Inhibitory effect of the polyanionic drug suramin on the in vitro HIV DNA integration reaction.

abstract：:Among the biologically and pharmacologically relevant nitrogen oxides, nitroxyl (HNO) remains one of the most poorly studied and least understood. Several previous reports indicate that thiols may be a primary target for the biological actions of HNO. However, the intimate details of the chemical interaction of HNO wi...

journal_title：Archives of biochemistry and biophysics

authors： Cook NM,Shinyashiki M,Jackson MI,Leal FA,Fukuto JM

更新日期：2003-02-01 00:00:00

abstract：:Cultured arterial smooth muscle cells have been found to contain an activatable neutral cholesteryl esterase (EC 3.1.1.13). This enzyme is similar to that previously described in adipose tissue, adrenal cortex, and aortic homogenates. Although both the lysosomal (acid) and cytoplasmic (neutral) cholesteryl esterases w...

journal_title：Archives of biochemistry and biophysics

authors： Hajjar DP

更新日期：1986-05-15 00:00:00

abstract：:The facultative intracellular bacterial pathogen Listeria monocytogenes invades and multiplies in many mammalian cell types. During the interaction with its host cells it strongly interferes with and modulates host cell functions. In the present review we summarize the current knowledge on the modulation of signal tra...

journal_title：Archives of biochemistry and biophysics

authors： Kuhn M,Pfeuffer T,Greiffenberg L,Goebel W

更新日期：1999-12-01 00:00:00

abstract：:Cytosolic proteins may play an important role in the intracellular transport of bile acids in enterocytes. The lithocholate binding properties of cytosolic protein from bovine small intestine were studied. Lithocholate binding was observed in the Y (45-50 kDa), Y' (30-35 kDa), and Z fractions (10-15 kDa) following gel...

journal_title：Archives of biochemistry and biophysics

authors： Takikawa H,Arai S,Yamanaka M

更新日期：1992-11-01 00:00:00

abstract：:Vascular dysfunction and injurious stimuli such as oxidative stress is closely related to the risk of cardiovascular diseases (CVD). Dietary polyphenols is reported to exert the beneficial effects on reducing the risk of CVD. Black soybean is rich in polyphenols, including isoflavones, anthocyanidins and flavan-3-ols,...

journal_title：Archives of biochemistry and biophysics

authors： Yamashita Y,Wang L,Nakamura A,Nanba F,Saito S,Toda T,Nakagawa J,Ashida H

更新日期：2020-07-30 00:00:00

abstract：:The relationships between conformational dynamics, stability and protein function are not obvious. Frataxin (FXN) is an essential protein that forms part of a supercomplex dedicated to the iron-sulfur (Fe-S) cluster assembly within the mitochondrial matrix. In humans, the loss of FXN expression or a decrease in its fu...

journal_title：Archives of biochemistry and biophysics

authors： Castro IH,Bringas M,Doni D,Noguera ME,Capece L,Aran M,Blaustein M,Costantini P,Santos J

更新日期：2020-09-30 00:00:00

abstract：:Chloroplast tRNA(Glu) is a bifunctional molecule involved in both the early steps of chlorophyll synthesis and chloroplast protein biosynthesis. Recently the enzymes involved in these processes have been characterized from the green alga Chlamydomonas reinhardtii. In order to investigate whether transcription of the g...

journal_title：Archives of biochemistry and biophysics

authors： Jahn D

更新日期：1992-11-01 00:00:00

abstract：:The purpose of this study was to investigate the effects of thyroid state on rates and sites of H(2)O(2) production in rat muscle mitochondria. With Complex I- and Complex II-linked substrates, hypothyroidism decreased and hyperthyroidism increased the rates of O(2) consumption during State 4 and State 3 respiration a...

journal_title：Archives of biochemistry and biophysics

authors： Venditti P,Puca A,Di Meo S

更新日期：2003-03-01 00:00:00

abstract：:The kinetics of bovine Cu,Zn superoxide dismutase were studied by pulse radiolysis. To ensure the absence of catalytically active free copper, commercially obtained holo-superoxide dismutase was demetallated, and the apo-superoxide dismutase concentrations were determined by isothermal titration calorimetry prior to r...

journal_title：Archives of biochemistry and biophysics

authors： Michel E,Nauser T,Sutter B,Bounds PL,Koppenol WH

更新日期：2005-07-15 00:00:00

abstract：:Plastidial acyl-acyl carrier protein:sn-glycerol-3-phosphate acyltransferase (GPAT; EC 2.3.1.15) catalyzes the acyl-acyl carrier protein-dependent sn-1 acylation of sn-glycerol 3-phosphate (G3P) to produce lysophosphatic acid. Functional recombinant Erysimum asperum GPAT (EaGPAT), devoid of transit peptide, was produc...

