Minimizing frustration by folding in an aqueous environment.

abstract：:PCNA is well known as a component of DNA replication system and plays important roles in multiple cellular pathways in addition to replication and repair. In this work we have demonstrated the physical and functional interaction between tobacco PCNA and mungbean ddNTP-sensitive DNA polymerase which shares many physico...

journal_title：Archives of biochemistry and biophysics

authors： Roy S,Roy Choudhury S,Mukherjee SK,Sengupta DN

更新日期：2007-12-01 00:00:00

abstract：:The zinc-binding motif (HELLGH) of dipeptidyl peptidase III (DPP III) is different from the common zinc-binding motif (HExxH) of metallopeptidases. To clarify the importance of the zinc-binding motif part of DPP III for enzymatic activity, we measured the recovery of the enzyme activity of apo-Leu(453)-deleted dipepti...

journal_title：Archives of biochemistry and biophysics

authors： Hirose J,Kamigakiuchi H,Iwamoto H,Fujii H,Nakai M,Takenaka M,Kataoka R,Sugahara M,Yamamoto S,Fukasawa KM

更新日期：2004-11-01 00:00:00

abstract：:Fructose 2,6-bisphosphate inhibited all three fructose-1,6-bisphosphatases from the liver, intestine, and muscle of the mouse. The sensitivity of the liver enzyme to the inhibitor was significantly diminished when Mg2+ was replaced by Mn2+ as the activating cation. Inhibition of the liver enzyme by fructose 2,6-bispho...

journal_title：Archives of biochemistry and biophysics

authors： Mizunuma H,Tashima Y

更新日期：1983-10-01 00:00:00

abstract：:Solubilized NADPH-cytochrome c (P450) reductase was purified to homogeneity from an extract of spearmint (Mentha spicata) glandular trichomes by dye-ligand interaction chromatography on Matrex-Gel Red A and affinity chromatography on 2', 5'-adenosine diphosphate agarose. SDS-PAGE of the purified enzyme preparation rev...

journal_title：Archives of biochemistry and biophysics

authors： Ponnamperuma K,Croteau R

更新日期：1996-05-01 00:00:00

abstract：:Inactivations of chicken liver pyruvate carboxylase with N-(7-dimethylamino-4-methyl-3-coumarinyl)maleimide (DACM) and o-phthalaldehyde (o-PA) have identified cysteine and lysine residues that are essential for catalytic activity. Protection experiments suggest that the modified residues are located in or near the fir...

journal_title：Archives of biochemistry and biophysics

authors： Werneburg BG,Ash DE

更新日期：1993-06-01 00:00:00

abstract：:We have studied the influence of D-amino acid substitution in the flanking region on the antibody recognition of the 19TGTQ22 epitope core in the tandem repeat of mucin 2 (MUC2) glycoprotein. Analogue peptides corresponding to the optimal epitope sequence (16PTPTGTQ22) have been prepared by the replacement of single o...

journal_title：Archives of biochemistry and biophysics

authors： Uray K,Kajtár J,Vass E,Price MR,Hollósi M,Hudecz F

更新日期：2000-06-01 00:00:00

abstract：:This work addresses a correlation between the redox state of pyridine nucleotides and that of sulfhydryl groups of the mitochondrial membranes. Several major observations emerge: (1) Conditions leading to an oxidation of the pyridine nucleotides such as incubation with tert-butyl hydroperoxide or acetoacetate determin...

journal_title：Archives of biochemistry and biophysics

authors： Bindoli A,Callegaro MT,Barzon E,Benetti M,Rigobello MP

更新日期：1997-06-01 00:00:00

abstract：:B4-2-1 cells (Lec15 cells) are Chinese hamster ovary cells deficient in mannosylphosphoryldolichol synthase activity. They synthesize the truncated lipid intermediate Man5GlcNAc2-P-P-dolichol rather than the Glc3Man9GlcNAc2-P-P-dolichol synthesized by wild-type cells. In this report we present evidence that these cell...

journal_title：Archives of biochemistry and biophysics

authors： Stoll J,Cacan R,Verbert A,Krag SS

更新日期：1992-12-01 00:00:00

abstract：:There is dose-dependent Cd(2+)-evoked swelling of isolated rat liver mitochondria energized by complex I, II, or IV respiratory substrates in sucrose medium in the absence of added Ca(2+) and P(i), which is prevented by Sr(2+). Permeability transition effectors (ADP, CsA, EGTA, RR, DTT, ATR, P(i), and Ca(2+)) affect i...

journal_title：Archives of biochemistry and biophysics

authors： Belyaeva EA,Glazunov VV,Korotkov SM

更新日期：2002-09-15 00:00:00

abstract：:The plant-derived flavonoid, quercetin (QCT), has many biological actions, including cardioprotective actions, resulting from its antioxidant and anti-inflammatory effects. In this study, effects of QCT and its metabolites on the contraction and Ca2+ transients (CaT) of mouse single cardiomyocytes were simultaneously ...

