Carbon-13 isotope effects on reactions involving carbamate and carbamoyl phosphate.

abstract：:Methionine is oxidized to methionine sulfoxide by rat liver and kidney microsomes in an O2- and NADPH-dependent manner. In all microsomal assays, no methionine sulfone was detected. Use of a monoclonal antibody to rat liver cytochrome P-450 reductase, various cytochrome P-450 and peroxidase inhibitors, antioxidants, a...

journal_title：Archives of biochemistry and biophysics

authors： Krause RJ,Ripp SL,Sausen PJ,Overby LH,Philpot RM,Elfarra AA

更新日期：1996-09-01 00:00:00

abstract：:As a means of comparing the functional properties of an enzyme in dilute solution in vitro with those for the same enzyme acting in its normal cellular environment, a study was conducted with 4-substituted pyrazoles as inhibitors of rat liver alcohol dehydrogenase in vitro and ethanol oxidation in isolated rat hepatoc...

journal_title：Archives of biochemistry and biophysics

authors： Cornell NW,Hansch C,Kim KH,Henegar K

更新日期：1983-11-01 00:00:00

abstract：:Alkaline phosphatase (AP) was purified to over 90% homogeneity from rat osteosarcoma by acetone precipitation followed by chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxyapatite. The purified enzyme had a specific activity of 759 units/mg protein at its optimal pH (10.5), and a Km of 0.8 mM for p-nitroph...

journal_title：Archives of biochemistry and biophysics

authors： Nair BC,Majeska RJ,Rodan GA

更新日期：1987-04-01 00:00:00

abstract：:Oxygen free radicals and hydroperoxides have been postulated to play a causal role in the aging process, implying that antioxidant enzymes may act as longevity determinants. Catalase (H2O2:H2O2 oxidoreductase; EC1.11.1.6) is the sole enzyme involved in the elimination of H2O2 in Drosophila melanogaster; glutathione pe...

journal_title：Archives of biochemistry and biophysics

authors： Orr WC,Sohal RS

更新日期：1992-08-15 00:00:00

abstract：:Three strains of Escherichia coli differing only in the catalase locus mutated by transposon Tn10 were constructed. These strains produced only catalase HPI (katE::Tn10 and katF::Tn10 strains) or catalase HPII (katG::Tn10). HPI levels increased gradually about twofold during logarithmic growth but did not increase dur...

journal_title：Archives of biochemistry and biophysics

authors： Loewen PC,Switala J,Triggs-Raine BL

更新日期：1985-11-15 00:00:00

abstract：:Previous work from this laboratory indicated that the N-terminus of recombinant human cytochrome P450 (P450) 1A2 expressed in Escherichia coli is blocked (P. Sandhu, Z. Guo, T. Baba, M. V. Martin, R. H. Tukey, and F. P. Guengerich, (1994) Arch. Biochem. Biophys. 30, 168 -177). A modification of this construct was done...

journal_title：Archives of biochemistry and biophysics

authors： Dong MS,Yamazaki H,Guo Z,Guengerich FP

更新日期：1996-03-01 00:00:00

abstract：:Since experiments with freshly isolated rat hepatocytes have shown that cellular vitamin E is consumed in response to insult by compounds that induce an oxidative stress only after cellular glutathione (GSH) concentrations have been substantially depleted, experiments were performed to determine whether this sequence ...

journal_title：Archives of biochemistry and biophysics

authors： Warren DL,Reed DJ

更新日期：1991-02-15 00:00:00

abstract：:Enzymes subjected to freeze-thawing are known to be protected by polymers that are preferentially excluded from the hydrated surface of proteins [reviewed in Carpenter et al. (1994) ACS Symp. Ser. 567, 134-147]. Preferentially excluded solutes are also known to stabilize quaternary structure, which enhances the thermo...

journal_title：Archives of biochemistry and biophysics

authors： Anchordoquy TJ,Carpenter JF

更新日期：1996-08-15 00:00:00

abstract：:A novel halophilic, alkalithermostable lipase LipR2 from Alkalispirillum sp. NM-ROO2 was cloned and expressed. LipR2 was covalently immobilized on Florisil® functionalized with glutaraldehyde. Protein binding efficiency of functionalized Florisil® was 94.7%. Immobilized LipR2 retained 97.5% of specific activity of the...

journal_title：Archives of biochemistry and biophysics

authors： Mesbah NM

更新日期：2019-05-30 00:00:00

abstract：:Human plasma glutathione peroxidase was purified to homogeneity and partially sequenced. Overlapping peptide fragments from three endopeptidase digests permitted the determination of one sequence of 32 contiguous amino acids and one sequence of 23 contiguous amino acids. Five additional unique peptide sequences withou...

