Reaction of human hemoglobin HbA0 and two cross-linked derivatives with hydrogen peroxide: differential behavior of the ferryl intermediate.

abstract：:Reactive oxygen species like hydrogen peroxide (H2O2) have been shown to serve as messengers in the induction of NF-kappa B and, hence, in the activation and replication of human immunodeficiency virus type 1 (HIV-1) in human cells. Several antioxidant compounds and iron chelators have been shown to interfere with bot...

journal_title：Archives of biochemistry and biophysics

authors： Sappey C,Boelaert JR,Legrand-Poels S,Grady RW,Piette J

更新日期：1995-08-01 00:00:00

abstract：:The decomposition of S-nitrosoglutathione (GSNO) in the presence of Cu2+ and glutathione (GSH) was studied by stopped-flow/rapid-scan spectroscopy. Reduction of Cu2+ by GSH and subsequent formation of a GS-*Cu+ complex occurred within 200 ms, with the amount of complex formed depending on the GSH-to-Cu2+ ratio. The ra...

journal_title：Archives of biochemistry and biophysics

authors： Gorren AC,Schrammel Astrid,Schmidt K,Mayer B

更新日期：1996-06-15 00:00:00

abstract：:The effects of insulin on phospholipid metabolism and generation of diacylglycerol (DAG) and on activation of protein kinase C in rat hepatocytes were compared to those of vasopressin and angiotension II. Insulin provoked increases in [3H]glycerol labeling of phosphatidic acid (PA), diacylglycerol (DAG), and other gly...

journal_title：Archives of biochemistry and biophysics

authors： Cooper DR,Hernandez H,Kuo JY,Farese RV

更新日期：1990-02-01 00:00:00

abstract：:Biotin sulfoxide reductase catalyzes the conversion of d-biotin d-sulfoxide (BSO) to d-biotin. Oligonucleotides directed against common sequences in Escherichia coli biotin sulfoxide reductase and in Rhodobacter sphaeroides f.sp. denitrificans dimethyl sulfoxide reductase have been utilized to amplify by PCR a 651-bp ...

journal_title：Archives of biochemistry and biophysics

authors： Pollock VV,Barber MJ

更新日期：1995-04-20 00:00:00

abstract：:Activation of the delta-isoform of protein kinase C (deltaPKC) by certain conditions of oxidative stress results in translocation of the kinase to the mitochondria leading to release of cytochrome c and the induction of apoptosis. In the current study, the effects of myocardial reperfusion-induced deltaPKC translocati...

journal_title：Archives of biochemistry and biophysics

authors： Churchill EN,Szweda LI

更新日期：2005-07-15 00:00:00

abstract：:The excimer fluorescence of pyrene iodoacetamide-labeled tropomyosin bound to actin is a probe of the regulatory state change of the muscle thin filament associated with the cooperative binding of myosin subfragment 1 (S1) (Y. Ishii and S. S. Lehrer, Biochemistry 29, 1160, 1990). In this study, the excimer fluorescenc...

journal_title：Archives of biochemistry and biophysics

authors： Ishii Y,Lehrer SS

更新日期：1993-08-15 00:00:00

abstract：:Functional activities of isolated atria from 4-week diabetic rats and their anoxic tolerance and recovery were compared to those of control rats. The present study showed a two-fold increase in maximum developed contraction force (CF), the rate of force development, and the rate of relaxation in isolated atria from di...

journal_title：Archives of biochemistry and biophysics

authors： Savabi F,Kirsch A

更新日期：1990-05-15 00:00:00

abstract：:The mechanism of the biosynthesis of 12-oxo-10,15(Z)-phytodienoic acid (12-oxo-PDA) from 13(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid in preparations of corn (Zea mays L.) was studied. In the initial reaction the hydroperoxide was converted into an unstable allene oxide, 12,13(S)-epoxy-9(Z),11,15(Z)-octade...

journal_title：Archives of biochemistry and biophysics

authors： Hamberg M,Fahlstadius P

更新日期：1990-02-01 00:00:00

abstract：:The digestion of tubulin with subtilisin and the reassociation of the digestion products was followed by means of the fluorescent probe 4',6-diamidino-2-phenylindole (DAPI). The fluorescence spectra of DAPI bound to chicken brain tubulin and to the main products of tubulin digested with subtilisin-agarose (tubulin S a...

journal_title：Archives of biochemistry and biophysics

authors： Ortiz M,Lagos R,Monasterio O

更新日期：1993-05-15 00:00:00

abstract：:The first evidence for the reductive metabolism of a noncyclic nitrosamine to the corresponding hydrazine derivative in vivo and in vitro is provided. Under anaerobic conditions, N-nitrosodiphenylamine was reduced by guinea pig liver 9000g supernatant to 1,1-diphenylhydrazine in the presence of 2-hydroxypyrimidine or ...

