Electron microscopic localization of pyruvate carboxylase in rat liver and Saccharomyces cerevisiae by immunogold procedures.


:The intracellular location of pyruvate carboxylase (EC in rat liver and Saccharomyces cerevisiae was investigated using the antibody-gold and protein A-gold techniques carried out as a postembedding immunoelectron microscopic procedure. The vast majority of gold particles (greater than 98%), indicative of the presence of antigenic sites of pyruvate carboxylase, were found in the mitochondria of rat liver. No other cellular compartment was labeled except the cytosol which did not account for more than 2% of the total labeling of a rat hepatocyte. Furthermore, 60% of labeled pyruvate carboxylase molecules within a mitochondrion were found adjacent to the matrix side of the inner mitochondrial membrane. In contrast, in S. cerevisiae, pyruvate carboxylase was found exclusively in the cytosol.


Arch Biochem Biophys


Rohde M,Lim F,Wallace JC




Has Abstract


1991-10-01 00:00:00














  • Cardiotonic actions of quercetin and its metabolite tamarixetin through a digitalis-like enhancement of Ca2+ transients.

    abstract::The plant-derived flavonoid, quercetin (QCT), has many biological actions, including cardioprotective actions, resulting from its antioxidant and anti-inflammatory effects. In this study, effects of QCT and its metabolites on the contraction and Ca2+ transients (CaT) of mouse single cardiomyocytes were simultaneously ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hayamizu K,Morimoto S,Nonaka M,Hoka S,Sasaguri T

    更新日期:2018-01-01 00:00:00

  • Site-directed mutagenesis of the GDP binding domain of bacterial elongation factor Tu.

    abstract::The tertiary structure model of EF-Tu predicts that the amino acid sequence Val-Asp-His-Gly-Lys-Thr-Thr-Leu (residues 20-27) forms a pocket that binds the pyrophosphate group. To test this model we used site-directed mutagenesis to produce forms of EF-Tu altered in this region. The following mutations were constructed...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hwang YW,McCabe PG,Innis MA,Miller DL

    更新日期:1989-11-01 00:00:00

  • Inhibition of protein tyrosine phosphatases PTP1B and CD45 by sulfotyrosyl peptides.

    abstract::Sulfotyrosyl peptides corresponding to the known high-affinity substrate phosphotyrosyl peptide sequences in casein and the autophosphorylation sites of insulin receptor and EGF receptor were investigated as inhibitors of protein tyrosine phosphatases PTP1B and CD45. These peptides inhibit both PTP1B and CD45 in the m...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Desmarais S,Jia Z,Ramachandran C

    更新日期:1998-06-15 00:00:00

  • Natural agents: cellular and molecular mechanisms of photoprotection.

    abstract::The skin is the largest organ of the body that produces a flexible and self-repairing barrier and protects the body from most common potentially harmful physical, environmental, and biological insults. Solar ultraviolet (UV) radiation is one of the major environmental insults to the skin and causes multi-tiered cellul...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Afaq F

    更新日期:2011-04-15 00:00:00

  • New analogs of 2-methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 with conformationally restricted side chains: evaluation of biological activity and structural determination of VDR-bound conformations.

    abstract::We have successfully prepared E- and Z- isomers of 17-20 dehydro analogs of 2-methylene-19-nor-(20S)-1alpha,25-dihydroxyvitamin D3 (2MD). Both isomers bind to the recombinant rat vitamin D receptor (VDR) with high affinity. The Z-isomer (Vit-III 17-20Z) displays activity in vivo and in vitro that is similar to 2MD. Th...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Vanhooke JL,Tadi BP,Benning MM,Plum LA,DeLuca HF

    更新日期:2007-04-15 00:00:00

  • The hepatic Ah receptor for 2,3,7,8-tetrachlorodibenzo-p-dioxin: species differences in subunit dissociation.

    abstract::2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD, dioxin) produces many of its biological effects by binding to a soluble, intracellular protein (the Ah receptor (AhR]. The hepatic AhR, from a variety of species, is present in low salt cytosol as a form which sediments at 8-10 S. High salt (0.4 M KCL) dissociates the rat, gu...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Denison MS,Vella LM

