Hypochlorite-modified high-density lipoprotein promotes induction of HO-1 in endothelial cells via activation of p42/44 MAPK and zinc finger transcription factor Egr-1.


:Modification/chlorination of high-density lipoprotein (HDL) by hypochlorous acid (HOCl), formed by the myeloperoxidase-H₂O₂-chloride system of activated phagocytes, converts an anti-atherogenic lipoprotein into a pro-inflammatory lipoprotein particle. Chlorinated HDL is present in human lesion material, binds to and is internalized by endothelial cells and impairs expression and activity of endothelial nitric oxide synthase. The present study aimed at clarifying whether exposure of endothelial cells to pro-inflammatory HOCl-HDL impacts on expression of heme oxygenase-1, a potential rescue pathway against endothelial dysfunction. Our findings revealed that HDL modified by HOCl, added as reagent or generated enzymatically, induced phosphorylation of p42/44 mitogen-activated protein kinase, expression of transcription factor early growth response-1 (Egr-1) and enhanced expression of heme oxygenase-1 in human endothelial cells. Upregulation of heme oxygenase-1 could be blocked by an inhibitor upstream of p42/44 mitogen-activated protein kinase and/or knockdown of Egr-1 by RNA-interference. Electrophoretic mobility shift assays demonstrated HOCl-HDL-mediated induction of the Egr-1 DNA binding activity. Immunocytochemical and immunoblotting experiments demonstrated HOCl-HDL-induced translocation of Egr-1 to the nucleus. The present study demonstrates a novel compensatory pathway against adverse effects of HOCl-HDL, providing cytoprotection in a number of pathological conditions including cardiovascular disease.


Arch Biochem Biophys


Rossmann C,Rauh A,Hammer A,Windischhofer W,Zirkl S,Sattler W,Malle E




Has Abstract


2011-05-01 00:00:00














  • Cancer chemopreventive oltipraz generates superoxide anion radical.

    abstract::The cancer chemopreventive actions of oltipraz, a member of a class of 1,2-dithiolethiones, have been primarily associated with the induction of phase 2 enzymes mediated by a 41bp enhancer element known as the anti-oxidant response element in the promoter regions of many phase 2 genes. It has been suggested that oxyge...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Velayutham M,Villamena FA,Fishbein JC,Zweier JL

    更新日期:2005-03-01 00:00:00

  • Adaptation of protein carbonyl detection to the requirements of proteome analysis demonstrated for hypoxia/reoxygenation in isolated rat liver mitochondria.

    abstract::The key technique in proteome analysis is high-resolution two-dimensional (2D) electrophoretic separation of proteins from biological samples. This method combines isoelectric focusing (IEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Derivatization of protein carbonyls with 2, 4-dinitrop...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Reinheckel T,Körn S,Möhring S,Augustin W,Halangk W,Schild L

    更新日期:2000-04-01 00:00:00

  • Iron-dependent uptake of ascorbate into isolated microsomes.

    abstract::A preliminary study (J.M. Mata, R. Assad, and B. Peterkofsky (1981) Arch. Biochem. Biophys. 206, 93-104) suggested that chick embryo limb bone microsomes took up and concentrated [14C]ascorbate in the presence of cofactors for prolyl hydroxylase. In the present study, we found that the apparent Km for ascorbate in the...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Peterkofsky B,Tschank G,Luedke C

    更新日期:1987-04-01 00:00:00

  • Conformational changes in plastocyanin.

    abstract::The visible and near-uv absorption and circular dichroic spectra were determined for spinach and poplar plastocyanin under a variety of conditions. The visible spectra showed that the copper center was invariant to changes in species, chemical modification with ethylenediamine, and addition of high concentrations of s...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Draheim JE,Anderson GP,Pan RL,Rellick LM,Duane JW,Gross EL

    更新日期:1985-02-15 00:00:00

  • Expression of a plant sesquiterpene cyclase gene in Escherichia coli.

    abstract::5-Epi-aristolochene synthase is a sesquiterpene cyclase activity found in pathogen-challenged tobacco cells, but not in nonchallenged tissues, and appears to be encoded by a complex gene family. As a prerequisite to assessing the functional significance of these multiple genes, bacterial expression systems were examin...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Back K,Yin S,Chappell J

    更新日期:1994-12-01 00:00:00

  • Allosteric effects of cardiac troponin TNT1 mutations on actomyosin binding: a novel pathogenic mechanism for hypertrophic cardiomyopathy.

