Localization of the two tropomyosin-binding sites of troponin T.

abstract：:The relationship between erythroid delta-aminolevulinate (ALA) synthase and hepatic ALA synthase in rat was analyzed immunochemically, using antibodies directed against rat liver ALA synthase and against chicken liver ALA synthase. Rat erythroid ALA synthase showed no cross-reactivity with anti-liver ALA synthase anti...

journal_title：Archives of biochemistry and biophysics

authors： Yamamoto M,Fujita H,Watanabe N,Hayashi N,Kikuchi G

更新日期：1986-02-15 00:00:00

abstract：:An inorganic pyrophosphatase (PPase) was purified from thylakoid membrane of spinach leaves to electrophoretic purity by methods including detergent solubilization, ammonium sulfate fractionation, and successive chromatographic techniques. Current protocol yielded about 10% recovery of total activity with a 30-fold pu...

journal_title：Archives of biochemistry and biophysics

authors： Jiang SS,Fan LL,Yang SJ,Kuo SY,Pan RL

更新日期：1997-10-01 00:00:00

abstract：:Versiconal cyclase catalyzes the dehydration of versiconal to versicolorin B or versicolorin C [versicolorin B(C)]. The enzyme was purified from mycelia of Aspergillus parasiticus by DEAE-cellulose, hydroxylapatite, and Mono Q column chromatography. The protein contains two identical subunits of molecular weight 72,00...

journal_title：Archives of biochemistry and biophysics

authors： Lin BK,Anderson JA

更新日期：1992-02-14 00:00:00

abstract：:Normal thymocytes and thymoma cells have been exposed to the toxic action of tert-butyl hydroperoxide (t-BOOH) to study the mechanisms of oxidative lethal cell injury and the sensitivity of normal and tumor cells to an oxidative stress. From our data, we can suggest that: (a) In thymoma cells two mechanisms can operat...

journal_title：Archives of biochemistry and biophysics

authors： Bartoli GM,Piccioni E,Agostara G,Calviello G,Palozza P

更新日期：1994-07-01 00:00:00

abstract：:Leukotoxin (ltx) and isoleukotoxin (iltx) methyl esters, are metabolites of methyl linoleic acid, an essential fatty acid. They have been associated with acute respiratory distress syndrome. The observed toxicity of ltx and iltx is, in fact, due to the metabolism of the epoxides to their corresponding diols by soluble...

journal_title：Archives of biochemistry and biophysics

authors： Greene JF,Williamson KC,Newman JW,Morisseau C,Hammock BD

更新日期：2000-04-15 00:00:00

abstract：:Monoclonal antibodies have been successfully isolated which are isozyme-specific for cytochrome P450p (3A1) or P4501 (3A2), two members of the steroid-inducible cytochrome P450 subfamily exhibiting 89% amino acid sequence homology, and these antibodies show less than 5% cross-reaction with 11 other cytochromes P450 (P...

journal_title：Archives of biochemistry and biophysics

authors： Cooper KO,Reik LM,Jayyosi Z,Bandiera S,Kelley M,Ryan DE,Daniel R,McCluskey SA,Levin W,Thomas PE

更新日期：1993-03-01 00:00:00

abstract：:Previous genetic studies have indicated that the type L alpha-glucan phosphorylase (Pho1) has an essential role during the initiation process of starch biosynthesis during rice seed development. To gain insight into its role in starch metabolism, we characterized the enzymatic properties of the Pho1 recombinant form. ...

journal_title：Archives of biochemistry and biophysics

authors： Hwang SK,Nishi A,Satoh H,Okita TW

更新日期：2010-03-01 00:00:00

abstract：:P-815 mouse mastocytoma cells express the K isozyme of pyruvate kinase and the specific activity of this enzyme is increased in response to N6,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate, 8-bromoadenosine 3':5'-cyclic monophosphate, cholera toxin, and epinephrine, all of which also elevate the intracellular con...

journal_title：Archives of biochemistry and biophysics

authors： Ngo JL,Ibsen KH

更新日期：1989-03-01 00:00:00

abstract：:Human pregnancy zone protein (PZP) is a major pregnancy-associated plasma protein strongly related to alpha2-macroglobulin (alpha2-M). Both alpha-macroglobulins (alpha-Ms) covalently bind proteinases, which is accompanied by the exposure of carboxy terminal receptor recognition domains important for the rapid clearanc...

journal_title：Archives of biochemistry and biophysics

authors： Chiabrando GA,Vides MA,Sánchez MC

更新日期：2002-02-01 00:00:00

abstract：:A 1.1-kb Hp alpha I fragment of the Escherichia coli chromosome containing the gene for translation initiation factor 3 was employed as a probe in heterologous hybridization to chromosomal DNA from a variety of other procaryotes. Positive hybridization was observed to DNA derived from all gram-negative bacteria tested...