journal_title：Archives of biochemistry and biophysics

authors： Chen X,Miles R,Snyder C,Truksa M,Zhang J,Shah S,Weselake RJ

更新日期：2014-07-15 00:00:00

abstract：:Both CYP158A1 and CYP158A2 are able to catalyze an oxidative C-C coupling reaction producing biflaviolin or triflaviolin in Streptomyces coelicolor A3(2). The substrate-bound crystal structures of CYP158A2 and CYP158A1 reveal that the side chain of Ile87 in CYP158A2 points to the active site contacting the distal flav...

journal_title：Archives of biochemistry and biophysics

authors： Zhao B,Bellamine A,Lei L,Waterman MR

更新日期：2012-02-15 00:00:00

abstract：:Sodium pump was the first ion pump discovered. A member of the family of active transporters that catalyze adenosine 5'-triphosphate hydrolysis by forming a phosphorylated enzyme intermediate, sodium pump couples the energy released to unequal countertransport of sodium and potassium ions. The ion gradient generated b...

journal_title：Archives of biochemistry and biophysics

authors： Faller LD

更新日期：2008-08-01 00:00:00

abstract：:NADH:cytochrome b5 oxidoreductase catalyzes the transfer of reducing equivalents from the physiological electron donor, NADH, to two molecules of cytochrome b5. Utilizing a heterologous expression system for the soluble, catalytic domain of the rat microsomal enzyme, we have produced two mutants, corresponding to E255...

journal_title：Archives of biochemistry and biophysics

authors： Davis CA,Barber MJ

更新日期：2004-05-15 00:00:00

abstract：:Arachidonate 12-lipoxygenase (12-LO) from porcine leukocytes was expressed in insect cells using a baculovirus expression vector. The recombinant 12-LO was expressed as an N-terminal fusion protein with a 31-amino acid polypeptide carrying a six-histidine tag and an enterokinase cleavage site. Maximal intracellular en...

journal_title：Archives of biochemistry and biophysics

authors： Reddy RG,Yoshimoto T,Yamamoto S,Funk CD,Marnett LJ

更新日期：1994-07-01 00:00:00

abstract：:Reperfusion of ischemic myocardial tissue results in an increase in mitochondrial free radical production and declines in respiratory activity. The effects of ischemia and reperfusion on the activities of Krebs cycle enzymes, as well as enzymes involved in electron transport, were evaluated to provide insight into whe...

journal_title：Archives of biochemistry and biophysics

authors： Sadek HA,Humphries KM,Szweda PA,Szweda LI

更新日期：2002-10-15 00:00:00

abstract：:Previous studies showed that vegetative cells of Dictyostelium discoideum make a cysteine proteinase called proteinase-1, which contains multiple residues of GlcNAc-1-P linked directly to peptidyl serines. As a prelude to understanding the function of this novel carbohydrate modification, we purified and extensively c...

journal_title：Archives of biochemistry and biophysics

authors： Mehta DP,Etchison JR,Freeze HH

更新日期：1995-08-01 00:00:00

abstract：BACKGROUND:The main functions of the skin are to protect against environmental insults and prevent water loss, which are performed by the complex lipid- and protein matrix present in the outermost layers of the epithelium. The lipidome of these outer layers is mainly composed of ceramides, fatty acids, and cholesterol,...

journal_title：Archives of biochemistry and biophysics

authors： Muresan XM,Narzt MS,Woodby B,Ferrara F,Gruber F,Valacchi G

更新日期：2019-05-15 00:00:00

abstract：:In this work we demonstrate that aldose reductase (AR) interacts directly with tubulin and, was subjected to microtubule formation conditions, enzymatic AR activity increased more than sixfold. Since AR interacts mainly with tubulin that has 3-nitro-tyrosine in its carboxy-terminal, we evaluated whether tyrosine and o...

journal_title：Archives of biochemistry and biophysics

authors： Rivelli JF,Ochoa AL,Santander VS,Nigra A,Previtali G,Casale CH

更新日期：2018-09-15 00:00:00

abstract：:An inhibitor protein of the catalytic subunit of the cyclic 3',5'-AMP-dependent protein kinase from the nematode Ascaris suum was isolated and characterized. The molecular weight of the inhibitor was estimated as 28,000 by electrophoresis under denaturing conditions and as 30,000 by gel permeation chromatography on Su...

journal_title：Archives of biochemistry and biophysics

authors： Hoffmann R,Jung S,Hofer HW

更新日期：1992-09-01 00:00:00

abstract：:Properly regulated cyclin proteolysis is critical for normal cell cycle progression. A nine-amino acid peptide motif called the destruction box (D box) is present at the N terminus of the yeast mitotic cyclins. This short sequence is required for cyclin ubiquitination and subsequent proteolysis. The anaphase-promoting...

journal_title：Archives of biochemistry and biophysics

authors： Meyn MA 3rd,Melloy PG,Li J,Holloway SL

更新日期：2002-11-15 00:00:00

abstract：:The effects of testosterone propionate (TP) on the oxidative stress and mitochondrial function, as well as on mitochondria associated apoptotic signaling, were analyzed in the gastric mucosa in orchiectomized male rats. The present study showed that testosterone deficiency triggered apoptosis by damaging the mitochond...

