Fe-heme structure in Cu, Zn superoxide dismutase from Haemophilus ducreyi by X-ray absorption spectroscopy.

abstract：:Phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, stimulates both the cellular uptake of radiolabeled choline and the activity of CTP:phosphocholine cytidylyltransferase resulting in increased incorporation of choline into phosphatidylcholine (PtdCho). Because of these multiple actions, it...

journal_title：Archives of biochemistry and biophysics

authors： Kiss Z

更新日期：1996-11-01 00:00:00

abstract：:Proliferation and migratory behavior of L929 murine fibroblasts were shown to be modified in the presence of a cytosolic extract of Phormidium sp. (Cyanobacteria). The addition of Phormidium extract to the growth medium (Dulbecco's modified Eagle's medium) supplemented with 0.5% newborn calf serum increased cell proli...

journal_title：Archives of biochemistry and biophysics

authors： Hodara ML,Bré MH,Bouzinba H,Pouphile M,Lefort-Tran M

更新日期：1991-04-01 00:00:00

abstract：:The mechanism by which dietary cholesterol regulates expression of the hepatic low-density lipoprotein (LDL) receptor was investigated. In a previous study (Arch. Biochem. Biophys. 325, 242-248, 1996), we demonstrated that dietary cholesterol reduces the rate of LDL receptor protein degradation without affecting stead...

journal_title：Archives of biochemistry and biophysics

authors： Lopez D,Ness GC

更新日期：1997-08-01 00:00:00

abstract：:The structure of rabbit muscle pyruvate kinase crystallized as a complex with Mg2+, K+, and L-phospholactate (L-P-lactate) has been solved and refined to 2.7 A resolution. The crystals, grown from solutions of polyethylene glycol 8000 at pH 7.5, belong to the space group P2(1) and have unit cell parameters a = 144.4 A...

journal_title：Archives of biochemistry and biophysics

authors： Larsen TM,Benning MM,Wesenberg GE,Rayment I,Reed GH

更新日期：1997-09-15 00:00:00

abstract：:Hyaluronidase from fresh human serum was purified to apparent homogeneity in a two-step procedure. Potent serum inhibitors of hyaluronidase activity were removed during the course of the purification. Isolation of the enzyme was expedited by the use of a newly devised ELISA-like assay. Enzyme activity was measured by ...

journal_title：Archives of biochemistry and biophysics

authors： Afify AM,Stern M,Guntenhöner M,Stern R

更新日期：1993-09-01 00:00:00

abstract：:Advancements in peptide fusion technologies to maximize the protein production has taken a big leap to fulfill the demands of post-genomics era targeting elucidation of structure/function of the proteome and its therapeutic applications, by over-expression in heterologous expression systems. Despite being most preferr...

journal_title：Archives of biochemistry and biophysics

authors： Yadav DK,Yadav N,Yadav S,Haque S,Tuteja N

更新日期：2016-12-15 00:00:00

abstract：:The effect of glutathione (GSH) on the superoxide-sensitive [4Fe-4S]-containing aconitase of Escherichia coli was explored. A mutant deficient in GSH biosynthesis, designated gshA, grew slower in a defined medium than did the parental strain and this effect was more pronounced when succinate was supplied as the carbon...

journal_title：Archives of biochemistry and biophysics

authors： Gardner PR,Fridovich I

更新日期：1993-02-15 00:00:00

abstract：:Uridine kinase is the rate-limiting enzyme in the pyrimidine salvage pathway of all mammalian cells. A cDNA for uridine kinase from mouse brain has been isolated, sequenced, and characterized. This is the first report of a complete nucleotide sequence for mammalian uridine kinase. The isolated cDNA is only 95% complet...

journal_title：Archives of biochemistry and biophysics

authors： Ropp PA,Traut TW

更新日期：1996-12-01 00:00:00

abstract：:Functional concerns regarding hemoglobin-based red cell substitutes have generally centered on two parameters: (a) oxygen binding and delivery properties and (b) stabilization of the hemoglobin tetramer to prevent dimerization. Strategic chemical cross-linking and site-directed mutagenesis have produced proteins that ...

journal_title：Archives of biochemistry and biophysics

authors： Cashon RE,Alayash AI

更新日期：1995-01-10 00:00:00

abstract：:Vitamin D was discovered as an anti-rachitic agent, but even at present, there is no direct evidence to support the concept that vitamin D directly stimulates osteoblastic bone formation and mineralization. It appears to be paradoxical, but vitamin D functions in the process of osteoclastic bone resorption. In 1952, C...

journal_title：Archives of biochemistry and biophysics

authors： Suda T,Takahashi F,Takahashi N

更新日期：2012-07-01 00:00:00

abstract：:Human sorcin, M(r) 22-kDa/pI 5.3, was identified in HOB1 lymphoma cells resistant to 1.0 microM vincristine and polyclonal antibody against it was produced. Using antibody to precipitate sorcin from 32P-labeled cell extract, the SDS-PAGE-resolved human sorcin was not phosphorylated in contrast to the report of A. M. v...