    abstract::The majority of hypertrophic cardiomyopathy mutations in (cTnT) occur within the alpha-helical tropomyosin binding TNT1 domain. A highly charged region at the C-terminal end of TNT1 unwinds to create a flexible "hinge". While this region has not been structurally resolved, it likely acts as an extended linker between ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Moore RK,Abdullah S,Tardiff JC

    更新日期:2014-06-15 00:00:00

  • Concurrent increase of oxidative DNA damage and lipid peroxidation together with mitochondrial DNA mutation in human lung tissues during aging--smoking enhances oxidative stress on the aged tissues.

    abstract::Although mutation of mitochondrial DNA (mtDNA) in human tissues has been established to associate with intrinsic aging, the impact of environmental factors on the formation and accumulation of mtDNA mutations and oxidative DNA damage in human tissues is poorly understood. We have investigated the levels of mtDNA with ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lee HC,Lim ML,Lu CY,Liu VW,Fahn HJ,Zhang C,Nagley P,Wei YH

    更新日期:1999-02-15 00:00:00

  • Multiple forms of liver microsomal flavin-containing monooxygenases: complete covalent structure of form 2.

    abstract::Hepatic flavin-containing monooxygenases catalyze NADPH-dependent oxygenation of a wide variety of drugs that possess a nucleophilic heteroatom. Two forms of these enzymes (form 1 and 2) have been isolated from rabbit liver microsomes and partially characterized (Ozols, J., 1989, Biochem. Biophys. Res. Commun. 163, 49...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ozols J

    更新日期:1991-10-01 00:00:00

  • Structural investigation of Tet repressor loop 154-167: a time-resolved fluorescence study of three single Trp mutants.

    abstract::We have studied the time-resolved fluorescence of three engineered Tet repressor (TetR) mutants bearing a single Trp residue at positions 162, 163, and 165 in the C-terminal part of the loop joining helices 8 and 9. Detailed analysis indicates that, at 20 degrees, the fluorescence decay of each Trp can be described as...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Alberti P,Bombarda E,Kintrup M,Hillen W,Lami H,Piémont E,Doglia SM,Chabbert M

    更新日期:1997-10-15 00:00:00

  • Ubiquitin C-terminal hydrolase-L5 is required for high glucose-induced transforming growth factor-β receptor I expression and hypertrophy in mesangial cells.

    abstract::Transforming growth factor-β (TGF-β) is pivotal in the pathogenesis of diabetic nephropathy. Type 1 TGF-β receptor (TGF-βR1) is degraded by Smad7-dependent ubiquitination-proteasomal pathway, which is deubiquitinated by ubiquitin C-terminal hydrolase-L5 (UCHL5). Therefore, we studied the role of UCHL5 in high glucose ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ko YM,Chang CY,Chiou SJ,Hsu FJ,Huang JS,Yang YL,Guh JY,Chuang LY

    更新日期:2013-07-15 00:00:00

  • Alteration of pyruvate metabolism in African trypanosomes during differentiation from bloodstream into insect forms.

    abstract::In the presence of glucose and ample oxygen, insect form African trypanosomes release pyruvate more than 100-fold more slowly than do bloodstream forms. This rate decrease could not be accounted for simply by an increased mitochondrial pyruvate oxidation rate as inhibiting mitochondrial respiration increases pyruvate ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Barnard JP,Pedersen PL

    更新日期:1994-08-15 00:00:00

  • The regulatory function of N-terminal AAA+ ATPase domain of eukaryote-like archaeal Orc1/Cdc6 protein during DNA replication initiation.

    abstract::Archaeal replication machinery represents a core version of this in eukaryotes. The crenarchaeon Sulfolobus solfataricus has the potential to be a powerful model system to understand the central mechanism of eukaryotic DNA replication because it contains three active origins of replication and three eukaryote-like Orc...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: He ZG,Feng Y,Wang J,Jiang PX

    更新日期:2008-03-15 00:00:00

  • Inhibitory monoclonal antibodies against rat liver alcohol dehydrogenase.

    abstract::Eleven hybridoma clones which secrete monoclonal antibodies against purified rat liver alcohol dehydrogenase (EC were isolated. Antibodies (R-1-R-11) were identified by their ability to bind to immobilized pure alcohol dehydrogenase in an enzyme-linked immunoadsorbent assay, in which antibody R-9 showed the h...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lad PJ,Schenk DB,Leffert HL

    更新日期:1984-12-01 00:00:00

  • Affinity labeling of eukaryotic initiation factor 2 and elongation factor 1 alpha beta gamma with GTP analogs.