journal_title：Archives of biochemistry and biophysics

authors： Esworthy RS,Chu FF,Paxton RJ,Akman S,Doroshow JH

更新日期：1991-05-01 00:00:00

abstract：:Phosphatidylserine (PS) is predominantly confined to the inner leaflet of plasma membrane in cells, but it is externalized on the cell surface during apoptosis. This externalized PS is required for effective phagocytosis of apoptotic cells by macrophages. Because PS trans-bilayer asymmetry is not absolute in different...

journal_title：Archives of biochemistry and biophysics

authors： Borisenko GG,Matsura T,Liu SX,Tyurin VA,Jianfei J,Serinkan FB,Kagan VE

更新日期：2003-05-01 00:00:00

abstract：:The modes of action of a Vero toxin (VT2 or Shiga-like toxin II) from Escherichia coli, of ricin, and of alpha-sarcin were compared. Elongation factor 1 (EF1) and GTP-dependent Phe-tRNA binding to ribosomes in the presence of poly(U) was inhibited by these three toxins, but EF1 and guanylyl (beta, gamma-methylene)-dip...

journal_title：Archives of biochemistry and biophysics

authors： Furutani M,Kashiwagi K,Ito K,Endo Y,Igarashi K

更新日期：1992-02-14 00:00:00

abstract：:The radioactive, photoactivatable labeling probe [beta-32P]5-azidouridine 5'-diphosphoglucose has recently been shown to label a 62-kDa protein in crude homogenates and in partially purified enzyme preparations without photoactivation. Here, we report that a portion of this radioactivity is due to labeling of phosphog...

journal_title：Archives of biochemistry and biophysics

authors： Marchase RB,Richardson KL,Srisomsap C,Drake RR,Haley BE

更新日期：1990-07-01 00:00:00

abstract：:Cutaneous malignant melanomas originate primarily within epidermal melanocytic cells. Melanoma cells share many characteristics with melanocyte precursors, suggesting that melanoma cells utilize the developmental programs of their normal counterpart for their own progression. The pigmentation system provides an advant...

journal_title：Archives of biochemistry and biophysics

authors： Liu J,Fukunaga-Kalabis M,Li L,Herlyn M

更新日期：2014-12-01 00:00:00

abstract：:The effects of pH, NaCl, and malate2- on the equilibrium between dimeric and higher-molecular-weight forms of NAD malic enzyme from Solanum tuberosum var. Chieftain have been analyzed by monitoring the kinetic changes associated with disaggregation [S. D. Grover and R. T. Wedding (1982) Plant Physiol. 70, 1169-1172]. ...

journal_title：Archives of biochemistry and biophysics

authors： Grover SD,Wedding RT

更新日期：1984-11-01 00:00:00

abstract：:Tritium-labeled 24(R)-hydroxyvitamin D3 and 24(S)-hydroxyvitamin D3 were chemically synthesized and the 1 alpha-hydroxylation of these compounds by chick kidney homogenates was studied. A marked stereospecific preference with regard to the orientation of the hydroxyl functionality on carbon-24 was noted: while the 24(...

journal_title：Archives of biochemistry and biophysics

authors： Ishizuka S,Bannai K,Norman AW

更新日期：1983-09-01 00:00:00

abstract：:Tyr-503 of beta-galactosidase was specifically replaced with Phe, His, Cys, and Lys using site-directed mutagenesis. The normal enzyme and the substituted enzymes were purified. The activities of each of the substituted enzymes with o-nitrophenyl-beta-D-galactopyranoside (ONPG) and p-nitrophenyl-beta-D-galactopyronosi...

journal_title：Archives of biochemistry and biophysics

authors： Ring M,Huber RE

更新日期：1990-12-01 00:00:00

abstract：:The hexapeptide Hyp-Gly-Pro-Lys-Gly-Glu was synthesized as a potential substrate for collagen lysyl hydroxylase. Kinetic data on the interaction of this peptide with purified chicken embryo lysyl hydroxylase showed that the hexapeptide is a moderately good substrate having Km, Vmax, and Kcat/Km values comparable to th...

journal_title：Archives of biochemistry and biophysics

authors： Ananthanarayanan VS,Saint-Jean A,Jiang P

更新日期：1992-10-01 00:00:00

abstract：:Tuberculosis remains a leading cause of human mortality. The emergence of strains of Mycobacterium tuberculosis, the causative agent, that are resistant to the major frontline antitubercular drugs increases the urgency for the development of new therapeutic agents. Sequencing of the M. tuberculosis genome revealed the...

journal_title：Archives of biochemistry and biophysics

authors： Ouellet H,Johnston JB,Ortiz de Montellano PR

更新日期：2010-01-01 00:00:00

abstract：:DLIF (digoxin-like immunoreactive factor) and OLF (ouabain-like factor) are endogenous steroid-like ligands (approximately 781 and 595 Da, respectively) with molecular and structural properties similar to the plant-derived cardiac glycosides, digoxin and ouabain. We developed a purification method with a sufficiently ...