journal_title：Archives of biochemistry and biophysics

authors： Lee WP

更新日期：1996-01-15 00:00:00

abstract：:Studies in vascular smooth muscle cells suggest that, angiotensin II (Ang II)-mediated cellular response requires transactivation of epidermal growth factor receptor (EGF-R), and involves tyrosine phosphorylation of caveolin-1. Here we demonstrate that, exposure of WB rat liver cells to Ang II does not cause transacti...

journal_title：Archives of biochemistry and biophysics

authors： Krishna SB,Alfonso LF,Thekkumkara TJ,Abbruscato TJ,Bhat GJ

更新日期：2007-01-01 00:00:00

abstract：:An anionic peroxidase (EC 1.11.1.7), thought to be involved in suberization, was purified 110-fold from wound-healing slices of Solanum tuberosum by a combination of ammonium sulfate fractionation, Sephadex G-100 gel filtration, isoelectric focusing, and phenyl-Sepharose CL-4B chromatography in 24% yield. The purified...

journal_title：Archives of biochemistry and biophysics

authors： Espelie KE,Kolattukudy PE

更新日期：1985-08-01 00:00:00

abstract：:Recombinant ethanolamine ammonia-lyase from S. typhimurium has been overexpressed and purified in large quantities by a simple procedure. The molecular weight of the native enzyme is about 480 kDa, and it contains two active sites/molecule as shown by kinetic studies and by titration with CNCbl. Analysis by sodium dod...

journal_title：Archives of biochemistry and biophysics

authors： Faust LP,Babior BM

更新日期：1992-04-01 00:00:00

abstract：:The ability of nine phospholipids to alter the activity of low-Km cyclic AMP phosphodiesterase was examined in microsomal fractions of rat adipocytes. The enzyme was activated by phosphatidylserine (21% at 300 microM) and phosphatidylglycerol (36% at 300 microM). The activation was concentration dependent over the ran...

journal_title：Archives of biochemistry and biophysics

authors： Macaulay SL,Kiechle FL,Jarett L

更新日期：1983-08-01 00:00:00

abstract：:The lysosomal cysteine proteinases, cathepsins B, H, and L, were all shown to bind to alpha 2-macroglobulin. The bound enzymes remained active against low-molecular-weight synthetic substrates and bound the active-site-directed inhibitor, benzyloxycarbonyl-125I-Tyr-Ala-diazomethane. Binding of the radiolabeled inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Mason RW

更新日期：1989-09-01 00:00:00

abstract：:Studies were carried out at pH 7.0 and gamma/2 0.15 before addition of CaCl2 or EDTA. Clotting time, tau, at 3.03 microM fibrinogen and 0.91 u/ml thrombin was determined for equilibrium systems. With added Ca2+, tau decreases, from tau 0 at 0 added Ca2+ (mean, 29.7 +/- 3 s), by approximately 3 s at 5 mM added Ca2+. Wi...

journal_title：Archives of biochemistry and biophysics

authors： Perizzolo KE,Sullivan S,Waugh DF

更新日期：1985-03-01 00:00:00

abstract：:Human tumor necrosis factor (TNF), first isolated by our group as an anticancer agent, has been now shown to be a primary mediator of inflammation. Till today 19 different members of the TNF superfamily which interact with 29 different receptors, have been identified. Most members of this family exhibit pro-inflammato...

journal_title：Archives of biochemistry and biophysics

authors： Gupta SC,Tyagi AK,Deshmukh-Taskar P,Hinojosa M,Prasad S,Aggarwal BB

更新日期：2014-10-01 00:00:00

abstract：:A kinetic quantification of the lipoperoxyl radical-scavenging activity of all-trans-retinol has been carried out in homogeneous solution, when radicals were produced from the oxidation of methyl linoleate in methanol, initiated by the lipid-soluble 2,2'-azobis (2,4-dimethylvaleronitrile) (AMVN) as well as in a soybea...

journal_title：Archives of biochemistry and biophysics

authors： Tesoriere L,Ciaccio M,Bongiorno A,Riccio A,Pintaudi AM,Livrea MA

更新日期：1993-11-15 00:00:00

abstract：:We have studied the influence of D-amino acid substitution in the flanking region on the antibody recognition of the 19TGTQ22 epitope core in the tandem repeat of mucin 2 (MUC2) glycoprotein. Analogue peptides corresponding to the optimal epitope sequence (16PTPTGTQ22) have been prepared by the replacement of single o...

