Rat liver glucose-6-phosphatase system: light scattering and chemical characterization.

Abstract:

:Glucose-6-phosphatase is a multicomponent system located in the endoplasmic reticulum, involving both a catalytic subunit (G6PC) and several substrate and product carriers. The glucose-6-phosphate carrier is called G6PT1. Using light scattering, we determined K(D) values for phosphate and glucose transport in rat liver microsomes (45 and 33mM, respectively), G6PT1 K(D) being too low to be estimated by this technique. We provide evidence that phosphate transport may be carried out by an allosteric multisubunit translocase or by two distinct proteins. Using chemical modifications by sulfhydryl reagents with different solubility properties, we conclude that in G6PT1, one thiol group important for activity is facing the cytosol and could be Cys(121) or Cys(362). Moreover, a different glucose-6-phosphate translocase, representing 20% of total glucose-6-phosphate transport and insensitive to N-ethylmaleimide modification, could coexist with liver G6PT1. In the G6PC protein, an accessible thiol group is facing the cytosol and, according to structural predictions, could be Cys(284).

journal_name

Arch Biochem Biophys

authors

Clottes E,Middleditch C,Burchell A

doi

10.1016/s0003-9861(02)00523-4

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

33-41

issue

1

eissn

0003-9861

issn

1096-0384

pii

S0003986102005234

journal_volume

408

pub_type

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