A residue-level investigation of the equilibrium unfolding of the C2A domain of synaptotagmin 1.

abstract：:Erythropoietin was purified to homogeneity from the culture medium of a baby hamster kidney cell line stably transfected with a human erythropoietin gene. A three-step procedure was used, which included affinity chromatography, ion-exchange chromatography, and reverse-phase chromatography. Purity of the protein was co...

journal_title：Archives of biochemistry and biophysics

authors： Broudy VC,Tait JF,Powell JS

更新日期：1988-09-01 00:00:00

abstract：:hCD157 catalyzes the hydrolysis of nicotinamide riboside (NR) and nicotinic acid riboside (NAR). The release of nicotinamide or nicotinic acid from NR or NAR was confirmed by spectrophotometric, HPLC and NMR analyses. hCD157 is inactivated by a mechanism-based inhibitor, 2'-deoxy-2'-fluoro-nicotinamide arabinoside (fN...

journal_title：Archives of biochemistry and biophysics

authors： Preugschat F,Carter LH,Boros EE,Porter DJ,Stewart EL,Shewchuk LM

更新日期：2014-12-15 00:00:00

abstract：:A cytoplasmic 10S ribonucleoprotein particle (iRNP), which is isolated from chick embryonic muscle, is a potent inhibitor of mRNA translation in vitro and contains a 4S translation inhibitory RNA species (iRNA). The iRNP particle shows similarity in size to the small nuclear ribonucleoprotein (snRNP) particles. Certai...

journal_title：Archives of biochemistry and biophysics

authors： Boak AM,Kovacs SA,Agris PF,Chakraborty D,Sarkar S

更新日期：1986-07-01 00:00:00

abstract：:The regulation of phosphofructokinase during development of C2C12 myoblasts to myotubes was investigated. Enzyme activity was markedly increased during myogenic development. The increase was observed when enzyme activity was measured under optimal conditions and was not due to changes in the allosteric kinetic propert...

journal_title：Archives of biochemistry and biophysics

authors： Wills KN,Mansour TE

更新日期：1990-04-01 00:00:00

abstract：:Asthma is characterized by a predominant T(H)2 type immune response to airborne allergens. Controlling T(H)2 cell function has been proposed as therapy for this disease. We show here that ligands for the nuclear receptor peroxisome proliferator activated receptor (PPAR)gamma significantly reduced the immunological sym...

journal_title：Archives of biochemistry and biophysics

authors： Mueller C,Weaver V,Vanden Heuvel JP,August A,Cantorna MT

更新日期：2003-10-15 00:00:00

abstract：:A detailed knowledge about the substrate specificities of the heparin lyases is necessary when using these enzymes as tools for elucidating the sequence of heparin and heparan sulfate. The substrate specificity of heparin lyases I, II, and III have been profiled with structurally defined, heparin-derived oligosacchari...

journal_title：Archives of biochemistry and biophysics

authors： Desai UR,Wang HM,Linhardt RJ

更新日期：1993-11-01 00:00:00

abstract：:The influence of transmembrane ion fluxes on mycoplasma membrane potentials was studied. Electric membrane potential was calibrated vs fluorescence intensity of a potential-sensitive carbocyanine dye according to delta psi = (RT/F) X log([aIN(1 - IN) - b]/Kint), where IN = I/I0, I0 = maximal fluorescence intensity (ob...

journal_title：Archives of biochemistry and biophysics

authors： Schummer U,Schiefer HG

更新日期：1986-02-01 00:00:00

abstract：:Versiconal cyclase catalyzes the dehydration of versiconal to versicolorin B or versicolorin C [versicolorin B(C)]. The enzyme was purified from mycelia of Aspergillus parasiticus by DEAE-cellulose, hydroxylapatite, and Mono Q column chromatography. The protein contains two identical subunits of molecular weight 72,00...

journal_title：Archives of biochemistry and biophysics

authors： Lin BK,Anderson JA

更新日期：1992-02-14 00:00:00

abstract：:It has been proposed that negatively charged amino acids on the surface of reductase and positively charged amino acids on the surface of P450 mediate the binding of both proteins through electrostatic interactions. In this study, we used a site-directed mutagenesis approach to determine a role for two lysine residues...

journal_title：Archives of biochemistry and biophysics

authors： Cvrk T,Strobel HW

更新日期：2001-01-15 00:00:00

abstract：:Cytochrome P450 reductase was purified to homogeneity from cell suspension cultures of the opium poppy Papaver somniferum, the enzyme was characterized (K(m) cytochrome c, 8.3 microM; K(m) NADPH, 4.2 microM; pH optimum, 8.0; M(r), 80 kDa), and the amino acid sequence of internal peptides was determined. Partial cDNA c...