    更新日期:1990-03-01 00:00:00

  • Nucleoside triphosphate synthesis catalysed by adenylate kinase is ADP dependent.

    abstract::Adenylate kinase (Adk) that catalyses the synthesis of ADP from ATP and AMP has also been shown to perform an ATP dependent phosphorylation of ribo- and deoxynucleoside diphosphates to their corresponding nucleoside triphosphate; ATP+(d)NDP<-->ADP+(d)NTP. This reaction, suggested to occur by the transfer of the gamma-...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Willemoës M,Kilstrup M

    更新日期:2005-12-15 00:00:00

  • The mouse plasma membrane Ca2+ pump isoform 1 promoter: cloning and characterization.

    abstract::The expression of plasma membrane Ca2+ pump (PMCA) is regulated by various hormones or agonists via multiple second messenger pathways. Two different 5' segments of the PMCA1 gene (isoform 1) were cloned from a mouse genomic library. While one segment contained the 3' end of intron 1 and exon 2, the other segment was ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Du Y,Carlock L,Kuo TH

    更新日期:1995-01-10 00:00:00

  • Molecular mechanism of the inhibition of cytochrome c aggregation by Phe-Gly.

    abstract::Experimental and computational studies suggest that few general principles govern protein/protein interactions and aggregation. The knowledge of these rules may be exploited to design peptides that are able to interfere with the self-assembly and aggregation of proteins. This work is aimed to verify the validity of th...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: La Rosa C,Milardi D,Amato E,Pappalardo M,Grasso D

    更新日期:2005-03-01 00:00:00

  • Stimulation of AMP-activated protein kinase (AMPK) is associated with enhancement of Glut1-mediated glucose transport.

    abstract::In cells expressing only the Glut1 isoform of glucose transporters, we have shown that glucose transport is markedly stimulated in response to hypoxia or inhibition of oxidative phosphorylation, conditions that would be expected to cause a stimulation of AMP-activated protein kinase (AMPK) activity. In the present stu...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Abbud W,Habinowski S,Zhang JZ,Kendrew J,Elkairi FS,Kemp BE,Witters LA,Ismail-Beigi F

    更新日期:2000-08-15 00:00:00

  • The bacterial hemoglobin from Vitreoscilla can support the aerobic growth of Escherichia coli lacking terminal oxidases.

    abstract::Two Escherichia coli mutants that lack both cytochrome o and d terminal oxidases are able to grow with glucose as the carbon source but not with the aerobic substrates succinate or lactate. One of these, GV101, is a deletion mutant of cytochrome o and a point mutation of cytochrome d. The other, GK100, is a total dele...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Dikshit RP,Dikshit KL,Liu YX,Webster DA

    更新日期:1992-03-01 00:00:00

  • Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.

    abstract::The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions. Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation. To understand ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Shahid S,Ahmad F,Hassan MI,Islam A

    更新日期:2015-10-15 00:00:00

  • Changes in phosphofructokinase isozymes during development of myoblasts to myotubes.

    abstract::The regulation of phosphofructokinase during development of C2C12 myoblasts to myotubes was investigated. Enzyme activity was markedly increased during myogenic development. The increase was observed when enzyme activity was measured under optimal conditions and was not due to changes in the allosteric kinetic propert...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wills KN,Mansour TE

    更新日期:1990-04-01 00:00:00

  • Insight into the apoptosis-inducing action of alpha-bisabolol towards malignant tumor cells: involvement of lipid rafts and Bid.

    abstract::In a precedent report we showed that alpha-bisabolol, a sesquiterpene present widely in the plant kingdom, exerts a rapid and efficient apoptosis-inducing action selectively towards human and murine malignant glioblastoma cell lines through mitochondrial damage. The present study extends these data demonstrating the a...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Darra E,Abdel-Azeim S,Manara A,Shoji K,Maréchal JD,Mariotto S,Cavalieri E,Perbellini L,Pizza C,Perahia D,Crimi M,Suzuki H

    更新日期:2008-08-15 00:00:00

  • Structural alterations of the inner mitochondrial membrane in ischemic liver cell injury.