    abstract::As part of an attempt to understand the specific function and role of each subunit in multisubunit protein synthesis factors, we have attempted to identify the nucleotide binding peptides of eukaryotic initiation factor 2 (eIF-2). To ensure that the interactions were of a specific nature, two general controls were use...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Anthony DD Jr,Kinzy TG,Merrick WC

    更新日期:1990-08-15 00:00:00

  • Interaction of glyceraldehyde-3-phosphate dehydrogenase and heme: The relevance of its biological function.

    abstract::GAPDH was speculated to function as a transient trap to reduce the potential toxicity of free heme by a specific and reversible binding with heme. Up to now, there has been lack of studies focused on this effect. In this paper, the efficiency of GAPDH-heme complex on catalyzing protein carbonylation and nitration, the...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Huang Y,Zhang P,Yang Z,Wang P,Li H,Gao Z

    更新日期:2017-04-01 00:00:00

  • Monoterpene biosynthesis: mechanistic evaluation of the geranyl pyrophosphate:(-)-endo-fenchol cyclase from fennel (Foeniculum vulgare).

    abstract::Geranyl pyrophosphate:(-)-endo-fenchol cyclase catalyzes the conversion of geranyl pyrophosphate to (-)-endo-fenchol by a process thought to involve the initial isomerization of the substrate to the tertiary allylic isomer, linalyl pyrophosphate, and the subsequent cyclization of this bound intermediate. Studies with ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Croteau R,Miyazaki JH,Wheeler CJ

    更新日期:1989-03-01 00:00:00

  • Cadmium-induced mRNA expression of Hsp32 is augmented in metallothionein-I and -II knock-out mice.

    abstract::Cadmium is toxic and carcinogenic to humans and animals. The testis and lung are the target organs for cadmium carcinogenesis. Heat shock proteins (HSPs) as well as metallothionein (MT) and glutathione (GSH) play an important role in protection against its toxicity. HSP32, also known as heme oxygenase-1, is a 32-kDa p...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Abe T,Yamamoto O,Gotoh S,Yan Y,Todaka N,Higashi K

    更新日期:2000-10-01 00:00:00

  • The distribution and mechanism of iodotyrosine deiodinase defied expectations.

    abstract::Iodotyrosine deiodinase (IYD) is unusual for its reliance on flavin to promote reductive dehalogenation under aerobic conditions. As implied by the name, this enzyme was first discovered to catalyze iodide elimination from iodotyrosine for recycling iodide during synthesis of tetra- and triiodothyronine collectively k...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Sun Z,Su Q,Rokita SE

    更新日期:2017-10-15 00:00:00

  • Low-molecular-weight thiols in plants: functional and analytical implications.

    abstract::Low-molecular-weight (LMW) thiols are a class of highly reactive compounds massively involved in the maintenance of cellular redox homeostasis. They are implicated in plant responses to almost all stress factors, as well as in the regulation of cellular metabolism. The most studied LMW thiols are glutathione and its b...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Pivato M,Fabrega-Prats M,Masi A

    更新日期:2014-10-15 00:00:00

  • The chalcones cardamonin and flavokawain B inhibit the differentiation of preadipocytes to adipocytes by activating ERK.

    abstract:AIM:We searched for polyphenols capable of inhibiting the lipid accumulation in 3T3-L1 cells, and investigated the mechanisms of two effective chalcones cardamonin and flavokawain B on differentiation of preadipocytes. METHOD AND RESULTS:We treated 3T3-L1 cells with a panel of 46 polyphenols and measured intracellular...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zhang T,Yamamoto N,Yamashita Y,Ashida H

    更新日期:2014-07-15 00:00:00

  • 1H-NMR spectroscopic manifestations of ligand binding to the kringle 4 domain of human plasminogen.

    abstract::Structural aspects of the binding of the linear ligands N alpha-acetyl-L-lysine (AcLys) and epsilon-aminocaproic acid (epsilon ACA) and of the cyclic analogs trans-(aminomethyl)-cyclohexanecarboxylic acid (AMCHA) and p-benzylaminesulfonic acid (BASA) to the intact plasminogen kringle 4 domain have been investigated by...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: De Marco A,Laursen RA,Llinas M

    更新日期:1986-02-01 00:00:00

  • (-)-Epicatechin in the prevention of tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity.

