Abstract：
:Experimental and computational studies suggest that few general principles govern protein/protein interactions and aggregation. The knowledge of these rules may be exploited to design peptides that are able to interfere with the self-assembly and aggregation of proteins. This work is aimed to verify the validity of this hypothesis by investigating the interaction of cytochrome c with Phe and Gly amino acids, Ala-His (carnosine), and two water-soluble dipeptides Phe-Gly and Gly-Phe. The combined use of (1)H NMR, MD, and DSC has shown that: (i) at neutral pH, only Phe-Gly is able to prevent the thermally induced aggregation of cytochrome c; (ii) Phe-Gly interacts with Gly45 and Phe46 residues of the protein, either when the protein is in the folded or in the unfolded state; and (iii) the interaction of Phe-Gly with cytochrome c is sequence-dependent. These results support the hypothesis that the basic principles that describe protein aggregation can be used for the design of peptides with antiaggregating properties.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
La Rosa C,Milardi D,Amato E,Pappalardo M,Grasso Ddoi
10.1016/j.abb.2004.12.006subject
Has Abstractpub_date
2005-03-01 00:00:00pages
182-9issue
1eissn
0003-9861issn
1096-0384pii
S0003-9861(04)00697-6journal_volume
435pub_type
杂志文章abstract：:Because angiogenesis underlies the pathogenesis of numerous conditions (cancer, psoriasis, macular degeneration), there is a pressing need for continued investigations into angiogenic signaling and potential drug targets. Antiangiogenic agents can be classified as either direct or indirect. Direct antiangiogenics act ...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章,评审
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更新日期：2011-04-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1996.0537
更新日期：1996-12-01 00:00:00
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doi：10.1016/0003-9861(91)90020-j
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(91)90192-l
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pub_type： 杂志文章
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更新日期：2013-09-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.2000.2111
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/j.abb.2007.09.022
更新日期：2007-12-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1995.9962
更新日期：1995-11-10 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/s0003-9861(02)00218-7
更新日期：2002-07-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(86)90504-7
更新日期：1986-08-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(86)90192-x
更新日期：1986-02-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.abb.2014.11.012
更新日期：2015-04-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(92)90578-k
更新日期：1992-07-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/j.abb.2012.08.001
更新日期：2012-12-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1996.0454
更新日期：1996-10-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
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更新日期：1988-10-01 00:00:00
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doi：10.1016/0003-9861(90)90608-2
更新日期：1990-11-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
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更新日期：2006-04-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
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更新日期：2013-12-01 00:00:00
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更新日期：1989-05-15 00:00:00
abstract：:Cytosolic proteins may play an important role in the intracellular transport of bile acids in enterocytes. The lithocholate binding properties of cytosolic protein from bovine small intestine were studied. Lithocholate binding was observed in the Y (45-50 kDa), Y' (30-35 kDa), and Z fractions (10-15 kDa) following gel...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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更新日期：1992-11-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1999.1594
更新日期：2000-02-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
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更新日期：1994-12-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/j.abb.2013.08.013
更新日期：2014-06-15 00:00:00
abstract：:Bacillus stearothermophilus was grown at the optimal temperature range (center, 65 degrees C), below it (48 and 55 degrees C), and above it (68 degrees C), in a complex medium with or without 2.5 mM Ca2+. The Ca(2+)-supplement improves growth at sub- and supraoptimal temperatures and extends it to higher temperatures ...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(91)90457-t
更新日期：1991-08-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1994.1406
更新日期：1994-10-01 00:00:00
abstract：:Sarcolemmal vesicles of white and red skeletal muscles of the rabbit were prepared by consecutive density gradient centrifugations in sucrose and dextran according to Seiler and Fleischer (1982, J. Biol. Chem. 257, 13,862-13,871). White and red muscle membrane fractions enriched in sarcolemma were characterized by hig...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(90)90501-o
更新日期：1990-06-01 00:00:00
