Nitroxyl-mediated disruption of thiol proteins: inhibition of the yeast transcription factor Ace1.

abstract：:Resonance Raman spectroscopy (RRS) is a non-invasive method that has been developed to assess carotenoid status in human tissues including human skin in vivo. Skin carotenoid status has been suggested as a promising biomarker for human studies. This manuscript describes research done relevant to the development of thi...

journal_title：Archives of biochemistry and biophysics

authors： Mayne ST,Cartmel B,Scarmo S,Jahns L,Ermakov IV,Gellermann W

更新日期：2013-11-15 00:00:00

abstract：:Glycine residues are recognized as important structural determinants in nucleotide-binding domains of many enzymes. The functional significance of seven glycine residues invariant in all 22 eNTPDase sequences was therefore examined. Glycine-to-alanine mutants of eNTPDase3 were analyzed for nucleotidase activities and ...

journal_title：Archives of biochemistry and biophysics

authors： Kirley TL,Yang F,Ivanenkov VV

更新日期：2001-11-01 00:00:00

abstract：:Antisera against peptides from the extreme N- and C-terminal regions of the VDR were evaluated. The N-terminal antiserum Ab192 functioned as a general-purpose antibody, being able to supershift the rhVDR in heterodimeric or homodimeric binding complexes in the EMSA, and detect both recombinant and native forms of the ...

journal_title：Archives of biochemistry and biophysics

authors： Koszewski NJ,Reinhardt TA,Langub MC,Malluche HH,Horst RL

更新日期：1998-01-15 00:00:00

abstract：:Quantitative estimation of cytosolic Ca2+- and phospholipid-dependent protein kinase (PKC) activity was performed by polyacrylamide gel electrophoresis under nondenaturating conditions (PAGE). With this method less than 50 micrograms of cytosol protein can be accurately quantitated for PKC activity. The amount of cyto...

journal_title：Archives of biochemistry and biophysics

authors： Fabbro D,Jungmann RA,Eppenberger U

更新日期：1985-05-15 00:00:00

abstract：:Phosphoenolpyruvate carboxylase from maize leaves was inactivated by pyridoxal 5'-phosphate in the dark and in the light. A two-step reversible mechanism is proposed for inactivation in the dark, which involves the formation of a noncovalent complex prior to a Schiff base with amino groups of the enzyme. Spectral anal...

journal_title：Archives of biochemistry and biophysics

authors： Podesta FE,Iglesias AA,Andreo CS

更新日期：1986-05-01 00:00:00

abstract：:Electron spin resonance (ESR) spectroscopy and spin label techniques have been used to study the effects of fumonisin B1 (FB1) and hydrolyzed fumonisin backbone (AP1) on the structural and dynamic properties of phosphatidylcholine membranes at the molecular level. Multilamellar liposomes consisting of dimyristoylphosp...

journal_title：Archives of biochemistry and biophysics

authors： Yin JJ,Smith MJ,Eppley RM,Troy AL,Page SW,Sphon JA

更新日期：1996-11-01 00:00:00

abstract：:Using the polymerase chain reaction (PCR) a 0.8-kb product was amplified from cDNA made from the midgut and Malpighian tubules of fifth instar larvae of Helicoverpa virescens. This 0.8-kb PCR product was then used to isolate a clone of the B subunit of the V-type ATPase from a cDNA library made from the same tissues. ...

journal_title：Archives of biochemistry and biophysics

authors： Gill SS,Ross LS

更新日期：1991-11-15 00:00:00

abstract：:Pyruvate carboxylase deficiency was previously reported to be the biochemical lesion in a yeast mutant, designated pyc, which cannot utilize ethanol, acetate, pyruvate, aspartate, or oxaloacetate as the sole carbon source [C. Wills and T. Melham (1985) Arch. Biochem. Biophys. 236, 782-791; C. Wills et al. (1986) Arch....

journal_title：Archives of biochemistry and biophysics

authors： Lim F,Rohde M,Morris CP,Wallace JC

更新日期：1987-10-01 00:00:00

abstract：:An acid phosphatase from Brassica nigra (black mustard) leaf petiole cell-suspension cultures has been purified 1633-fold to a final specific activity of 1225 (mumols orthophosphate produced/min)/mg protein and near homogeneity. The native protein was a glycosylated monomer having a molecular mass of 60 kDa and a pI o...

journal_title：Archives of biochemistry and biophysics

authors： Duff SM,Lefebvre DD,Plaxton WC

更新日期：1991-04-01 00:00:00

abstract：:The site of superoxide production in spinach thylakoids was found to be the aprotic interior of the thylakoid membranes near the P700 chlorophyll a protein at the reaction center of photosystem I complexes. This conclusion was drawn from the following findings. (i) Cytochrome c reduction by illuminated thylakoids, whi...