    abstract::Mitoplasts were prepared from 3-h ischemic livers in an attempt to define the structural alterations in the inner membrane that may account for the functional deficiencies of ischemic mitochondria. Mitoplasts from both control and ischemic livers had similar specific activities of cytochrome oxidase and succinate-cyto...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Okayasu T,Curtis MT,Farber JL

    更新日期:1985-02-01 00:00:00

  • Crystal structure and tartrate inhibition of Legionella pneumophila histidine acid phosphatase.

    abstract::Histidine acid phosphatases (HAPs) utilize a nucleophilic histidine residue to catalyze the transfer of a phosphoryl group from phosphomonoesters to water. HAPs function as protein phosphatases and pain suppressors in mammals, are essential for Giardia lamblia excystation, and contribute to virulence of the category A...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Dhatwalia R,Singh H,Reilly TJ,Tanner JJ

    更新日期:2015-11-01 00:00:00

  • Regulation of neutral cholesteryl esterase in arterial smooth muscle cells: stimulation by agonists of adenylate cyclase and cyclic AMP-dependent protein kinase.

    abstract::Cultured arterial smooth muscle cells have been found to contain an activatable neutral cholesteryl esterase (EC This enzyme is similar to that previously described in adipose tissue, adrenal cortex, and aortic homogenates. Although both the lysosomal (acid) and cytoplasmic (neutral) cholesteryl esterases w...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hajjar DP

    更新日期:1986-05-15 00:00:00

  • Purification and characterization of the sesquiterpene cyclase aristolochene synthase from Penicillium roqueforti.

    abstract::The sesquiterpene cyclase, aristolochene synthase, has been purified from Penicillium roqueforti by gel filtration and anion-exchange chromatography. Isolation was facilitated by a change in the elution behavior of the enzyme during gel filtration at different steps in the purification. The purified enzyme had a speci...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hohn TM,Plattner RD

    更新日期:1989-07-01 00:00:00

  • Induction of the male-specific cytochrome P450 3A2 in female rats by phenytoin.

    abstract::We previously reported that administration of dexamethasone (DEX) and other selected pharmacological agents to rats resulted in a profound increase in hepatic cytochrome P450 3A1 in both sexes, but male constitutive P450 3A2 was modestly increased (4-fold) in adult males and not detected in either treated or untreated...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ghosal A,Sadrieh N,Reik L,Levin W,Thomas PE

    更新日期:1996-08-01 00:00:00

  • 60S ribosomal protein L35 regulates β-casein translational elongation and secretion in bovine mammary epithelial cells.

    abstract::60S ribosomal protein L35 (RPL35) is an important component of the 60S ribosomal subunit and has a role in protein translation and endoplasmic reticulum (ER) docking. However, few studies have investigated RPL35 in eukaryotes and much remains to be learned. Here, we analyzed the function of RPL35 in β-casein (CSN2) sy...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Jiang N,Hu L,Liu C,Gao X,Zheng S

    更新日期:2015-10-01 00:00:00

  • Antioxidant potentials of nanoceria synthesized by solution plasma process and its biocompatibility study.

    abstract::Nanoceria were synthesized by discharging plasma at 800 V with a frequency of 30 kHz for 0-25 min using a pulsed unipolar power supply into solutions containing 1 or 2 mM of Ce(NO3)2. UV-Vis spectroscopy showed a characteristic absorbance maxima at 304-320 nm for the nanoceria with increase in the intensity of the pea...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Davoodbasha M,Park BR,Rhee WJ,Lee SY,Kim JW

    更新日期:2018-05-01 00:00:00

  • Eicosapentaenoic acid-containing polar lipids from seaweed Susabinori (Pyropia yezoensis) alleviate hepatic steatosis in obese db/db mice.

    abstract::Nonalcoholic fatty liver disease (NAFLD) is emerging as the most common liver disease in industrialized countries. Because hepatic steatosis is an early pathogenesis of NAFLD, the discovery of food components that could ameliorate hepatic steatosis is of interest. Susabinori (Pyropia yezoensis) is recognized as one of...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Yanagita T,Tsuge K,Koga M,Inoue N,Nagao K

    更新日期:2020-09-30 00:00:00

  • Two phosphorylated forms of myosin in thrombin-stimulated platelets.