journal_title：Archives of biochemistry and biophysics

authors： Hayamizu K,Morimoto S,Nonaka M,Hoka S,Sasaguri T

更新日期：2018-01-01 00:00:00

abstract：:A 60- to 70-fold purification of an NAD+ glycohydrolase from the inner membrane of rat liver mitochondria to apparent homogeneity on sodium dodecyl sulfate (SDS)-polyacrylamide slab gel is described. The minimum molecular weight of the enzyme on polyacrylamide gels in the presence of SDS is around 62,000. The enzyme s...

journal_title：Archives of biochemistry and biophysics

authors： Moser B,Winterhalter KH,Richter C

更新日期：1983-07-01 00:00:00

abstract：:The effect of hydroperoxides on hematin-catalyzed initiation and propagation of lipid peroxidation was examined utilizing soybean phosphatidylcholine liposomes as model membranes. Polarographic and spectrophotometric methods revealed a bimodal pseudocatalytic activity for hematin. A slow initiation phase of peroxidati...

journal_title：Archives of biochemistry and biophysics

authors： Kim EH,Sevanian A

更新日期：1991-08-01 00:00:00

abstract：:If liver alcohol dehydrogenase were rate-limiting in ethanol metabolism, inhibitors of the enzyme should inhibit the metabolism with the same type of kinetics and the same kinetic constants in vitro and in vivo. Against varied concentrations of ethanol, 4-methylpyrazole is a competitive inhibitor of purified rat liver...

journal_title：Archives of biochemistry and biophysics

authors： Plapp BV,Leidal KG,Smith RK,Murch BP

更新日期：1984-04-01 00:00:00

abstract：:The rotating frame nuclear magnetic resonance relaxation rate R(1rho) in the blood and cell lysate was studied at 4.7T to provide reference values for in vivo modeling and to address the mechanisms contributing to net relaxation. A strong dependence on oxygenation, hematocrit, and spin lock field strength B(1) (0.2-1....

journal_title：Archives of biochemistry and biophysics

authors： Johanna Silvennoinen M,Kettunen MI,Clingman CS,Kauppinen RA

更新日期：2002-09-01 00:00:00

abstract：:We investigated the role of free radicals in hemoglobin (Hb) oxidation and denaturation. To generate free radicals, we used two azocompounds, the hydrophilic 2,2'-azobis(2-amidinopropane hydrochloride and the hydrophobic 2,2'-azobis(2,4-dimethylvaleronitrile) and a drug of the quinone family, phenazine methosulfate. T...

journal_title：Archives of biochemistry and biophysics

authors： Minetti M,Mallozzi C,Scorza G,Scott MD,Kuypers FA,Lubin BH

更新日期：1993-04-01 00:00:00

abstract：:Experimental autoimmune encephalomyelitis (EAE) is a commonly used mouse model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS) characterized by demyelination leading to brain and spinal cord malfunctions. We postulate that not only biological but also biomechanical properties ...

journal_title：Archives of biochemistry and biophysics

authors： Pyka-Fościak G,Zemła J,Lis GJ,Litwin JA,Lekka M

更新日期：2020-02-15 00:00:00

abstract：:In trout and mammals, the major extrahepatic expression site for CYP3A forms is in the intestine. A cDNA encoding a new CYP3A subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (RT-PCR), followed by rapid amplification of cDNA ends (RACE)-PCR. In a set o...

journal_title：Archives of biochemistry and biophysics

authors： Lee SJ,Buhler DR

更新日期：2003-04-01 00:00:00

abstract：:We studied the effect of KAR-2 on cytosolic Ca(2+) level in human neutrophils by using a fluorescent dye (Fura-2) trapped in the cells. KAR-2 is a semisynthetic bis-indole derivative that shares vinblastine anti-microtubular properties, but does not share the vinblastine antagonistic effect on calmodulin. Therefore KA...

journal_title：Archives of biochemistry and biophysics

authors： Salerno C,Capuozzo E

更新日期：2013-09-01 00:00:00

abstract：:A number of early experiments suggested a relationship between methyl group metabolism and the exocrine secretion of the pancreas. These included nutritional studies showing that ethionine, the ethyl analog of methionine which inhibits cellular methylation reactions, is a specific pancreatic toxin. Other studies indic...

journal_title：Archives of biochemistry and biophysics

authors： Capdevila A,Decha-Umphai W,Song KH,Borchardt RT,Wagner C

更新日期：1997-09-01 00:00:00

abstract：:The ionophore properties of cationomycin and monensin were studied on human erythrocytes by measuring Na+ influx by 23Na NMR and concomitant K+ efflux by potentiometry in the presence of increasing amounts of serum. Both ion currents (Na+ or K+) decreased linearly with the reciprocal of serum amount. The serum effects...

