The interaction of Q analogs, particularly hydroxydecyl benzoquinone (idebenone), with the respiratory complexes of heart mitochondria.

abstract：:The so-called 90,000-Da heat shock protein (hsp90) from chicken liver has been purified and physically characterized in the presence of high levels of the serine phosphatase inhibitor fluoride. The protein is an elongated dimer with a molecular weight of 160,000 and a frictional ratio of 1.6. On two-dimensional electr...

journal_title：Archives of biochemistry and biophysics

authors： Iannotti AM,Rabideau DA,Dougherty JJ

更新日期：1988-07-01 00:00:00

abstract：:Uncoupling protein 2 (UCP2) was reported to be involved in insulin-glucose homeostasis, based on well established event that inhibition of UCP2 stimulates insulin secretion in pancreatic beta-cells. However, the role of UCP2 on insulin-stimulated glucose uptake in adipose tissue, which is an indispensable process in i...

journal_title：Archives of biochemistry and biophysics

authors： Zhou H,Zhao J,Zhang X

更新日期：2009-06-01 00:00:00

abstract：:Glycine residues are recognized as important structural determinants in nucleotide-binding domains of many enzymes. The functional significance of seven glycine residues invariant in all 22 eNTPDase sequences was therefore examined. Glycine-to-alanine mutants of eNTPDase3 were analyzed for nucleotidase activities and ...

journal_title：Archives of biochemistry and biophysics

authors： Kirley TL,Yang F,Ivanenkov VV

更新日期：2001-11-01 00:00:00

abstract：:Solubilized NADPH-cytochrome c (P450) reductase was purified to homogeneity from an extract of spearmint (Mentha spicata) glandular trichomes by dye-ligand interaction chromatography on Matrex-Gel Red A and affinity chromatography on 2', 5'-adenosine diphosphate agarose. SDS-PAGE of the purified enzyme preparation rev...

journal_title：Archives of biochemistry and biophysics

authors： Ponnamperuma K,Croteau R

更新日期：1996-05-01 00:00:00

abstract：:Because angiogenesis underlies the pathogenesis of numerous conditions (cancer, psoriasis, macular degeneration), there is a pressing need for continued investigations into angiogenic signaling and potential drug targets. Antiangiogenic agents can be classified as either direct or indirect. Direct antiangiogenics act ...

journal_title：Archives of biochemistry and biophysics

authors： Berrios RL,Arbiser JL

更新日期：2011-04-15 00:00:00

abstract：:A cyanide-insensitive superoxide dismutase was purified to electrophoretic homogeneity from leaves of the eucaryote, Ginkgo biloba L. A molecular mass of 47,000 was determined for the enzyme, which consisted of two subunits of equal size. The enzyme preparation contained two isoenzymes with isoelectric points of 5.25 ...

journal_title：Archives of biochemistry and biophysics

authors： Duke MV,Salin ML

更新日期：1985-11-15 00:00:00

abstract：:Horseradish peroxidase (EC 1.11.1.7) is shown to catalyze the aerobic oxidation of lysozyme, bovine serum albumin, and protamine adducts with glycolaldehyde at physiological pH. This reaction is accompanied by light emission, which is attributed to the generation of triplet species. The intensity of chemiluminescence ...

journal_title：Archives of biochemistry and biophysics

authors： de Medeiros MH,Bechara EJ

更新日期：1986-07-01 00:00:00

abstract：:NADP-malate dehydrogenase activity, the ratio of NADPH to NADP, and thioredoxin redox state in Zea mays chloroplasts were determined after various treatments. Following transfer from dark to light, NADP-malate dehydrogenase was activated more than 20-fold within 10 min while the proportion of pyridine nucleotide as NA...

journal_title：Archives of biochemistry and biophysics

authors： Rebeille F,Hatch MD

更新日期：1986-08-15 00:00:00

abstract：:Normal thymocytes and thymoma cells have been exposed to the toxic action of tert-butyl hydroperoxide (t-BOOH) to study the mechanisms of oxidative lethal cell injury and the sensitivity of normal and tumor cells to an oxidative stress. From our data, we can suggest that: (a) In thymoma cells two mechanisms can operat...

journal_title：Archives of biochemistry and biophysics

authors： Bartoli GM,Piccioni E,Agostara G,Calviello G,Palozza P

更新日期：1994-07-01 00:00:00

abstract：:Candida wickerhamii NRRL Y-2563 produced a cell-bound beta-glucosidase when grown in complex media containing 50 g of cellobiose per liter. The majority of the enzyme was located on the cell surface and was released into the supernatant upon treatment of intact cells with Zymolyase 60,000. Only about 10% of the total ...