journal_title：Archives of biochemistry and biophysics

authors： Pramanik A,Schwartz I

更新日期：1984-11-15 00:00:00

abstract：:The present paper reports a quantitative investigation on the binding of aluminum to human serum albumin. Equilibrium dialysis and a general thermodynamic approach have been used to determine the binding parameters. Two aluminum binding sites have been identified on the albumin molecule, involving sites with single an...

journal_title：Archives of biochemistry and biophysics

authors： Zatta P,Dalla Via L,Di Noto V

更新日期：2003-09-01 00:00:00

abstract：:Interactions of six carbohydrates (trehalose, sucrose, glucose, raffinose, inositol, and glycerol) with dry dipalmitoylphosphatidylcholine (DPPC) were studied using differential scanning calorimetry (DSC) and infrared spectroscopy (ir) in order to elucidate the mechanism by which some of these carbohydrates preserve s...

journal_title：Archives of biochemistry and biophysics

authors： Crowe LM,Crowe JH,Chapman D

更新日期：1985-01-01 00:00:00

abstract：:Although an elastolytic activity in eosinophil-rich cell fractions from mice has been reported, this enzyme has not been purified and characterized as yet in any mammalian species. Eosinophilic elastase was isolated from human eosinophil fragments (cytosomes) obtained from normal and eosinophilic subjects. The enzyme ...

journal_title：Archives of biochemistry and biophysics

authors： Lungarella G,Menegazzi R,Gardi C,Spessotto P,de Santi MM,Bertoncin P,Patriarca P,Calzoni P,Zabucchi G

更新日期：1992-01-01 00:00:00

abstract：:The iodide oxidase activity and iodide-dependent pseudocatalatic activity of lignin peroxidase H2, an extracellular enzyme of the white rot fungus Phanerochaete chrysosporium, was inhibited by EDTA. The inhibition of iodide oxidase activity by EDTA was reversed at higher concentrations of iodide. Similar results were ...

journal_title：Archives of biochemistry and biophysics

authors： Shah MM,Aust SD

更新日期：1993-01-01 00:00:00

abstract：:Ca2+-regulation of cardiac contractility is mediated through the troponin complex, which comprises three subunits: cTnC, cTnI, and cTnT. As intracellular [Ca2+] increases, cTnI reduces its binding interactions with actin to primarily interact with cTnC, thereby enabling contraction. A portion of this regulatory switch...

journal_title：Archives of biochemistry and biophysics

authors： Bohlooli Ghashghaee N,Tanner BCW,Dong WJ

更新日期：2017-11-15 00:00:00

abstract：:The interaction of sarcoplasmic reticulum Ca(2+)-ATPase with the Mg.ATP analogues Rh(H2O)4ATP and Co(NH3)4ATP have been examined. Co(NH3)4ATP slowly inactivates Ca(2+)-ATPase in a first order process, with a rate constant of 1.13 x 10(-3) s-1 and an apparent inactivation constant, KI, of 32 mM. Rh(H2O)4ATP likewise in...

journal_title：Archives of biochemistry and biophysics

authors： Kuntzweiler TA,Grisham CM

更新日期：1992-05-15 00:00:00

abstract：:The fatty acid composition of total lipids and the adrenoceptor-mediated activation of glycogenolysis were studied in isolated hepatocytes from rats maintained on a control diet or on an essential fatty acid (EFA)-free diet. In cells from rats on the EFA-free diet there was a marked reduction in linoleic and arachidon...

journal_title：Archives of biochemistry and biophysics

authors： Grojec MS,Ishac EJ,Kapocsi J,Kunos G

更新日期：1990-11-15 00:00:00

abstract：:The Ah receptor from rat liver has been purified, using a specific oligonucleotide affinity column, in order to characterize the components of the receptor and to investigate features that modulate its DNA-binding activity. The purified DNA-binding form of rat Ah receptor contains three major components, with estimate...

journal_title：Archives of biochemistry and biophysics

authors： Ireland RC,Li SY,Dougherty JJ

更新日期：1995-06-01 00:00:00

abstract：:The effects of nalidixic acid and hydroxyurea on nuclear and chloroplast DNA formation in cultured cells of Nicotiana tabacum were investigated. At low concentrations (5 and 20 micrograms/ml) nalidixic acid, an inhibitor of DNA gyrase, exhibited a greater inhibitory effect on plastid DNA synthesis than on nuclear DNA ...

journal_title：Archives of biochemistry and biophysics

authors： Heinhorst S,Cannon G,Weissbach A

更新日期：1985-06-01 00:00:00

abstract：:This paper describes a theory for the mechanism of three-state transition of proteins which is often observed in aqueous organic cosolvent systems, i.e., from the native, via intermediate to helical forms. The first transition, accompanied by changes in the tertiary and/or secondary structures, was explained by larger...

