当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Cytochromes P450 2C1/2 and P450 2E1 are retained in the endoplasmic reticulum membrane by different mechanisms.

Cytochromes P450 2C1/2 and P450 2E1 are retained in the endoplasmic reticulum membrane by different mechanisms.

Abstract：

:Cytochrome P450 (P450) 2C1/2 contains redundant endoplasmic reticulum (ER) retention signals and is excluded from the recycling pathway. Other P450s, such as P450 2E1, have been detected in the plasma membrane and Golgi apparatus. To examine whether the mechanisms of ER retention might differ for P450 2C1/2 and P450 2E1, chimeras of green flourescent protein and the full-length proteins, N-terminal signal/anchor sequences, or the cytoplasmic catalytic domains from these proteins have been expressed in COS1 cells. Chimeras with either the N-terminal signal/anchor sequence or the cytoplasmic domain of P450 2C1/2 were retained in the ER and the distribution was not altered by treatment with nocodazole. A chimera with full-length P450 2E1 was located in the ER, but in contrast to P450 2C1/2, treatment with nocodazole resulted in redistribution to a vesicular pattern, which suggested that this protein was retained in the ER by a retrieval mechanism. In support of this possibility, the P450 2E1 chimera, but not the P450 2C1/2 chimera, was included in transport vesicles generated in an in vitro budding assay. A chimera with only the N-terminal signal/anchor sequence of P450 2E1 fused to green fluorescent protein was located in the ER and nocodazole treatment altered its distribution, whereas a chimera with only the cytoplasmic domain of P450 2E1 was not efficiently retained in the ER and accumulated primarily in the Golgi region. These results demonstrate that the mechanisms for retention in the ER of two closely related members of the P450 superfamily are different and that the N-terminal signal/anchor sequence contains the dominant retention signal.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Szczesna-Skorupa E,Chen CD,Kemper B

doi

10.1006/abbi.1999.1628

subject

Has Abstract

pub_date

2000-02-15 00:00:00

pages

128-36

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(99)91628-4

journal_volume

374

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Induction of collagenase mRNA in lapine articular chondrocytes by synovial factors and interleukin-1.

    abstract：:The cDNA probe H-9, originally constructed to recognize a portion of the mRNA for lapine synovial collagenase, also hybridized with a RNA of the same size (approximately 2.0 kb) isolated from activated lapine articular chondrocytes. Primary, monolayer cultures of lapine articular chondrocytes did not contain detectabl...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90605-4

    authors： Lin CW,Phillips SL,Brinckerhoff CE,Georgescu HI,Bandara G,Evans CH

    更新日期：1988-07-01 00:00:00

  • Bovine liver dihydropyrimidine amidohydrolase: pH dependencies of the steady-state kinetic and proton relaxation rate properties of the Mn(II)-containing enzyme.

    abstract：:The essential Zn(II) in bovine liver dihydropyrimidine amidohydrolase (DHPase) was removed by incubation with 2,6-dipicolinic acid and replaced with Mn(II). Electron paramagnetic resonance studies of Mn(II) binding show that there are four binding sites per tetramer, and the dissociation constant at pH 7.5 is 13.5 mic...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90526-1

    authors： Lee MH,Pettigrew DW,Sander EG,Nowak T

    更新日期：1987-12-01 00:00:00

  • In vivo regulation of phosphorylation level and activity of phosphofructokinase by serotonin in Fasciola hepatica.

    abstract：:The level of phosphorylation and activation of phosphofructokinase by serotonin (5-hydroxytryptamine) was studied in intact liver flukes Fasciola hepatica. The enzyme was immunoprecipitated with antiserum prepared against pure enzyme from the liver flukes. The resuspended immunoprecipitated enzyme retained most of its...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90307-x

    authors： Kamemoto ES,Lan L,Mansour TE

    更新日期：1989-06-01 00:00:00

  • Differential effects of heparin and glucose on structural conformation of human alpha1 antitrypsin: evidence for a heparin-induced cleaved form of the inhibitor.

    abstract：:alpha1 Antitrypsin (alpha1AT) is the archetypal member of the serpin superfamily. Current knowledge of its inhibitory mechanism does not provide for any heparin-induced enhancement of serine proteinase inhibition. Since previous results have shown that an apparently altered alpha1AT form may be purified from the plasm...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1997.0317

    authors： Finotti P,de Laureto PP

    更新日期：1997-11-01 00:00:00

  • Catalytic properties of the inorganic pyrophosphatase in rat liver mitochondria.

    abstract：:Intact rat liver mitochondria have very low hydrolytic activity, if any, toward exogenous pyrophosphate. The activity can be unmasked by making mitochondria permeable to PPi by toluene treatment or disrupting them with detergents or ultrasound, indicating that the active site of pyrophosphatase is located in the matri...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90044-w

    authors： Dubnova EB,Baykov AA

    更新日期：1992-01-01 00:00:00

  • Evidence for an inhibitory LIM domain in a rat brain agmatinase-like protein.