journal_title：Archives of biochemistry and biophysics

authors： Rosco A,Pauli HH,Priesner W,Kutchan TM

更新日期：1997-12-15 00:00:00

abstract：:We examined effects of three structurally related pyridinium compounds, 1-methyl-4-phenylpyridinium (MPP+), paraquat, and 1-methyl-4-(4'-nitrophenyl) pyridinium (analog 1), on the energy metabolism in pheochromocytoma PC12 cells. MPP+ inhibited the intracellular NADH oxidation by the mitochondrial respiratory chain, j...

journal_title：Archives of biochemistry and biophysics

authors： Kang D,Miyako K,Kuribayashi F,Hasegawa E,Mitsumoto A,Nagano T,Takeshige K

更新日期：1997-01-01 00:00:00

abstract：:Long-term high-calcium intake and intestinal calcium hyperabsorption are hazardous to the body. It is hypothesized that enterocytes possess mechanisms for preventing superfluous calcium absorption, including secretion of negative regulators of calcium absorption and utilization of calcium-sensing receptor (CaSR) to de...

journal_title：Archives of biochemistry and biophysics

authors： Wongdee K,Rodrat M,Keadsai C,Jantarajit W,Teerapornpuntakit J,Thongbunchoo J,Charoenphandhu N

更新日期：2018-11-01 00:00:00

abstract：:The unicellular marine phytoplankton Chattonella marina is known to have toxic effects against various living marine organisms, especially fishes. However, details of the mechanism of the toxicity of this plankton remain obscure. Here we demonstrate the generation of superoxide and hydroxyl radicals from a red tide un...

journal_title：Archives of biochemistry and biophysics

authors： Oda T,Akaike T,Sato K,Ishimatsu A,Takeshita S,Muramatsu T,Maeda H

更新日期：1992-04-01 00:00:00

abstract：:Coupling of the narrow 311-nm Gd3+ fluorescence transition with the vibrational transitions of its inner-sphere ligands gives rise to the emission of vibronic photons between 320 and 350 nm. The energy difference between the fluorescence and vibronic transitions is equal to that of the vibrational transitions of the m...

journal_title：Archives of biochemistry and biophysics

authors： MacGregor RB Jr

更新日期：1989-10-01 00:00:00

abstract：:Mucus hypersecretion is a common feature in chronic airway diseases, and serine proteases play a critical role in this process. However, the mechanisms by which serine proteases induce mucin5AC hypersecretion have not been fully explored. In this study, we characterized human airway trypsin-like protease (HAT), a seri...

journal_title：Archives of biochemistry and biophysics

authors： Liu C,Li Q,Zhou X,Kolosov VP,Perelman JM

更新日期：2013-07-15 00:00:00

abstract：:By anion-exchange chromatography, the nitrogen fixing photosynthetic stem nodules and nonphotosynthetic root nodules of Sesbania rostrata are shown to contain the same seven major components of leghemoglobin (Lb), numbered LbI-LbVII in order of elution, although in different proportions. No novel component was found i...

journal_title：Archives of biochemistry and biophysics

authors： Bogusz D,Kortt AA,Appleby CA

更新日期：1987-04-01 00:00:00

abstract：:Earlier studies have shown that the Fur (ferric uptake regulation) protein acts as an anaerobic/aerobic repressor of MnSOD (sodA) expression. We found that the aerobic expression of sodA::lacZ fusion in a Fur- background to be threefold higher than in a Fur+ background. This effect of fur mutation was not seen in a st...

journal_title：Archives of biochemistry and biophysics

authors： Schrum LW,Hassan HM

更新日期：1994-03-01 00:00:00

abstract：:Using 31P NMR spectroscopy, we have measured the rate of ATP synthesis, and the free concentrations of ATP, ADP, cytoplasmic Pi, and H+ in maize root tips under a wide range of conditions. We show that the ratio [ATP]/[ADP] in normoxic root tips is greater than 25. We found no simple relationship between the concentra...

journal_title：Archives of biochemistry and biophysics

authors： Roberts JK,Lane AN,Clark RA,Nieman RH

更新日期：1985-08-01 00:00:00

abstract：:Previously, we reported that emptying of intracellular Ca(2+) pools with endoplasmatic Ca(2+)-ATP-ase inhibitor thapsigargin leads to the Na(+) influx in human lymphocytes (M. Tepel et al., 1994, J. Biol. Chem. 269, 26239-26242). In the present study we examined the mechanism underlying the thapsigargin-induced Na(+) ...

journal_title：Archives of biochemistry and biophysics

authors： Nofer JR,Junker R,Walter M,Seedorf U,Assmann G,Zidek W,Tepel M

更新日期：2000-02-15 00:00:00

abstract：:The intracellular location of pyruvate carboxylase (EC 6.4.1.1) in rat liver and Saccharomyces cerevisiae was investigated using the antibody-gold and protein A-gold techniques carried out as a postembedding immunoelectron microscopic procedure. The vast majority of gold particles (greater than 98%), indicative of the...