journal_title：Archives of biochemistry and biophysics

authors： Kang J,Jia Z,Ping Y,Liu Z,Yan X,Xing G,Yan W

更新日期：2018-07-01 00:00:00

abstract：:Ferredoxin reductase (Fd-reductase) supplies reducing equivalents obtained from NADPH to mitochondrial cytochrome P450 enzymes via the small iron-sulfur protein ferredoxin. Two cDNAs (differing by the presence or absence of an 18-bp insert in the coding region) for the human Fd-reductase were subcloned into a newly co...

journal_title：Archives of biochemistry and biophysics

authors： Brandt ME,Vickery LE

更新日期：1992-05-01 00:00:00

abstract：:In this report we provide data, for the first time, demonstrating the conversion of the heme moiety of certain cytochrome P-450 and P-420 preparations, to biliverdin, catalyzed by heme oxygenase. We have used purified preparations of cytochromes P-450c, P-450b, P-450/P-420c, or P-450/P-420b as substrates in a heme oxy...

journal_title：Archives of biochemistry and biophysics

authors： Kutty RK,Daniel RF,Ryan DE,Levin W,Maines MD

更新日期：1988-02-01 00:00:00

abstract：:Venom from the common tiger snake, Notechis scutatus scutatus, contains several toxic acidic proteins which promote hypotension and hemorrhage in mice. One of these toxins, HTe, has a phospholipase A2 (PLA2) amino acid sequence. It contains 125 amino acids rather than the 119/120 found in other N. s. scutatus PLA2s, b...

journal_title：Archives of biochemistry and biophysics

authors： Francis B,Coffield JA,Simpson LL,Kaiser II

更新日期：1995-04-20 00:00:00

abstract：:P-815 mouse mastocytoma cells express the K isozyme of pyruvate kinase and the specific activity of this enzyme is increased in response to N6,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate, 8-bromoadenosine 3':5'-cyclic monophosphate, cholera toxin, and epinephrine, all of which also elevate the intracellular con...

journal_title：Archives of biochemistry and biophysics

authors： Ngo JL,Ibsen KH

更新日期：1989-03-01 00:00:00

abstract：:Activity of the rat liver microsomal vitamin K-dependent carboxylase has been studied at various concentrations of detergent. The activity which could be solubilized by 0.25% Triton X-100 was low but could be greatly increased if vitamin K-deficient rats were given vitamin K a few minutes before they were killed. At h...

journal_title：Archives of biochemistry and biophysics

authors： Shah DV,Swanson JC,Suttie JW

更新日期：1983-04-01 00:00:00

abstract：:The hepatic cytosolic hemoprotein tryptophan 2,3-dioxygenase (TDO) is the rate-limiting enzyme in tryptophan catabolism and thus plays a key role in regulating the physiological flux of tryptophan into relevant metabolic pathways. The TDO protein is induced by corticosteroids such as dexamethasone (DEX) and is stabili...

journal_title：Archives of biochemistry and biophysics

authors： Ren S,Correia MA

更新日期：2000-05-01 00:00:00

abstract：:Factors influencing pyrroline 5-carboxylate (P5C) synthesis from glutamate by a subcellular fraction enriched in mitochondria of rat small intestinal mucosa have been studied. P5C synthesis decreased rapidly if this subcellular fraction was preincubated at 20 degrees C in the absence of substrates; this effect suggest...

journal_title：Archives of biochemistry and biophysics

authors： Henslee JG,Wakabayashi Y,Small C,Jones ME

更新日期：1983-10-15 00:00:00

abstract：:The effects of fluorescein isothiocyanate II (FITC) on the actions of insulin in rat adipocytes were studied. When adipocytes were incubated with FITC at pH 7.4 (2 mM agent, 8 min), the cells were completely deprived of their specific insulin-binding activity and rendered unresponsive to the hormone. The effect of FIT...

journal_title：Archives of biochemistry and biophysics

authors： Goto Y,Sumida Y,Flanagan JE,Robinson FW,Simpson IA,Cushman SW,Kono T

更新日期：1992-03-01 00:00:00

abstract：:To assess the indirect effects of snake venom metalloproteinases (SVMP) on host tissue local necrosis, we investigated the effect of the SVMP jararhagin on the gene expression profiles of human fibroblasts in vitro and mouse tissue in vivo. Two functional classes of up-regulated proteins, cell death and inflammatory d...

journal_title：Archives of biochemistry and biophysics

authors： Gallagher P,Bao Y,Serrano SM,Laing GD,Theakston RD,Gutiérrez JM,Escalante T,Zigrino P,Moura-da-Silva AM,Nischt R,Mauch C,Moskaluk C,Fox JW

更新日期：2005-09-01 00:00:00