The asymmetric orientation of cytochrome b561 in bovine chromaffin granule membranes.

abstract：:The cationic protein, lysozyme, is present in cartilage but its precise role in this tissue has not yet been established. This study shows that two major and structurally similar glycosaminoglycans (GAGs) of cartilage, i.e., chondroitin sulfate (CS) and hyaluronan (HA) interact with lysozyme at salt concentrations up ...

journal_title：Archives of biochemistry and biophysics

authors： Van Damme MP,Moss JM,Murphy WH,Preston BN

更新日期：1994-04-01 00:00:00

abstract：:Much effort has focused on the identification of MAPK cascades that are activated by the MEKK family of protein kinases. However, direct phosphorylation and regulation of the MEKK proteins has not been shown. To address this question, we have expressed recombinant (His)6FLAG.MEKK3 in Sf9 insect cells and tethered the ...

journal_title：Archives of biochemistry and biophysics

authors： Adams DG,Sachs NA,Vaillancourt RR

更新日期：2002-11-01 00:00:00

abstract：:Pseudonaja textilis, an Australian Elapid, is known to produce a highly toxic venom. Both protein profiling and N-terminal sequence analysis showed the presence of four new phospholipases A(2) in this venom. Besides being non-lethal, the phospholipase A(2) proteins were found to be moderately active enzymes and they s...

journal_title：Archives of biochemistry and biophysics

authors： Armugam A,Gong N,Li X,Siew PY,Chai SC,Nair R,Jeyaseelan K

更新日期：2004-01-01 00:00:00

abstract：:The ionization of 4-nitroimidazole to 4-nitroimidazolate was investigated as a function of ionic strength. The apparent pKa varies from 8.99 to 9.50 between 0.001 and 1.0 M ionic strength, respectively, at 25 degrees C. The ionic strength dependence of this ionization is anomalous. The binding of 4-nitroimidazole by h...

journal_title：Archives of biochemistry and biophysics

authors： Taylor KC,Vitello LB,Erman JE

更新日期：2000-10-15 00:00:00

abstract：:Recent analyses of the orange, red, and purple plumages of cotingas (Cotingidae) and broadbills (Eurylaimidae) revealed the presence of novel carotenoid molecules, suggesting that the diversity of pigments and the metabolic transformations they undergo are not yet fully understood. Two Old World orioles, the Black-and...

journal_title：Archives of biochemistry and biophysics

authors： LaFountain AM,Frank HA,Prum RO

更新日期：2013-11-15 00:00:00

abstract：:Modification/chlorination of high-density lipoprotein (HDL) by hypochlorous acid (HOCl), formed by the myeloperoxidase-H₂O₂-chloride system of activated phagocytes, converts an anti-atherogenic lipoprotein into a pro-inflammatory lipoprotein particle. Chlorinated HDL is present in human lesion material, binds to and i...

journal_title：Archives of biochemistry and biophysics

authors： Rossmann C,Rauh A,Hammer A,Windischhofer W,Zirkl S,Sattler W,Malle E

更新日期：2011-05-01 00:00:00

abstract：:Incubation of rat liver nuclear envelopes with [gamma-32P]ATP resulted in the synthesis of phosphatidylinositol-[4-32P]phosphate (PIP). Degradation of endogenously labeled PIP was observed upon the dilution of the labeled ATP with an excess of unlabeled ATP. This degradation was most rapid in the presence of EDTA, and...

journal_title：Archives of biochemistry and biophysics

authors： Smith CD,Wells WW

更新日期：1984-12-01 00:00:00

abstract：:Sarcolemmal vesicles of white and red skeletal muscles of the rabbit were prepared by consecutive density gradient centrifugations in sucrose and dextran according to Seiler and Fleischer (1982, J. Biol. Chem. 257, 13,862-13,871). White and red muscle membrane fractions enriched in sarcolemma were characterized by hig...

journal_title：Archives of biochemistry and biophysics

authors： Wetzel P,Gros G

更新日期：1990-06-01 00:00:00

abstract：:Periodate treatments of apo human serum transferrin (HST), and apo chicken ovotransferrin (COT) were previously reported to cause a rapid loss of Fe+3 binding capacity, with a loss of 3 to 5 tyrosine residues [P. AZARI AND J. L. PHILLIPS (1970) Arch. Biochem. Biophys. 138, 32-38; K. F. GEOGHEGAN, J. L. DALLAS, AND R. ...

journal_title：Archives of biochemistry and biophysics

authors： Penner MH,Yamasaki RB,Osuga DT,Babin DR,Meares CF,Feeney RE

更新日期：1983-09-01 00:00:00

abstract：:The effects of various metabolites on pyruvate dehydrogenase (PDH) kinase-catalyzed inactivation of the pyruvate dehydrogenase complex (PDC) were studied in extracts of mitochondria purified from green leaf tissue of Pisum sativum L. Pyruvate was an uncompetitive inhibitor of PDH kinase with respect to ATP whereas ADP...

