当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Rat kidney mitochondrial carbonic anhydrase.

Rat kidney mitochondrial carbonic anhydrase.

Abstract：

:Mitochondrial carbonic anhydrase has previously been quantitated in liver mitochondria; it was not detected in guinea pig kidney cortical mitochondria. Evidence of this enzyme in rat kidney cortical mitochondria is reported. Electron microscopy showed that intact mitochondria were free of other intracellular organelles. When intact kidney mitochondria were added to isotonic 3'-(N'-morpholino) propanesulfonic acid buffer with 25 mM KHCO3 (1% labeled with 18O) the rate of disappearance of C18O16O was biphasic; this indicates that there is carbonic anhydrase within the inner mitochondrial membrane. Intact rat kidney mitochondria were assayed for carbonic anhydrase activity at 4 degrees C by the changing pH technique. The rate of CO2 hydration in the presence and absence of intact mitochondria was identical; this rate increased when Triton X-100 was added which indicates that all carbonic anhydrase is inside the inner mitochondrial membrane. Carbonic anhydrase activity was quantitated as kenz (units, ml.s-1 mg-1 mitochondrial protein) at 37 degrees C, pH 7.4, in 25 mM NaHCO3 (1% labeled with 18O) by following the rate of disappearance of C18O16O from solutions before and after addition of disrupted mitochondria. Values of Kenz for liver and kidney mitochondria from rats given free access to normal rat chow and water at neutral pH were 0.06 and 0.08 (respectively). Values of kenz for liver and kidney mitochondria from rats fed as above and with free access to water adjusted to pH 2.5 with HCl were 0.04 and 0.16, respectively. Values of kenz for rats starved for 48 h were 0.06 and 0.12 (respectively). The values of kenz remained 0.11-0.14 in liver mitochondria from guinea pigs fed normally, given dilute acid, or starved and the value was always at zero in guinea pig kidney mitochondria. Values of Kenz were measured with disrupted mitochondria by the 18O technique as a function of pH at 25 degrees C, 25 to 75 mM NaHCO3, ionic strength 0.3. From pH 7.0 to 8.0 kenz increased threefold for mitochondria from rat liver, fed rat kidney, and acid rat kidney, and increased eightfold for mitochondria from guinea pig liver. kenz was decreased similarly by increasing HCO3- in mitochondria from rat liver, fed kidney, and acid kidney; it is concluded that carbonic anhydrase in rat liver mitochondria is probably the same isozyme as in rat kidney mitochondria. The published observation that rat kidney cortices are up to 10 times as gluconeogenic from pyruvate as guinea pig kidney cortices can be explained by the presence of mitochondrial carbonic anhydrase in rat but not guinea pig mitochondria.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Dodgson SJ,Contino LC

doi

10.1016/0003-9861(88)90457-2

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

334-41

issue

1

eissn

0003-9861

issn

1096-0384

pii

0003-9861(88)90457-2

journal_volume

260

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Equilibrium titrations of acid-induced unfolding-refolding and salt-induced molten globule of cytochrome c by FT-IR spectroscopy.

    abstract：:Despite extensive investigations on the acid-unfolded and acid/salt-induced molten globule(-like) states of cytochrome c using variety of techniques, structural features of the acid-unfolded state in terms of residual secondary structures and the structural transition between the acid-unfolded and acid/salt-refolded s...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2005.01.006

    authors： Dong A,Lam T

    更新日期：2005-04-01 00:00:00

  • Stimulation by heme of steryl ester synthase and aerobic sterol exclusion in the yeast Saccharomyces cerevisiae.

    abstract：:Saccharomyces cerevisiae sterol and heme auxotrophs were used to elucidate a role for hemes in sterol esterification. Steryl ester synthase (SES) activity was stimulated on average fourfold in cells supplemented with 50 micrograms/ml delta-aminolevulinic acid (ALA). This stimulation was not dependent on ALA per se, bu...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90600-2

    authors： Keesler GA,Casey WM,Parks LW

    更新日期：1992-08-01 00:00:00

  • Cytochrome c: a catalyst and target of nitrite-hydrogen peroxide-dependent protein nitration.

