO catalyzes the formation of reactive oxygen intermediates, including hypochlorous acid (HOCl). The MPO/HOCl system plays an important role in m...

journal_title：Archives of biochemistry and biophysics

authors： Aratani Y

更新日期：2018-02-15 00:00:00

abstract：:Rodobacter capsulatus cells, which were cultured anaerobically in high light intensity, had fewer foldings in the cytoplasmic membrane than those which were grown in lower light intensities. Spheroplast-derived membrane fractions obtained from cells cultured under high light intensity contained a high yield of large r...

journal_title：Archives of biochemistry and biophysics

authors： Carmeli C,Lifshitz Y,Friedberg I

更新日期：1991-08-01 00:00:00

abstract：:The relationship between ligand-protein affinity and the extent of protein stabilization induced by such interactions has been investigated using the binding of polyanions to acidic fibroblast growth factor (aFGF) as a model system. It was found that the experimentally observed unfolding rate constant of aFGF consists...

journal_title：Archives of biochemistry and biophysics

authors： Mach H,Middaugh CR

更新日期：1994-02-15 00:00:00

abstract：:Carbohydrate Binding Modules (CBMs) targeting cellulose, xylan and mannan have been reported, however, a CBM targeting rhamnogalacturonan I (RG I) has never been identified. We had studied earlier a rhamnogalacturonan lyase (CtRGL) from Clostridium thermocellum that was associated with a family 35 CBM, Rgl-CBM35. In t...

journal_title：Archives of biochemistry and biophysics

authors： Dhillon A,Sharma K,Rajulapati V,Goyal A

更新日期：2018-09-15 00:00:00

abstract：:The native conformation of bovine retinal arrestin has been characterized by a variety of spectroscopic methods. The purified protein gives rise to a near uv absorption band centered at 279 nm which results from the absorbance of its 14 tyrosine and one tryptophan residue. The extinction coefficient for this absorptio...

journal_title：Archives of biochemistry and biophysics

authors： Kotake S,Hey P,Mirmira RG,Copeland RA

更新日期：1991-02-15 00:00:00

abstract：:Prostacyclin (PGI(2)) synthase is a heme-thiolate (P450) protein which reacts with low levels of peroxynitrite (PN) under tyrosine nitration and inactivation. Studying heme proteins as models, we have found the heme-thiolate protein NADH-NO reductase (P450(NOR)) to be highly efficient in decomposing PN under concomita...

journal_title：Archives of biochemistry and biophysics

authors： Zou MH,Daiber A,Peterson JA,Shoun H,Ullrich V

更新日期：2000-04-01 00:00:00