journal_title：Archives of biochemistry and biophysics

authors： Qazzaz HM,Valdes R Jr

更新日期：1996-04-01 00:00:00

abstract：:Low-molecular-weight (LMW) thiols are a class of highly reactive compounds massively involved in the maintenance of cellular redox homeostasis. They are implicated in plant responses to almost all stress factors, as well as in the regulation of cellular metabolism. The most studied LMW thiols are glutathione and its b...

journal_title：Archives of biochemistry and biophysics

authors： Pivato M,Fabrega-Prats M,Masi A

更新日期：2014-10-15 00:00:00

abstract：:The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is a basic helix-loop-helix transcription factor. The hepatic expression of SHARP-2 mRNA was investigated under various conditions. The level was decreased in the regenerating rat liver and malignant hepatoma cells. In contrast, the expression of SHARP-2...

journal_title：Archives of biochemistry and biophysics

authors： Hirano S,Yamada K,Kawata H,Shou Z,Mizutani T,Shigematsu Y,Mayumi M,Miyamoto K

更新日期：2004-02-01 00:00:00

abstract：:Human seminal fluid was recently found to contain 18,19-dehydroprostaglandins E1 and E2 (E. H. Oliw, H. Sprecher, and M. Hamberg, (1986) J. Biol. Chem. 261, 2675-2683). In the present study, the cis and trans isomers of 18,19-dehydroprostaglandins E1 and E2 were prepared by incubation of microsomes of ram vesicular gl...

journal_title：Archives of biochemistry and biophysics

authors： Oliw EH,Benthin G,Hamberg M

更新日期：1987-10-01 00:00:00

abstract：:Measurement of the macular pigment optical density (MPOD) by heterochromatic flicker photometry (HFP) is accomplished by viewing a small circular stimulus that alternates between a test wavelength that is absorbed by the MP (typically--blue, 460 nm) and a reference wavelength that is not absorbed (typically-green, 540...

journal_title：Archives of biochemistry and biophysics

authors： Bone RA,Landrum JT

更新日期：2004-10-15 00:00:00

abstract：:Complete primary structures of five allergenically active oligosaccharitols (HPG-beta 2-N5a, -N6, -N7a, -N7b, and -N9) derived from a sea squirt H-antigen were studied. Structural characterization was carried out by a new method in which products of limited periodate oxidation, followed by derivatization with p-aminob...

journal_title：Archives of biochemistry and biophysics

authors： Ohta M,Matsuura F,Kobayashi Y,Shigeta S,Ono K,Oka S

更新日期：1991-11-01 00:00:00

abstract：:Endothelial senescence is the main risk factor that contributes to vascular dysfunction and the progression of vascular disease. Carbon monoxide (CO) plays an important role in preventing vascular dysfunction and in maintaining vascular physiology or homeostasis. The application of exogenous CO has been shown to confe...

journal_title：Archives of biochemistry and biophysics

authors： Zheng M,Chen Y,Park J,Song HC,Chen Y,Park JW,Joe Y,Chung HT

更新日期：2019-11-30 00:00:00

abstract：:The enzyme catalase catalyzes the breakdown of hydrogen peroxide into oxygen and water. It is the main regulator of hydrogen peroxide metabolism. Hydrogen peroxide is a highly reactive small molecule formed as a natural byproducts of energy metabolism. Excessive concentrations may cause significant damages to protein,...

journal_title：Archives of biochemistry and biophysics

authors： Góth L,Nagy T

更新日期：2012-09-15 00:00:00

abstract：:Electron spin lattice relaxation times (T1) and the phase memory times (Tm) were obtained for the synthetic melanin system from 3-hydroxytyrosine (dopa) by means of electron spin echo spectroscopy at 77 degrees K. Saturation behavior of the ESR spectra of melanins in melanin-containing tissue and of the synthetic mela...

journal_title：Archives of biochemistry and biophysics

authors： Okazaki M,Kuwata K,Miki Y,Shiga S,Shiga T

更新日期：1985-10-01 00:00:00

abstract：:The thermodynamics of the drug-inhibitors acyclovir, ganciclovir, and 9-benzylguanine binding to human purine nucleoside phosphorylase (hsPNP) were determined from isothermal titration calorimetry as a function of the substrate phosphate ion (Pi) concentration from 0 to 0.125 M and temperature from 15 degrees C to 35 ...

journal_title：Archives of biochemistry and biophysics

authors： Todorova NA,Schwarz FP

更新日期：2008-12-15 00:00:00

abstract：:The oxidation of 6-hydroxy-2,2,5,7,8-pentamethylchroman, Trolox C, and alpha-tocopherol by horseradish peroxidase was examined by stopped-flow and ESR experiments. The catalytic intermediate of horseradish peroxidase during the oxidation of vitamin E analogues and vitamin E was invariably Compound II, and rate constan...

journal_title：Archives of biochemistry and biophysics

authors： Nakamura M,Hayashi T

更新日期：1992-12-01 00:00:00