journal_title：Archives of biochemistry and biophysics

authors： Takahashi M,Asada K

更新日期：1988-12-01 00:00:00

abstract：:The photooxidizing xanthene dye rose bengal is shown to induce rapid Ca2+ release from skeletal muscle sarcoplasmic reticulum (SR) vesicles. In the presence of light, nanomolar concentrations of rose bengal increase the Ca2+ permeability of the SR and stimulate the production of singlet oxygen (1O2). In the absence of...

journal_title：Archives of biochemistry and biophysics

authors： Stuart J,Pessah IN,Favero TG,Abramson JJ

更新日期：1992-02-01 00:00:00

abstract：:The Ku autoantigen is a heterodimeric protein of 70- and 83-kDa subunits, endowed with duplex DNA end-binding capacity and DNA helicase activity (Human DNA Helicase II, HDH II). HDH II/Ku is well established as the DNA binding component, the regulatory subunit as well as a substrate for the DNA-dependent protein kinas...

journal_title：Archives of biochemistry and biophysics

authors： Ochem AE,Rechreche H,Skopac D,Falaschi A

更新日期：2008-02-01 00:00:00

abstract：:Using ammonium sulfate precipitation, gel filtration, and affinity chromatography, inosine monophosphate (IMP) oxidoreductase (EC 1.2.1.14) was isolated from the soluble proteins of the plant cell fraction of nitrogen-fixing nodules of cowpea (Vigna unguiculata L. Walp). The enzyme, purified more than 140-fold with a ...

journal_title：Archives of biochemistry and biophysics

authors： Atkins CA,Shelp BJ,Storer PJ

更新日期：1985-02-01 00:00:00

abstract：:Conidiospores of wild type and two mutant strains of Neurospora crassa were grown on [3-13C]serine, [2-13C]glycine, or [13C]formate. Acid extracts of the mycelia were analyzed by 13C NMR for incorporation of the 13C label into choline, serine, and adenine. The goal was to elucidate the function of cytosolic serine hyd...

journal_title：Archives of biochemistry and biophysics

authors： Jeong SS,Schirch V

更新日期：1996-11-15 00:00:00

abstract：:Expression of several forms of cytochrome P450 2B and of P450 4B1 in rabbit kidney was investigated by cloning from cDNA libraries constructed with renal mRNA from animals treated with phenobarbital. Isolation and sequencing of several cDNAs demonstrated that: (i) cytochrome P450 2B-B0 can be found in rabbit kidney al...

journal_title：Archives of biochemistry and biophysics

authors： Ryan R,Grimm SW,Kedzie KM,Halpert JR,Philpot RM

更新日期：1993-08-01 00:00:00

abstract：:It has previously been found that there is a synergistic effect of free purine bases and low concentrations of dialyzed platelet extract on net synthesis of DNA in serum-starved fibroblast-like mouse L-cells. Experiments with a mutant line of L-cells that was deficient in hypoxanthine phosphoribosyl transferase (EC 2....

journal_title：Archives of biochemistry and biophysics

authors： Flodgaard H,Klenow H

更新日期：1985-06-01 00:00:00

abstract：:Piratoxins (PrTX) I and III are phospholipases A2 (PLA2s) or PLA2 homologue myotoxins isolated from Bothrops pirajai snake venom, which also induce myonecrosis, bactericidal activity against Escherichia coli, disruption of artificial membranes, and edema. PrTX-III is a catalytically active hemolytic and anticoagulant ...

journal_title：Archives of biochemistry and biophysics

authors： Soares AM,Andrião-Escarso SH,Bortoleto RK,Rodrigues-Simioni L,Arni RK,Ward RJ,Gutiérrez JM,Giglio JR

更新日期：2001-03-15 00:00:00

abstract：:Type III galactosemia is an inherited disease caused by mutations which affect the activity of UDP-galactose 4'-epimerase (GALE). We evaluated the impact of four disease-associated variants (p.N34S, p.G90E, p.V94M and p.K161N) on the conformational stability and dynamics of GALE. Thermal denaturation studies showed th...

journal_title：Archives of biochemistry and biophysics

authors： Pey AL,Padín-Gonzalez E,Mesa-Torres N,Timson DJ

更新日期：2014-11-15 00:00:00

abstract：:The allylamine class of antifungal compounds are specific inhibitors of squalene epoxidase (SE). However, depending on their chemical structure, allylamine derivatives can be highly selective for either fungal or mammalian SEs. All allylamines tested previously, irrespective of their selectivity, inhibit fungal SEs in...

journal_title：Archives of biochemistry and biophysics

authors： Favre B,Ryder NS

更新日期：1997-04-15 00:00:00

abstract：:Existing literature describing the stoichiometry and stability of complexes between A23187 and divalent cations in solution has been extended to include additional transition series cations, the heavy-metal cations Cd2+ and Pb2+, plus seven lanthanide series trivalent cations. Stability constants of 1:1 complexes betw...

