Electron microscopic localization of pyruvate carboxylase in rat liver and Saccharomyces cerevisiae by immunogold procedures.

Abstract:

:The intracellular location of pyruvate carboxylase (EC 6.4.1.1) in rat liver and Saccharomyces cerevisiae was investigated using the antibody-gold and protein A-gold techniques carried out as a postembedding immunoelectron microscopic procedure. The vast majority of gold particles (greater than 98%), indicative of the presence of antigenic sites of pyruvate carboxylase, were found in the mitochondria of rat liver. No other cellular compartment was labeled except the cytosol which did not account for more than 2% of the total labeling of a rat hepatocyte. Furthermore, 60% of labeled pyruvate carboxylase molecules within a mitochondrion were found adjacent to the matrix side of the inner mitochondrial membrane. In contrast, in S. cerevisiae, pyruvate carboxylase was found exclusively in the cytosol.

journal_name

Arch Biochem Biophys

authors

Rohde M,Lim F,Wallace JC

doi

10.1016/0003-9861(91)90608-l

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

197-201

issue

1

eissn

0003-9861

issn

1096-0384

pii

0003-9861(91)90608-L

journal_volume

290

pub_type

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