    abstract：:We recently cloned a rat brain agmatinase-like protein (ALP) whose amino acid sequence greatly differs from other agmatinases and exhibits a LIM-like domain close to its carboxyl terminus. The protein was immunohistochemically detected in the hypothalamic region and hippocampal astrocytes and neurons. We now show that...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2011.05.003

    authors： Castro V,Fuentealba P,Henríquez A,Vallejos A,Benítez J,Lobos M,Díaz B,Carvajal N,Uribe E

    更新日期：2011-08-01 00:00:00

  • Interaction of bovine glutamate dehydrogenase with isoquinoline alkaloids.

    abstract：:Representatives of four different classes of isoquinoline alkaloids (aporphines, benzylisoquinolines, tetrahydroisoquinolines, and phthalideisoquinolines) inhibit glutamate dehydrogenase activity in both reaction directions. Representatives of five other groups of isoquinoline alkaloids have been without any significa...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90144-3

    authors： Kovár J

    更新日期：1983-02-15 00:00:00

  • Increased myocardial short-range forces in a rodent model of diabetes reflect elevated content of β myosin heavy chain.

    abstract：:Diastolic dysfunction is a clinically significant problem for patients with diabetes and often reflects increased ventricular stiffness. Attached cross-bridges contribute to myocardial stiffness and produce short-range forces, but it is not yet known whether these forces are altered in diabetes. In this study, we test...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2013.08.013

    authors： Chung CS,Mitov MI,Callahan LA,Campbell KS

    更新日期：2014-06-15 00:00:00

  • Cloning, DNA sequencing, and amino acid sequencing of catechol 1,2-dioxygenases (pyrocatechase) from Pseudomonas putida mt-2 and Pseudomonas arvilla C-1.

    abstract：:Catechol 1,2-dioxygenase catalyzes the oxygenative ring cleavage of catechol to form cis,cis-muconic acid and is encoded by a catA gene. We have cloned a catA gene from Pseudomonas putida mt-2 using a PCR product of amino acid sequence-based primers as a probe. The amino acid sequence deduced from the 930 nucleotides ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1405

    authors： Nakai C,Uyeyama H,Kagamiyama H,Nakazawa T,Inouye S,Kishi F,Nakazawa A,Nozaki M

    更新日期：1995-08-20 00:00:00

  • Aldehyde dehydrogenase activity as the rate-limiting factor for acetaldehyde metabolism in rat liver.

    abstract：:The velocity of acetaldehyde metabolism in rat liver may be governed either by the rate of regeneration of NAD from NADH through the electron transport system or by the activity of aldehyde dehydrogenase (ALDH). Measurements of oxygen consumption revealed that the electron transport system was capable of reoxidizing A...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90603-4

    authors： Svanas GW,Weiner H

    更新日期：1985-01-01 00:00:00

  • Synthetic ceramides induce growth arrest or apoptosis by altering cellular redox status.

    abstract：:Reactive oxygen species (ROS) and ceramide are each partly responsible for the signal transduction of a variety of extracellular agents. Furthermore, the application of synthetic, short-chain ceramides mimics the cellular responses to these extracellular agents. However, the significance of ROS involvement in ceramide...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/s0003-9861(02)00496-4

    authors： Phillips DC,Allen K,Griffiths HR

    更新日期：2002-11-01 00:00:00

  • The effect of essential fatty acid deficiency on the adrenergic activation of glycogenolysis in rat hepatocytes.

    abstract：:The fatty acid composition of total lipids and the adrenoceptor-mediated activation of glycogenolysis were studied in isolated hepatocytes from rats maintained on a control diet or on an essential fatty acid (EFA)-free diet. In cells from rats on the EFA-free diet there was a marked reduction in linoleic and arachidon...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(90)90608-2

    authors： Grojec MS,Ishac EJ,Kapocsi J,Kunos G

    更新日期：1990-11-15 00:00:00

  • Oxidative metabolism in rat hepatocytes and mitochondria during sepsis.

    abstract：:We hypothesized that cellular oxygen consumption is abnormal during sepsis as a result of increased oxidative stress and selective mitochondrial damage. In a rat model of sepsis (cecal ligation and puncture), we studied the respiratory characteristics of isolated hepatocytes and liver mitochondria 16 h after onset of ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1997.0264

    authors： Kantrow SP,Taylor DE,Carraway MS,Piantadosi CA

    更新日期：1997-09-15 00:00:00

  • Relative activities of retrovirally expressed murine prostaglandin synthase-1 and -2 depend on source of arachidonic acid.