journal_title：Archives of biochemistry and biophysics

authors： Chapman CJ,Puri AK,Taylor RW,Pfeiffer DR

更新日期：1990-08-15 00:00:00

abstract：:The ability for various ligands to modulate the binding of fructose 1,6-bisphosphate (Fru-1,6-P2) with purified rat liver pyruvate kinase was examined. Binding of Fru-1,6-P2 with pyruvate kinase exhibits positive cooperativity, with maximum binding of 4 mol Fru-1,6-P2 per enzyme tetramer. The Hill coefficient (nH), an...

journal_title：Archives of biochemistry and biophysics

authors： Blair JB,Walker RG

更新日期：1984-07-01 00:00:00

abstract：:The total lipid composition of highly purified plasma membranes from light-grown barley (Hordeum vulgare) leaves was investigated. The plasma membranes were separated from intracellular membranes by subfractionation of the microsomal fraction using aqueous polymer two-phase partitioning. A novel finding was that gluco...

journal_title：Archives of biochemistry and biophysics

authors： Rochester CP,Kjellbom P,Andersson B,Larsson C

更新日期：1987-06-01 00:00:00

abstract：:(6R)-5,6,7,8-Tetrahydro-L-biopterin (R-THBP) is a cofactor not only for aromatic amino acid hydroxylases in mammalian tissues but also for nitric oxide synthase (NOS) induced by endotoxins or cytokines in some kinds of cells. Recently it has been reported that nitric oxide (NO) has biological activity in endothelium a...

journal_title：Archives of biochemistry and biophysics

authors： Nunokawa Y,Ishihara T,Kanai T,Noguchi T

更新日期：1992-11-01 00:00:00

abstract：:Mitoplasts were prepared from 3-h ischemic livers in an attempt to define the structural alterations in the inner membrane that may account for the functional deficiencies of ischemic mitochondria. Mitoplasts from both control and ischemic livers had similar specific activities of cytochrome oxidase and succinate-cyto...

journal_title：Archives of biochemistry and biophysics

authors： Okayasu T,Curtis MT,Farber JL

更新日期：1985-02-01 00:00:00

abstract：:Cold plasma is partly ionized non-thermal plasma generated at atmospheric pressure. It has been recognized as an alternative approach in medicine for sterilization of wounds, promotion of wound healing, topical treatment of skin diseases with microbial involvement and treatment of cancer. Cold plasma used in wound the...

journal_title：Archives of biochemistry and biophysics

authors： Mohd Nasir N,Lee BK,Yap SS,Thong KL,Yap SL

更新日期：2016-09-01 00:00:00

abstract：:A class II Zn(2+)-dependent fructose-1,6-bisphosphate (FBP)- aldolase was purified from an overproducer strain of Escherichia coli and characterized by standard biochemical techniques and 13C NMR spectroscopy. The principal finding of these studies was identification, by 13C NMR spectroscopy, of an enzyme-bound reacti...

journal_title：Archives of biochemistry and biophysics

authors： Szwergold BS,Ugurbil K,Brown TR

更新日期：1995-02-20 00:00:00

abstract：:The thermodynamics of the drug-inhibitors acyclovir, ganciclovir, and 9-benzylguanine binding to human purine nucleoside phosphorylase (hsPNP) were determined from isothermal titration calorimetry as a function of the substrate phosphate ion (Pi) concentration from 0 to 0.125 M and temperature from 15 degrees C to 35 ...

journal_title：Archives of biochemistry and biophysics

authors： Todorova NA,Schwarz FP

更新日期：2008-12-15 00:00:00

abstract：:The topographical localization of the N-terminus of cytochrome b in the inner mitochondrial membrane was determined by mild proteolysis of the yeast mitochondrial cytochrome bc1 complex and identification of the proteolytic fragments derived from subunits of the complex with an established orientation in the inner mem...

journal_title：Archives of biochemistry and biophysics

authors： Beattie DS,Jenkins HC,Howton MM

更新日期：1994-07-01 00:00:00

abstract：:Cytochrome P450 reductase was purified to homogeneity from cell suspension cultures of the opium poppy Papaver somniferum, the enzyme was characterized (K(m) cytochrome c, 8.3 microM; K(m) NADPH, 4.2 microM; pH optimum, 8.0; M(r), 80 kDa), and the amino acid sequence of internal peptides was determined. Partial cDNA c...

journal_title：Archives of biochemistry and biophysics

authors： Rosco A,Pauli HH,Priesner W,Kutchan TM

更新日期：1997-12-15 00:00:00

abstract：:To evaluate the conformation of reduced HEL, the monoclonal antibodies HyC1 and HyC2, which recognize different conformational epitopes on native hen egg lysozyme (HEL), were used, and the kinetics of their interactions with native HEL, S-1,2-dicarboxyethylated HEL (DCE-HEL), and carboxymethylated Cys6 and Cys127 HEL ...

journal_title：Archives of biochemistry and biophysics

authors： Oda M,Kitai A,Murakami A,Nishimura M,Ohkuri T,Abe Y,Ueda T,Nakamura H,Azuma T

更新日期：2010-02-15 00:00:00