journal_title：Archives of biochemistry and biophysics

authors： Tatsumi K,Yamada H,Kitamura S

更新日期：1983-10-01 00:00:00

abstract：:The motility of actin filaments interacting with heavy meromyosin molecules was directly observed on indium tin oxide-coated glass (ITO-glass), over which a surface current flowed. Because the increase in surface current applied to ITO-glass increases the temperature, we focused on the temperature-dependence of the sl...

journal_title：Archives of biochemistry and biophysics

authors： Wada R,Sato D,Nakamura T,Hatori K

更新日期：2015-11-15 00:00:00

abstract：:Diethyl dithiocarbamate (DDC) was used to inhibit the copper- and zinc- containing superoxide dismutase (Cu,ZnSOD) of Escherichia coli in order to expose its physiological function. DDC inhibited the aerobic growth of a sodA sodB mutant much more than it did the growth of a SOD-replete parental strain and this inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Benov L,Fridovich I

更新日期：1996-03-15 00:00:00

abstract：:The rate constant for quenching, kq, of the phosphorescence of 6-bromo-2-naphthyl sulfate (BNS) by cytochromes c, cytochrome c peroxidase, catalase, and myoglobin has been measured as a function of temperature and solvent viscosity. In aqueous solution at pH 7.0 for cytochromes c and myoglobin the value of kq is nearl...

journal_title：Archives of biochemistry and biophysics

authors： Beckham S,Cook MP,Karki L,Luchsinger MM,Whitlock VR,Wu Y,Zhang Q,Schuh MD

更新日期：1994-05-01 00:00:00

abstract：:Experimental and computational studies suggest that few general principles govern protein/protein interactions and aggregation. The knowledge of these rules may be exploited to design peptides that are able to interfere with the self-assembly and aggregation of proteins. This work is aimed to verify the validity of th...

journal_title：Archives of biochemistry and biophysics

authors： La Rosa C,Milardi D,Amato E,Pappalardo M,Grasso D

更新日期：2005-03-01 00:00:00

abstract：:Radiotherapy plays a limited role in the treatment of hepatocellular carcinoma (HCC) due to the development of resistance. Therefore, further investigation of underlying mechanisms involved in HCC radioresistance is warranted. Increasing evidence shows that long non-coding RNAs (linc-RNAs) are involved in the patholog...

journal_title：Archives of biochemistry and biophysics

authors： Chen Y,Shen Z,Zhi Y,Zhou H,Zhang K,Wang T,Feng B,Chen Y,Song H,Wang R,Chu X

更新日期：2018-05-01 00:00:00

abstract：:An N-acetyl-D-galactosamine-specific lectin has been isolated from the two seed forms of the hog peanut (Amphicarpaea bracteata) using an affinity support containing the synthetic type A blood group trisaccharide alpha-D-GalNAc-(1,3)-[alpha-L-Fuc-(1,2)]-beta-D-Gal (Synsorb A). The affinity-purified lectin appears to b...

journal_title：Archives of biochemistry and biophysics

authors： Maliarik MJ,Roberts DD,Goldstein IJ

更新日期：1987-05-15 00:00:00

abstract：:Glucosylsphingosine is a potent inhibitor of lysosomal glucocerebrosidase and the broad-specificity, cytosolic beta-glucosidase of human liver. In the present study, it was demonstrated that the broad-specificity beta-glucosidase was also inhibited by galactosylsphingosine. The inhibition was observed when the enzyme ...

journal_title：Archives of biochemistry and biophysics

authors： LaMarco KL,Glew RH

更新日期：1985-02-01 00:00:00

abstract：:After an energy source shift-down, Escherichia coli accumulates 70 S ribosome-mRNA complexes ("70 S monosomes"). The monosome mRNA strands are predominantly primary transcription products with purine nucleoside 5'-triphosphate and 5'-diphosphate termini present at a 1:2 ratio. The number-average chain length is 564 +/...

journal_title：Archives of biochemistry and biophysics

authors： Wnek AP,Jacobson LA

更新日期：1985-08-15 00:00:00

abstract：:A cDNA that encodes the human plasminogen (HPg) amino acid sequence has been inserted adjacent to the polyhedrin promoter in the genome of the baculovirus, Autographa californica nuclear polyhedrosis virus, which was then used to infect cultured cells of the farm armyworm, Spodoptera frugiperda. Under the conditions o...

journal_title：Archives of biochemistry and biophysics

authors： Whitefleet-Smith J,Rosen E,McLinden J,Ploplis VA,Fraser MJ,Tomlinson JE,McLean JW,Castellino FJ

更新日期：1989-06-01 00:00:00

abstract：:Isolated adrenocortical carcinoma cells of rat contain alpha 2- and beta-adrenergic receptors. When these cells are incubated with alpha 2-adrenergic agonists, there is a concentration-dependent increase of cyclic GMP that is blocked by the alpha 2-adrenergic antagonist yohimbine but not by the beta-antagonist propran...