    abstract：:Nitration of protein tyrosine residues to 3-nitrotyrosine (NO2Tyr) serves as both a marker and mediator of pathogenic reactions of nitric oxide (*NO), with peroxynitrite (ONOO-) and leukocyte peroxidase-derived nitrogen dioxide (*NO2) being proximal mediators of nitration reactions in vivo. Cytochrome c is a respirato...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2003.08.033

    authors： Castro L,Eiserich JP,Sweeney S,Radi R,Freeman BA

    更新日期：2004-01-01 00:00:00

  • The missing links within troponin.

    abstract：:The cardiac contraction-relaxation cycle is controlled by a sophisticated set of machinery. Of particular interest, is the revelation that allosteric networks transmit effects of binding at one site to influence troponin complex dynamics and structural-mediated signaling in often distal, functional sites in the myofil...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2018.12.026

    authors： Marques MA,Parvatiyar MS,Yang W,de Oliveira GAP,Pinto JR

    更新日期：2019-03-15 00:00:00

  • Investigation of plasma induced electrical and chemical factors and their contribution processes to plasma gene transfection.

    abstract：:This study has been done to know what kind of factors in plasmas and processes on cells induce plasma gene transfection. We evaluated the contribution weight of three groups of the effects and processes, i.e. electrical, chemical and biochemical ones, inducing gene transfection. First, the laser produced plasma (LPP) ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2016.04.013

    authors： Jinno M,Ikeda Y,Motomura H,Kido Y,Satoh S

    更新日期：2016-09-01 00:00:00

  • How iron is handled in the course of heme catabolism: Integration of heme oxygenase with intracellular iron transport mechanisms mediated by poly (rC)-binding protein-2.

    abstract：:Heme and iron are essential to almost all forms of life. The strict maintenance of heme and iron homeostasis is essential to prevent cellular toxicity and the existence of systemic and intracellular regulation is fundamental. Cytosolic heme can be catabolized and detoxified by heme oxygenases (HOs). Interestingly, fre...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2019.108071

    authors： Yanatori I,Richardson DR,Toyokuni S,Kishi F

    更新日期：2019-09-15 00:00:00

  • Monoterpene biosynthesis: mechanistic evaluation of the geranyl pyrophosphate:(-)-endo-fenchol cyclase from fennel (Foeniculum vulgare).

    abstract：:Geranyl pyrophosphate:(-)-endo-fenchol cyclase catalyzes the conversion of geranyl pyrophosphate to (-)-endo-fenchol by a process thought to involve the initial isomerization of the substrate to the tertiary allylic isomer, linalyl pyrophosphate, and the subsequent cyclization of this bound intermediate. Studies with ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90134-3

    authors： Croteau R,Miyazaki JH,Wheeler CJ

    更新日期：1989-03-01 00:00:00

  • Evaluation of the conformational equilibrium of reduced hen egg lysozyme by antibodies to the native form.

    abstract：:To evaluate the conformation of reduced HEL, the monoclonal antibodies HyC1 and HyC2, which recognize different conformational epitopes on native hen egg lysozyme (HEL), were used, and the kinetics of their interactions with native HEL, S-1,2-dicarboxyethylated HEL (DCE-HEL), and carboxymethylated Cys6 and Cys127 HEL ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2009.11.024

    authors： Oda M,Kitai A,Murakami A,Nishimura M,Ohkuri T,Abe Y,Ueda T,Nakamura H,Azuma T

    更新日期：2010-02-15 00:00:00

  • Tryparedoxin peroxidase of Leishmania donovani: molecular cloning, heterologous expression, specificity, and catalytic mechanism.

    abstract：:Tryparedoxin peroxidase (TXNPx) of Trypanosomatidae is the terminal peroxidase of a complex redox cascade that detoxifies hydroperoxides by NADPH (Nogoceke et al., Biol. Chem. 378, 827-836, 1997). A gene putatively coding for a peroxiredoxin-type TXNPx was identified in L. donovani and expressed in Escherichia coli to...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2001.2688

    authors： Flohé L,Budde H,Bruns K,Castro H,Clos J,Hofmann B,Kansal-Kalavar S,Krumme D,Menge U,Plank-Schumacher K,Sztajer H,Wissing J,Wylegalla C,Hecht HJ