journal_title：Archives of biochemistry and biophysics

authors： Freer SN

更新日期：1985-12-01 00:00:00

abstract：:The mitochondrial apoptosis pathway has been neatly ordered. Mitochondrial apoptosis is governed by Bcl-2 family proteins, and their respective contributions determine the release of cytochrome c. It is clear that, among the Bcl-2 family, BH3-only proteins are the triggers: activation of BH3-only proteins by apoptotic...

journal_title：Archives of biochemistry and biophysics

authors： Häcker G,Weber A

更新日期：2007-06-15 00:00:00

abstract：:Spontaneously beating isolated rat atria were subjected to 1 h of anoxia at 37 degrees C in various cardioplegic solutions. Contraction continued for different times upon initiation of anoxia, depending on the nature of the cardioplegic solution. Two hundred micromolar P1,P5-di(adenosine-5')pentaphosphate (Ap5A) stopp...

journal_title：Archives of biochemistry and biophysics

authors： Savabi F,Bessman SP

更新日期：1986-07-01 00:00:00

abstract：:Asthma is characterized by a predominant T(H)2 type immune response to airborne allergens. Controlling T(H)2 cell function has been proposed as therapy for this disease. We show here that ligands for the nuclear receptor peroxisome proliferator activated receptor (PPAR)gamma significantly reduced the immunological sym...

journal_title：Archives of biochemistry and biophysics

authors： Mueller C,Weaver V,Vanden Heuvel JP,August A,Cantorna MT

更新日期：2003-10-15 00:00:00

abstract：:Several 13C isotope effects of relevance to reactions involving carbamate and carbamoyl phosphate have been determined. The fractionation factor of carbamate relative to aqueous CO2 is 1.011; the equilibrium isotope effect on the reaction catalyzed by carbamate kinase is 0.9983. From these data we can calculate that t...

journal_title：Archives of biochemistry and biophysics

authors： Tipton PA,Cleland WW

更新日期：1988-01-01 00:00:00

abstract：:Accumulating evidence suggests that oxidative stress resulting in reactive oxygen species generation plays a pivotal role in neurodegenerative diseases, supporting the realization of the use of radical scavengers, metal chelator agents, such as the natural polyphenols for therapy. In this study, we have focused on spe...

journal_title：Archives of biochemistry and biophysics

authors： Weinreb O,Amit T,Youdim MB

更新日期：2008-08-15 00:00:00

abstract：:Interactions between loops 2, 2' and 4, known as the loop bundle, stabilize the active site of caspase-3. Loop 4 (L4) is of particular interest due to its location between the active site and the dimer interface. We have disrupted a salt bridge between K242 and E246 at the base of L4 to determine its role in overall c...

journal_title：Archives of biochemistry and biophysics

authors： Walters J,Swartz P,Mattos C,Clark AC

更新日期：2011-04-01 00:00:00

abstract：:Alkaline phosphatase (AP) was purified to over 90% homogeneity from rat osteosarcoma by acetone precipitation followed by chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxyapatite. The purified enzyme had a specific activity of 759 units/mg protein at its optimal pH (10.5), and a Km of 0.8 mM for p-nitroph...

journal_title：Archives of biochemistry and biophysics

authors： Nair BC,Majeska RJ,Rodan GA

更新日期：1987-04-01 00:00:00

abstract：:The structure of rabbit muscle pyruvate kinase crystallized as a complex with Mg2+, K+, and L-phospholactate (L-P-lactate) has been solved and refined to 2.7 A resolution. The crystals, grown from solutions of polyethylene glycol 8000 at pH 7.5, belong to the space group P2(1) and have unit cell parameters a = 144.4 A...

journal_title：Archives of biochemistry and biophysics

authors： Larsen TM,Benning MM,Wesenberg GE,Rayment I,Reed GH

更新日期：1997-09-15 00:00:00

abstract：:Studies have demonstrated that accumulation of mitochondrial tocopheroxyl radical, the primary oxidation product of alpha-tocopherol, accompanies rapid consumption of tocopherol. Enzyme-linked electron flow lowers both the steady-state concentration of the radical and the consumption of tocopherol. Reduction of tocoph...

journal_title：Archives of biochemistry and biophysics

authors： Maguire JJ,Kagan V,Ackrell BA,Serbinova E,Packer L

更新日期：1992-01-01 00:00:00

abstract：:Studies in eucaryotic cells (mainly animals and yeast) indicate that at least two DNA polymerases are involved in DNA replication at the level of the replication fork: DNA polymerase alpha, which is associated with DNA primase, is involved in the replication of the lagging strand; DNA polymerase delta, associated with...