journal_title：Archives of biochemistry and biophysics

authors： Rohde M,Lim F,Wallace JC

更新日期：1991-10-01 00:00:00

abstract：:Appropriate dietary interventions may reduce the potentially damaging effects of free radicals generated during metabolism and various physiological conditions. We have investigated the effects of dietary vitamins C, E, beta-carotene, or selenium (Se) on the activity of endogenous antioxidant enzymes and respiratory c...

journal_title：Archives of biochemistry and biophysics

authors： Desai VG,Casciano D,Feuers RJ,Aidoo A

更新日期：2001-10-15 00:00:00

abstract：:Heparin infusion in an experimental animal model of arterial injury causes a significant increase in the proteoglycan (PG) component of the extracellular matrix in the injured arteries (Snow, A. D., Bolender, R. P., Wight, T. N., and Clowes, A. W. (1990) Am. J. Pathol. 137, 313-330). The mechanisms responsible for thi...

journal_title：Archives of biochemistry and biophysics

authors： Potter-Perigo S,Wight TN

更新日期：1996-12-01 00:00:00

abstract：:Hitherto the lack of success in cloning the aryl hydrocarbon (Ah) receptor has severely hindered its analysis. The recent cloning of cDNAs for two subunits of the receptor should lead to a renaissance in research. Furthermore, the sequences of the two subunits, as deduced from their cDNA sequences, have shown that the...

journal_title：Archives of biochemistry and biophysics

authors： Hankinson O

更新日期：1993-01-01 00:00:00

abstract：:Fibronectin heterogeneity is, in part, the result of post-translational modifications. In these experiments, cartilage fibronectins were purified by anion exchange chromatography, followed by gelatin affinity chromatography or immunoprecipitation, and, finally, sodium dodecyl sulfate--polyacrylamide gel electrophoresi...

journal_title：Archives of biochemistry and biophysics

authors： Burton-Wurster N,Lust G

更新日期：1993-11-01 00:00:00

abstract：:The fertilization layer of Xenopus laevis eggs is formed by the cortical granule lectin binding to its ligand. The binding requires Ca2+, is specific for galactose, and functionally establishes a block to polyspermy at fertilization. We have designed a new enzyme-linked lectin assay for the cortical granule lectin (CG...

journal_title：Archives of biochemistry and biophysics

authors： Quill TA,Hedrick JL

更新日期：1996-09-15 00:00:00

abstract：:Cytosolic extracts from the posterior adductor muscle of the bivalve mollusk Mytilus galloprovincialis contain significant amounts of both cGMP-binding and cGMP-stimulated protein kinase activities. However, photoaffinity labeling with 8-azido-[32P]cGMP revealed only a major cGMP-binding protein with an apparent molec...

journal_title：Archives of biochemistry and biophysics

authors： Rodríguez JL,Barcia R,Ramos-Martínez JI,Villamarín JA

更新日期：1998-11-01 00:00:00

abstract：:This paper describes a theory for the mechanism of three-state transition of proteins which is often observed in aqueous organic cosolvent systems, i.e., from the native, via intermediate to helical forms. The first transition, accompanied by changes in the tertiary and/or secondary structures, was explained by larger...

journal_title：Archives of biochemistry and biophysics

authors： Arakawa T,Goddette D

更新日期：1985-07-01 00:00:00

abstract：:Antioxidative flavonoids, ubiquitously included in vegetables, fruits and teas, are expected to prevent degenerative diseases. It is unclear, however, whether flavonoids can enter the cellular nuclei and suppress the oxidative damage of DNA. Here, several flavonoids at the physiological concentration of 10 microM were...

journal_title：Archives of biochemistry and biophysics

authors： Kanazawa K,Uehara M,Yanagitani H,Hashimoto T

更新日期：2006-11-15 00:00:00

abstract：:Three strains of Escherichia coli differing only in the catalase locus mutated by transposon Tn10 were constructed. These strains produced only catalase HPI (katE::Tn10 and katF::Tn10 strains) or catalase HPII (katG::Tn10). HPI levels increased gradually about twofold during logarithmic growth but did not increase dur...

journal_title：Archives of biochemistry and biophysics

authors： Loewen PC,Switala J,Triggs-Raine BL

更新日期：1985-11-15 00:00:00

abstract：:Bovine liver glutamate dehydrogenase reacts covalently with 2-(4-bromo-2,3-dioxobutylthio)adenosine 5'-monophosphate (2-BDB-TAMP) with incorporation of 1 mol reagent/mol enzyme subunit and loss of one of the two ADP sites of native enzyme [S. P. Batra and R. F. Colman, J. Biol. Chem. 261, 15565-15571 (1986)]. Incorpor...

journal_title：Archives of biochemistry and biophysics

authors： Batra SP,Lark RH,Colman RF

更新日期：1989-04-01 00:00:00