    更新日期：2002-01-15 00:00:00

  • Abnormal membrane properties of the sarcoplasmic reticulum of pigs susceptible to malignant hyperthermia: modes of action of halothane, caffeine, dantrolene, and two other drugs.

    abstract：:The role of sarcoplasmic reticulum (SR) in malignant hyperthermia (MH) was studied using the heavy microsomal fraction prepared from semitendinosus muscles of both normal and genetically MH-susceptible pigs. In the presence of ATP, SR was loaded with 70 nmol Ca2+/mg SR protein. Under these conditions, MH-SR demonstrat...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(86)90587-4

    authors： Ohnishi ST,Waring AJ,Fang SR,Horiuchi K,Flick JL,Sadanaga KK,Ohnishi T

    更新日期：1986-06-01 00:00:00

  • The oxidation of ebselen metabolites to thiol oxidants catalyzed by liver microsomes and perfused rat liver.

    abstract：:The oxidation of 2-(methylseleno)benzanilide and 2-selenylbenzanilide, metabolites of the antioxidant drug ebselen, was examined in reactions catalyzed by rat, pig, and guinea pig liver microsomes and in perfused rat liver. Microsomes from all three species catalyzed NADPH- and oxygen-dependent oxidation of the seleni...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1031

    authors： Akerboom TP,Sies H,Ziegler DM

    更新日期：1995-01-10 00:00:00

  • Rational design of tropoelastin peptide-based inhibitors of metalloproteinases.

    abstract：:Abnormal production of matrix metalloproteinases (MMPs) has been observed in a variety of diseases, such as emphysema, atherosclerosis, and cancer metastasis. Destruction of connective tissue ensues and elastin is often a key target. Three of the main elastolytic MMPs are the gelatinases MMP-2 and MMP-9 and the metall...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/s0003-9861(02)00612-4

    authors： Jensen SA,Andersen P,Vrhovski B,Weiss AS

    更新日期：2003-01-15 00:00:00

  • Increased myocardial short-range forces in a rodent model of diabetes reflect elevated content of β myosin heavy chain.

    abstract：:Diastolic dysfunction is a clinically significant problem for patients with diabetes and often reflects increased ventricular stiffness. Attached cross-bridges contribute to myocardial stiffness and produce short-range forces, but it is not yet known whether these forces are altered in diabetes. In this study, we test...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2013.08.013

    authors： Chung CS,Mitov MI,Callahan LA,Campbell KS

    更新日期：2014-06-15 00:00:00

  • Effects of calcium binding and of EDTA and CaEDTA on the clotting of bovine fibrinogen by thrombin.

    abstract：:Studies were carried out at pH 7.0 and gamma/2 0.15 before addition of CaCl2 or EDTA. Clotting time, tau, at 3.03 microM fibrinogen and 0.91 u/ml thrombin was determined for equilibrium systems. With added Ca2+, tau decreases, from tau 0 at 0 added Ca2+ (mean, 29.7 +/- 3 s), by approximately 3 s at 5 mM added Ca2+. Wi...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90305-4

    authors： Perizzolo KE,Sullivan S,Waugh DF

    更新日期：1985-03-01 00:00:00

  • Significance of H461 at subsite +1 in substrate binding and transglucosylation activity of amylomaltase from Corynebacterium glutamicum.

    abstract：:Amylomaltase (AM) catalyzes inter- and intra-molecular transglycosylation reactions of glucan to yield linear and cyclic oligosaccharide products. The functional roles of the conserved histidine at position 461 in the active site of AM from Corynebacterium glutamicum (CgAM) was investigated. H461 A/S/D/R/W were constr...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2018.06.002

    authors： Tumhom S,Krusong K,Kidokoro SI,Katoh E,Pongsawasdi P

    更新日期：2018-08-15 00:00:00

  • Kinetic mechanism of activation of muscle glycogen phosphorylase b by adenosine 5'-monophosphate.