journal_title：Archives of biochemistry and biophysics

authors： Richard MC,Litvak S,Castroviejo M

更新日期：1991-05-15 00:00:00

abstract：:Recombinant human lymphotoxin (rhLT) produced by CHO cells transfected with human LT genomic DNA was purified to homogeneity, but approximately 5% of the molecules were devoid of the last two amino terminal residues. A peptide N-glycosylated at Asn62 (Tr-45) and one partially O-glycosylated at Thr7 (Tr-14) on cleavage...

journal_title：Archives of biochemistry and biophysics

authors： Fukushima K,Watanabe H,Takeo K,Nomura M,Asahi T,Yamashita K

更新日期：1993-07-01 00:00:00

abstract：:A cDNA from Arabidopsis thaliana resembling S-formylglutathione hydrolase (SFGH), an enzyme with putative roles in formaldehyde detoxification in animals and microorganisms, has been cloned and expressed in Escherichia coli. The purified recombinant Arabidopsis enzyme (AtSFGH) was a dimer composed of 31-kDa subunits. ...

journal_title：Archives of biochemistry and biophysics

authors： Kordic S,Cummins I,Edwards R

更新日期：2002-03-15 00:00:00

abstract：:The effect of a protein matrix on the processing of glycoprotein glycans by Golgi enzymes from plant seedlings has been determined with an artificial glycoprotein system, comparing the processing rates of glycan-(biotinyl)Asn (or glycan-(biotinamidohexanoyl)Asn) substrates either free or bound to avidin. An analysis o...

journal_title：Archives of biochemistry and biophysics

authors： Lu Y,Wold F

更新日期：1991-04-01 00:00:00

abstract：:It has been proposed that negatively charged amino acids on the surface of reductase and positively charged amino acids on the surface of P450 mediate the binding of both proteins through electrostatic interactions. In this study, we used a site-directed mutagenesis approach to determine a role for two lysine residues...

journal_title：Archives of biochemistry and biophysics

authors： Cvrk T,Strobel HW

更新日期：2001-01-15 00:00:00

abstract：:Rabbit antibodies have been prepared against ERp61, ERp59, and ERp49, three protein components of rough endoplasmic reticulum (RER) purified from mineral oil-induced plasmacytoma 315 (MOPC-315) tissue. Analysis of subcellular fractions of MOPC-315 tissue by an immunoprecipitation procedure demonstrated that all three ...

journal_title：Archives of biochemistry and biophysics

authors： Lewis MJ,Mazzarella RA,Green M

更新日期：1986-03-01 00:00:00

abstract：:Troponin T (TnT) binds to tropomyosin (Tm) to anchor the troponin complex in the thin filament, and it thus serves as a vital link in the Ca(2+) regulation of striated muscle contraction. Pioneer work three decades ago determined that the T1 and T2 chymotryptic fragments of TnT each contains a Tm-binding site. A more ...

journal_title：Archives of biochemistry and biophysics

authors： Jin JP,Chong SM

更新日期：2010-08-15 00:00:00

abstract：:Replication of the human immunodeficiency virus type 1 (HIV-1) is inhibited by interferons (IFNs), in part through activity of the IFN-inducible protein kinase PKR. To escape this antiviral effect, HIV-1 has developed strategies for blocking PKR function. We have previously shown that the HIV-1 Tat protein can associa...

journal_title：Archives of biochemistry and biophysics

authors： Cai R,Carpick B,Chun RF,Jeang KT,Williams BR

更新日期：2000-01-15 00:00:00

abstract：:Site-directed mutagenesis was used to probe the role of glycine residue 336 in the regulatory properties of Escherichia coli ADP-glucose pyrophosphorylase. This residue was previously found to be changed from glycine to aspartate in the gene of an Escherichia coli mutant strain. The mutant enzyme had altered kinetic p...

journal_title：Archives of biochemistry and biophysics

authors： Meyer CR,Bork JA,Nadler S,Yirsa J,Preiss J

更新日期：1998-05-01 00:00:00

abstract：:Extracellular ATP is known to increase the membrane permeability of a variety of cells. Addition of ATP to human leukemic lymphocytes loaded with the Ca2+ indicator, fura-2, induced a rise in cytosolic Ca2+ concentration which was attenuated or absent in NaCl media compared with KCl, choline Cl, or NMG Cl media. In co...

journal_title：Archives of biochemistry and biophysics

authors： Wiley JS,Chen R,Wiley MJ,Jamieson GP

更新日期：1992-02-01 00:00:00

abstract：:cDNA for mouse cytochrome c oxidase subunit IV (COXIV) was isolated by screening mouse liver and kidney cDNA libraries with a bovine COXIV cDNA probe. The 679-nucleotide nearly full length cDNA codes for a 22-amino acid presequence and a 147-amino acid mature protein which show 77 to 95% positional identity with the p...

journal_title：Archives of biochemistry and biophysics

authors： Carter RS,Avadhani NG

更新日期：1991-07-01 00:00:00