journal_title：Archives of biochemistry and biophysics

authors： Uray K,Kajtár J,Vass E,Price MR,Hollósi M,Hudecz F

更新日期：2000-06-01 00:00:00

abstract：:Previous studies have shown that reactive oxygen species (ROS) enhance arachidonic acid (AA) release and the subsequent AA metabolism in macrophages. The purpose of this study was determined the implication of phospholipases A2 (PLA2s) in these events. Our results show that oxidative stress induced by exogenous adding...

journal_title：Archives of biochemistry and biophysics

authors： Martínez J,Moreno JJ

更新日期：2001-08-15 00:00:00

abstract：:Lubrol-solubilized rabbit thrombomodulin has been examined by equilibrium sedimentation in buffers that include sufficient D2O to make the detergent neutrally buoyant. Data were acquired at rotor speeds from 12,000 to 28,000 rpm from two thrombomodulin preparations, at protein concentrations from 0.01 to 0.07%, and in...

journal_title：Archives of biochemistry and biophysics

authors： Winnard PT,Esmon CT,Laue TM

更新日期：1989-02-15 00:00:00

abstract：:This work addresses a correlation between the redox state of pyridine nucleotides and that of sulfhydryl groups of the mitochondrial membranes. Several major observations emerge: (1) Conditions leading to an oxidation of the pyridine nucleotides such as incubation with tert-butyl hydroperoxide or acetoacetate determin...

journal_title：Archives of biochemistry and biophysics

authors： Bindoli A,Callegaro MT,Barzon E,Benetti M,Rigobello MP

更新日期：1997-06-01 00:00:00

abstract：:Binding of ligands to the catalytic center of mammalian triosephosphate isomerase (TPI) induces a conformational change(s) that enhances the specific deamidation of Asn71 at the subunit interface. Deamidation initiates dissociation and degradation of the enzyme in vivo and in vitro. We have utilized circular dichroism...

journal_title：Archives of biochemistry and biophysics

authors： Sun AQ,Yüksel KU,Rao GS,Gracy RW

更新日期：1992-06-01 00:00:00

abstract：:MICALs form a conserved multidomain protein family essential for cytoskeletal rearrangements. To complement structural information available, we produced the FAD-containing monooxygenase-like domain of human MICAL-1 (MICAL-MO) in forms differing for the presence and location of a His-tag, which only influences the pro...

journal_title：Archives of biochemistry and biophysics

authors： Zucchini D,Caprini G,Pasterkamp RJ,Tedeschi G,Vanoni MA

更新日期：2011-11-01 00:00:00

abstract：:A soluble monoterpene primary alcohol:NADP+ oxidoreductase has been purified to apparent homogeneity from leaves of the catmint, Nepeta racemosa. The purified enzyme consisted of two polypeptides, with molecular masses of 42,000 and 40,000 Da, and contained zinc ions. A number of monoterpene alcohols (geraniol, nerol,...

journal_title：Archives of biochemistry and biophysics

authors： Hallahan DL,West JM,Wallsgrove RM,Smiley DW,Dawson GW,Pickett JA,Hamilton JG

更新日期：1995-04-01 00:00:00

abstract：:We have previously shown that exposure of Clone 9 cells to hypoxia, cyanide, or azide results in an acute stimulation of glucose transport that is largely mediated by "activation" of glucose transporter (Glut1) sites preexisting in the plasma membrane. However, it is not known whether inhibition of oxidative phosphory...

journal_title：Archives of biochemistry and biophysics

authors： Hamrahian AH,Zhang JZ,Elkhairi FS,Prasad R,Ismail-Beigi F

更新日期：1999-08-15 00:00:00

abstract：:Studies in eucaryotic cells (mainly animals and yeast) indicate that at least two DNA polymerases are involved in DNA replication at the level of the replication fork: DNA polymerase alpha, which is associated with DNA primase, is involved in the replication of the lagging strand; DNA polymerase delta, associated with...

journal_title：Archives of biochemistry and biophysics

authors： Richard MC,Litvak S,Castroviejo M

更新日期：1991-05-15 00:00:00

abstract：:Sequence analyses of the C-terminal membrane intercalative region of the rat cytochrome b(5) indicated that this domain has, in addition to a signal sequence, a combined element of the classic stop-transfer sequence typically found in a variety of transmembrane proteins. Such bitopic protein arrangements arise by tand...

journal_title：Archives of biochemistry and biophysics

authors： Kaderbhai MA,Morgan R,Kaderbhai NN

更新日期：2003-04-15 00:00:00

abstract：:Chromatium vinosum contains a polypeptide that is functionally and structurally similar to the Escherichia coli chaperonin 10. The protein has been purified to homogeneity by sucrose density gradient centrifugation followed by gel filtration using a Bio-Gel A-1.5 m column. The molecular mass of chaperonin 10, as deter...

journal_title：Archives of biochemistry and biophysics

authors： Torres-Ruiz JA,McFadden BA

更新日期：1992-05-15 00:00:00