    abstract：:The kinetic analysis of the enzymatic reaction catalyzed by glycogen phosphorylase b from the rabbit skeletal muscle has been carried out in the direction of glycogen synthesis under the conditions of the saturation of the enzyme by glycogen. The applicability of 12 different variants of the Monod-Wyman-Changeux model...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1274

    authors： Klinov SV,Kurganov BI

    更新日期：1994-07-01 00:00:00

  • The intermediate filament protein, synemin, is an AKAP in the heart.

    abstract：:Targeting of protein kinase A (PKA) by A-kinase anchoring proteins (AKAPs) contributes to high specificity of PKA signaling pathways. PKA phosphorylation of myofilament and cytoskeletal proteins may regulate myofibrillogenesis and myocyte remodeling during heart disease; however, known cardiac AKAPs do not localize to...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2006.06.010

    authors： Russell MA,Lund LM,Haber R,McKeegan K,Cianciola N,Bond M

    更新日期：2006-12-15 00:00:00

  • Effects of fumonisin B1 and (hydrolyzed) fumonisin backbone AP1 on membranes: a spin-label study.

    abstract：:Electron spin resonance (ESR) spectroscopy and spin label techniques have been used to study the effects of fumonisin B1 (FB1) and hydrolyzed fumonisin backbone (AP1) on the structural and dynamic properties of phosphatidylcholine membranes at the molecular level. Multilamellar liposomes consisting of dimyristoylphosp...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0477

    authors： Yin JJ,Smith MJ,Eppley RM,Troy AL,Page SW,Sphon JA

    更新日期：1996-11-01 00:00:00

  • Unified analysis of optical absorption spectra of carotenoids based on a stochastic model.

    abstract：:The chemical structures of the carotenoid molecules are very simple and one might think that their electronic features are easily predicted. However, there is still has so much unknown information excepting the correlation between the electronic energy state and the length of effective conjugation chain of carotenoids...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2018.04.021

    authors： Uragami C,Saito K,Yoshizawa M,Molnár P,Hashimoto H

    更新日期：2018-07-15 00:00:00

  • Comparative kinetic studies of the dealkylation reaction and steroid hydroxylations in various oxygen and carbon monoxide:oxygen atmospheres.

    abstract：:The time-course kinetics of the cytochrome P-450-catalyzed dealkylations of the exogenous compounds benzphetamine, ethylmorphine, codeine, and 7-ethoxycoumarin were compared to the hydroxylation of the endogenous compound testosterone. Using liver microsomes from phenobarbital-induced rats, the time course of the deme...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90249-7

    authors： Hamill SJ,Cooper DY,Schleyer H,Rosenthal O

    更新日期：1983-07-15 00:00:00

  • Properties of the lectin from the hog peanut (Amphicarpaea bracteata).

    abstract：:An N-acetyl-D-galactosamine-specific lectin has been isolated from the two seed forms of the hog peanut (Amphicarpaea bracteata) using an affinity support containing the synthetic type A blood group trisaccharide alpha-D-GalNAc-(1,3)-[alpha-L-Fuc-(1,2)]-beta-D-Gal (Synsorb A). The affinity-purified lectin appears to b...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90310-9

    authors： Maliarik MJ,Roberts DD,Goldstein IJ

    更新日期：1987-05-15 00:00:00

  • Production of monoclonal antibodies with specificity for different epitopes on the human epidermal growth factor molecule.

    abstract：:Four different monoclonal antibodies (MAbs) to human epidermal growth factor (hEGF) were obtained from mouse hybridoma cell lines. They were highly specific for hEGF and did not cross-react appreciably with mouse EGF. The immunoglobulin class of these MAbs was IgG1 or IgG2a. The apparent Kd values for hEGF of these MA...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90656-x

    authors： Yoshitake Y,Nishikawa K

    更新日期：1988-06-01 00:00:00

  • ΔNp63α/IRF6 interplay activates NOS2 transcription and induces autophagy upon tobacco exposure.

    abstract：:Tobacco-induced oxidative stress leads to chronic inflammation and is implicated in the development of many human epithelial cancers, including head and neck cancer. Cigarette smoke exposure was shown to induce the expression of the ΔNp63α and nitric oxide synthase (NOS)-2 in head and neck squamous cell carcinoma cell...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2010.11.020

    authors： Ratovitski EA

    更新日期：2011-02-15 00:00:00

  • Macrophage recognition of externalized phosphatidylserine and phagocytosis of apoptotic Jurkat cells--existence of a threshold.