journal_title：Archives of biochemistry and biophysics

authors： Arakawa T,Goddette D

更新日期：1985-07-01 00:00:00

abstract：:The effects of extracellular ATP on phosphoinositide metabolism and intracellular Ca2+ homeostasis were studied in Ehrlich ascites tumor cells. Cytosolic [Ca2+] was measured using either quin 2 or the recently described indicator fura 2. Addition of 0.5-25 microM extracellular ATP to intact cells results in a rapid mo...

journal_title：Archives of biochemistry and biophysics

authors： Dubyak GR

更新日期：1986-02-15 00:00:00

abstract：:Mammalian multidrug and toxic compound extrusion 1 (MATE1) are polyspecific H+-coupled exporters of organic cations (OCs) and responsible for excretion of metabolic waste products and xenobiotics. Here, we report a novel variant of mouse MATE1, mMATE1b, that has a long carboxyl terminal hydrophobic tail homologous to ...

journal_title：Archives of biochemistry and biophysics

authors： Kobara A,Hiasa M,Matsumoto T,Otsuka M,Omote H,Moriyama Y

更新日期：2008-01-15 00:00:00

abstract：:Previous studies in which dicyclohexylcarbodiimide (DCCD) was used to inactivate F1-ATPase enzymes have suggested that two glutamate residues in the beta-subunit are essential for catalysis. In the Escherichia coli F1-ATPase, these are residues beta-Glu-181 and beta-Glu-192. Oligonucleotide-directed mutagenesis was us...

journal_title：Archives of biochemistry and biophysics

authors： Parsonage D,Wilke-Mounts S,Senior AE

更新日期：1988-02-15 00:00:00

abstract：:The extrahepatic expression of UDP-glucuronosyltransferases (UGTs) is important in the detoxification of a number of endogenous and exogenous compounds, including 5-hydroxytryptamine and morphine. Studies were designed to investigate the extrahepatic expression of human UGTs using RT-PCR techniques and to determine th...

journal_title：Archives of biochemistry and biophysics

authors： King CD,Rios GR,Assouline JA,Tephly TR

更新日期：1999-05-01 00:00:00

abstract：:2-Isopropenyl-2-methyladamantane (2-PMADA) and 3-isopropenyl-3-methyldiamantane (3-PMDIA) showed potent and selective inhibition of cytochrome P450 (CYP) 2B6-mediated reactions with K(i) values of 5.27 and 2.17 microM, respectively. No effect on activities of other human CYP was found even at concentrations 100-fold h...

journal_title：Archives of biochemistry and biophysics

authors： Stiborová M,Borek-Dohalská L,Hodek P,Mráz J,Frei E

更新日期：2002-07-01 00:00:00

abstract：:We previously showed that knockdown of the anaplerotic enzyme pyruvate carboxylase in the INS-1 832/13 insulinoma cell line inhibited glucose-stimulated insulin release and glucose carbon incorporation into lipids. We now show that knockdown of fatty acid synthase (FAS) mRNA and protein also inhibits glucose-stimulate...

journal_title：Archives of biochemistry and biophysics

authors： MacDonald MJ,Hasan NM,Dobrzyn A,Stoker SW,Ntambi JM,Liu X,Sampath H

更新日期：2013-04-01 00:00:00

abstract：:Evidence indicates that activation of a beta 1----3N-acetylglucosaminyltransferase is responsible for accumulation of large quantities of lacto-series tumor-associated antigens in human colonic adenocarcinomas. Expression of type 1 and 2 core chain derivatives characterize human colonic adenocarcinomas, whereas normal...

journal_title：Archives of biochemistry and biophysics

authors： Holmes EH

更新日期：1989-05-01 00:00:00

abstract：:Histidinol dehydrogenase (EC 1.1.1.23) activity was determined in several plant species and in cultured plant cell lines. The enzyme was purified from cabbage (Brassica oleracea) to apparent homogeneity. To render complete purification, a new, specific histidinol-Sepharose 4B affinity chromatography was developed. The...

journal_title：Archives of biochemistry and biophysics

authors： Nagai A,Scheidegger A

更新日期：1991-01-01 00:00:00

abstract：:The reaction of the Cu,Co derivative of bovine Cu,Zn superoxide dismutase with phenylglyoxal or butanedione, which are known to inactivate the enzyme by selectively binding to Arg 141, has been studied by 1H NMR. Several 1H NMR lines of the copper-liganding histidine residues were perturbed, reproducing an effect so f...

journal_title：Archives of biochemistry and biophysics

authors： Paci M,Desideri A,Sette M,Rotilio G

更新日期：1991-04-01 00:00:00

abstract：:The chemical versatility of the flavin coenzyme is nearly unparalleled in enzyme catalysis. An interesting illustration of this versatility can be found in the reaction catalyzed by the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IDI-2) - an enzyme that interconverts the two essential isoprene...

journal_title：Archives of biochemistry and biophysics

authors： Thibodeaux CJ,Liu HW

更新日期：2017-10-15 00:00:00