journal_title：Archives of biochemistry and biophysics

authors： Schuller KA,Randall DD

更新日期：1990-04-01 00:00:00

abstract：:Methionine is an essential amino acid for both prokaryotic and eukaryotic organisms; however, little is known concerning its utilization in African trypanosomes, protozoa of the Trypanosoma brucei group. This study explored the Michaelis-Menten kinetic constants for transport and pool formation as well as metabolic ut...

journal_title：Archives of biochemistry and biophysics

authors： Goldberg B,Rattendi D,Lloyd D,Yarlett N,Bacchi CJ

更新日期：2000-05-01 00:00:00

abstract：:Two isoforms of regulatory (R) subunit of cAMP-dependent protein kinase (PKA), named R(myt1) and R(myt2), were identified so far in the sea mussel Mytilus galloprovincialis. Out of them, only R(myt2) was phosphorylated in vitro by casein kinase 2 (CK2) using GTP as phosphate donor. CK2 catalytic subunit (CK2alpha) its...

journal_title：Archives of biochemistry and biophysics

authors： Bardales JR,Hellman U,Villamarín JA

更新日期：2007-05-01 00:00:00

abstract：:The direct membrane effect of propranolol was studied in vitro on washed platelet preparations. Propranolol has been reported to inhibit platelet aggregation by mechanisms unrelated to its beta-blocking activity. In the present study, the drug was found to enhance 1-anilino-8-naphthalene sulfonate binding to platelet ...

journal_title：Archives of biochemistry and biophysics

authors： Dash D,Rao GR

更新日期：1990-02-01 00:00:00

abstract：:Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase [ATP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.49] is completely inactivated by the 2',3'-dialdehyde derivative of ATP (oATP) in the presence of Mn2+. The dependence of the pseudo-first-order rate constant on reagent concentration indicates t...

journal_title：Archives of biochemistry and biophysics

authors： Saavedra C,Araneda S,Cardemil E

更新日期：1988-11-15 00:00:00

abstract：:Nitric oxide (NO) is involved in angiogenesis and stimulates the EGF-R signaling pathway. Stimulation of different endothelial cell lines with bradykinin (BK) activates the endothelial NO synthase (eNOS) and promotes EGF-R tyrosine phosphorylation. Increase in NO production correlated with enhanced phosphorylation of ...

journal_title：Archives of biochemistry and biophysics

authors： Moraes MS,Costa PE,Batista WL,Paschoalin T,Curcio MF,Borges RE,Taha MO,Fonseca FV,Stern A,Monteiro HP

更新日期：2014-09-15 00:00:00

abstract：:Because angiogenesis underlies the pathogenesis of numerous conditions (cancer, psoriasis, macular degeneration), there is a pressing need for continued investigations into angiogenic signaling and potential drug targets. Antiangiogenic agents can be classified as either direct or indirect. Direct antiangiogenics act ...

journal_title：Archives of biochemistry and biophysics

authors： Berrios RL,Arbiser JL

更新日期：2011-04-15 00:00:00

abstract：:Purified human lecithin:cholesterol acyltransferase (LCAT) was covalently labeled by [3H]diisopropylflourophosphate with concomitant loss of enzymatic activity (M. Jauhiainen and P.J. Dolphin (1986) J. Biol. Chem. 261, 7023-7043). Some 60% of the enzyme was labeled in 1 h. Cyanogen bromide (CNBr) cleavage of the label...

journal_title：Archives of biochemistry and biophysics

authors： Farooqui JZ,Wohl RC,Kézdy FJ,Scanu AM

更新日期：1988-03-01 00:00:00

abstract：:The glucose transporter of the bacterial phosphotransferase system mediates sugar transport across the cytoplasmic membrane concomitant with sugar phosphorylation. It consists of a cytoplasmic subunit IIA(Glc) and the transmembrane subunit IICB(Glc). IICB(Glc) was purified to homogeneity by urea/alkali washing of memb...

journal_title：Archives of biochemistry and biophysics

authors： Zhuang J,Gutknecht R,Flükiger K,Hasler L,Erni B,Engel A

更新日期：1999-12-01 00:00:00

abstract：:Diphtheria toxin (DT) induces the leakage of dipalmitoylphosphatidic acid (DPPA) membranes but not neutral dipalmitoylphosphatidylcholine (DPPC) membranes. Cholesterol incorporated into liposomes enhances the membrane leakage induced by DT in acidic DPPA membranes but not in neutral DPPC membranes. Membrane leakage wa...