    abstract：:Phosphatidylserine (PS) is predominantly confined to the inner leaflet of plasma membrane in cells, but it is externalized on the cell surface during apoptosis. This externalized PS is required for effective phagocytosis of apoptotic cells by macrophages. Because PS trans-bilayer asymmetry is not absolute in different...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/s0003-9861(03)00083-3

    authors： Borisenko GG,Matsura T,Liu SX,Tyurin VA,Jianfei J,Serinkan FB,Kagan VE

    更新日期：2003-05-01 00:00:00

  • Mechanism-based inactivation of human leukocyte elastase via an enzyme-induced sulfonamide fragmentation process.

    abstract：:We describe herein the design and in vitro biochemical evaluation of a novel class of mechanism-based inhibitors of human leukocyte elastase (HLE) that inactivate the enzyme via an unprecedented enzyme-induced sulfonamide fragmentation cascade. The inhibitors incorporate in their structure an appropriately functionali...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2004.05.018

    authors： Wei L,Lai Z,Gan X,Alliston KR,Zhong J,Epp JB,Tu J,Perera AB,Van Stipdonk M,Groutas WC

    更新日期：2004-09-01 00:00:00

  • Prostaglandin H synthase-1: evaluation of C-terminus function.

    abstract：:The first committed step in prostaglandin biosynthesis is catalyzed by prostaglandin H synthase, an enzyme localized to the endoplasmic reticulum (ER) membrane in a variety of cells. Several types of C-terminal region peptide sequence motifs have been found to lead to ER retention of other proteins. We have tested the...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1100

    authors： Ren Y,Loose-Mitchell DS,Kulmacz RJ

    更新日期：1995-02-01 00:00:00

  • Distribution and activity of alternatively spliced Alzheimer amyloid peptide precursor and scrapie PrP mRNAs on rat brain polysomes.

    abstract：:Mammalian brains contain low levels of the Alzheimer amyloid precursor variants (AAPPs) and the normal form of the scrapie agent protease-resistant protein (PrPc); however, their mRNAs are readily detectable. To understand these discrepancies we have investigated some aspects of the translational regulation of these m...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90161-b

    authors： Denman R,Potempska A,Wolfe G,Ramakrishna N,Miller DL

    更新日期：1991-07-01 00:00:00

  • Effect of nitrous oxide inactivation of vitamin B12 on the levels of folate coenzymes in rat bone marrow, kidney, brain, and liver.

    abstract：:The effects of nitrous oxide inactivation of the vitamin B12-dependent enzyme, methionine synthetase, on the distribution of folic acid derivatives in rat bone marrow cells, kidney, brain, and liver were determined. Methionine synthetase activity was decreased by about 90% in bone marrow cells, kidney, and brain and b...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(86)90114-1

    authors： Wilson SD,Horne DW

    更新日期：1986-01-01 00:00:00

  • Resonance Raman spectroscopic evidence for an anionic flavin semiquinone in bovine liver monoamine oxidase.

    abstract：:The flavoprotein monoamine oxidase B (MAO B) from bovine liver, as isolated, has an unusual additional absorption band at 412 nm, which is similar to the absorption of its anionic flavin semiquinone form, (Fl.-), and other typical (Fl.-) flavoproteins. Denaturation of the enzyme results in the elimination of this anom...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1993.1025

    authors： Yue KT,Bhattacharyya AK,Zhelyaskov VR,Edmondson DE

    更新日期：1993-01-01 00:00:00

  • Identification of the FTBL protein of Sumner and Dounce as a leucine aminopeptidase.

    abstract：:The crystalline beef liver protein of Sumner and Dounce (A. L. Dounce, P. Z. Allen, and G. A. Mourtzikos (1978) Arch. Biochem. Biophys. 188, 251-265) termed FTBL (football) protein because of the shape of its crystals, has been identified as a crystalline leucine aminopeptidase (LAP), on the basis of its high specific...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90537-6

    authors： Dounce AL,Allen PZ

    更新日期：1987-08-15 00:00:00