Hypochlorite-modified high-density lipoprotein promotes induction of HO-1 in endothelial cells via activation of p42/44 MAPK and zinc finger transcription factor Egr-1.


:Modification/chlorination of high-density lipoprotein (HDL) by hypochlorous acid (HOCl), formed by the myeloperoxidase-H₂O₂-chloride system of activated phagocytes, converts an anti-atherogenic lipoprotein into a pro-inflammatory lipoprotein particle. Chlorinated HDL is present in human lesion material, binds to and is internalized by endothelial cells and impairs expression and activity of endothelial nitric oxide synthase. The present study aimed at clarifying whether exposure of endothelial cells to pro-inflammatory HOCl-HDL impacts on expression of heme oxygenase-1, a potential rescue pathway against endothelial dysfunction. Our findings revealed that HDL modified by HOCl, added as reagent or generated enzymatically, induced phosphorylation of p42/44 mitogen-activated protein kinase, expression of transcription factor early growth response-1 (Egr-1) and enhanced expression of heme oxygenase-1 in human endothelial cells. Upregulation of heme oxygenase-1 could be blocked by an inhibitor upstream of p42/44 mitogen-activated protein kinase and/or knockdown of Egr-1 by RNA-interference. Electrophoretic mobility shift assays demonstrated HOCl-HDL-mediated induction of the Egr-1 DNA binding activity. Immunocytochemical and immunoblotting experiments demonstrated HOCl-HDL-induced translocation of Egr-1 to the nucleus. The present study demonstrates a novel compensatory pathway against adverse effects of HOCl-HDL, providing cytoprotection in a number of pathological conditions including cardiovascular disease.


Arch Biochem Biophys


Rossmann C,Rauh A,Hammer A,Windischhofer W,Zirkl S,Sattler W,Malle E




Has Abstract


2011-05-01 00:00:00














  • Phosphorylation of rabbit skeletal muscle glycogen synthase by casein kinase 1: evidence of phosphorylation sites specific for casein kinase 1.

    abstract::Casein kinase 1 phosphorylated rabbit skeletal muscle glycogen synthase at both seryl and threonyl residues. With glycogen synthase phosphorylated up to 7.5 mol phosphate/mol subunit, about 26% of the phosphate was present in the N-terminal cyanogen bromide fragment (CB1) and 74% in the C-terminal fragment (CB2). Both...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Guasch MD,Plana M,Vila J,Itarte E

    更新日期:1986-11-01 00:00:00

  • Accumulation of various N-acylethanolamines including N-arachidonoylethanolamine (anandamide) in cadmium chloride-administered rat testis.

    abstract::Changes in the levels of various molecular species of N-acylethanolamine in CdCl2-administered rat testis were examined. We found that the levels of various N-acylethanolamines including anandamide (N-arachidonoylethanolamine), an endogenous cannabinoid receptor ligand, were dramatically increased in CdCl2-admin-ister...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kondo S,Sugiura T,Kodaka T,Kudo N,Waku K,Tokumura A

    更新日期:1998-06-15 00:00:00

  • Inhibition and reconstitution of Ah receptor transformation in vitro: role and partial characterization of a cytosolic factor(s).

    abstract::The Ah receptor binds 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related aryl hydrocarbons and mediates their biochemical and toxic effects by modifying gene expression. In order to interact with DNA, the TCDD.receptor complex must undergo a poorly understood transformation to a form which is distinguishable by it...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Henry EC,Gasiewicz TA

    更新日期:1991-07-01 00:00:00

  • Microsomally associated heme proteins from French bean: characterization of the cytochrome P450 cinnamate-4-hydroxylase and two peroxidases.

    abstract::Three heme-containing proteins of M(r) 57,000, 46,000, and 42,000 have been isolated from microsomes of elicitor-treated suspension-cultured cells of French bean. Following purification to homogeneity they were shown to be distinct with respect to amino acid and sugar compositions. The M(r) 57,000 protein proved to be...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Rodgers MW,Zimmerlin A,Werck-Reichhart D,Bolwell GP

    更新日期:1993-07-01 00:00:00

  • Metabolic biomarkers for chronic kidney disease.

    abstract::Chronic kidney disease (CKD) is an increasingly recognized burden for patients and health care systems with high (and growing) global incidence and prevalence, significant mortality, and disproportionately high treatment costs. Yet, the available diagnostic tools are either impractical in clinical routine or have seri...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Breit M,Weinberger KM

    更新日期:2016-01-01 00:00:00

  • Modulation of the activity of NAD malic enzyme from solanum tuberosum by changes in oligomeric state.