journal_title：Archives of biochemistry and biophysics

authors： Gibot S,Jeminet G,Juillard J,Gumila C,Ancelin ML,Vial H,Delort AM

更新日期：1999-03-15 00:00:00

abstract：:The important characteristic of novel Mimosa pudica tubulin is its ability to bind colchicine only when dithiothreitol is included in the isolation buffer, indicating the involvement of sulfhydryl groups in colchicine binding. Modification of sulfhydryl groups by a sulfhydryl modifying agent also affects the normal as...

journal_title：Archives of biochemistry and biophysics

authors： Roychaudhuri A,Biswas S

更新日期：1992-05-01 00:00:00

abstract：:NahR, a member of the LysR regulator family, is a positive transcriptional regulator for genes of the naphthalene degradation pathway in Pseudomonas sp. To study NahR binding properties, five single and six double mutants were made at residues 169 and/or 248, which are located in the central inducer recognition domain...

journal_title：Archives of biochemistry and biophysics

authors： Park HH,Lee HY,Lim WK,Shin HJ

更新日期：2005-02-01 00:00:00

abstract：:Structural aspects of the binding of the linear ligands N alpha-acetyl-L-lysine (AcLys) and epsilon-aminocaproic acid (epsilon ACA) and of the cyclic analogs trans-(aminomethyl)-cyclohexanecarboxylic acid (AMCHA) and p-benzylaminesulfonic acid (BASA) to the intact plasminogen kringle 4 domain have been investigated by...

journal_title：Archives of biochemistry and biophysics

authors： De Marco A,Laursen RA,Llinas M

更新日期：1986-02-01 00:00:00

abstract：:The thermodynamic parameters of the alkaline transition for oxidized native yeast iso-1 cytochrome c and Rhodopseudomonas palustris cytochrome c(2) (cytc(2)) have been determined through direct electrochemistry experiments carried out at variable pH and temperature and compared to those for horse and beef heart cytoch...

journal_title：Archives of biochemistry and biophysics

authors： Battistuzzi G,Borsari M,Ranieri A,Sola M

更新日期：2002-08-15 00:00:00

abstract：:Deoxycholic acid, a colon tumor promoter, was found to bind covalently to DNA and RNA in the presence of methyl linoleate hydroperoxide and ferrous ion. This binding was shown to occur specifically with guanine residues and its covalent nature was demonstrated by analysis of hydrolysates of nucleic acid adducts. These...

journal_title：Archives of biochemistry and biophysics

authors： Kodama M,Inoue F,Nakayama T,Nagata C

更新日期：1987-11-01 00:00:00

abstract：:To elucidate the physiological significance of ketone bodies on insulin and glucagon secretion, the direct effects of beta-hydroxybutyrate (BOHB) and acetoacetate (AcAc) infusion on insulin and glucagon release from perfused rat pancreas were investigated. The BOHB or AcAc was administered at concentrations of 10, 1, ...

journal_title：Archives of biochemistry and biophysics

authors： Ikeda T,Yoshida T,Ito Y,Murakami I,Mokuda O,Tominaga M,Mashiba H

更新日期：1987-08-15 00:00:00

abstract：:The earthworm-derived chemoattractant ES20 interacts with its G-protein-coupled receptors on the plasma membrane of vomeronasal (VN) sensory neurons of garter snakes, resulting in an increase in inositol trisphosphate [J. Biol. Chem. 269 (1994) 16867] and a rapid phosphorylation of the membrane-bound proteins, p42/44 ...

journal_title：Archives of biochemistry and biophysics

authors： Wang D,Liu W,Liu J,Chen P,Quan W,Halpern M

更新日期：2002-12-15 00:00:00

abstract：:Oxidation of [2,3-14C]succinate carbons in the mitochondrial Krebs cycle was used as a probe to investigate the effects of insulin, epinephrine, glucagon, and 2,4-dinitrophenol (2,4-DNP) on isolated rat hepatocytes. Epinephrine, glucagon, and 2,4-DNP had a far greater stimulatory effect on 14CO2 formation from [2,3-14...

journal_title：Archives of biochemistry and biophysics

authors： Mohan C,Memon RA,Bessman SP

更新日期：1991-05-15 00:00:00

abstract：:Rabbit antibodies raised against the hydrophilic part of microsomal NADPH-cytochrome P450 oxidoreductase (denoted fpT) demonstrated a marked ability to inhibit NADPH-sterol Delta7-reductase activity. In addition, trypsin and proteinase K treatment of microsomes removed almost all microsomal electron transfer constitue...

journal_title：Archives of biochemistry and biophysics

authors： Nishino H,Ishibashi T

更新日期：2000-02-15 00:00:00

abstract：:Thromboxane A2 synthase (TXAS) binds to the endoplasmic reticulum membrane and catalyzes both an isomerization of prostaglandin H2 (PGH2) to form thromboxane A2 (TXA2) and a fragmentation of PGH2 to form 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) and malondialdehyde (MDA). TXAS is a non-classic cytochrome P450 in ...

journal_title：Archives of biochemistry and biophysics

authors： Hsu PY,Wang LH

更新日期：2003-08-01 00:00:00