    abstract::Three forms of 20-kDa myosin light chain (MLC), unphosphorylated, monophosphorylated, and diphosphorylated MLC (designated 20K, 20K-P, and 20K-PP) were demonstrated in thrombin-stimulated human platelets by two different gel electrophoretic methods: in the presence of glycerol urea or in two dimensions (isoelectric an...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Naka M,Saitoh M,Hidaka H

    更新日期:1988-03-01 00:00:00

  • Kinetic and hysteretic behavior of ATP hydrolysis of the highly stable dimeric ATP synthase of Polytomella sp.

    abstract::The F1FO-ATP synthase of the colorless alga Polytomella sp. exhibits a robust peripheral arm constituted by nine atypical subunits only present in chlorophycean algae. The isolated dimeric enzyme exhibits a latent ATP hydrolytic activity which can be activated by some detergents. To date, the kinetic behavior of the a...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Villavicencio-Queijeiro A,Pardo JP,González-Halphen D

    更新日期:2015-06-01 00:00:00

  • Nitroxyl (NO-): a substrate for superoxide dismutase.

    abstract::The interactions of Cu, Zn superoxide dismutase (SOD) with nitroxyl (NO-) and nitric oxide (NO), both of which are thought to be biologically significant, have been studied but remain undefined. Having previously noted that NO- can reduce Cu (II), Zn SOD aerobically, we now report that it also can do so anaerobically ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liochev SI,Fridovich I

    更新日期:2002-06-15 00:00:00

  • Incorporation of serine into the phospholipids of phosphatidylethanolamine-depleted Tetrahymena.

    abstract::Phosphatidylserine formation and decarboxylation are decreased in Tetrahymena in which phosphatidylethanolamine has been replaced by its isosteric analog 3-aminopropylphosphonolipid (1,2-diacylglyceryl-3-O-(3-aminopropylphosphonate). The combined activity of the phosphatidylethanolamine: serine phosphatidyltransferase...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Smith JD

    更新日期:1984-05-01 00:00:00

  • Interleukin-2 is one of the targets of 1,25-dihydroxyvitamin D3 in the immune system.

    abstract::Interleukin (IL)-2 knockout (KO) mice, which spontaneously develop symptoms of inflammatory bowel disease similar to ulcerative colitis in humans, were made vitamin D deficient (D-) or vitamin D sufficient (D+) or were supplemented with 1,25-dihydroxyvitamin D(3) (1,25D3). 1,25-Dihydroxyvitamin D3 supplementation, but...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Bemiss CJ,Mahon BD,Henry A,Weaver V,Cantorna MT

    更新日期:2002-06-15 00:00:00

  • Effects of isoflurane on voltage-dependent calcium fluxes in rabbit T-tubule membranes: comparison with alcohols.

    abstract::The effects of racemic (+/-) and (+)- and (-)-stereoisomers of isoflurane on depolarization-induced (45)Ca(2+) fluxes mediated by voltage-dependent Ca(2+) channels were investigated in transverse tubule membrane vesicles from rabbit skeletal muscle. In the concentration range 0.5 to 2 mM, (+/-)-isoflurane inhibited (4...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Oz M,Tchugunova Y,Dinc M,Dunn SM

    更新日期:2002-02-15 00:00:00

  • Thermodynamics of the reactions of carbamoyl phosphate.

    abstract::Two measurements of equilibrium constants by Marshall and Cohen make it possible to calculate standard Gibbs energies of formation of the species of carbamate and carbamoyl phosphate. Carbamate formation from carbon dioxide and ammonia does not require an enzyme, and the equilibrium concentrations of carbamate in ammo...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Alberty RA

    更新日期:2006-07-01 00:00:00

  • Woodchuck VEGF (wVEGF) characteristics: Model for angiogenesis and human hepatocellular carcinoma directed therapies.

    abstract::Vascular endothelial growth factor (VEGF) stimulates angiogenesis. Human hepatocellular carcinoma (HCC) is a VEGF-driven tumor often associated with chronic hepatitis B or C virus infection. The woodchuck is a well-characterized model of hepatitis B virus related HCC and a valuable tool for translational studies of no...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Huang H,Salavaggione O,Rivera L,Mukherjee S,Brekken R,Tennant B,Iyer R,Adjei A

    更新日期:2019-01-01 00:00:00