    abstract::The effects of pH, NaCl, and malate2- on the equilibrium between dimeric and higher-molecular-weight forms of NAD malic enzyme from Solanum tuberosum var. Chieftain have been analyzed by monitoring the kinetic changes associated with disaggregation [S. D. Grover and R. T. Wedding (1982) Plant Physiol. 70, 1169-1172]. ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Grover SD,Wedding RT

    更新日期:1984-11-01 00:00:00

  • Biosynthesis of 3-hydroxy fatty acids, the pheromone components of female mallard ducks, by cell-free preparations from the uropygial gland.

    abstract::Diesters of 3-hydroxy C8, C10, and C12 acids, the female mallard duck pheromones, were found as the major products of the uropygial glands only during the breeding season. The 3-hydroxy acids were identified by mass spectrometry of the trimethylsilyl ethers of the methyl esters and of the diols derived from LiAlH4 red...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kolattukudy PE,Rogers L

    更新日期:1987-01-01 00:00:00

  • Role of vitamin E in ascorbate-dependent protein thiol oxidation in rat liver endoplasmic reticulum.

    abstract::Addition of ascorbate or its generation from gulonolactone causes the oxidation of protein thiols and a simultaneous dehydroascorbate formation in rat liver microsomes. The participation of vitamin E in the phenomenon was studied. We measured ascorbate and protein thiol oxidation and lipid peroxidation in vitamin E de...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Csala M,Szarka A,Margittai E,Mile V,Kardon T,Braun L,Mandl J,Bánhegyi G

    更新日期:2001-04-01 00:00:00

  • Reversible thermal denaturation of staphylococcal nuclease: a Fourier transformed infrared spectrum study.

    abstract::The secondary structures of staphylococcal nuclease (SNase) have been assigned and semiquantitatively estimated from the deconvoluted Fourier transformed infrared (FTIR) spectrum. The changes in the secondary structures accompanying unfolding and refolding of SNase during reversible thermal denaturation up to 70 degre...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Xie L,Jing GZ,Zhou JM

    更新日期:1996-04-01 00:00:00

  • Characterization of tryptic hydrolysis of alpha-lactalbumin/saponin mixture and structural change of alpha-lactalbumin interacting with soybean saponin.

    abstract::Bovine milk alpha-lactalbumin (alpha-La) was mixed with soybean saponin, and the resulting mixture was hydrolyzed by trypsin. Saponin increased the tryptic-hydrolysis level of alpha-La only at relatively high phosphate buffer concentrations (> or = 0.05 M). T(1) experiments with acetylated soybean saponin demonstrated...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Shimoyamada M,Okada Y,Watanabe K,Yamauchi R

    更新日期:2005-03-15 00:00:00

  • Cloning, tissue distribution, and functional studies of a new cytochrome P450 3A subfamily member, CYP3A45, from rainbow trout (Oncorhynchus mykiss) intestinal ceca.

    abstract::In trout and mammals, the major extrahepatic expression site for CYP3A forms is in the intestine. A cDNA encoding a new CYP3A subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (RT-PCR), followed by rapid amplification of cDNA ends (RACE)-PCR. In a set o...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lee SJ,Buhler DR

    更新日期:2003-04-01 00:00:00

  • Regulation of mitochondrial sn-glycerol-3-phosphate acyltransferase activity: response to feeding status is unique in various rat tissues and is discordant with protein expression.

    abstract::Triacylglycerol plays a critical role in an organism's ability to withstand fuel deprivation, and dysregulation of triacylglycerol synthesis is important in the development of diseases such as obesity and diabetes. Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the initial and committed step of glycerolipid syn...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lewin TM,Granger DA,Kim JH,Coleman RA

    更新日期:2001-12-01 00:00:00

  • Tyrosine-phosphorylated calmodulin has reduced biological activity.

    abstract::Calmodulin is phosphorylated by the purified insulin receptor on tyrosine residues with a maximum stoichiometry of 1 mol phosphate/mol of calmodulin. Isolated tryptic phosphopeptides were sequenced by manual Edman degradation and demonstrated that calmodulin is equally phosphorylated on tyrosine 99 and tyrosine 138. P...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Williams JP,Jo H,Sacks DB,Crimmins DL,Thoma RS,Hunnicutt RE,Radding W,Sharma RK,McDonald JM

    更新日期:1994-11-15 00:00:00

  • Targeting thioredoxin reductase by plumbagin contributes to inducing apoptosis of HL-60 cells.

    abstract::Plumbagin (PLB), a natural naphthoquinone from the traditional folk medicines Plumbago zeylanica, Dionaea muscipula, or Nepenthes gracilis, has been documented possessing a wide variety of pharmacological activities. Although PLB demonstrates anticancer activity in multiple types of malignant cells, the cellular targe...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zhang J,Peng S,Li X,Liu R,Han X,Fang J