    abstract::An increased permeability of the intestinal barrier is proposed as a major event in the pathophysiology of inflammatory bowel diseases (IBD). Tumor necrosis alpha (TNFα) plays a central role in IBD pathogenesis, in part promoting tight function (TJ) barrier dysfunction. Food extracts enriched in (-)-epicatechin (EC) p...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Contreras TC,Ricciardi E,Cremonini E,Oteiza PI

    更新日期:2015-05-01 00:00:00

  • Influence of the redox state of pyridine nucleotides on mitochondrial sulfhydryl groups and permeability transition.

    abstract::This work addresses a correlation between the redox state of pyridine nucleotides and that of sulfhydryl groups of the mitochondrial membranes. Several major observations emerge: (1) Conditions leading to an oxidation of the pyridine nucleotides such as incubation with tert-butyl hydroperoxide or acetoacetate determin...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Bindoli A,Callegaro MT,Barzon E,Benetti M,Rigobello MP

    更新日期:1997-06-01 00:00:00

  • Characterization of the complete genomic structure of human thromboxane synthase gene and functional analysis of its promoter.

    abstract::Thromboxane A2 (TXA) is a potent vasoconstrictor and mediator of platelet aggregation. Thromboxane synthase (TXAS), which catalyzes the biosynthesis of TXA, is a member of the cytochrome P450 superfamily. We report here the complete genomic structure of the human TXAS gene. The gene contains 13 exons and is 193 kb lon...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Tazawa R,Green ED,Ohashi K,Wu KK,Wang LH

    更新日期:1996-10-15 00:00:00

  • Downregulation of miR-223 and miR-153 mediates mechanical stretch-stimulated proliferation of venous smooth muscle cells via activation of the insulin-like growth factor-1 receptor.

    abstract::Autologous venous grafts, used to circumvent occluded coronary arteries during coronary artery bypass, often develop thrombosis and neointimal hyperplasia. During neointimal hyperplasia, vascular smooth muscle cells (VSMCs), exposed to substantially higher pressure and hemodynamic forces, proliferate and extracellular...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Song L,Duan P,Guo P,Li D,Li S,Xu Y,Zhou Q

    更新日期:2012-12-15 00:00:00

  • Acatalasemia and diabetes mellitus.

    abstract::The enzyme catalase catalyzes the breakdown of hydrogen peroxide into oxygen and water. It is the main regulator of hydrogen peroxide metabolism. Hydrogen peroxide is a highly reactive small molecule formed as a natural byproducts of energy metabolism. Excessive concentrations may cause significant damages to protein,...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Góth L,Nagy T

    更新日期:2012-09-15 00:00:00

  • The kinetics of methotrexate polyglutamation in human breast cancer cells.

    abstract::The polyglutamation kinetics of methotrexate (MTX) in MCF-7 human breast cancer cells have been formulated mathematically. The model takes account of glutamation and hydrolysis kinetics up through the pentaglutamate level, increased synthesis of dihydrofolate reductase following exposure to drug, reversible tight-bind...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Morrison PF,Allegra CJ

    更新日期:1987-05-01 00:00:00

  • Specific electrochemical nitration of horse heart myoglobin.

    abstract::Earlier findings on electronitration of hen egg-white lysozyme demonstrated a product which was mononitrated at Tyr23, by ion-exchange chromatography, absorbance at 430 nm, dithionite reduction, and Edman sequencing of a nitrated proteolytic peptide. However, the whole protein was not sequenced; therefore, although th...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kendall G,Cooper HJ,Heptinstall J,Derrick PJ,Walton DJ,Peterson IR

    更新日期:2001-08-15 00:00:00

  • Glycosphingolipid composition of human semen.

    abstract::Glycosphingolipids were extracted from human semen and purified. Based on the fluorometric assay of sphingosine, in spermatozoa a content of 4.4 +/- 0.9 nmol/10(8) cells of gangliosides and 22.1 +/- 1.7 nmol/10(8) cells of neutral glycosphingolipids was determined. Seminal plasma contained 4.1 +/- 0.6 nmol ganglioside...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ritter G,Krause W,Geyer R,Stirm S,Wiegandt H

    更新日期:1987-09-01 00:00:00

  • Characterization of cyanogenic beta-glucosidase (linamarase) from cassava (Manihot esculenta Crantz).

    abstract::Linamarase (EC was purified from cassava petiole, stem, and root cortex by ammonium sulfate precipitation, column chromatography on Sepharose 6B, and chromatofocusing. The last step resolved the enzyme from each source into three forms with pI values of 4.3, 3.3, and 2.9. Each form was found to be oligomeric...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Eksittikul T,Chulavatnatol M

    更新日期:1988-10-01 00:00:00