    abstract：:We have developed derivatives of mouse embryonic fibroblasts (10T1/2) and Chinese hamster ovary (AS52) cells that stably express high levels of murine prostaglandin synthase-1 or -2 (PGHS-1 or -2). The cDNAs were transferred using retroviral vectors and the resulting G418-resistant clones were analyzed for prostagland...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0257

    authors： Chulada PC,Loftin CD,Winn VD,Young DA,Tiano HF,Eling TE,Langenbach R

    更新日期：1996-06-15 00:00:00

  • IL-1β/NF-kb signaling promotes colorectal cancer cell growth through miR-181a/PTEN axis.

    abstract：:To date, the role of miRNA in tumorigenesis has been largely reported. It was found that miR-181a may be involved in the tumorigenesis of colon cancer. The purpose of this study was to investigate the mechanism of miR-181a in colon cancer carcinogenesis. The expression levels of IL-1β, NF-κB (RelA), and miR-181a in co...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2016.06.001

    authors： Hai Ping P,Feng Bo T,Li L,Nan Hui Y,Hong Z

    更新日期：2016-08-15 00:00:00

  • Purification of a thymidine-diphospho-4-keto-6-deoxy-D-glucose epimerase from an erythromycin-producing strain of Saccharopolyspora erythraea.

    abstract：:A thymidine-diphospho-4-keto-6-deoxy-D-glucose epimerase was purified from Saccharopolyspora erythraea, the producer of the macrolide antibiotic erythromycin, by a high resolution chromatographic method that exploited the difference in behavior of the protein on ion exchange columns at pH 7.5 and 5.5. By this procedur...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1025

    authors： Jarvis BW,Hutchinson CR

    更新日期：1994-01-01 00:00:00

  • Modification of an essential amino group of phosphoenolpyruvate carboxylase from maize leaves by pyridoxal phosphate and by pyridoxal phosphate-sensitized photooxidation.

    abstract：:Phosphoenolpyruvate carboxylase from maize leaves was inactivated by pyridoxal 5'-phosphate in the dark and in the light. A two-step reversible mechanism is proposed for inactivation in the dark, which involves the formation of a noncovalent complex prior to a Schiff base with amino groups of the enzyme. Spectral anal...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(86)90309-7

    authors： Podesta FE,Iglesias AA,Andreo CS

    更新日期：1986-05-01 00:00:00

  • Mechanism-based inactivation of human leukocyte elastase via an enzyme-induced sulfonamide fragmentation process.

    abstract：:We describe herein the design and in vitro biochemical evaluation of a novel class of mechanism-based inhibitors of human leukocyte elastase (HLE) that inactivate the enzyme via an unprecedented enzyme-induced sulfonamide fragmentation cascade. The inhibitors incorporate in their structure an appropriately functionali...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2004.05.018

    authors： Wei L,Lai Z,Gan X,Alliston KR,Zhong J,Epp JB,Tu J,Perera AB,Van Stipdonk M,Groutas WC

    更新日期：2004-09-01 00:00:00

  • Isolation and biochemical characterization of hemorrhagic toxin f from the venom of Crotalus atrox (western diamondback rattlesnake).

    abstract：:Hemorrhagic toxin f (HT-f) was isolated from Crotalus atrox (Western Diamondback Rattlesnake) venom by a five-step purification procedure. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing, and sodium dodecyl sulfate (SDS)-electrophoresis. HT-f has a ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90393-x

    authors： Nikai T,Mori N,Kishida M,Sugihara H,Tu AT

    更新日期：1984-06-01 00:00:00

  • Dulaglutide inhibits high glucose- induced endothelial dysfunction and NLRP3 inflammasome activation.

    abstract：:Activation of the NLRP3 inflammasome plays an important role in high glucose- induced endothelial dysfunction in patients with type 2 diabetes mellitus (T2DM). Dulaglutide, a newly developed glucagon-like peptide-1 receptor (GLP-1R) agonist, has been approved for the management of T2DM. In the current study, we aimed ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2019.07.008

    authors： Luo X,Hu Y,He S,Ye Q,Lv Z,Liu J,Chen X

    更新日期：2019-08-15 00:00:00

  • Partial characterization of nuclear binding sites for retinol delivered by cellular retinol binding protein.

    abstract：:Retinol (vitamin A alcohol), which plays an important role in the differentiation of epithelia, can be transferred to chromatin in vitro. Rat liver chromatin can accept retinol in a specific and saturable manner only when the retinol is presented as a complex with cellular retinol-binding protein (CRBP). A partial cha...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90287-5

    authors： Liau G,Ong DE,Chytil F

    更新日期：1985-03-01 00:00:00

  • S-adenosyl-L-methionine:trans-caffeoyl-coenzyme A 3-O-methyltransferase from elicitor-treated parsley cell suspension cultures.