    更新日期:2017-04-01 00:00:00

  • Purification and characterization of an isozyme of catalase with enhanced-peroxidatic activity from leaves of Nicotiana sylvestris.

    abstract::Two isozymes of catalase (EC, one with typically low peroxidatic activity (CAT-1) and the other with enhanced-peroxidatic activity (EP-CAT or CAT-3) have been purified to electrophoretic homogeneity from tobacco (Nicotiana sylvestris) seedlings and antibodies prepared against each. The isozyme proteins showe...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Havir EA,McHale NA

    更新日期:1990-12-01 00:00:00

  • Interleukin-2 is one of the targets of 1,25-dihydroxyvitamin D3 in the immune system.

    abstract::Interleukin (IL)-2 knockout (KO) mice, which spontaneously develop symptoms of inflammatory bowel disease similar to ulcerative colitis in humans, were made vitamin D deficient (D-) or vitamin D sufficient (D+) or were supplemented with 1,25-dihydroxyvitamin D(3) (1,25D3). 1,25-Dihydroxyvitamin D3 supplementation, but...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Bemiss CJ,Mahon BD,Henry A,Weaver V,Cantorna MT

    更新日期:2002-06-15 00:00:00

  • Cysteine 98 in CYP3A4 contributes to conformational integrity required for P450 interaction with CYP reductase.

    abstract::Previously human cytochrome P450 3A4 was efficiently and specifically photolabeled by the photoaffinity ligand lapachenole. One of the modification sites was identified as cysteine 98 in the B-C loop region of the protein [B. Wen, C.E. Doneanu, C.A. Gartner, A.G. Roberts, W.M. Atkins, S.D. Nelson, Biochemistry 44 (200...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wen B,Lampe JN,Roberts AG,Atkins WM,David Rodrigues A,Nelson SD

    更新日期:2006-10-01 00:00:00

  • Alterations of heme, cytochrome P-450, and steroid metabolism by mercury in rat adrenal.

    abstract::The treatment of male rats with Hg2+ resulted in significant alterations in heme and hemoprotein metabolism in the adrenal gland which, in turn, were reflected in abnormal steroidogenic activities and steroid output. Twenty-four hours after the administration of 30 mumol of HgCl2/kg (sc) the mitochondrial heme and cyt...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Veltman JC,Maines MD

    更新日期:1986-08-01 00:00:00

  • Abnormal membrane properties of the sarcoplasmic reticulum of pigs susceptible to malignant hyperthermia: modes of action of halothane, caffeine, dantrolene, and two other drugs.

    abstract::The role of sarcoplasmic reticulum (SR) in malignant hyperthermia (MH) was studied using the heavy microsomal fraction prepared from semitendinosus muscles of both normal and genetically MH-susceptible pigs. In the presence of ATP, SR was loaded with 70 nmol Ca2+/mg SR protein. Under these conditions, MH-SR demonstrat...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ohnishi ST,Waring AJ,Fang SR,Horiuchi K,Flick JL,Sadanaga KK,Ohnishi T

    更新日期:1986-06-01 00:00:00

  • MicroRNA-130a has pro-fibroproliferative potential in hypertrophic scar by targeting CYLD.

    abstract::Hypertrophic scars are dermal fibrosis diseases that protrude from the surface of the skin and irregularly extend to the periphery, seriously affecting the appearance and limb function of the patient. In this study, we found that microRNA-130a (miR-130a) was increased in hypertrophic scar tissues and derived primary f...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zhang J,Zhou Q,Wang H,Huang M,Shi J,Han F,Cai W,Li Y,He T,Hu D

    更新日期:2019-08-15 00:00:00

  • Isolation of 19,20-dehydroprostaglandins E1 and E2 in human seminal fluid and further studies on 18,19-dehydroprostaglandins E1 and E2.

    abstract::Human seminal fluid was recently found to contain 18,19-dehydroprostaglandins E1 and E2 (E. H. Oliw, H. Sprecher, and M. Hamberg, (1986) J. Biol. Chem. 261, 2675-2683). In the present study, the cis and trans isomers of 18,19-dehydroprostaglandins E1 and E2 were prepared by incubation of microsomes of ram vesicular gl...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Oliw EH,Benthin G,Hamberg M

    更新日期:1987-10-01 00:00:00

  • Transcriptional inhibitory role of the tail domains of histone (H3 x H4)2 tetramers.

    abstract::Histone-DNA templates for bacteriophage T7 RNA polymerase were assembled from a plasmid containing a promoter and a terminator for this polymerase, (H3 x H4)2 tetramers deprived of their tail domains, and H2A x H2B dimers. Histone (H3 x H4)2 tetramers lacking their terminal domains were obtained from trypsin-digested ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hernández F,López-Alarcón L,Puerta C,Palacián E