journal_title：Archives of biochemistry and biophysics

authors： Jaiswal N,Sharma RK

更新日期：1986-09-01 00:00:00

abstract：:Inactivation of the von Hippel-Lindau (VHL) tumor suppressor is associated with renal carcinoma, hemangioblastoma and pheochromocytoma. The VHL protein is a component of a ubiquitin ligase complex that ubiquitinates and degrades hypoxia inducible factor-α (HIF-α). Degradation of HIF-α by VHL is proposed to suppress tu...

journal_title：Archives of biochemistry and biophysics

authors： Lai Y,Song M,Hakala K,Weintraub ST,Shiio Y

更新日期：2012-02-15 00:00:00

abstract：:A theoretical and experimental study has been made of the advancing elution profile in frontal gel chromatography of interacting systems for which the elution volume of the complex is smaller than that of the larger reactant. First, Gilbert-Jenkins theory is used to delineate the form of the elution profile from the m...

journal_title：Archives of biochemistry and biophysics

authors： Cann JR,Winzor DJ

更新日期：1987-07-01 00:00:00

abstract：:Mitochondrial carbonic anhydrase has previously been quantitated in liver mitochondria; it was not detected in guinea pig kidney cortical mitochondria. Evidence of this enzyme in rat kidney cortical mitochondria is reported. Electron microscopy showed that intact mitochondria were free of other intracellular organelle...

journal_title：Archives of biochemistry and biophysics

authors： Dodgson SJ,Contino LC

更新日期：1988-01-01 00:00:00

abstract：:Rodobacter capsulatus cells, which were cultured anaerobically in high light intensity, had fewer foldings in the cytoplasmic membrane than those which were grown in lower light intensities. Spheroplast-derived membrane fractions obtained from cells cultured under high light intensity contained a high yield of large r...

journal_title：Archives of biochemistry and biophysics

authors： Carmeli C,Lifshitz Y,Friedberg I

更新日期：1991-08-01 00:00:00

abstract：:We describe herein the design and in vitro biochemical evaluation of a novel class of mechanism-based inhibitors of human leukocyte elastase (HLE) that inactivate the enzyme via an unprecedented enzyme-induced sulfonamide fragmentation cascade. The inhibitors incorporate in their structure an appropriately functionali...

journal_title：Archives of biochemistry and biophysics

authors： Wei L,Lai Z,Gan X,Alliston KR,Zhong J,Epp JB,Tu J,Perera AB,Van Stipdonk M,Groutas WC

更新日期：2004-09-01 00:00:00

abstract：:Factors influencing pyrroline 5-carboxylate (P5C) synthesis from glutamate by a subcellular fraction enriched in mitochondria of rat small intestinal mucosa have been studied. P5C synthesis decreased rapidly if this subcellular fraction was preincubated at 20 degrees C in the absence of substrates; this effect suggest...

journal_title：Archives of biochemistry and biophysics

authors： Henslee JG,Wakabayashi Y,Small C,Jones ME

更新日期：1983-10-15 00:00:00

abstract：:The radioactive, photoactivatable labeling probe [beta-32P]5-azidouridine 5'-diphosphoglucose has recently been shown to label a 62-kDa protein in crude homogenates and in partially purified enzyme preparations without photoactivation. Here, we report that a portion of this radioactivity is due to labeling of phosphog...

journal_title：Archives of biochemistry and biophysics

authors： Marchase RB,Richardson KL,Srisomsap C,Drake RR,Haley BE

更新日期：1990-07-01 00:00:00

abstract：:Earlier findings on electronitration of hen egg-white lysozyme demonstrated a product which was mononitrated at Tyr23, by ion-exchange chromatography, absorbance at 430 nm, dithionite reduction, and Edman sequencing of a nitrated proteolytic peptide. However, the whole protein was not sequenced; therefore, although th...

journal_title：Archives of biochemistry and biophysics

authors： Kendall G,Cooper HJ,Heptinstall J,Derrick PJ,Walton DJ,Peterson IR

更新日期：2001-08-15 00:00:00

abstract：:Phorbol esters induce rapid downregulation of PAF receptors on human neutrophils. The molecular mechanism responsible for this observation has not been elucidated. In the present study, treatment of neutrophils with PMA (10(-7) M) for 10 min caused a complete loss of PAF receptors from the cell surface as determined b...

journal_title：Archives of biochemistry and biophysics

authors： Zhou W,Olson MS

更新日期：1994-08-15 00:00:00

abstract：:Bovine kidney mitochondrial extracts contain an inactive protamine kinase and an inactive casein kinase. The protamine kinase was activated by chromatography on poly(L-lysine)-agarose. Two forms of this soluble mitochondrial protamine kinase were separated by chromatography on protamine-agarose. Both forms were purifi...

journal_title：Archives of biochemistry and biophysics

authors： Damuni Z,Reed LJ

更新日期：1988-05-01 00:00:00