    abstract：:An S-adenosyl-L-methionine:caffeoyl-CoA 3-O-methyltransferase was purified 82-fold from elicitor-induced parsley cell suspension cultures by ammonium sulfate fractionation, anionic exchange and hydrophobic interaction chromatographies, and chromatofocusing. The enzyme has an apparent pI of 5.7 and a molecular weight o...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90299-3

    authors： Pakusch AE,Kneusel RE,Matern U

    更新日期：1989-06-01 00:00:00

  • Effect of training on H(2)O(2) release by mitochondria from rat skeletal muscle.

    abstract：:In this study, oxygen consumption and H(2)O(2) release rate by succinate or pyruvate/malate supplemented mitochondria isolated from skeletal muscle of trained and untrained rats were investigated. The overall mitochondrial antioxidant capacity and the effect of preincubation of mitochondria with GDP, an inhibitor of u...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1494

    authors： Venditti P,Masullo P,Di Meo S

    更新日期：1999-12-15 00:00:00

  • Dose-dependent response of serum lutein and macular pigment optical density to supplementation with lutein esters.

    abstract：:We conducted a study to determine the effect of different doses of a lutein supplement on serum lutein concentration and macular pigment optical density (MPOD). Lutein is one of the major components of human macular pigment. Eighty-seven subjects received daily doses of 5, 10, or 20 mg of lutein, or a placebo, over a ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.abb.2010.06.019

    authors： Bone RA,Landrum JT

    更新日期：2010-12-01 00:00:00

  • Metabolism of NG,NG-and NG,N'G-dimethylarginine in rats.

    abstract：:The metabolic fates of NG,NG-and NG,N'G-dimethylarginines in rats were investigated isotopically and novel metabolites, alpha-keto-delta-(N,N-dimethylguanidino)-and alpha-keto-delta-(N,N'-dimethylguanidino)valeric acids and gamma-(N,N-dimethylguanidino)-and gamma-(N,N'-dimethylguanidino)butyric acids were identified. ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90060-9

    authors： Ogawa T,Kimoto M,Watanabe H,Sasaoka K

    更新日期：1987-02-01 00:00:00

  • Fatty acid binding protein: stimulation of microsomal phosphatidic acid formation.

    abstract：:The effect of fatty acid binding proteins (FABPs) on two key steps of microsomal phosphatidic acid formation was examined. Rat liver microsomes were purified by size-exclusion chromatography to remove endogenous cytosolic fatty acid and fatty acyl-CoA binding proteins while recombinant FABPs were used to avoid cross-c...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1997.9957

    authors： Jolly CA,Hubbell T,Behnke WD,Schroeder F

    更新日期：1997-05-01 00:00:00

  • Evidence for a radical mechanism in peroxidase-catalyzed coupling. I. Steady-state experiments with various peroxidases.

    abstract：:Biosynthesis of thyroxine in the thyroid gland involves a reaction between two diiodotyrosyl residues within the same molecule of thyroglobulin, a large, thyroid-specific glycoprotein. This reaction, generally referred to as the coupling reaction, is catalyzed in the thyroid by the heme-containing glycoprotein enzyme,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1474

    authors： Taurog A,Dorris M,Doerge DR

    更新日期：1994-11-15 00:00:00

  • Identification of a 42-kDa plant mitochondrial outer membrane protein, MOM42, involved in the import of precursor proteins into plant mitochondria.

    abstract：:A 42-kDa plant outer mitochondrial membrane protein, MOM42, has been identified as an essential component of the plant mitochondrial precursor protein translocation apparatus. Immunological cross-reactivity has been detected between antibodies raised against both Neurospora and yeast mitochondrial outer membrane prote...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1088

    authors： Perryman RA,Mooney B,Harmey MA

    更新日期：1995-02-01 00:00:00

  • Cobinding of bilirubin and sulfonamide and of two bilirubin molecules to human serum albumin: a site model.

    abstract：:Differential light absorption spectra of the bilirubin-albumin 1:1 complex, obtained on addition of 20 different sulfonamides, differ with respect to shape and amplitude. This finding seems to indicate that the sulfonamide molecule is bound in direct touch with the bilirubin. The light absorption spectrum of bilirubin...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90063-4

    authors： Brodersen R,Knudsen A,Pedersen AO

    更新日期：1987-02-01 00:00:00

  • Multiple replacements establish the importance of tyrosine-503 in beta-galactosidase (Escherichia coli).

    abstract：:Tyr-503 of beta-galactosidase was specifically replaced with Phe, His, Cys, and Lys using site-directed mutagenesis. The normal enzyme and the substituted enzymes were purified. The activities of each of the substituted enzymes with o-nitrophenyl-beta-D-galactopyranoside (ONPG) and p-nitrophenyl-beta-D-galactopyronosi...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(90)90652-f

    authors： Ring M,Huber RE

    更新日期：1990-12-01 00:00:00