    更新日期:1998-10-01 00:00:00

  • Transcriptional regulation of cellular retinol-binding protein, type II gene expression in small intestine by dietary fat.

    abstract::We have previously demonstrated that dietary fat, especially unsaturated fatty acids, induces cellular retinol-binding protein, type II (CRBPII) gene expression in rat jejunum. In the present study, we showed that feeding a high-fat diet caused parallel increases in jejunal CRBPII mRNA and CRBPII pre-mRNA levels. Nucl...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Suruga K,Mochizuki K,Kitagawa M,Goda T,Horie N,Takeishi K,Takase S

    更新日期:1999-02-01 00:00:00

  • Purification, physicochemical characterization, saccharide specificity, and chemical modification of a Gal/GalNAc specific lectin from the seeds of Trichosanthes dioica.

    abstract::A new galactose-specific lectin has been purified from the extracts of Trichosanthes dioica seeds by affinity chromatography on cross-linked guar gum. The purified lectin (T. dioica seed lectin, TDSL) moved as a single symmetrical peak on gel filtration on Superose-12 in the presence of 0.1 M lactose with an M(r) of 5...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sultan NA,Kenoth R,Swamy MJ

    更新日期:2004-12-15 00:00:00

  • Autophagy enhanced phagocytosis of apoptotic cells by oridonin-treated human histocytic lymphoma U937 cells.

    abstract::Macrophages rapidly engulf and remove apoptotic cells to limit the release of noxious cellular contents and to restrict autoimmune disease or inflammation. Recent developments reveal an important role in autophagy for clearance of apoptotic corpses. However, the relationship between autophagy and phagocytosis remains ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zang L,Xu Q,Ye Y,Li X,Liu Y,Tashiro S,Onodera S,Ikejima T

    更新日期:2012-02-01 00:00:00

  • Purification of a novel isoform of the regulatory subunit of cAMP-dependent protein kinase from the bivalve mollusk Mytilus galloprovincialis.

    abstract::Cytosolic extracts from the posterior adductor muscle of the bivalve mollusk Mytilus galloprovincialis contain significant amounts of both cGMP-binding and cGMP-stimulated protein kinase activities. However, photoaffinity labeling with 8-azido-[32P]cGMP revealed only a major cGMP-binding protein with an apparent molec...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Rodríguez JL,Barcia R,Ramos-Martínez JI,Villamarín JA

    更新日期:1998-11-01 00:00:00

  • Changes in pulmonary calpain activity following treatment of mice with butylated hydroxytoluene.

    abstract::The antioxidant, butylated hydroxytoluene (BHT), causes lung toxicity in mice followed by regenerative repair, and can also modulate the development of carcinogen-induced lung adenomas. We are investigating changes in pulmonary biochemistry following BHT treatment in order to understand the mechanisms of BHT-induced p...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Blumenthal EJ,Malkinson AM

    更新日期:1987-07-01 00:00:00

  • Expression, purification, and in vivo activity of atrial natriuretic factor prohormone produced in Escherichia coli.

    abstract::Atrial muscles of the heart are known to produce polypeptide hormones called atrial natriuretic factors (ANF) which have potent diuretic and hypotensive action. These hormones are synthesized as a larger protein precursor called pro atrial natriuretic factor or proANF which contains the biologically active ANF sequenc...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gierse JK,Olins PO,Devine CS,Marlay JD,Obukowicz MG,Mortensen LH,McMahon EG,Blaine EH,Seetharam R

    更新日期:1989-06-01 00:00:00

  • Structural and kinetic characterization of two 4-oxalocrotonate tautomerases in Methylibium petroleiphilum strain PM1.

    abstract::Methylibium petroleiphilum strain PM1 uses various petroleum products including the fuel additive methyl tert-butyl ether and straight chain and aromatic hydrocarbons as sole carbon and energy sources. It has two operons, dmpI and dmpII, that code for the enzymes in a pair of parallel meta-fission pathways. In order t...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Terrell CR,Burks EA,Whitman CP,Hoffman DW

    更新日期:2013-09-01 00:00:00

  • Structure and assembly of the endoplasmic reticulum: biosynthesis and intracellular sorting of ERp61, ERp59, and ERp49, three protein components of murine endoplasmic reticulum.

    abstract::Rabbit antibodies have been prepared against ERp61, ERp59, and ERp49, three protein components of rough endoplasmic reticulum (RER) purified from mineral oil-induced plasmacytoma 315 (MOPC-315) tissue. Analysis of subcellular fractions of MOPC-315 tissue by an immunoprecipitation procedure demonstrated that all three ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lewis MJ,Mazzarella RA,Green M

    更新日期:1986-03-01 00:00:00