journal_title：Archives of biochemistry and biophysics

authors： Lai CS,Kushnaryov V,Panz T,Basosi R

更新日期：1984-10-01 00:00:00

abstract：:The G1 glycoprotein of Punta Toro virus, a member of the bunyavirus family, accumulates in the Golgi complex when it is expressed from cloned cDNA. We previously reported that the information necessary for Golgi retention of the G1 protein is located within the transmembrane domain and a portion of the cytoplasmic dom...

journal_title：Archives of biochemistry and biophysics

authors： Matsuoka Y,Chen SY,Holland CE,Compans RW

更新日期：1996-12-01 00:00:00

abstract：:The aim of this study was to investigate the effect of insulin resistance on glycogen concentration and glycogen synthase activity in the red and white gastrocnemius muscles and to determine whether the inverse relationship existing between glycogen concentration and enzyme activity is maintained in insulin resistant ...

journal_title：Archives of biochemistry and biophysics

authors： Coderre L,Vallega GA,Pilch PF,Chipkin SR

更新日期：2007-08-01 00:00:00

abstract：:Three alanine aminotransferases, two minor (AlaAT-1, AlaAT-3) and one major (AlaAT-2), were detected by native gel electrophoresis of leaf extracts from Panicum miliaceum L. AlaAT-2 was purified to homogeneity and a specific polyclonal antibody was raised against it which did not react with the other two forms of the ...

journal_title：Archives of biochemistry and biophysics

authors： Son D,Jo J,Sugiyama T

更新日期：1991-09-01 00:00:00

abstract：:Cellular glutathione is released during apoptosis and may play a role in the regulation of the mitochondrial permeability transition pore. The question of whether only cytosolic glutathione is important in apoptosis, or whether mitochondrial glutathione also plays a role, was investigated using gamma-glutamyltranspept...

journal_title：Archives of biochemistry and biophysics

authors： Will Y,Kaetzel RS,Brown MK,Fraley TS,Reed DJ

更新日期：2002-01-15 00:00:00

abstract：:Thermal perturbation techniques have been used to probe structural features of the nicotinic acetylcholine receptor (AcChR). The information obtained from differential scanning calorimetry (DSC) of AcChR membranes (M.C. Farach and M. Martinez-Carrion (1983) J. Biol. Chem. 258, 4176) in the absence and in the presence ...

journal_title：Archives of biochemistry and biophysics

authors： Artigues A,Villar MT,Ferragut JA,Gonzalez-Ros JM

更新日期：1987-10-01 00:00:00

abstract：:The soluble form of mitochondrial adenosine triphosphatase was purified in an electrophoretically and immunologically pure form from sweet potato root tissue. The enzyme consisted of six kinds of subunits with different molecular weights (52,500, 51,500, 35,500, 26,000, 23,000, and 12,000), and its molecular weight wa...

journal_title：Archives of biochemistry and biophysics

authors： Iwasaki Y,Asahi T

更新日期：1983-11-01 00:00:00

abstract：:The biosynthesis of dermatan sulfate is a complex process that involves, inter alia, formation of L-iduronic acid residues by C5-epimerization of D-glucuronic acid residues already incorporated into the growing polymer. It has been shown previously that this reaction is promoted by the presence of the sulfate donor 3'...

journal_title：Archives of biochemistry and biophysics

authors： Eklund E,Rodén L,Malmström M,Malmström A

更新日期：2000-11-15 00:00:00

abstract：:An inhibitor of the transforming growth factor-induced growth in soft agar of normally anchorage-dependent rat kidney fibroblasts has been detected in the acid-ethanol extracts of human placenta, bovine lung and kidney, and human rhabdomyosarcoma cells (A673). The inhibitor has been purified from human placenta by gel...

journal_title：Archives of biochemistry and biophysics

authors： Frolik CA,Roller PP,Cone JL,Dart LL,Smith DM,Sporn MB

更新日期：1984-04-01 00:00:00

abstract：:Production of hydroxyl radicals (HO.) by substrate-supplemented beef heart submitochondrial particles was studied by electron paramagnetic resonance in conjunction with the spin trap 5,5'-dimethyl-1-pirroline-N-oxide (DMPO). Supplementation of submitochondrial particles with NADH or succinate in the presence of antimy...

journal_title：Archives of biochemistry and biophysics

authors： Giulivi C,Boveris A,Cadenas E

更新日期：1995-02-01 00:00:00

abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

journal_title：Archives of biochemistry and biophysics

authors： Mohan SK,Rani SG,Chiu IM,Yu C

更新日期：2012-06-05 00:00:00

abstract：:A new procedure for the purification of phospholipase C from Clostridium perfringens has been devised that results in essentially pure enzyme. The procedure consists of ammonium sulfate fractionation, ion-exchange chromatography on QAE-Sephadex, and affinity chromatography on phosphatidylcholine linked to Sepharose. T...

journal_title：Archives of biochemistry and biophysics

authors： Krug EL,Kent C

更新日期：1984-06-01 00:00:00